FGFR4 (NM_213647) Human Untagged Clone

CAT#: SC308692

FGFR4 (untagged)-Human fibroblast growth factor receptor 4 (FGFR4), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FGFR4
• Synonyms: CD334; JTK2; TKF
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_213647 edited
  ATGCGGCTGCTGCTGGCCCTGTTGGGGGTCCTGCTGAGTGTGCCTGGGCCTCCAGTCTTG
  TCCCTGGAGGCCTCTGAGGAAGTGGAGCTTGAGCCCTGCCTGGCTCCCAGCCTGGAGCAG
  CAAGAGCAGGAGCTGACAGTAGCCCTTGGGCAGCCTGTGCGGCTGTGCTGTGGGCGGGCT
  GAGCGTGGTGGCCACTGGTACAAGGAGGGCAGTCGCCTGGCACCTGCTGGCCGTGTACGG
  GGCTGGAGGGGCCGCCTAGAGATTGCCAGCTTCCTACCTGAGGATGCTGGCCGCTACCTC
  TGCCTGGCACGAGGCTCCATGATCGTCCTGCAGAATCTCACCTTGATTACAGGTGACTCC
  TTGACCTCCAGCAACGATGATGAGGACCCCAAGTCCCATAGGGACCTCTCGAATAGGCAC
  AGTTACCCCCAGCAAGCACCCTACTGGACACACCCCCAGCGCATGGAGAAGAAACTGCAT
  GCAGTACCTGCGGGGAACACCGTCAAGTTCCGCTGTCCAGCTGCAGGCAACCCCACGCCC
  ACCATCCGCTGGCTTAAGGATGGACAGGCCTTTCATGGGGAGAACCGCATTGGAGGCATT
  CGGCTGCGCCATCAGCACTGGAGTCTCGTGATGGAGAGCGTGGTGCCCTCGGACCGCGGC
  ACATACACCTGCCTGGTAGAGAACGCTGTGGGCAGCATCCGTTATAACTACCTGCTAGAT
  GTGCTGGAGCGGTCCCCGCACCGGCCCATCCTGCAGGCCGGGCTCCCGGCCAACACCACA
  GCCGTGGTGGGCAGCGACGTGGAGCTGCTGTGCAAGGTGTACAGCGATGCCCAGCCCCAC
  ATCCAGTGGCTGAAGCACATCGTCATCAACGGCAGCAGCTTCGGAGCCGACGGTTTCCCC
  TATGTGCAAGTCCTAAAGACTGCAGACATCAATAGCTCAGAGGTGGAGGTCCTGTACCTG
  CGGAACGTGTCAGCCGAGGACGCAGGCGAGTACACCTGCCTCGCAGGCAATTCCATCGGC
  CTCTCCTACCAGTCTGCCTGGCTCACGGTGCTGCCAGAGGAGGACCCCACATGGACCGCA
  GCAGCGCCCGAGGCCAGGTATACGGACATCATCCTGTACGCGTCGGGCTCCCTGGCCTTG
  GCTGTGCTCCTGCTGCTGGCCAGGCTGTATCGAGGGCAGGCGCTCCACGGCCGGCACCCC
  CGCCCGCCCGCCACTGTGCAGAAGCTCTCCCGCTTCCCTCTGGCCCGACAGTTCTCCCTG
  GAGTCAGGCTCTTCCGGCAAGTCAAGCTCATCCCTGGTACGAGGCGTGCGTCTCTCCTCC
  AGCGGCCCCGCCTTGCTCGCCGGCCTCGTGAGTCTAGATCTACCTCTCGACCCACTATGG
  GAGTTCCCCCGGGACAGGCTGGTGCTTGGGAAGCCCCTAGGCGAGGGCTGCTTTGGCCAG
  GTAGTACGTGCAGAGGCCTTTGGCATGGACCCTGCCCGGCCTGACCAAGCCAGCACTGTG
  GCCGTCAAGATGCTCAAAGACAACGCCTCTGACAAGGACCTGGCCGACCTGGTCTCGGAG
  ATGGAGGTGATGAAGCTGATCGGCCGACACAAGAACATCATCAACCTGCTTGGTGTCTGC
  ACCCAGGAAGGGCCCCTGTACGTGATCGTGGAGTGCGCCGCCAAGGGAAACCTGCGGGAG
  TTCCTGCGGGCCCGGCGCCCCCCAGGCCCCGACCTCAGCCCCGACGGTCCTCGGAGCAGT
  GAGGGGCCGCTCTCCTTCCCAGTCCTGGTCTCCTGCGCCTACCAGGTGGCCCGAGGCATG
  CAGTATCTGGAGTCCCGGAAGTGTATCCACCGGGACCTGGCTGCCCGCAATGTGCTGGTG
  ACTGAGGACAATGTGATGAAGATTGCTGACTTTGGGCTGGCCCGCGGCGTCCACCACATT
  GACTACTATAAGAAAACCAGCAACGGCCGCCTGCCTGTGAAGTGGATGGCGCCCGAGGCC
  TTGTTTGACCGGGTGTACACACACCAGAGTGACGTGTGGTCTTTTGGGATCCTGCTATGG
  GAGATCTTCACCCTCGGGGGCTCCCCGTATCCTGGCATCCCGGTGGAGGAGCTGTTCTCG
  CTGCTGCGGGAGGGACATCGGATGGACCGACCCCCACACTGCCCCCCAGAGCTGTACGGG
  CTGATGCGTGAGTGCTGGCACGCAGCGCCCTCCCAGAGGCCTACCTTCAAGCAGCTGGTG
  GAGGCGCTGGACAAGGTCCTGCTGGCCGTCTCTGAGGAGTACCTCGACCTCCGCCTGACC
  TTCGGACCCTATTCCCCCTCTGGTGGGGACGCCAGCAGCACCTGCTCCTCCAGCGATTCT
  GTCTTCAGCCACGACCCCCTGCCATTGGGATCCAGCTCCTTCCCCTTCGGGTCTGGGGTG
  CAGACATGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_213647 unedited
  TCTTGGGATTTGTAATCCCACTTACTATNAGGGCGGCCGAGCATTCGGCACGAGGCCGAG
  GAGCGCTCGGGCTGTCTGCGGACCCTGCCGCGTGCAGGGGTCGCGGCCGGCTGGAGCTGG
