Phospholipase C epsilon 1 (PLCE1) (NM_016341) Human Untagged Clone

CAT#: SC308896

PLCE1 (untagged)-Human phospholipase C, epsilon 1 (PLCE1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PLCE1
• Synonyms: NPHS3; PLCE; PPLC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016341, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGACTTCTGAAGAAATGACAGCTTCTGTTCTCATACCTGTGACTCAGAGAAAAGTGGTTTCTGCCCAGT
  CGGCTGCAGATGAAAGTAGTGAAAAGGTCTCAGACATCAATATTTCAAAAGCACATACTGTCAGACGAAG
  TGGGGAGACTTCTCATACCATCTCACAACTGAACAAACTTAAAGAAGAACCTTCTGGAAGCAACTTGCCA
  AAGATTCTCTCAATAGCGAGGGAGAAAATAGTGAGTGATGAGAACAGTAATGAAAAATGTTGGGAGAAAA
  TCATGCCAGATTCTGCGAAAAACCTTAACATTAACTGCAACAACATATTGAGAAACCATCAGCATGGCCT
  TCCTCAGAGACAATTTTATGAAATGTACAACTCTGTTGCTGAGGAAGACTTGTGTTTAGAAACTGGAATT
  CCTTCTCCACTGGAAAGAAAGGTGTTCCCTGGAATTCAACTGGAACTAGACAGACCTTCCATGGGCATTA
  GTCCTTTAGGAAATCAGTCAGTGATCATAGAGACAGGCAGAGCACACCCTGACAGCAGAAGGGCAGTATT
  TCATTTTCATTATGAAGTTGACAGAAGAATGTCAGACACTTTCTGTACCCTATCAGAAAACTTAATTTTA
  GACGATTGTGGAAATTGTGTACCACTACCTGGGGGTGAGGAGAAGCAAAAGAAAAACTATGTGGCATATA
  CCTGTAAACTGATGGAATTGGCCAAAAATTGTGATAATAAGAATGAGCAGCTGCAGTGTGATCATTGTGA
  CACCTTGAATGATAAATACTTTTGCTTTGAAGGCTCTTGTGAGAAGGTTGACATGGTATATTCAGGTGAT
  AGCTTTTGTAGGAAAGACTTTACTGACAGTCAAGCTGCCAAGACCTTTTTGAGCCATTTTGAGGACTTCC
  CTGATAATTGTGATGATGTAGAAGAAGACGCTTTTAAAAGCAAAAAGGAGCGATCCACTTTGTTAGTCAG
  GAGATTCTGTAAAAATGACAGAGAAGTTAAGAAATCTGTGTATACTGGAACAAGAGCAATTGTGAGAACT
  CTGCCTTCTGGCCACATTGGGCTGACTGCATGGAGTTACATAGATCAGAAGAGAAATGGTCCCTTACTGC
  CTTGTGGGAGAGTAATGGAACCCCCGTCAACAGTGGAGATAAGGCAAGATGGGAGCCAACGTCTGTCAGA
  AGCCCAGTGGTATCCTATCTACAATGCAGTGAGAAGAGAAGAAACAGAAAATACAGTTGGATCTCTACTC
  CATTTCCTCACCAAGCTCCCAGCCTCCGAGACAGCCCATGGAAGGATAAGCGTTGGTCCATGCTTAAAGC
  AATGTGTCCGAGACACTGTATGTGAGTATCGCGCCACCCTCCAAAGGACTTCAATATCGCAGTACATCAC
  CGGTTCTCTCCTAGAAGCAACCACGTCTTTGGGAGCAAGAAGTGGCCTTCTCAGTACTTTTGGAGGATCC
  ACTGGACGAATGATGCTGAAAGAACGCCAGCCAGGCCCCTCTGTGGCCAATTCCAATGCCCTCCCTTCAA
  GTTCAGCTGGGATCAGCAAGGAGCTGATCGATCTGCAGCCTCTCATCCAGTTCCCAGAGGAAGTCGCCAG
  CATCCTGATGGAGCAAGAGCAGACTATTTACCGCAGGGTCTTGCCAGTCGACTACCTTTGCTTCTTAACA
  CGGGACTTGGGCACTCCTGAATGCCAGAGCTCCTTGCCCTGCCTCAAAGCATCCATCTCAGCGTCGATTC
  TTACCACTCAGAATGGAGAGCACAATGCCCTTGAAGATCTGGTGATGAGGTTTAATGAGGTGAGCTCCTG
  GGTGACATGGCTGATCCTCACGGCAGGCTCCATGGAGGAGAAGCGAGAAGTCTTTTCATATTTGGTGCAT
  GTGGCCAAATGCTGCTGGAACATGGGCAACTACAACGCTGTCATGGAGTTCTTGGCTGGCCTCAGGTCAA
  GAAAAGTTTTAAAAATGTGGCAGTTCATGGACCAGTCTGATATTGAGACCATGAGGAGCCTGAAGGATGC
  TATGGCCCAGCATGAGTCCTCTTGTGAGTACAGAAAGGTGGTGACACGTGCCCTGCACATCCCTGGCTGT
  AAGGTGGTTCCATTCTGTGGGGTGTTTCTGAAGGAGCTCTGTGAAGTGCTTGACGGCGCCTCCGGTCTCA
  TGAAGCTTTGCCCGCGGTACAATTCCCAAGAAGAAACTTTAGAGTTTGTAGCAGATTACAGTGGACAAGA
  TAATTTCTTACAACGAGTGGGACAAAATGGCTTAAAGAATTCGGAGAAGGAGTCCACTGTCAACAGCATC
  TTTCAGGTCATCCGGAGCTGCAATCGAAGTCTGGAGACAGACGAGGAGGACAGCCCCAGTGAAGGAAACA
  GCTCCAGGAAAAGCTCCTTGAAGGATAAAAGCCGATGGCAGTTTATAATTGGAGATTTGTTGGATTCAGA
  CAATGACATCTTTGAGCAATCCAAAGAATACGACTCTCATGGTTCAGAGGACTCACAGAAGGCCTTCGAC
  CATGGGACGGAGCTCATCCCTTGGTACGTGCTGTCCATCCAAGCCGATGTGCACCAGTTCCTGCTGCAGG
  GGGCCACGGTCATCCACTACGACCAGGACACACACCTCTCTGCCCGCTGCTTCCTCCAGCTTCAGCCCGA
  CAATAGCACCTTGACCTGGGTAAAGCCCACAACTGCCTCCCCAGCCAGCAGTAAAGCAAAACTTGGTGTA
  CTTAATAACACAGCTGAGCCTGGAAAATTCCCACTACTGGGTAATGCTGGATTAAGTAGCCTGACGGAAG
  GGGTCTTGGATCTTTTTGCAGTGAAGGCTGTATACATGGGCCACCCTGGCATTGATATACACACTGTGTG
  TGTTCAGAACAAACTGGGTAGCATGTTCCTGTCAGAGACTGGTGTGACATTGCTCTATGGGCTTCAGACC
  ACAGACAACAGATTATTGCACTTCGTGGCACCAAAGCACACAGCTAAAATGCTCTTCAGCGGATTATTGG
  AACTCACTAGAGCTGTGAGAAAGATGAGGAAATTCCCTGACCAAAGACAGCAGTGGCTGCGGAAACAGTA
  CGTCAGCCTTTATCAGGAGGATGGACGGTATGAAGGCCCAACTTTGGCTCACGCTGTGGAGTTGTTTGGT
  GGCAGACGGTGGAGTGCTCGAAACCCCAGCCCCGGAACATCAGCAAAGAATGCTGAGAAGCCCAATATGC
  AGAGAAACAATACCCTGGGCATAAGCACTACCAAGAAAAAGAAGAAAATCCTCATGAGGGGTGAGAGTGG
  AGAGGTAACTGACGATGAGATGGCAACCCGAAAGGCCAAGATGCACAAAGAGTGTCGAAGCCGGAGTGGT
  TCTGATCCTCAAGACATTAATGAACAAGAAGAATCAGAGGTGAATGCCATCGCTAACCCTCCAAACCCCC
  TCCCTTCCAGAAGAGCCCACTCTTTGACCACAGCTGGGTCCCCCAACTTGGCTGCCGGGACGTCATCTCC
  CATCAGGCCAGTGTCCTCCCCTGTGCTGTCTTCTTCAAACAAGAGCCCATCCAGTGCTTGGAGCAGTAGT
  AGCTGGCACGGGCGGATCAAAGGCGGCATGAAGGGATTTCAGAGCTTCATGGTTTCAGATAGCAACATGA
  GTTTTGTTGAATTTGTTGAGCTGTTCAAATCATTCAGTGTCAGGAGCCGCAAGGACCTGAAGGATCTGTT
  TGATGTCTATGCAGTGCCCTGCAACCGATCTGGCTCCGAGTCAGCCCCACTCTACACCAACCTGACAATT
  GATGAAAACACCAGCGATCTTCAGCCTGACCTAGATCTGTTGACCAGAAATGTCTCGGATTTGGGGTTGT
  TCATTAAGAGTAAACAGCAGCTATCGGACAACCAGAGGCAGATATCTGATGCCATTGCTGCTGCAAGCAT
  TGTGACAAATGGCACTGGGATTGAGAGCACATCTCTGGGCATTTTTGGGGTGGGCATACTTCAGCTCAAC
  GATTTCCTCGTGAATTGCCAAGGAGAACACTGCACTTATGATGAAATCCTCAGCATCATCCAGAAGTTCG
  AGCCTAGCATCAGTATGTGTCATCAGGGACTAATGTCATTTGAAGGGTTTGCCAGGTTTCTGATGGATAA
  AGAAAATTTTGCCTCAAAAAATGATGAGTCACAGGAGAACATTAAAGAACTGCAGCTACCCCTCTCATAC
  TATTACATCGAATCTTCGCACAATACCTACCTCACGGGCCATCAGCTCAAAGGAGAATCCTCGGTAGAAC
  TCTACAGCCAGGTCCTTTTGCAAGGCTGTCGAAGTGTAGAATTGGACTGCTGGGACGGAGACGATGGGAT
  GCCCATCATTTATCATGGACATACGCTGACAACCAAGATCCCCTTCAAGGAAGTGGTTGAAGCCATTGAT
  CGCAGTGCCTTCATCAACTCTGACCTGCCAATCATCATATCGATTGAGAACCACTGTTCATTGCCTCAGC
  AACGAAAAATGGCAGAAATTTTCAAGACTGTGTTTGGAGAAAAGCTGGTGACTAAATTCTTATTTGAGAC
  TGATTTCTCAGATGATCCAATGCTTCCTTCACCTGACCAACTCAGAAAGAAAGTTCTTCTTAAAAACAAG
  AAGCTAAAAGCCCATCAGACGCCAGTGGATATCTTAAAGCAAAAGGCTCATCAGTTAGCATCTATGCAAG
  TGCAGGCTTATAATGGTGGGAATGCCAACCCCCGACCTGCCAATAATGAGGAAGAGGAAGATGAGGAGGA
  CGAATATGATTATGACTATGAATCCCTTTCTGATGACAACATTCTGGAAGACAGACCTGAAAATAAATCA
  TGTAATGACAAGCTTCAGTTTGAATATAATGAAGAAATCCCAAAGAGGATAAAGAAAGCAGATAACTCTG
  CTTGCAACAAAGGAAAGGTTTATGATATGGAACTGGGAGAAGAATTTTATCTTGATCAGAATAAAAAGGA
  AAGCAGACAGATTGCACCAGAGCTTTCTGACCTTGTAATCTATTGTCAAGCAGTAAAATTTCCAGGACTG
  TCAACTCTAAATGCATCTGGCTCTAGCAGAGGAAAAGAAAGGAAAAGCAGGAAGTCCATTTTTGGCAACA
  ATCCGGGCAGAATGAGCCCAGGGGAGACAGCATCATTTAACAAAACATCTGGAAAAAGTTCCTGTGAAGG
  CATTCGACAGACCTGGGAGGAATCTTCTTCCCCTCTCAACCCAACCACGTCCCTCAGTGCTATCATTAGA
  ACTCCCAAATGTTATCATATCTCGTCGCTGAATGAAAATGCCGCCAAACGTCTGTGTCGCAGGTATTCTC
  AGAAACTGACCCAGCACACCGCCTGTCAGCTGCTGAGAACTTACCCTGCTGCCACCCGCATCGACTCTTC
  CAACCCGAACCCCCTCATGTTCTGGCTCCATGGGATACAGCTTGTGGCACTCAACTACCAGACTGATGAT
  CTCCCTTTACATTTAAATGCTGCAATGTTTGAGGCAAATGGTGGTTGTGGTTATGTATTGAAACCTCCAG
  TTCTGTGGGACAAGAACTGCCCCATGTATCAGAAGTTTTCTCCACTAGAAAGAGATCTGGACAGCATGGA
  TCCTGCAGTCTATTCTTTAACTATTGTCTCTGGTCAGAATGTGTGCCCCAGTAATAGCATGGGAAGCCCG
  TGCATTGAAGTCGACGTCCTGGGCATGCCTCTGGACAGCTGCCATTTCCGCACAAAGCCCATCCATCGAA
  ACACCCTGAACCCCATGTGGAACGAGCAGTTTCTGTTCCACGTTCACTTCGAAGATCTTGTATTTCTTCG
  TTTTGCAGTTGTGGAAAACAATAGTTCAGCGGTAACTGCTCAGAGAATCATTCCACTGAAAGCTTTAAAA
  CGAGGATATCGACATCTTCAGCTGCGAAACCTTCACAATGAAGTCTTGGAGATTTCTAGTTTATTCATTA
  ACAGCAGAAGGATGGAAGAAAATTCCTCTGGCAATACCATGTCAGCCTCTTCGATGTTTAATACAGAAGA
  AAGAAAATGTTTGCAGACTCACAGAGTCACGGTGCATGGGGTCCCAGGGCCAGAGCCCTTTACCGTTTTC
  ACTATTAATGGAGGCACCAAGGCAAAGCAGCTTCTGCAGCAAATTCTGACAAATGAACAAGACATCAAAC
  CTGTTACCACAGACTATTTTTTGATGGAAGAAAAATATTTTATATCTAAAGAAAAGAATGAATGTAGGAA
  ACAACCATTCCAGAGAGCCATTGGTCCAGAAGAGGAGATCATGCAAATTTTAAGCAGCTGGTTTCCAGAA
  GAGGGATACATGGGCAGGATTGTCTTAAAAACCCAGCAGGAAAACCTAGAAGAGAAAAACATTGTTCAAG
  ATGACAAAGAGGTGATCTTGAGCTCAGAGGAGGAGAGTTTCTTTGTCCAAGTGCATGATGTTTCTCCAGA
  GCAACCTCGAACAGTCATCAAAGCACCCCGCGTCAGCACTGCACAGGATGTCATTCAGCAGACCTTATGC
  AAAGCCAAATATTCCTACAGCATCCTGAGCAACCCCAATCCAAGCGACTATGTGCTTTTGGAAGAGGTGG
  TGAAAGACACTACCAACAAGAAGACTACCACACCAAAGTCCTCTCAGCGGGTCCTTCTGGATCAGGAGTG
  TGTGTTTCAAGCCCAAAGCAAGTGGAAAGGTGCAGGAAAATTCATCCTTAAGCTAAAGGAGCAGGTGCAG
  GCATCTCGAGAAGATAAAAAGAAAGGCATTTCTTTCGCAAGTGAACTCAAGAAGCTCACCAAGTCAACTA
  AACAGCCCCGAGGACTTACATCACCTTCTCAGCTCTTGACCTCAGAAAGTATCCAAACCAAGGAGGAGAA
  ACCTGTGGGTGGCTTGTCCTCCAGTGACACAATGGATTACCGACAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_016341
• ORF Size: 6909 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_016341.3, NP_057425.3
• RefSeq Size: 7992
• RefSeq ORF: 6909
• Locus ID: 51196
• Domains: C2, RA, PI-PLC-X, PI-PLC-Y, RasGEF
• Protein Families: Druggable Genome
• Protein Pathways: Calcium signaling pathway, Inositol phosphate metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• Gene Summary: This gene encodes a phospholipase enzyme that catalyzes the hydrolysis of phosphatidylinositol-4,5-bisphosphate to generate two second messengers: inositol 1,4,5-triphosphate (IP3) and diacylglycerol (DAG). These second messengers subsequently regulate various processes affecting cell growth, differentiation, and gene expression. This enzyme is regulated by small monomeric GTPases of the Ras and Rho families and by heterotrimeric G proteins. In addition to its phospholipase C catalytic activity, this enzyme has an N-terminal domain with guanine nucleotide exchange (GEF) activity. Mutations in this gene cause early-onset nephrotic syndrome; characterized by proteinuria, edema, and diffuse mesangial sclerosis or focal and segmental glomerulosclerosis. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Sep 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1; also known as PLCepsilon1a).