ABCA2 (NM_212533) Human Untagged Clone

CAT#: SC308644

ABCA2 (untagged)-Human ATP-binding cassette, sub-family A (ABC1), member 2 (ABCA2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ABCA2
• Synonyms: ABC2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_212533, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGGAGAAAAACGAGCCGCGTGCAGCAGGGGCCGCCTCGGTCCCCTGCCTGCTCCGCTCACGGCGAGC
  GTTCCTGGGGCCTCGAGTTTCCCCCCTTCCGCTGGCTGCTCGGAATCGCGGGACGGACCCACCTGGCCGC
  CCTGCCTCCCTTCCAGTGGGTCCTGGCCTTCGAGATCTTCATCCCCCTGGTGCTGTTCTTTATCCTGCTG
  GGGCTGCGACAGAAGAAGCCCACCATCTCCGTGAAGGAAGTCTCCTTCTACACAGCGGCGCCCCTGACGT
  CTGCCGGCATCCTGCCTGTCATGCAATCGCTGTGCCCGGACGGCCAGCGAGACGAGTTCGGCTTCCTGCA
  GTACGCCAACTCCACGGTCACGCAGCTGCTTGAGCGCCTGGACCGCGTGGTGGAGGAAGGCAACCTGTTT
  GACCCAGCGCGGCCCAGCCTGGGCTCAGAGCTCGAGGCCCTACGCCAGCATCTGGAGGCCCTCAGTGCGG
  GCCCGGGCACCTCGGGGAGCCACCTGGACAGATCCACAGTGTCTTCCTTCTCTCTGGACTCGGTGGCCAG
  AAACCCGCAGGAGCTCTGGCGTTTCCTGACGCAAAACTTGTCGCTGCCCAATAGCACGGCCCAAGCACTC
  TTGGCCGCCCGTGTGGACCCGCCCGAGGTCTACCACCTGCTCTTTGGTCCCTCATCTGCCCTGGATTCAC
  AGTCTGGCCTCCACAAGGGTCAGGAGCCCTGGAGCCGCCTAGGGGGCAATCCCCTGTTCCGGATGGAGGA
  GCTGCTGCTGGCTCCTGCCCTCCTGGAGCAGCTCACCTGCACGCCGGGCTCGGGGGAGCTGGGCCGGATC
  CTCACTGTGCCTGAGAGTCAGAAGGGAGCCCTGCAGGGCTACCGGGATGCTGTCTGCAGTGGGCAGGCTG
  CTGCGCGTGCCAGGCGCTTCTCTGGGCTGTCTGCTGAGCTCCGGAACCAGCTGGACGTGGCCAAGGTCTC
  CCAGCAGCTGGGCCTGGATGCCCCCAACGGCTCGGACTCCTCGCCACAGGCGCCACCCCCACGGAGGCTG
  CAGGCGCTTCTGGGGGACCTGCTGGATGCCCAGAAGGTTCTGCAGGATGTGGATGTCCTGTCGGCCCTGG
  CCCTGCTACTGCCCCAGGGTGCCTGCACTGGCCGGACCCCCGGACCCCCAGCCAGTGGTGCGGGTGGGGC
  GGCCAATGGCACTGGGGCAGGGGCAGTCATGGGCCCCAACGCCACCGCTGAGGAGGGCGCACCCTCTGCT
  GCAGCACTGGCCACCCCGGACACGCTGCAGGGCCAGTGCTCAGCCTTCGTACAGCTCTGGGCCGGCCTGC
  AGCCCATCTTGTGTGGCAACAACCGCACCATTGAACCCGAGGCGCTGCGGCGGGGCAACATGAGCTCCCT
  GGGCTTCACGAGCAAGGAGCAGCGGAACCTGGGCCTCCTCGTGCACCTCATGACCAGCAACCCCAAAATC
  CTGTACGCGCCTGCGGGCTCTGAGGTCGACCGCGTCATCCTCAAGGCCAACGAGACTTTTGCTTTTGTGG
  GCAACGTGACTCACTATGCCCAGGTCTGGCTCAACATCTCGGCGGAGATCCGCAGCTTCCTGGAGCAGGG
  CAGGCTGCAGCAACACCTGCGCTGGCTGCAGCAGTATGTAGCAGAGCTGCGGCTGCACCCCGAGGCACTG
  AACCTGTCACTGGATGAGCTGCCGCCGGCCCTGAGACAGGACAACTTCTCGCTGCCCAGTGGCATGGCCC
  TCCTGCAGCAGCTGGATACCATTGACAACGCGGCCTGCGGCTGGATCCAGTTCATGTCCAAGGTGAGCGT
