ABCB4 (NM_018849) Human Untagged Clone

CAT#: SC308848

ABCB4 (untagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 4 (ABCB4), transcript variant B

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ABCB4
• Synonyms: ABC21; GBD1; ICP3; MDR2; MDR2/3; MDR3; PFIC-3; PGY3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018849, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATCTTGAGGCGGCAAAGAACGGAACAGCCTGGCGCCCCACGAGCGCGGAGGGCGACTTTGAACTGG
  GCATCAGCAGCAAACAAAAAAGGAAAAAAACGAAGACAGTGAAAATGATTGGAGTATTAACATTGTTTCG
  ATACTCCGATTGGCAGGATAAATTGTTTATGTCGCTGGGTACCATCATGGCCATAGCTCACGGATCAGGT
  CTCCCCCTCATGATGATAGTATTTGGAGAGATGACTGACAAATTTGTTGATACTGCAGGAAACTTCTCCT
  TTCCAGTGAACTTTTCCTTGTCGCTGCTAAATCCAGGCAAAATTCTGGAAGAAGAAATGACTAGATATGC
  ATATTACTACTCAGGATTGGGTGCTGGAGTTCTTGTTGCTGCCTATATACAAGTTTCATTTTGGACTTTG
  GCAGCTGGTCGACAGATCAGGAAAATTAGGCAGAAGTTTTTTCATGCTATTCTACGACAGGAAATAGGAT
  GGTTTGACATCAACGACACCACTGAACTCAATACGCGGCTAACAGATGACATCTCCAAAATCAGTGAAGG
  AATTGGTGACAAGGTTGGAATGTTCTTTCAAGCAGTAGCCACGTTTTTTGCAGGATTCATAGTGGGATTC
  ATCAGAGGATGGAAGCTCACCCTTGTGATAATGGCCATCAGCCCTATTCTAGGACTCTCTGCAGCCGTTT
  GGGCAAAGATACTCTCGGCATTTAGTGACAAAGAACTAGCTGCTTATGCAAAAGCAGGCGCCGTGGCAGA
  AGAGGCTCTGGGGGCCATCAGGACTGTGATAGCTTTCGGGGGCCAGAACAAAGAGCTGGAAAGGTATCAG
  AAACATTTAGAAAATGCCAAAGAGATTGGAATTAAAAAAGCTATTTCAGCAAACATTTCCATGGGTATTG
  CCTTCCTGTTAATATATGCATCATATGCACTGGCCTTCTGGTATGGATCCACTCTAGTCATATCAAAAGA
  ATATACTATTGGAAATGCAATGACAGTTTTTTTTTCAATCCTAATTGGAGCTTTCAGTGTTGGCCAGGCT
  GCCCCATGTATTGATGCTTTTGCCAATGCAAGAGGAGCAGCATATGTGATCTTTGATATTATTGATAATA
  ATCCTAAAATTGACAGTTTTTCAGAGAGAGGACACAAACCAGACAGCATCAAAGGGAATTTGGAGTTCAA
  TGATGTTCACTTTTCTTACCCTTCTCGAGCTAACGTCAAGATCTTGAAGGGCCTCAACCTGAAGGTGCAG
  AGTGGGCAGACGGTGGCCCTGGTTGGAAGTAGTGGCTGTGGGAAGAGCACAACGGTCCAGCTGATACAGA
  GGCTCTATGACCCTGATGAGGGCACAATTAACATTGATGGGCAGGATATTAGGAACTTTAATGTAAACTA
  TCTGAGGGAAATCATTGGTGTGGTGAGTCAGGAGCCGGTGCTGTTTTCCACCACAATTGCTGAAAATATT
  TGTTATGGCCGTGGAAATGTAACCATGGATGAGATAAAGAAAGCTGTCAAAGAGGCCAACGCCTATGAGT
  TTATCATGAAATTACCACAGAAATTTGACACCCTGGTTGGAGAGAGAGGGGCCCAGCTGAGTGGTGGGCA
  GAAGCAGAGGATCGCCATTGCACGTGCCCTGGTTCGCAACCCCAAGATCCTTCTGCTGGATGAGGCCACG
  TCAGCATTGGACACAGAAAGTGAAGCTGAGGTACAGGCAGCTCTGGATAAGGCCAGAGAAGGCCGGACCA
  CCATTGTGATAGCACACCGACTGTCTACGGTCCGAAATGCAGATGTCATCGCTGGGTTTGAGGATGGAGT
  AATTGTGGAGCAAGGAAGCCACAGCGAACTGATGAAGAAGGAAGGGGTGTACTTCAAACTTGTCAACATG
  CAGACATCAGGAAGCCAGATCCAGTCAGAAGAATTTGAACTAAATGATGAAAAGGCTGCCACTAGAATGG
  CCCCAAATGGCTGGAAATCTCGCCTATTTAGGCATTCTACTCAGAAAAACCTTAAAAATTCACAAATGTG
  TCAGAAGAGCCTTGATGTGGAAACCGATGGACTTGAAGCAAATGTGCCACCAGTGTCCTTTCTGAAGGTC
  CTGAAACTGAATAAAACAGAATGGCCCTACTTTGTCGTGGGAACAGTATGTGCCATTGCCAATGGGGGGC
  TTCAGCCGGCATTTTCAGTCATATTCTCAGAGATCATAGCGATTTTTGGACCAGGCGATGATGCAGTGAA
