PPAR alpha (PPARA) (NM_005036) Human Untagged Clone

CAT#: SC308984

PPARA (untagged)-Human peroxisome proliferator-activated receptor alpha (PPARA), transcript variant 5

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPARA
• Synonyms: hPPAR; NR1C1; PPAR; PPARalpha
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_005036 edited
  ATGGTGGACACGGAAAGCCCACTCTGCCCCCTCTCCCCACTCGAGGCCGGCGATCTAGAG
  AGCCCGTTATCTGAAGAGTTCCTGCAAGAAATGGGAAACATCCAAGAGATTTCGCAATCC
  ATCGGCGAGGATAGTTCTGGAAGCTTTGGCTTTACGGAATACCAGTATTTAGGAAGCTGT
  CCTGGCTCAGATGGCTCGGTCATCACGGACACGCTTTCACCAGCTTCGAGCCCCTCCTCG
  GTGACTTATCCTGTGGTCCCCGGCAGCGTGGACGAGTCTCCCAGTGGAGCATTGAACATC
  GAATGTAGAATCTGCGGGGACAAGGCCTCAGGCTATCATTACGGAGTCCACGCGTGTGAA
  GGCTGCAAGGGCTTCTTTCGGCGAACGATTCGACTCAAGCTGGTGTATGACAAGTGCGAC
  CGCAGCTGCAAGATCCAGAAAAAGAACAGAAACAAATGCCAGTATTGTCGATTTCACAAG
  TGCCTTTCTGTCGGGATGTCACACAACGCGATTCGTTTTGGACGAATGCCAAGATCTGAG
  AAAGCAAAACTGAAAGCAGAAATTCTTACCTGTGAACATGACATAGAAGATTCTGAAACT
  GCAGATCTCAAATCTCTGGCCAAGAGAATCTACGAGGCCTACTTGAAGAACTTCAACATG
  AACAAGGTCAAAGCCCGGGTCATCCTCTCAGGAAAGGCCAGTAACAATCCACCTTTTGTC
  ATACATGATATGGAGACACTGTGTATGGCTGAGAAGACGCTGGTGGCCAAGCTGGTGGCC
  AATGGCATCCAGAACAAGGAGGCGGAGGTCCGCATCTTTCACTGCTGCCAGTGCACGTCA
  GTGGAGACCGTCACGGAGCTCACGGAATTCGCCAAGGCCATCCCAGGCTTCGCAAACTTG
  GACCTGAACGATCAAGTGACATTGCTAAAATACGGAGTTTATGAGGCCATATTCGCCATG
  CTGTCTTCTGTGATGAACAAAGACGGGATGCTGGTAGCGTATGGAAATGGGTTTATAACT
  CGTGAATTCCTAAAAAGCCTAAGGAAACCGTTCTGTGATATCATGGAACCCAAGTTTGAT
  TTTGCCATGAAGTTCAATGCACTGGAACTGGATGACAGTGATATCTCCCTTTTTGTGGCT
  GCTATCATTTGCTGTGGAGATCGTCCTGGCCTTCTAAACGTAGGACACATTGAAAAAATG
  CAGGAGGGTATTGTACATGTGCTCAGACTCCACCTGCAGAGCAACCACCCGGACGATATC
  TTTCTCTTCCCAAAACTTCTTCAAAAAATGGCAGACCTCCGGCAGCTGGTGACGGAGCAT
  GCGCAGCTGGTGCAGATCATCAAGAAGACGGAGTCGGATGCTGCGCTGCACCCGCTACTG
  CAGGAGATCTACAGGGACATGTACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005036 unedited
  NCCCGTTCAAATTTTGTATACGACTCACTAAAGGCGGCCGCGAATATCGCACGAAGAGCA
  GATTCAGTGTCATGTGAAGACGTGCTTCCTTGCTTCATAGATAAGAGTGCCACACCCCCC
  TGTCCCAGTGCACTGGGGCTGTGGATCCCTTCAAAGCTGAGATTGCCTGTCTGTGGTCTC
  CAGCGTTAAGCACAGTCATTAGCTCAGCTTGGAGCTCGGCGGCACAACCAGCACCATCTG
  GTCGCGATGGTGGACACGGAAAGCCCACTTCTTGCCCCCTCTCCCCACTCGAGGCCGGCG