  GGACATCTTCAAGGGCTTCCCCGACGAGGAGAGCATTGTCAACTACACCCTCAACCAGGCCTACCAGGAC
  AACGTCACTGTTTTTGCCAGTGTGATCTTCCAGACCCGGAAGGACGGCTCGCTCCCGCCTCACGTGCACT
  ACAAGATCCGCCAGAACTCCAGCTTCACCGAGAAAACCAACGAGATCCGCCGCGCCTACTGGCGGCCTGG
  GCCCAATACTGGCGGCCGCTTCTACTTCCTCTACGGCTTCGTCTGGATCCAGGACATGATGGAGCGCGCC
  ATCATCGACACTTTTGTGGGGCACGATGTGGTGGAGCCAGGCAGCTACGTGCAGATGTTCCCCTACCCCT
  GCTACACACGCGATGACTTCCTGTTTGTCATTGAGCACATGATGCCGCTGTGCATGGTGATCTCCTGGGT
  CTACTCCGTGGCCATGACCATCCAGCACATCGTGGCGGAGAAGGAGCACCGGCTCAAGGAGGTGATGAAG
  ACCATGGGCCTGAACAACGCGGTGCACTGGGTGGCCTGGTTCATCACCGGCTTTGTGCAGCTGTCCATCT
  CCGTGACAGCACTCACCGCCATCCTGAAGTACGGCCAGGTGCTTATGCACAGCCACGTGGTCATCATCTG
  GCTCTTCCTGGCAGTCTACGCGGTGGCCACCATCATGTTCTGCTTCCTGGTGTCTGTGCTGTACTCCAAG
  GCCAAGCTGGCCTCGGCCTGCGGTGGCATCATCTACTTCCTGAGCTACGTGCCCTACATGTACGTGGCGA
  TCCGAGAGGAGGTGGCGCATGATAAGATCACGGCCTTCGAGAAGTGCATCGCGTCCCTCATGTCCACGAC
  GGCCTTTGGTCTGGGCTCTAAGTACTTCGCGCTGTATGAGGTGGCCGGCGTGGGCATCCAGTGGCACACC
  TTCAGCCAGTCCCCGGTGGAGGGGGACGACTTCAACTTGCTCCTGGCTGTCACCATGCTGATGGTGGACG
  CCGTGGTCTATGGCATCCTCACGTGGTACATTGAGGCTGTGCACCCAGGCATGTACGGGCTGCCCCGGCC
  CTGGTACTTCCCACTGCAGAAGTCCTACTGGCTGGGCAGTGGGCGGACAGAAGCCTGGGAGTGGAGCTGG
  CCGTGGGCACGCACCCCCCGCCTCAGTGTCATGGAGGAGGACCAGGCCTGTGCCATGGAGAGCCGGCGCT
  TTGAGGAGACCCGTGGCATGGAGGAGGAGCCCACCCACCTGCCTCTGGTTGTCTGCGTGGACAAACTCAC
  CAAGGTCTACAAGGACGACAAGAAGCTGGCCCTGAACAAGCTGAGCCTGAACCTCTACGAGAACCAGGTG
  GTCTCCTTCTTGGGCCACAACGGGGCGGGCAAGACCACCACCATGTCCATCCTGACCGGCCTGTTCCCTC
  CAACGTCGGGTTCCGCCACCATCTACGGGCACGACATCCGCACGGAGATGGATGAGATCCGCAAGAACCT
  GGGCATGTGCCCGCAGCACAATGTGCTCTTTGACCGGCTCACGGTGGAGGAACACCTCTGGTTCTACTCA
  CGGCTCAAGAGCATGGCTCAGGAGGAGATCCGCAGAGAGATGGACAAGATGATCGAGGACCTGGAGCTCT
  CCAACAAACGGCACTCACTGGTGCAGACATTGTCGGGTGGCATGAAGCGCAAGCTGTCCGTGGCCATCGC
  CTTCGTGGGCGGCTCTCGCGCCATCATCCTGGACGAGCCCACGGCGGGCGTGGACCCCTACGCGCGCCGC
  GCCATCTGGGACCTCATCCTGAAGTACAAGCCAGGCCGCACCATCCTTCTGTCCACCCACCACATGGATG
  AGGCTGACCTGCTTGGGGACCGCATTGCCATCATCTCCCATGGGAAGCTCAAGTGCTGCGGCTCCCCGCT
  CTTCCTCAAGGGCACCTATGGCGACGGGTACCGCCTCACGCTGGTCAAGCGGCCCGCCGAGCCGGGGGGC
  CCCCAAGAGCCAGGGCTGGCATCCAGCCCCCCAGGTCGGGCCCCGCTGAGCAGCTGCTCCGAGCTCCAGG
  TGTCCCAGTTCATCCGCAAGCATGTGGCCTCCTGCCTGCTGGTCTCAGACACAAGCACGGAGCTCTCCTA
  CATCCTGCCCAGCGAGGCCGCCAAGAAGGGGGCTTTCGAGCGCCTCTTCCAGCACCTGGAGCGCAGCCTG