  GAGTGAGGCGGCGGAGGAGCCAGGTGAGGAGGAGCCAGGAAGGCAGTTGGTGGGAAGTCC
  AGCTTGGGTCCCTGAGAGCTGTGAGAAGGAGATGCTGTTTCTGCTGGCCCTGTTGGGGGT
  CCTGCTGAGTGTGCCTGGGCCTCCAGTCTTGTCCCTGGAGGCCTCTGAGGAAGTGGAGCT
  TGAGCCCTGCCTGGCTCCCAGCCTGGAGCAGCAAGAGCAGGAGCTGACAGTAGCCCTTGG
  GCAGCCTGTGCGGCTGTGCTGTGGGCGGGCTGAGCGTGGTGGCCACTGGTACAAGGAGGG
  CAGTCGCCTGGCACCTGCTGGCCGTGTACGGGGCTGGAGGGGCCGCCTAGAGATTGCCAG
  CTTCCTACCTGAGGATGCTGGCCGCTACCTCTGCCTGGCACGAGGCTCCATGATCGTCCT
  GCAGAATCTCACCTTGATTACAGGTGACTCCTTGACCTCCAGCAACGATGATGAGGACCC
  CAAGTCCCATAGGGACCTCTCGAATAGGCACAGTTACCCCCAGCAAGCACCCTACTGGAC
  ACACCCNCAGCGCATGGAGAAGAAACTGCATGCAGTACCTGCGGGGAACACCGTCAAGTT
  CCGCTGTCCAGCTGCATGCAACCCCACGCCCACCATCCGCTGGCTTATGGATGGACAGGC
  CTTTCATGGNGAGAACCGCATTGGATGCATTTCGCTGCNGCATCAGCACTGGAGTCTCGT
  GATGGANAGCGTGGTGCCCTCGGACCCGCGCACATACACCTGCCTGGTAGAGAACGCTGT
  GGGCAGCATCCGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_213647 unedited
  GGGCTTGGGAATATTTATAAAACGCCTTTGCTCCTGTTTTCGGCAGGCTTCCAGCTTCTC
  TGGGCTCAGGGGCCAATGCTCCCGTCAAGACGCTGGGGCAGCAGCAGCAGGGGGAGGTGT
  GGGGAAAGGGGGTTCAGAGGCCCAGAACCTCCTGCTGGTATTGGGAGGCAGGAGGTTTAG
  CATAGCAGCTCTCCAGCCAGGCTCAGCCAAACCCGGGATGGGGACTAAGCGCCAAGGTCC
  AAGAAGCCGAGCAGAACCCTGACATTTGGGCCATCAGGACACAGGCACGGCAGCTCCAAG
  GGCAAGGGCACGGCCCTTGGACACGGCACAGCAACTCTGGGCCAGGGGCCCCATGCTATC
  AAGGTCGAGCACTGTGTCAGGCTGTGGCTGAGCCCCAAGGCCCAAGGCCACCAGCCTATG
  TGCCTGCACAGCCTTGAGCCTTGCTCATGTCTGCACCCCAGACCCGAAGGGGAAGGAGCT
  GGATCCCAATGGCAGGGGGTCGTGGCTGAAGACAGAATCGCTGGAGGAGCAGGTGCTGCT
  GGCGTCCCCACCAGAGGGGGAATAGGGTCCGAAGGTCAGGCGGAGGTCGAGGTACTCCTC
  ANAGACGGCCAGCAGGACCTTGTCCAGCGCCTCCACCANCTGCTTGAAGGTANGCCTCTN
  GGAGGGCGCTGCGTGCCAGCACTCACGCATTANCCCCTACAGCTCTGGGGGGCAATGTGG
  GGGTCGGTCCATCCNATGTCCCTTCCGCAAGCATGAGGACAGCTCCTCACCCGGGTGCCT
  GAATCTGTGGATCCCCTCGGGTGATAATCTCCATTCCCGTTCCCAAAAACCCCCTTCCTC
  TGGTGGGTTCCCCCCGTAATAAAGGCTTCGGTGCCATCCCTTTACGGCATGGGCCTTCAT
  TTTTATTTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_213647
• Insert Size: 3240 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_213647.1, NP_998812.1
• RefSeq Size: 3084 bp
• RefSeq ORF: 2409 bp
• Locus ID: 2264
• Cytogenetics: 5q35.2
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Endocytosis, MAPK signaling pathway, Regulation of actin cytoskeleton
• Gene Summary: 'The protein encoded by this gene is a tyrosine kinase and cell surface receptor for fibroblast growth factors. The encoded protein is involved in the regulation of several pathways, including cell proliferation, cell differentiation, cell migration, lipid metabolism, bile acid biosynthesis, vitamin D metabolism, glucose uptake, and phosphate homeostasis. This protein consists of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment, and a cytoplasmic tyrosine kinase domain. The extracellular portion interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. [provided by RefSeq, Aug 2017]'
  Transcript Variant: This variant (3) uses an alternative splice site for the first exon compared to variant 1. Variants 1, 3 and 5 all encode the same isoform (1).