ATP7A (NM_000052) Human Untagged Clone

CAT#: SC308719

ATP7A (untagged)-Human ATPase, Cu++ transporting, alpha polypeptide (ATP7A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP7A
• Synonyms: DSMAX; MK; MNK; SMAX3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000052 edited
  CCGCCGCAGCCGCAGCTACTGTGACTTCTCCGATTGTGTGAGCTTTGTTGGAGCCTGCGT
  ACGTGGATTTATCGCTGCCACGGTCTGCGTAGCTCCAGAGGTTTAACCATAGGATAGAGA
  AACCAGGAATGTAATGAGGAAATCAAAATGGATCCAAGTATGGGTGTGAATTCTGTTACC
  ATTTCTGTTGAGGGTATGACTTGCAATTCCTGTGTTTGGACCATTGAGCAGCAGATTGGA
  AAAGTGAATGGTGTGCATCACATTAAGGTATCACTGGAAGAAAAAAATGCAACTATTATT
  TATGACCCTAAACTACAGACTCCAAAGACCCTACAGGAAGCTATTGATGACATGGGCTTT
  GATGCTGTTATCCATAATCCTGACCCTCTCCCTGTTTTAACTGACACCTTGTTTCTGACT
  GTTACGGCGTCACTGACTTTGCCATGGGACCATATCCAAAGCACATTGCTGAAGACCAAG
  GGTGTGACAGACATTAAAATTTACCCTCAGAAAAGAACTGTAGCAGTGACAATAATCCCT
  TCTATAGTGAATGCCAATCAGATAAAAGAGCTGGTTCCAGAACTCAGTTTAGATACTGGG
  ACACTGGAGAAAAAGTCAGGAGCTTGTGAAGATCATAGTATGGCTCAAGCTGGTGAAGTC
  GTGCTGAAGATGAAAGTGGAAGGGATGACCTGCCATTCATGTACTAGCACTATTGAAGGA
  AAAATTGGGAAACTGCAAGGTGTTCAGCGAATTAAAGTCTCCCTGGACAATCAAGAAGCT
  ACTATTGTTTATCAACCTCATCTTATCTCAGTAGAGGAAATGAAAAAGCAGATTGAAGCT
  ATGGGCTTTCCAGCATTTGTCAAAAAGCAGCCCAAGTACCTCAAATTGGGAGCTATTGAT
  GTAGAACGTCTAAAGAACACACCAGTTAAATCCTCAGAAGGGTCACAGCAAAGGAGTCCA
  TCATATACCAATGATTCAACAGCCACTTTCATCATTGATGGCATGCATTGTAAATCATGT
  GTGTCAAATATTGAAAGTACTTTATCTGCACTCCAATATGTAAGCAGCATAGTAGTTTCT
  TTAGAGAATAGGTCTGCCATTGTGAAGTATAATGCAAGCTCAGTCACTCCAGAATCCCTG
  AGAAAAGCAATAGAGGCTGTATCACCGGGGCTATATAGAGTTAGTATCACAAGTGAAGTT
  GAGAGTACCTCAAACTCTCCCTCCAGCTCATCTCTTCAGAAGATTCCTTTGAATGTAGTT
  AGCCAGCCTCTGACACAAGAAACTGTGATAAACATTGATGGCATGACTTGTAATTCCTGT
  GTGCAGTCTATTGAGGGTGTCATATCAAAAAAGCCAGGTGTAAAATCCATACGAGTCTCC
  CTTGCAAATAGCAATGGGACTGTTGAGTATGATCCTCTACTAACCTCTCCAGAAACGTTG
  AGAGGAGCAATAGAAGACATGGGATTTGATGCTACCTTGTCAGACACGAATGAGCCGTTG
  GTAGTAATAGCTCAGCCTTCATCGGAAATGCCGCTTTTGACTTCAACTAATGAATTTTAT
  ACTAAAGGGATGACACCAGTTCAAGACAAGGAGGAAGGAAAGAATTCATCTAAGTGTTAC
  ATACAGGTCACTGGCATGACTTGCGCTTCCTGTGTAGCAAACATTGAACGGAATTTAAGG
  CGGGAAGAAGGAATATATTCTATACTTGTGGCCCTGATGGCTGGCAAGGCAGAAGTAAGG
  TATAATCCTGCTGTTATACAACCCCCAATGATAGCAGAGTTCATCCGAGAACTTGGATTT
  GGAGCCACTGTGATAGAAAATGCTGATGAAGGAGATGGTGTTTTGGAACTTGTTGTGAGG
  GGAATGACGTGTGCCTCCTGCGTACATAAAATAGAGTCTAGTCTCACAAAACACAGAGGG
  ATCCTATACTGCTCCGTGGCCCTGGCAACCAACAAAGCACATATTAAATATGACCCAGAA
  ATTATTGGTCCTAGAGATATTATCCATACAATTGAAAGCTTAGGTTTTGAAGCTTCTTTG
