ALK (NM_004304) Human Untagged Clone

CAT#: SC308992

ALK (untagged)-Human anaplastic lymphoma receptor tyrosine kinase (ALK)


  "NM_004304" in other vectors (7)

Reconstitution Protocol

USD 3,290.00

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Size
    • 10 ug

Product Images

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ALK
Synonyms CD246; NBLST3
Vector pCMV6-XL4
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF sequence for NM_004304 edited
GCCCTTCTGTTCAGAGCCTCTTCCCATCTCGGGGAGAGCGAAGGGTGAGGCTGGGCCCGG
AGAGCAGTGTAAACGGCCTCCTCCGGCGGGATGGGAGCCATCGGGCTCCTGTGGCTGCTG
CCGCTGCTGCTTTCCACGGCAGCTGTGGGCTCCGGGATGGGGACCGGCCAGCGCGCGGGC
TCCCCAGCTGCGGGGCCGCCGCTGCAGCCCCGGGAGCCACTCAGCTACTCGCGCCTGCAG
AGGAAGAGTCTGGCAGTTGACTTCGTGGTGCCCTCGCTCTTCCGTGTCTACGCCCGGGAC
CTACTGCTGCCACCATCCTCCTCGGAGCTGAAGGCTGGCAGGCCCGAGGCCCGCGGCTCG
CTAGCTCTGGACTGCGCCCCGCTGCTCAGGTTGCTGGGGCCGGCGCCGGGGGTCTCCTGG
ACCGCCGGTTCACCAGCCCCGGCAGAGGCCCGGACGCTGTCCAGGGTGCTGAAGGGCGGC
TCCGTGCGCAAGCTCCGGCGTGCCAAGCAGTTGGTGCTGGAGCTGGGCGAGGAGGCGATC
TTGGAGGGTTGCGTCGGGCCCCCCGGGGAGGCGGCTGTGGGGCTGCTCCAGTTCAATCTC
AGCGAGCTGTTCAGTTGGTGGATTCGCCAAGGCGAAGGGCGACTGAGGATCCGCCTGATG
CCCGAGAAGAAGGCGTCGGAAGTGGGCAGAGAGGGAAGGCTGTCCGCGGCAATTCGCGCC
TCCCAGCCCCGCCTTCTCTTCCAGATCTTCGGGACTGGTCATAGCTCCTTGGAATCACCA
ACAAACATGCCATCTCCTTCTCCTGATTATTTTACATGGAATCTCACCTGGATAATGAAA
GACTCCTTCCCTTTCCTGTCTCATCGCAGCCGATATGGTCTGGAGTGCAGCTTTGACTTC
CCCTGTGAGCTGGAGTATTCCCCTCCACTGCATGACCTCAGGAACCAGAGCTGGTCCTGG
CGCCGCATCCCCTCCGAGGAGGCCTCCCAGATGGACTTGCTGGATGGGCCTGGGGCAGAG
CGTTCTAAGGAGATGCCCAGAGGCTCCTTTCTCCTTCTCAACACCTCAGCTGACTCCAAG
CACACCATCCTGAGTCCGTGGATGAGGAGCAGCAGTGAGCACTGCACACTGGCCGTCTCG
GTGCACAGGCACCTGCAGCCCTCTGGAAGGTACATTGCCCAGCTGCTGCCCCACAACGAG
GCTGCAAGAGAGATCCTCCTGATGCCCACTCCAGGGAAGCATGGTTGGACAGTGCTCCAG
GGAAGAATCGGGCGTCCAGACAACCCATTTCGAGTGGCCCTGGAATACATCTCCAGTGGA
AACCGCAGCTTGTCTGCAGTGGACTTCTTTGCCCTGAAGAACTGCAGTGAAGGAACATCC
CCAGGCTCCAAGATGGCCCTGCAGAGCTCCTTCACTTGTTGGAATGGGACAGTCCTCCAG
CTTGGGCAGGCCTGTGACTTCCACCAGGACTGTGCCCAGGGAGAAGATGAGAGCCAGATG
TGCCGGAAACTGCCTGTGGGTTTTTACTGCAACTTTGAAGATGGCTTCTGTGGCTGGACC
CAAGGCACACTGTCACCCCACACTCCTCAGTGGCAGGTCAGGACCCTAAAGGATGCCCGG
TTCCAGGACCACCAAGACCATGCTCTATTGCTCAGTACCACTGATGTCCCCGCTTCTGAA
AGTGCTACAGTGACCAGTGCTACGTTTCCTGCACCGATCAAGAGCTCTCCATGTGAGCTC
CGAATGTCCTGGCTCATTCGTGGAGTCTTGAGGGGAAACGTGTCCTTGGTGCTAGTGGAG
AACAAAACCGGGAAGGAGCAAGGCAGGATGGTCTGGCATGTCGCCGCCTATGAAGGCTTG
AGCCTGTGGCAGTGGATGGTGTTGCCTCTCCTCGATGTGTCTGACAGGTTCTGGCTGCAG
ATGGTCGCATGGTGGGGACAAGGATCCAGAGCCATCGTGGCTTTTGACAATATCTCCATC
AGCCTGGACTGCTACCTCACCATTAGCGGAGAGGACAAGATCCTGCAGAATACAGCACCC
AAATCAAGAAACCTGTTTGAGAGAAACCCAAACAAGGAGCTGAAACCCGGGGAAAATTCA
CCAAGACAGACCCCCATCTTTGACCCTACAGTTCATTGGCTGTTCACCACATGTGGGGCC
AGCGGGCCCCATGGCCCCACCCAGGCACAGTGCAACAACGCCTACCAGAACTCCAACCTG
AGCGTGGAGGTGGGGAGCGAGGGCCCCCTGAAAGGCATCCAGATCTGGAAGGTGCCAGCC
ACCGACACCTACAGCATCTCGGGCTACGGAGCTGCTGGCGGGAAAGGCGGGAAGAACACC
ATGATGCGGTCCCACGGCGTGTCTGTGCTGGGCATCTTCAACCTGGAGAAGGATGACATG
CTGTACATCCTGGTTGGGCAGCAGGGAGAGGACGCCTGCCCCAGTACAAACCAGTTAATC
CAGAAAGTCTGCATTGGAGAGAACAATGTGATAGAAGAAGAAATCCGTGTGAACAGAAGC
GTGCATGAGTGGGCAGGAGGCGGAGGAGGAGGGGGTGGAGCCACCTACGTATTTAAGATG
AAGGATGGAGTGCCGGTGCCCCTGATCATTGCAGCCGGAGGTGGTGGCAGGGCCTACGGG
GCCAAGACAGACACGTTCCACCCAGAGAGACTGGAGAATAACTCCTCGGTTCTAGGGCTA
AACGGCAATTCCGGAGCCGCAGGTGGTGGAGGTGGCTGGAATGATAACACTTCCTTGCTC
TGGGCCGGAAAATCTTTGCAGGAGGGTGCCACCGGAGGACATTCCTGCCCCCAGGCCATG
AAGAAGTGGGGGTGGGAGACAAGAGGGGGTTTCGGAGGGGGTGGAGGGGGGTGCTCCTCA
GGTGGAGGAGGCGGAGGATATATAGGCGGCAATGCAGCCTCAAACAATGACCCCGAAATG
GATGGGGAAGATGGGGTTTCCTTCATCAGTCCACTGGGCATCCTGTACACCCCAGCTTTA
AAAGTGATGGAAGGCCACGGGGAAGTGAATATTAAGCATTATCTAAACTGCAGTCACTGT
GAGGTAGACGAATGTCACATGGACCCTGAAAGCCACAAGGTCATCTGCTTCTGTGACCAC
GGGACGGTGCTGGCTGAGGATGGCGTCTCCTGCATTGTGTCACCCACCCCGGAGCCACAC
CTGCCACTCTCGCTGATCCTCTCTGTGGTGACCTCTGCCCTCGTGGCCGCCCTGGTCCTG
GCTTTCTCCGGCATCATGATTGTGTACCGCCGGAAGCACCAGGAGCTGCAAGCCATGCAG
ATGGAGCTGCAGAGCCCTGAGTACAAGCTGAGCAAGCTCCGCACCTCGACCATCATGACC
GACTACAACCCCAACTACTGCTTTGCTGGCAAGACCTCCTCCATCAGTGACCTGAAGGAG
GTGCCGCGGAAAAACATCACCCTCATTCGGGGTCTGGGCCATGGAGCCTTTGGGGAGGTG
TATGAAGGCCAGGTGTCCGGAATGCCCAACGACCCAAGCCCCCTGCAAGTGGCTGTGAAG
ACGCTGCCTGAAGTGTGCTCTGAACAGGACGAACTGGATTTCCTCATGGAAGCCCTGATC
ATCAGCAAATTCAACCACCAGAACATTGTTCGCTGCATTGGGGTGAGCCTGCAATCCCTG
CCCCGGTTCATCCTGCTGGAGCTCATGGCGGGGGGAGACCTCAAGTCCTTCCTCCGAGAG
ACCCGCCCTCGCCCGAGCCAGCCCTCCTCCCTGGCCATGCTGGACCTTCTGCACGTGGCT
CGGGACATTGCCTGTGGCTGTCAGTATTTGGAGGAAAACCACTTCATCCACCGAGACATT
GCTGCCAGAAACTGCCTCTTGACCTGTCCAGGCCCTGGAAGAGTGGCCAAGATTGGAGAC
TTCGGGATGGCCCGAGACATCTACAGGGCGAGCTACTATAGAAAGGGAGGCTGTGCCATG
CTGCCAGTTAAGTGGATGCCCCCAGAGGCCTTCATGGAAGGAATATTCACTTCTAAAACA
GACACATGGTCCTTTGGAGTGCTGCTATGGGAAATCTTTTCTCTTGGATATATGCCATAC
CCCAGCAAAAGCAACCAGGAAGTTCTGGAGTTTGTCACCAGTGGAGGCCGGATGGACCCA
CCCAAGAACTGCCCTGGGCCTGTATACCGGATAATGACTCAGTGCTGGCAACATCAGCCT
GAAGACAGGCCCAACTTTGCCATCATTTTGGAGAGGATTGAATACTGCACCCAGGACCCG
GATGTAATCAACACCGCTTTGCCGATAGAATATGGTCCACTTGTGGAAGAGGAAGAGAAA
GTGCCTGTGAGGCCCAAGGACCCTGAGGGGGTTCCTCCTCTCCTGGTCTCTCAACAGGCA
AAACGGGAGGAGGAGCGCAGCCCAGCTGCCCCACCACCTCTGCCTACCACCTCCTCTGGC
AAGGCTGCAAAGAAACCCACAGCTGCAGAGGTCTCTGTTCGAGTCCCTAGAGGGCCGGCC
GTGGAAGGGGGACACGTGAATATGGCATTCTCTCAGTCCAACCCTCCTTCGGAGTTGCAC
AGGGTCCACGGATCCAGAAACAAGCCCACCAGCTTGTGGAACCCAACGTACGGCTCCTGG
TTTACAGAGAAACCCACCAAAAAGAATAATCCTATAGCAAAGAAGGAGCCACACGAGAGG
GGTAACCTGGGGCTGGAGGGAAGCTGTACTGTCCCACCTAACGTTGCAACTGGGAGACTT
CCGGGGGCCTCACTGCTCCTAGAGCCCTCTTCGCTGACTGCCAATATGAAGGAGGTACCT
CTGTTCAGGCTACGTCACTTCCCTTGTGGGAATGTCAATTACGGCTACCAGCAACAGGGC
TTGCCCTTAGAAGCCGCTACTGCCCCTGGAGCTGGTCATTACGAGGATACCATTCTGAAA
AGCAAGAATAGCATGAACCAGCCTGGGCCCTGAGCTCGGTCGCACACTCACTTCTCTTCC
TTGGGATCCCTAAGACCGTGGAGGAGAGAGAGGCAATCAATGGCTCCTTCACAAACCAGA
GACCAAATGTCACGTTTTGTTTTGTGCCAACCTATTTTGAAGTACCACCAAAAAAGCTGT
ATTTTGAAAATGCTTTAGAAAGGTTTTGAGCATGGGTTCATCCTATTCTTTCGAAAGAAG
AAAATATCATAAAAATGAGTGATAAATACAAGGCCCAGATGTGGTTGCATAAGGTTTTTA
TGCATGTTTGTTGTATACTTCCTTATGCTTCTTTTAAATTGTGTGTGCTCTGCTTCAATG
TAGTCAGAATTAGCTGCTTCTATGTTTCATAGTTGGGGTCATAGATGTTTCCTTGCCTTG
TTGATGTGGACATGAGCCATTTGAGGGGAGAGGGAACGGAAATAAAGGAGTTATTTGTAA
TGACTAAGCATGGGGAAAGACATTCTTTACTTGAAAAAGAAAAAATCATAGACAACTGAA
ATGTCACTTTAGGTGACGGTTAGATGCTTTTAATTGTGCTGATTCATCACCAATTGTAAA
AAATGTCGTGAGTAGTTCCAGTAGTATAGCAGAAGTGTGTATATACTCATCTCAATGAAA
TGCATACA
>OriGene 5' read for NM_004304 unedited
NGACAACATTTGTATACGACTCATATAGGCGGCCGCGNATTCGCCCTTCTGTTCANAGCC
TCTTCCCATCTCGGGAGACGAAGGTGAGGCTGGGCCCGGAGAGCAGTGTAAACGGCCTCC
TCCGGCGGGATGGGAGCCATCGGGCTCCTGTGGCTGCTGCCGCTGCTGCTTTCCACGGCA
GCTGTGGGCTCCGGGATGGGGACCGGCCAGCGCGCGGGCTCCCCAGCTGCGGGGCCGCCG
CTGCAGCCCCGGGAGCCACTCAGCTACTCGCGCCTGCAGAGGAAGAGTCTGGCAGTTGAC
TTCGTGGTGCCCTCGCTCTTCCGTGTCTACGCCCGGGACCTACTGCTGCCACCATCCTCC
TCGGAGCTGAAGGCTGGCAGGCCCGAGGCCCGCGGCTCGCTAGCTCTGGACTGCGCCCCG
CTGCTCAGGTTGCTGGGGCCGGCGCCGGGGGTCTCCTGGACCGCCGGTTCACCAGCCCCG
GCAGAGGCCCGGACGCTGTCCAGGGTGCTGAAGGGCGGCTCCGTGCGCAAGCTCCGGCGT
GCCAAGCAGTTGGTGCTGGAGCTGGGCGAGGAGGCGATCTTGGAGGGTTGCGTCGGGCCC
CCCGGGGAGGCGGCTGTGGGGCTGCTCCAGTTCAATCTCAGCGAGCTGTTCAGTTGGTGG
ATTCGNCCAAGGCGAAGGCGACTGAGGATCCGCCTGATGCCCGAGAAGAAGCGTCNGAAG
TGGGCAGAGAGGGAAGGCTGTCCGCGGCAATTCGCGCCTNCCAGCCCCGCCTTCTCTTCC
AGATCTTCGGGACTGGTCATAGCTCCTTGGAATCACCAAA