  GATGCACTGCACCTCAGCAGCTTCGGGCTGATGGACACGACCCTGGAGGAAGTGTTCCTCAAGGTGTCGG
  AGGAGGATCAGTCGCTGGAGAACAGTGAGGCCGATGTGAAGGAGTCCAGGAAGGATGTGCTCCCTGGGGC
  GGAGGGCCCGGCGTCTGGGGAGGGTCACGCTGGCAATCTGGCCCGGTGCTCGGAGCTGACCCAGTCGCAG
  GCATCGCTGCAGTCGGCGTCATCTGTGGGCTCTGCCCGTGGCGACGAGGGAGCTGGCTACACCGACGTCT
  ATGGCGACTACCGCCCCCTCTTTGATAACCCACAGGACCCAGACAATGTCAGCCTGCAAGAGGTGGAGGC
  AGAGGCCCTGTCGAGGGTCGGCCAGGGCAGCCGCAAGCTGGACGGCGGGTGGCTGAAGGTGCGCCAGTTC
  CACGGGCTGCTGGTCAAACGCTTCCACTGCGCCCGCCGCAACTCCAAGGCACTCTTCTCCCAGATCTTGC
  TGCCAGCCTTCTTCGTCTGCGTGGCCATGACCGTGGCCCTGTCCGTCCCGGAGATTGGTGATCTGCCCCC
  GCTGGTCCTGTCACCTTCCCAGTACCACAACTACACCCAGCCCCGTGGCAATTTCATCCCCTACGCCAAC
  GAGGAGCGCCGCGAGTACCGGCTGCGGCTATCGCCCGACGCCAGCCCCCAGCAGCTCGTGAGCACGTTCC
  GGCTGCCGTCGGGGGTGGGTGCCACCTGCGTGCTCAAGTCTCCCGCCAACGGCTCGCTGGGGCCCACGTT
  GAACCTGAGCAGCGGGGAGTCGCGCCTGCTGGCGGCTCGGTTCTTCGACAGCATGTGTCTGGAGTCCTTC
  ACACAGGGGCTGCCACTGTCCAATTTCGTGCCACCCCCACCCTCGCCCGCCCCATCTGACTCGCCAGCGT
  CCCCGGATGAGGACCTGCAGGCCTGGAACGTCTCCCTGCCGCCCACCGCTGGGCCAGAAATGTGGACGTC
  GGCACCCTCCCTGCCGCGCCTGGTACGGGAGCCCGTCCGCTGCACCTGCTCTGCGCAGGGCACCGGCTTC
  TCCTGCCCCAGCAGTGTGGGCGGGCACCCGCCCCAGATGCGGGTGGTCACAGGCGACATCCTGACCGACA
  TCACCGGCCACAATGTCTCTGAGTACCTGCTCTTCACCTCCGACCGCTTCCGACTGCACCGGTATGGGGC
  CATCACCTTTGGAAACGTCCTGAAGTCCATCCCAGCCTCATTTGGCACCAGGGCCCCACCCATGGTGCGG
  AAGATCGCGGTGCGCAGGGCTGCCCAGGTTTTCTACAACAACAAGGGCTATCACAGCATGCCCACCTACC
  TCAACAGCCTCAACAACGCCATCCTGCGTGCCAACCTGCCCAAGAGCAAGGGCAACCCGGCGGCTTACGG
  CATCACCGTCACCAACCACCCCATGAATAAGACCAGCGCCAGCCTCTCCCTGGATTACCTGCTGCAGGGC
  ACGGATGTCGTCATCGCCATCTTCATCATCGTGGCCATGTCCTTCGTGCCGGCCAGCTTCGTTGTCTTCC
  TCGTGGCCGAGAAGTCCACCAAGGCCAAGCACCTGCAGTTTGTCAGCGGCTGCAACCCCATCATCTACTG
  GCTGGCGAACTACGTGTGGGACATGCTCAACTACCTGGTCCCCGCTACCTGCTGTGTCATCATCCTGTTT
  GTGTTCGACCTGCCGGCCTACACGTCGCCCACCAACTTCCCTGCCGTCCTCTCCCTCTTCCTGCTCTATG
  GGTGGTCCATCACGCCCATCATGTACCCGGCCTCCTTCTGGTTCGAGGTCCCCAGCTCCGCCTACGTGTT
  CCTCATTGTCATCAATCTCTTCATCGGCATCACCGCCACCGTGGCCACCTTCCTGCTACAGCTCTTCGAG
  CACGACAAGGACCTGAAGGTTGTCAACAGTTACCTGAAAAGCTGCTTCCTCATTTTCCCCAACTACAACC
  TGGGCCACGGGCTCATGGAGATGGCCTACAACGAGTACATCAACGAGTACTACGCCAAGATTGGCCAGTT
  TGACAAGATGAAGTCCCCGTTCGAGTGGGACATTGTCACCCGCGGACTGGTGGCCATGGCGGTTGAGGGC
  GTCGTGGGCTTCCTCCTGACCATCATGTGCCAGTACAACTTCCTGCGGCGGCCACAGCGCATGCCTGTGT