  GTCAAGAAGGATCGGTCAGCAAGTCACTTAGATCATAAACGAGAAATAAGACAATGGAGA
  CGGTCTTTTCTTGTGAGTCTGTTTTTCTGTATTCCTGTAATGGGGCTGATGATATATATG
  ATGGTTATGGACCACCACTTTGCAACTCTTCACCATAATCAAAACATGAGTAAAGAAGAA
  ATGATCAACCTTCATTCTTCTATGTTCCTGGAGCGCCAGATTCTTCCAGGATTGTCTGTT
  ATGAATTTGCTGTCCTTTTTATTGTGTGTACCTGTACAGTTTTTCGGAGGCTGGTACTTC
  TACATTCAGGCTTATAAAGCACTGAAGCATAAGACAGCAAATATGGACGTACTGATTGTG
  CTGGCAACCACCATTGCATTTGCCTACTCTTTGATTATTCTTCTAGTTGCAATGTATGAG
  AGAGCCAAAGTGAACCCTATTACTTTCTTTGACACACCCCCTATGCTGTTTGTGTTTATT
  GCACTAGGCCGATGGCTGGAACATATAGCAAAGGGCAAAACATCAGAGGCTCTTGCAAAG
  TTAATTTCACTACAAGCTACAGAAGCAACTATTGTAACTCTTGATTCTGATAATATCCTC
  CTCAGTGAAGAACAAGTGGATGTGGAACTTGTACAACGTGGAGATATCATTAAAGTAGTT
  CCAGGAGGCAAATTTCCAGTGGATGGTCGTGTTATTGAAGGACATTCTATGGTAGATGAG
  TCCCTCATCACAGGGGAGGCAATGCCTGTGGCTAAGAAACCTGGCAGCACAGTGATTGCT
  GGTTCCATTAACCAGAACGGGTCACTGCTTATCTGCGCAACACATGTTGGAGCAGACACA
  ACCCTTTCTCAAATTGTCAAACTTGTGGAAGAGGCACAAACATCAAAGGCTCCTATCCAG
  CAGTTTGCAGACAAACTCAGTGGCTATTTTGTTCCTTTTATTGTTTTTGTTTCCATTGCC
  ACCCTCTTGGTATGGATTGTAATTGGATTTCTGAATTTTGAAATTGTGGAAACCTACTTT
  CCTGGCTACAATAGAAGTATCTCCCGAACAGAAACGATAATACGATTTGCTTTCCAAGCC
  TCTATCACAGTTCTGTGTATTGCATGTCCCTGTTCACTGGGACTGGCCACTCCAACTGCT
  GTGATGGTGGGTACAGGAGTAGGTGCTCAAAATGGCATACTAATAAAAGGTGGAGAGCCA
  TTGGAGATGGCTCATAAGGTAAAGGTAGTGGTATTTGATAAGACTGGAACCATTACTCAC
  GGAACCCCAGTGGTGAATCAAGTAAAGGTTCTAACTGAAAGTAACAGAATATCACACCAT
  AAAATCTTGGCCATTGTGGGAACTGCTGAAAGTAACAGTGAACACCCTCTAGGAACAGCC
  ATAACCAAATATTGCAAACAGGAGCTGGACACTGAAACCTTGGGTACCTGCATAGATTTC
  CAGGTTGTGCCAGGCTGTGGTATTAGCTGTAAAGTCACCAATATTGAAGGCTTGCTACAT
  AAGAATAACTGGAATATAGAGGACAATAATATTAAAAATGCATCCCTGGTTCAAATTGAT
  GCCAGTAATGAACAGTCATCAACTTCGTCTTCCATGATTATTGATGCCCAGATCTCAAAT
  GCTCTTAATGCTCAGCAGTATAAAGTCCTCATTGGTAACCGGGAGTGGATGATTAGAAAT
  GGTCTTGTCATTAATAACGATGTAAATGATTTCATGACTGAACATGAGAGAAAAGGTCGG
  ACTGCTGTATTAGTAGCAGTTGATGATGAGCTGTGTGGCTTGATAGCCATTGCAGACACA
  GTGAAGCCTGAAGCAGAACTGGCTATCCATATTCTGAAATCTATGGGCTTAGAAGTAGTT
  CTGATGACTGGAGACAACAGTAAAACAGCTAGATCTATTGCTTCTCAGGTTGGCATTACT
  AAGGTGTTTGCTGAAGTTCTACCTTCTCACAAGGTTGCTAAAGTGAAGCAACTTCAAGAG
  GAGGGGAAACGGGTAGCAATGGTGGGAGATGGAATCAATGACTCCCCAGCTCTGGCAATG
  GCTAATGTGGGAATTGCTATTGGCACAGGCACAGATGTAGCCATTGAAGCAGCTGATGTG
  GTTTTGATAAGGAATGATCTTCTGGATGTAGTGGCAAGTATTGACTTATCAAGAAAGACA
  GTCAAGAGGATTCGGATAAATTTTGTCTTTGCTCTAATTTATAATCTGGTTGGAATTCCC
  ATAGCTGCTGGAGTTTTTATGCCCATTGGTTTGGTTTTGCAGCCCTGGATGGGATCTGCA
  GCAATGGCTGCTTCATCTGTTTCTGTAGTACTTTCTTCTCTCTTCCTTAAACTTTACAGG
  AAACCAACTTACGAGAGTTATGAACTGCCTGCCCGGAGCCAGATAGGACAGAAGAGTCCT
  TCAGAAATCAGCGTTCATGTTGGAATAGATGATACCTCAAGGAATTCTCCTAAACTGGGT
  TTGCTGGACCGGATTGTTAATTATAGCAGAGCCTCTATAAACTCACTACTGTCTGATAAA
  CGCTCCCTAAACAGTGTTGTTACCAGTGAACCTGACAAGCACTCACTCCTGGTGGGAGAC
  TTCAGGGAAGATGATGACACTGCATTATAAAAGGCCATGGAGAGTGCTGCCAGTTTAACT
  TGTCATGCACTGACACAGCATTCATGATGTTACCTTCACTTTTCAAAATATTGTAGAAGG
  ATTTTTCTCATGCTCTTATATTAGGGATTCTATTTGAGTTGCGTTTATCTGTTGGCAAAA
  ATATCTTTTTCAAGGCATCAGCTCTGAACCTAGCTTTATTTAAACTGAATTTCCAGTATA
  TTTTTGTTTTCACTAACAACAGATAAGGTAGAGCAGTGAGGTTTACAACAAGCCCTACAA
  TTAGAGATTGCTGAACTGCTGCTAAAGTGATTTTTTTTTTATTTGACCAAAAAAAAAAAG
  GCCCAAGAAGAAGAAAATGAAAAATTTGAAGATTTGAGAGCATGAAGATATTCATGCTTT
  TGAACTCAAAATATTGAAGATACTCTCAAGCCTGTATCCCTGCCCACTGGGGAGCAATGA
  CTTTCAAAGCACTGTGTATAAAACATCTAGTTTTAGAAGGGAAACAGTTGAAACTGTTTA
  AAAATAGATGTGCCTTATTTATTGCAGGCTTTCTTTCCCCCATTCTCCCTGCATCCTTGT
  CCTTGCAGGTGCTTTTTTAGATGCTCCAATATGTCTTCTTTTGTTATTTTCTTTCGAGCT
  AACCAAGTTTAGGTGGTTTTTCATTGATTAAAAATAACTGACAACTGTTCTAATATTTTG
  CTCCTTTTTAAATTTTGTAGCTCAAAAGACCTTAAAGGTCTGTAGGGTTCCCTGCCTCCC
  ATCTTTCCACTGTTGTAAAAAGTATATCAAATTATTCCTTCAAGTTTCCTAGCTCTGTGC
  TCAGTTTCAGTTCACTCCTGCCAAGTTGGACTCTAAGTTATTCTTCATGTAGTCTGCTGA
  TCTCAGTCTGGAAACTTAACATTATGAGCCTTTTCTGCTCAAAAAATTTTCAAAGATTAA
  AACTATTATACATATACAGGTCATATAAAATTACCTGGATTCACTAAAAAAAAAAAAAAA
  AAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000052
• Insert Size: 5600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference. This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000052.2, NP_000043.2
• RefSeq Size: 8028 bp
• RefSeq ORF: 4503 bp
• Locus ID: 538
• Cytogenetics: Xq21.1
• Domains: E1-E2_ATPase, Hydrolase, HMA
• Protein Families: Druggable Genome, Transmembrane
• Gene Summary: 'This gene encodes a transmembrane protein that functions in copper transport across membranes. This protein is localized to the trans Golgi network, where it is predicted to supply copper to copper-dependent enzymes in the secretory pathway. It relocalizes to the plasma membrane under conditions of elevated extracellular copper, and functions in the efflux of copper from cells. Mutations in this gene are associated with Menkes disease, X-linked distal spinal muscular atrophy, and occipital horn syndrome. Alternatively-spliced transcript variants have been observed. [provided by RefSeq, Aug 2013]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.