  GCAGCAGAAGTGCAACATATTCTCTTTGATTTTCTTATTTCTGGGAATTATTTCTTTTTTTACTTTCTTC
  CTTCAGGGTTTCACGTTTGGGAAAGCTGGCGAGATCCTCACCAGAAGACTGCGGTCAATGGCTTTTAAAG
  CAATGCTAAGACAGGACATGAGCTGGTTTGATGACCATAAAAACAGTACTGGTGCACTTTCTACAAGACT
  TGCCACAGATGCTGCCCAAGTCCAAGGAGCCACAGGAACCAGGTTGGCTTTAATTGCACAGAATATAGCT
  AACCTTGGAACTGGTATTATCATATCATTTATCTACGGTTGGCAGTTAACCCTATTGCTATTAGCAGTTG
  TTCCAATTATTGCTGTGTCAGGAATTGTTGAAATGAAATTGTTGGCTGGAAATGCCAAAAGAGATAAAAA
  AGAACTGGAAGCTGCTGGAAAGATTGCAACAGAGGCAATAGAAAATATTAGGACAGTTGTGTCTTTGACC
  CAGGAAAGAAAATTTGAATCAATGTATGTTGAAAAATTGTATGGACCTTACAGGAATTCTGTGCAGAAGG
  CACACATCTATGGAATTACTTTTAGTATCTCACAAGCATTTATGTATTTTTCCTATGCCGGTTGTTTTCG
  ATTTGGTGCATATCTCATTGTGAATGGACATATGCGCTTCAGAGATGTTATTCTGGTGTTTTCTGCAATT
  GTATTTGGTGCAGTGGCTCTAGGACATGCCAGTTCATTTGCTCCAGACTATGCTAAAGCTAAGCTGTCTG
  CAGCCCACTTATTCATGCTGTTTGAAAGACAACCTCTGATTGACAGCTACAGTGAAGAGGGGCTGAAGCC
  TGATAAATTTGAAGGAAATATAACATTTAATGAAGTCGTGTTCAACTATCCCACCCGAGCAAACGTGCCA
  GTGCTTCAGGGGCTGAGCCTGGAGGTGAAGAAAGGCCAGACACTAGCCCTGGTGGGCAGCAGTGGCTGTG
  GGAAGAGCACGGTGGTCCAGCTCCTGGAGCGGTTCTACGACCCCTTGGCGGGGACAGTGTTTGTGGACTT
  TGGTTTTCAGCTTCTCGATGGTCAAGAAGCAAAGAAACTCAATGTCCAGTGGCTCAGAGCTCAACTCGGA
  ATCGTGTCTCAGGAGCCTATCCTATTTGACTGCAGCATTGCCGAGAATATTGCCTATGGAGACAACAGCC
  GGGTTGTATCACAGGATGAAATTGTGAGTGCAGCCAAAGCTGCCAACATACATCCTTTCATCGAGACGTT
  ACCCCACAAATATGAAACAAGAGTGGGAGATAAGGGGACTCAGCTCTCAGGAGGTCAAAAACAGAGGATT
  GCTATTGCCCGAGCCCTCATCAGACAACCTCAAATCCTCCTGTTGGATGAAGCTACATCAGCTCTGGATA
  CTGAAAGTGAAAAGGTTGTCCAAGAAGCCCTGGACAAAGCCAGAGAAGGCCGCACCTGCATTGTGATTGC
  TCACCGCCTGTCCACCATCCAGAATGCAGACTTAATAGTGGTGTTTCAGAATGGGAGAGTCAAGGAGCAT
  GGCACGCATCAGCAGCTGCTGGCACAGAAAGGCATCTATTTTTCAATGGTCAGTGTCCAGGCTGGGACAC
  AGAACTTATGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_018849
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018849.2, NP_061337.1
• RefSeq Size: 3988 bp
• RefSeq ORF: 3861 bp
• Locus ID: 5244
• Cytogenetics: 7q21.12
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: ABC transporters
• Gene Summary: 'The membrane-associated protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MDR/TAP subfamily. Members of the MDR/TAP subfamily are involved in multidrug resistance as well as antigen presentation. This gene encodes a full transporter and member of the p-glycoprotein family of membrane proteins with phosphatidylcholine as its substrate. The function of this protein has not yet been determined; however, it may involve transport of phospholipids from liver hepatocytes into bile. Alternative splicing of this gene results in several products of undetermined function. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (B) uses an alternate in-frame splice site in the 3' coding region, compared to variant A, resulting in a longer protein (isoform B).