  CTACCAAGCCTGTGGAGGATGATGTGGACGTGGCCAGTGAGCGGCAGCGAGTGCTCCGGGGAGACGCCGA
  CAATGACATGGTCAAGATTGAGAACCTGACCAAGGTCTACAAGTCCCGGAAGATTGGCCGTATCCTGGCC
  GTTGACCGCCTGTGCCTGGGTGTGCGTCCTGGCGAGTGCTTCGGGCTCCTGGGCGTCAACGGTGCGGGCA
  AGACCAGCACCTTCAAGATGCTGACCGGCGACGAGAGCACGACGGGGGGCGAGGCCTTCGTCAATGGACA
  CAGCGTGCTGAAGGAGCTGCTCCAGGTGCAGCAGAGCCTCGGCTACTGCCCGCAGTGTGACGCGCTGTTC
  GACGAGCTCACGGCCCGGGAGCACCTGCAGCTGTACACGCGGCTGCGTGGGATCTCCTGGAAGGACGAGG
  CCCGGGTGGTGAAGTGGGCTCTGGAGAAGCTGGAGCTGACCAAGTACGCAGACAAGCCGGCTGGCACCTA
  CAGCGGCGGCAACAAGCGGAAGCTCTCCACGGCCATCGCCCTCATTGGGTACCCAGCCTTCATCTTCCTG
  GACGAGCCCACCACAGGCATGGACCCCAAGGCCCGGCGCTTCCTCTGGAACCTCATCCTTGACCTCATCA
  AGACAGGGCGTTCAGTGGTGCTGACATCACACAGCATGGAGGAGTGCGAGGCGCTGTGCACGCGGCTGGC
  CATCATGGTGAACGGTCGCCTGCGGTGCCTGGGCAGCATCCAGCACCTGAAGAACCGGTTTGGAGATGGC
  TACATGATCACGGTGCGGACCAAGAGCAGCCAGAGTGTGAAGGACGTGGTGCGGTTCTTCAACCGCAACT
  TCCCGGAAGCCATGCTCAAGGAGCGGCACCACACAAAGGTGCAGTACCAGCTCAAGTCGGAGCACATCTC
  GCTGGCCCAGGTGTTCAGCAAGATGGAGCAGGTGTCTGGCGTGCTGGGCATCGAGGACTACTCGGTCAGC
  CAGACCACACTGGACAATGTGTTCGTGAACTTTGCCAAGAAGCAGAGTGACAACCTGGAGCAGCAGGAGA
  CGGAGCCGCCATCCGCACTGCAGTCCCCTCTCGGCTGCTTGCTCAGCCTGCTCCGGCCCCGGTCTGCCCC
  CACGGAGCTCCGGGCACTTGTGGCAGACGAGCCCGAGGACCTGGACACGGAGGACGAGGGCCTCATCAGC
  TTCGAGGAGGAGCGGGCCCAGCTGTCCTTCAACACGGACACGCTCTGCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_212533
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_212533.2, NP_997698.1
• RefSeq Size: 8163 bp
• RefSeq ORF: 7401 bp
• Locus ID: 20
• Cytogenetics: 9q34.3
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters, Lysosome
• Gene Summary: 'The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intracellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the ABC1 subfamily. Members of the ABC1 subfamily comprise the only major ABC subfamily found exclusively in multicellular eukaryotes. This protein is highly expressed in brain tissue and may play a role in macrophage lipid metabolism and neural development. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) differs in the 5' UTR and coding region compared to variant 1. The resulting isoform (b) is longer and has a distinct N-terminus compared to isoform a. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene.