PPAR alpha (PPARA) (NM_005036) Human Untagged Clone

CAT#: SC308984

PPARA (untagged)-Human peroxisome proliferator-activated receptor alpha (PPARA), transcript variant 5


  "NM_005036" in other vectors (6)

Reconstitution Protocol

USD 780.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol PPARA
Synonyms hPPAR; NR1C1; PPAR; PPARalpha
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_005036 edited
ATGGTGGACACGGAAAGCCCACTCTGCCCCCTCTCCCCACTCGAGGCCGGCGATCTAGAG
AGCCCGTTATCTGAAGAGTTCCTGCAAGAAATGGGAAACATCCAAGAGATTTCGCAATCC
ATCGGCGAGGATAGTTCTGGAAGCTTTGGCTTTACGGAATACCAGTATTTAGGAAGCTGT
CCTGGCTCAGATGGCTCGGTCATCACGGACACGCTTTCACCAGCTTCGAGCCCCTCCTCG
GTGACTTATCCTGTGGTCCCCGGCAGCGTGGACGAGTCTCCCAGTGGAGCATTGAACATC
GAATGTAGAATCTGCGGGGACAAGGCCTCAGGCTATCATTACGGAGTCCACGCGTGTGAA
GGCTGCAAGGGCTTCTTTCGGCGAACGATTCGACTCAAGCTGGTGTATGACAAGTGCGAC
CGCAGCTGCAAGATCCAGAAAAAGAACAGAAACAAATGCCAGTATTGTCGATTTCACAAG
TGCCTTTCTGTCGGGATGTCACACAACGCGATTCGTTTTGGACGAATGCCAAGATCTGAG
AAAGCAAAACTGAAAGCAGAAATTCTTACCTGTGAACATGACATAGAAGATTCTGAAACT
GCAGATCTCAAATCTCTGGCCAAGAGAATCTACGAGGCCTACTTGAAGAACTTCAACATG
AACAAGGTCAAAGCCCGGGTCATCCTCTCAGGAAAGGCCAGTAACAATCCACCTTTTGTC
ATACATGATATGGAGACACTGTGTATGGCTGAGAAGACGCTGGTGGCCAAGCTGGTGGCC
AATGGCATCCAGAACAAGGAGGCGGAGGTCCGCATCTTTCACTGCTGCCAGTGCACGTCA
GTGGAGACCGTCACGGAGCTCACGGAATTCGCCAAGGCCATCCCAGGCTTCGCAAACTTG
GACCTGAACGATCAAGTGACATTGCTAAAATACGGAGTTTATGAGGCCATATTCGCCATG
CTGTCTTCTGTGATGAACAAAGACGGGATGCTGGTAGCGTATGGAAATGGGTTTATAACT
CGTGAATTCCTAAAAAGCCTAAGGAAACCGTTCTGTGATATCATGGAACCCAAGTTTGAT
TTTGCCATGAAGTTCAATGCACTGGAACTGGATGACAGTGATATCTCCCTTTTTGTGGCT
GCTATCATTTGCTGTGGAGATCGTCCTGGCCTTCTAAACGTAGGACACATTGAAAAAATG
CAGGAGGGTATTGTACATGTGCTCAGACTCCACCTGCAGAGCAACCACCCGGACGATATC
TTTCTCTTCCCAAAACTTCTTCAAAAAATGGCAGACCTCCGGCAGCTGGTGACGGAGCAT
GCGCAGCTGGTGCAGATCATCAAGAAGACGGAGTCGGATGCTGCGCTGCACCCGCTACTG
CAGGAGATCTACAGGGACATGTACTGA
>OriGene 5' read for NM_005036 unedited
NCCCGTTCAAATTTTGTATACGACTCACTAAAGGCGGCCGCGAATATCGCACGAAGAGCA
GATTCAGTGTCATGTGAAGACGTGCTTCCTTGCTTCATAGATAAGAGTGCCACACCCCCC
TGTCCCAGTGCACTGGGGCTGTGGATCCCTTCAAAGCTGAGATTGCCTGTCTGTGGTCTC
CAGCGTTAAGCACAGTCATTAGCTCAGCTTGGAGCTCGGCGGCACAACCAGCACCATCTG
GTCGCGATGGTGGACACGGAAAGCCCACTTCTTGCCCCCTCTCCCCACTCGAGGCCGGCG
ATCTAGAGAGCCCGTTATCTGAAGAGTTCCTGCAAGAAATGGGAAACATCCAAGAGATTT
CGCAATCCATCGGCAAGGATAGTTCTGGAAGCTTTGGCTTTACGGAATACCAGTATTTAG
GAAAGCTGTCCTGGTNCAGATGGGCTCGGTCATCACGGACACGCTTTCACCAGCTTCGAG
CCCTCCCTCGGTGACTATNCTTGTGGTCCCCGGCAGCGTGGACGAGTCTCCCAGTGGAGC
ATTGAACATCGAATGTAGAATCTGCGGGGACAAGGCCTCAGGCTATCATTAACGGAGTCC
ACGCGTGGTGAAGGCTGCAAGGGCCTTCTTTCGGCGAACGATTCGACTCAGCCTGGNNGT
ATGAACAAGTGCGACCGCAGCTGGCNAGATCCAGAAAAAGAACAGAACANATGCCAATTA
