PARP2 (NM_005484) Human Untagged Clone

CAT#: SC308977

PARP2 (untagged)-Human poly (ADP-ribose) polymerase 2 (PARP2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PARP2
• Synonyms: ADPRT2; ADPRTL2; ADPRTL3; ARTD2; pADPRT-2; PARP-2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005484 edited
  ATGGCGGCGCGGCGGCGACGGAGCACCGGCGGCGGCAGGGCGAGAGCATTAAATGAAAGC
  AAAAGAGTTAATAATGGCAACACGGCTCCAGAAGACTCTTCCCCTGCCAAGAAAACTCGT
  AGATGCCAGAGACAGGAGTCGAAAAAGATGCCTGTGGCTGGAGGAAAAGCTAATAAGGAC
  AGGACAGAAGACAAGCAAGATGGTATGCCAGGAAGGTCATGGGCCAGCAAAAGGGTCTCT
  GAATCTGTGAAGGCCTTGCTGTTAAAGGGCAAAGCTCCTGTGGACCCAGAGTGTACAGCC
  AAGGTGGGGAAGGCTCATGTGTATTGTGAAGGAAATGATGTCTATGATGTCATGCTAAAT
  CAGACCAATCTCCAGTTCAACAACAACAAGTACTATCTGATTCAGCTATTAGAAGATGAT
  GCCCAGAGGAACTTCAGTGTTTGGATGAGATGGGGCCGAGTTGGGAAAATGGGACAGCAC
  AGCCTGGTGGCTTGTTCAGGCAATCTCAACAAGGCCAAGGAAATCTTTCAGAAGAAATTC
  CTTGACAAAACGAAAAACAATTGGGAAGATCGAGAAAAGTTTGAGAAGGTGCCTGGAAAA
  TATGATATGCTACAGATGGACTATGCCACCAATACTCAGGATGAAGAGGAAACAAAGAAA
  GAGGAATCTCTTAAATCTCCCTTGAAGCCAGAGTCACAGCTAGATCTTCGGGTACAGGAG
  TTAATAAAGTTGATCTGTAATGTTCAGGCCATGGAAGAAATGATGATGGAAATGAAGTAT
  AATACCAAGAAAGCCCCACTTGGGAAGCTGACAGTGGCACAAATCAAGGCAGGTTACCAG
  TCTCTTAAGAAGATTGAGGATTGTATTCGGGCTGGCCAGCATGGACGAGCTCTCATGGAA
  GCATGCAATGAATTCTACACCAGGATTCCGCATGACTTTGGACTCCGTACTCCTCCACTA
  ATCCGGACACAGAAGGAACTGTCAGAAAAAATACAATTACTAGAGGCTTTGGGAGACATT
  GAAATTGCTATTAAGCTGGTGAAAACAGAGCTACAAAGCCCAGAACACCCATTGGACCAA
  CACTATAGAAACCTACATTGTGCCTTGCGCCCCCTTGACCATGAAAGTTACGAGTTCAAA
  GTGATTTCCCAGTACCTACAATCTACCCATGCTCCCACACACAGCGACTATACCATGACC
  TTGCTGGATTTGTTTGAAGTGGAGAAGGATGGTGAGAAAGAAGCCTTCAGAGAGGACCTT
  CATAACAGGATGCTTCTATGGCATGGTTCCAGGATGAGTAACTGGGTGGGAATCTTGAGC
  CATGGGCTTCGAATTGCCCCACCTGAAGCTCCCATCACAGGTTACATGTTTGGGAAAGGA
  ATCTACTTTGCTGACATGTCTTCCAAGAGTGCCAATTACTGCTTTGCCTCTCGCCTAAAG
  AATACAGGACTGCTGCTCTTATCAGAGGTAGCTCTAGGTCAGTGTAATGAACTACTAGAG
  GCCAATCCTAAGGCCGAAGGATTGCTTCAAGGTAAACATAGCACCAAGGGGCTGGGCAAG
  ATGGCTCCCAGTTCTGCCCACTTCGTCACCCTGAATGGGAGTACAGTGCCATTAGGACCA
  GCAAGTGACACAGGAATTCTGAATCCAGATGGTTATACCCTCAACTACAATGAATATATT
  GTATATAACCCCAACCAGGTCCGTATGCGGTACCTTTTAAAGGTTCAGTTTAATTTCCTT
  CAGCTGTGGTGAATGTTGATATTAAATAAACCAGAGATCTGATCTTCAAGCAAGAAAATA
  AGCAGTGTTGTACTTGTGAATTTTGTGATATTTTATGTAATAAAAACTGTACAGGTCTAA
  AAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_005484
• ORF Size: 1605 bp
• Insert Size: 2200
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_005484.2. This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene.

Reference Data

• RefSeq: NM_005484.2, NP_005475.1
• RefSeq Size: 1887
• RefSeq ORF: 1605
• Locus ID: 10038
• Domains: PARP, PARP_reg
• Protein Families: Druggable Genome
• Protein Pathways: Base excision repair
• Gene Summary: This gene encodes poly(ADP-ribosyl)transferase-like 2 protein, which contains a catalytic domain and is capable of catalyzing a poly(ADP-ribosyl)ation reaction. This protein has a catalytic domain which is homologous to that of poly (ADP-ribosyl) transferase, but lacks an N-terminal DNA binding domain which activates the C-terminal catalytic domain of poly (ADP-ribosyl) transferase. The basic residues within the N-terminal region of this protein may bear potential DNA-binding properties, and may be involved in the nuclear and/or nucleolar targeting of the protein. Two alternatively spliced transcript variants encoding distinct isoforms have been found. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes a longer isoform (1).