>Reverse primer walk for NM_004304 unedited
AGATTACAACAAACTGCATAAAAACCTTATGCAACCACATCTGGGCCTTGTATTTATCAC
TCATTTTTATGATATTTTCTTCTTTCGAAAGAATAGGATGAACCCATGCTCAAAACCTTT
CTAAAGCATTTTCAAAATACAGCTTTTTTGGTGGTACTTCAAAATAGGTTGGCACAAAAC
AAAACGTGACATTTGGTCTCTGGTTTGTGAAGGAGCCATTGATTGCCTCTCTCTCCTCCA
CGGTCTTAGGGATCCCAAGGAAGAGAAGTGAGTGTGCGACCGAGCTCAGGGCCCAGGCTG
GTTCATGCTATTCTTGCTTTTCAGAATGGTATCCTCGTAATGACCAGCTCCAGGGGCAGT
AGCGGCTTCTAAGGGCAAGCCCTGTTGCTGGTAGCCGTAATTGACATTCCCACAAGGGAA
GTGACGTAGCCTGAACAGAGGTACCTCCTTCATATTGGCAGTCAGCGAAGAGGGCTCTAG
GAGCAGTGAGGCCCCCGGAAGTCTCCCAGTTGCAACGTTAGGTGGGACAGTACAGCTTCC
CTCCAGCCCCAGGTTACCCCTCTCGTGTGGCTCCTTCTTTGCTATAGGATTATTCTTTTT
GGTGGGTTTCTCTGTAAACCAGGAGCCGTACGTTGGGTTCCACAAGCTGGTGGGCTTGTT
TCTGGATCCGTGGACCCTGTGCAAACTCCGAAGAGGGTTGGACTGAGAGAATGCCATATT
CACGTGTNCCCCTTCCACGGGCCGGCCCTCTAGGGACTCGAACAGAGACCTCTGCAGCTG
TGGGTTTTCTTTGCAGCCTTGCCAAAAGGAGGTGGTAGGCCAAAAGTGGTGGGGGCAACC
CGGC
Restriction Sites Please inquire     
ACCN NM_004304
Insert Size 8000 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004304.3, NP_004295.2
RefSeq Size 6267 bp
RefSeq ORF 4863 bp
Locus ID 238
Cytogenetics 2p23.2-p23.1
Protein Families Druggable Genome, Protein Kinase
Gene Summary 'This gene encodes a receptor tyrosine kinase, which belongs to the insulin receptor superfamily. This protein comprises an extracellular domain, an hydrophobic stretch corresponding to a single pass transmembrane region, and an intracellular kinase domain. It plays an important role in the development of the brain and exerts its effects on specific neurons in the nervous system. This gene has been found to be rearranged, mutated, or amplified in a series of tumours including anaplastic large cell lymphomas, neuroblastoma, and non-small cell lung cancer. The chromosomal rearrangements are the most common genetic alterations in this gene, which result in creation of multiple fusion genes in tumourigenesis, including ALK (chromosome 2)/EML4 (chromosome 2), ALK/RANBP2 (chromosome 2), ALK/ATIC (chromosome 2), ALK/TFG (chromosome 3), ALK/NPM1 (chromosome 5), ALK/SQSTM1 (chromosome 5), ALK/KIF5B (chromosome 10), ALK/CLTC (chromosome 17), ALK/TPM4 (chromosome 19), and ALK/MSN (chromosome X).[provided by RefSeq, Jan 2011]'
Transcript Variant: This variant (1) encodes the longer isoform (1).

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