  ATCTAGAGAGCCCGTTATCTGAAGAGTTCCTGCAAGAAATGGGAAACATCCAAGAGATTT
  CGCAATCCATCGGCAAGGATAGTTCTGGAAGCTTTGGCTTTACGGAATACCAGTATTTAG
  GAAAGCTGTCCTGGTNCAGATGGGCTCGGTCATCACGGACACGCTTTCACCAGCTTCGAG
  CCCTCCCTCGGTGACTATNCTTGTGGTCCCCGGCAGCGTGGACGAGTCTCCCAGTGGAGC
  ATTGAACATCGAATGTAGAATCTGCGGGGACAAGGCCTCAGGCTATCATTAACGGAGTCC
  ACGCGTGGTGAAGGCTGCAAGGGCCTTCTTTCGGCGAACGATTCGACTCAGCCTGGNNGT
  ATGAACAAGTGCGACCGCAGCTGGCNAGATCCAGAAAAAGAACAGAACANATGCCAATTA
  TGGGGNCGATTTCACAGTGCCTTTTTTGGGGGGGGGGATGTCACACACCCAAATTTTTTT
  TTTGGAACGATGCCCCAAAATTTTGAGAAAGCCAAACTGAAAGCAGAAATTCTTTACCTG
  TGACATGAATATTNAAAGATTCTGGACCTTGCAGATCTAAAAATCTCTGCCA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005036 unedited
  ATGGGGTAATTCGTTAAGTACACCGATCNAGNGAANNNGAAGCNANGCGGAAGCANGGNG
  ATATAAGATGATGAAAAAAAAACTCTGGTAAAGCAATATACGAATAAGTCTGATATAAGT
  GGGACTACTGAGTGTGCCACCGGAGAACTATTTTATAATTTTGTGACAGACAGGGTATTG
  CTAGCCGCCTATGATGATTTGAAGGATGGGCTAAGTGATGGGGCCATCTTGGCCTCCAAA
  AGTGCTGGGATTACATGTGTGAGCCAGTGTGCCTAGGCTGATTACAAAAACCAAAACGGA
  ATAAATTTGTAATTTTTAAAAAAGGATGTTCTAACCATAAGTTTCACTTTTTTCTAGTGG
  AAGTTTNACACTGTGTCCTTACAACTCTAATATTTTTATAATGCTGGTCATGGGACCGTT
  ATAAATCCTAAAGATTGTTAAAATAATAATGACGTGAGAAGAGACAAATTTTATAAAGAT
  GAGGGGAGAGGAATTAAAGATACTTTGAGGTGCAGAGAGATTCCCTGGAGGAGGAGGTCC
  GGGGAGTTCCAGAGCAGGATCTAAAAAGGTTTAAGAGGAAAGTTTATATACACAGAGGAC
  CAAGAGAGAAAGTGGTATAAGGTGAATAGTTTTCTTGACAAAATAGGATAATAAAGGGTG
  ACTTCCCAACTGGGACAGAAGGGGGTAAGTTGGGTTTGAAGGCTAAAAAAAAAGTTCACA
  TTTTGTAAAAACTCGCTCACCACCAAGGAGACTAAAAAACCTAAACCACTGAAAAAAAAA
  CCCAAACTCGGGAAATGTGAAATATAAAGAAAACAGCCCAGTGAGGAAAATCGGCCCCCC
  TCCCCCGCCCCCCGCCCCACGCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005036
• Insert Size: 4200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005036.4, NP_005027.2
• RefSeq Size: 10049 bp
• RefSeq ORF: 1407 bp
• Locus ID: 5465
• Cytogenetics: 22q13.31
• Domains: HOLI, zf-C4
• Protein Families: Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
• Protein Pathways: Adipocytokine signaling pathway, PPAR signaling pathway
• Gene Summary: 'Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (5) represents the longer transcript. Variants 3 and 5 encode the same protein.