TGGGGNCGATTTCACAGTGCCTTTTTTGGGGGGGGGGATGTCACACACCCAAATTTTTTT
TTTGGAACGATGCCCCAAAATTTTGAGAAAGCCAAACTGAAAGCAGAAATTCTTTACCTG
TGACATGAATATTNAAAGATTCTGGACCTTGCAGATCTAAAAATCTCTGCCA
>OriGene 3' read for NM_005036 unedited
ATGGGGTAATTCGTTAAGTACACCGATCNAGNGAANNNGAAGCNANGCGGAAGCANGGNG
ATATAAGATGATGAAAAAAAAACTCTGGTAAAGCAATATACGAATAAGTCTGATATAAGT
GGGACTACTGAGTGTGCCACCGGAGAACTATTTTATAATTTTGTGACAGACAGGGTATTG
CTAGCCGCCTATGATGATTTGAAGGATGGGCTAAGTGATGGGGCCATCTTGGCCTCCAAA
AGTGCTGGGATTACATGTGTGAGCCAGTGTGCCTAGGCTGATTACAAAAACCAAAACGGA
ATAAATTTGTAATTTTTAAAAAAGGATGTTCTAACCATAAGTTTCACTTTTTTCTAGTGG
AAGTTTNACACTGTGTCCTTACAACTCTAATATTTTTATAATGCTGGTCATGGGACCGTT
ATAAATCCTAAAGATTGTTAAAATAATAATGACGTGAGAAGAGACAAATTTTATAAAGAT
GAGGGGAGAGGAATTAAAGATACTTTGAGGTGCAGAGAGATTCCCTGGAGGAGGAGGTCC
GGGGAGTTCCAGAGCAGGATCTAAAAAGGTTTAAGAGGAAAGTTTATATACACAGAGGAC
CAAGAGAGAAAGTGGTATAAGGTGAATAGTTTTCTTGACAAAATAGGATAATAAAGGGTG
ACTTCCCAACTGGGACAGAAGGGGGTAAGTTGGGTTTGAAGGCTAAAAAAAAAGTTCACA
TTTTGTAAAAACTCGCTCACCACCAAGGAGACTAAAAAACCTAAACCACTGAAAAAAAAA
CCCAAACTCGGGAAATGTGAAATATAAAGAAAACAGCCCAGTGAGGAAAATCGGCCCCCC
TCCCCCGCCCCCCGCCCCACGCG
Restriction Sites NotI-NotI     
ACCN NM_005036
Insert Size 4200 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005036.4, NP_005027.2
RefSeq Size 10049 bp
RefSeq ORF 1407 bp
Locus ID 5465
Cytogenetics 22q13.31
Domains HOLI, zf-C4
Protein Families Druggable Genome, Nuclear Hormone Receptor, Transcription Factors
Protein Pathways Adipocytokine signaling pathway, PPAR signaling pathway
Gene Summary 'Peroxisome proliferators include hypolipidemic drugs, herbicides, leukotriene antagonists, and plasticizers; this term arises because they induce an increase in the size and number of peroxisomes. Peroxisomes are subcellular organelles found in plants and animals that contain enzymes for respiration and for cholesterol and lipid metabolism. The action of peroxisome proliferators is thought to be mediated via specific receptors, called PPARs, which belong to the steroid hormone receptor superfamily. PPARs affect the expression of target genes involved in cell proliferation, cell differentiation and in immune and inflammation responses. Three closely related subtypes (alpha, beta/delta, and gamma) have been identified. This gene encodes the subtype PPAR-alpha, which is a nuclear transcription factor. Multiple alternatively spliced transcript variants have been described for this gene, although the full-length nature of only two has been determined. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (5) represents the longer transcript. Variants 3 and 5 encode the same protein.

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