IRE1 (ERN1) (NM_001433) Human Untagged Clone

CAT#: SC309043

ERN1 (untagged)-Human endoplasmic reticulum to nucleus signaling 1 (ERN1)


  "NM_001433" in other vectors (6)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol ERN1
Synonyms hIRE1p; IRE1; IRE1a; IRE1P
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene ORF within SC309043 sequence for NM_001433 edited (data generated by NextGen Sequencing)


ATGCCGGCCCGGCGGCTGCTGCTGCTGCTGACGCTGCTGCTGCCCGGCCTCGGGATTTTTGGAAGTACCA
GCACAGTGACGCTTCCTGAAACCTTGTTGTTTGTGTCAACGCTGGATGGAAGTTTGCATGCTGTCAGCAA
GAGGACAGGCTCAATCAAATGGACTTTAAAAGAAGATCCAGTCCTGCAGGTCCCAACACATGTGGAAGAG
CCTGCCTTTCTCCCAGATCCTAATGATGGCAGCCTGTATACGCTTGGAAGCAAGAATAATGAAGGCCTGA
CGAAACTTCCTTTTACCATCCCAGAATTGGTGCAGGCATCCCCATGCCGAAGTTCAGATGGAATCCTCTA
CATGGGTAAAAAGCAGGACATCTGGTATGTTATTGACCTCCTGACCGGAGAGAAGCAGCAGACTTTGTCA
TCGGCCTTTGCAGATAGTCTCTGCCCATCAACCTCTCTTCTGTATCTTGGGCGAACAGAATACACCATCA
CCATGTACGACACCAAAACCCGAGAGCTCCGGTGGAATGCCACCTACTTTGACTATGCGGCCTCACTGCC
TGAGGACGACGTGGACTACAAGATGTCCCACTTTGTGTCCAATGGTGATGGGCTGGTGGTGACTGTGGAC
AGTGAATCTGGGGACGTCCTATGGATCCAAAACTACGCCTCCCCTGTGGTGGCCTTTTATGTCTGGCAGC
GGGAGGGTCTGAGGAAGGTGATGCACATCAATGTCGCTGTGGAGACCCTGCGCTATCTGACCTTCATGTC
TGGGGAGGTGGGGCGCATCACAAAGTGGAAGTACCCGTTCCCCAAGGAGACAGAGGCCAAGAGCAAGCTG
ACGCCCACTCTGTATGTTGGGAAATACTCTACCAGCCTCTATGCCTCTCCCTCAATGGTACACGAGGGGG
TTGCTGTCGTGCCCCGCGGCAGCACACTTCCTTTGCTGGAAGGGCCCCAGACTGATGGCGTCACCATCGG
GGACAAGGGGGAGTGTGTGATCACGCCCAGCACGGACGTCAAGTTTGATCCCGGACTCAAAAGCAAGAAC
AAGCTCAACTACTTGAGGAATTACTGGCTTCTGATAGGACACCATGAAACCCCACTGTCTGCGTCTACCA
AGATGCTGGAGAGATTTCCCAACAATCTACCCAAACATCGGGAAAATGTGATTCCTGCTGATTCAGAGAA
AAAGAGCTTTGAGGAAGTTATCAACCTGGTTGACCAGACTTCAGAAAACGCACCTACCACCGTGTCTCGG
GATGTGGAGGAGAAGCCCGCCCATGCCCCTGCCCGGCCCGAGGCCCCCGTGGACTCCATGCTTAAGGACA
TGGCTACCATCATCCTGAGCACCTTCCTGCTGATTGGCTGGGTGGCCTTCATCATCACCTATCCCCTGAG
CATGCATCAGCAGCAGCAGCTCCAGCACCAGCAGTTCCAGAAGGAACTGGAGAAGATCCAGCTCCTGCAG
CAGCAGCAGCAGCAGCTGCCCTTCCACCCACCTGGAGACACGGCTCAGGACGGCGAGCTCCTGGACACGT
CTGGCCCGTACTCAGAGAGCTCGGGCACCAGCAGCCCCAGCACGTCCCCCAGGGCCTCCAACCACTCGCT
CTGCTCCGGCAGCTCTGCCTCCAAGGCTGGCAGCAGCCCCTCCCTGGAACAAGACGATGGAGATGAGGAA
ACCAGCGTGGTGATAGTTGGGAAAATTTCCTTCTGTCCCAAGGATGTCCTGGGCCATGGAGCTGAGGGCA
CAATTGTGTACCGGGGCATGTTTGACAACCGCGACGTGGCCGTGAAGAGGATCCTCCCCGAGTGTTTTAG
CTTCGCAGACCGTGAGGTCCAGCTGTTGCGAGAATCGGATGAGCACCCGAACGTGATCCGCTACTTCTGC
ACGGAGAAGGACCGGCAATTCCAGTACATTGCCATCGAGCTGTGTGCAGCCACCCTGCAAGAGTATGTGG
AGCAGAAGGACTTTGCGCATCTCGGCCTGGAGCCCATCACCTTGCTGCAGCAGACCACCTCGGGCCTGGC
CCACCTCCACTCCCTCAACATCGTTCACAGAGACCTAAAGCCACACAACATCCTCATATCCATGCCCAAT
GCACACGGCAAGATCAAGGCCATGATCTCCGACTTTGGCCTCTGCAAGAAGCTGGCAGTGGGCAGACACA
GTTTCAGCCGCCGATCTGGGGTGCCTGGCACAGAAGGCTGGATCGCTCCAGAGATGCTGAGCGAAGACTG
TAAGGAGAACCCTACCTACACGGTGGACATCTTTTCTGCAGGCTGCGTCTTTTACTACGTAATCTCTGAG
GGCAGCCACCCTTTTGGCAAGTCCCTGCAGCGGCAGGCCAACATCCTCCTGGGTGCCTGCAGCCTTGACT
GCTTGCACCCAGAGAAGCACGAAGACGTCATTGCACGTGAATTGATAGAGAAGATGATTGCGATGGATCC
TCAGAAACGCCCCTCAGCGAAGCACGTGCTCAAACACCCGTTCTTCTGGAGCCTAGAGAAGCAGCTCCAG
TTCTTCCAGGACGTGAGCGACAGAATAGAAAAGGAATCCCTGGATGGCCCGATCGTGAAGCAGTTAGAGA
GAGGCGGGAGAGCCGTGGTGAAGATGGACTGGCGGGAGAACATCACTGTCCCCCTCCAGACAGACCTGCG
TAAATTCAGGACCTATAAAGGTGGTTCTGTCAGAGATCTCCTCCGAGCCATGAGAAATAAGAAGCACCAC
TACCGGGAGCTGCCTGCAGAGGTGCGGGAGACGCTGGGGTCCCTCCCCGACGACTTCGTGTGCTACTTCA
CATCTCGCTTCCCCCACCTCCTCGCACACACCTACCGGGCCATGGAGCTGTGCAGCCACGAGAGACTCTT
CCAGCCCTACTACTTCCACGAGCCCCCAGAGCCCCAGCCCCCAGTGACTCCAGACGCCCTCTGA


Clone variation with respect to NM_001433.4
651:a=>g 978:c=>t 2475:c=>t
>OriGene 5' read for NM_001433 unedited
GAGGTCATTATTGTATACGACTCACTATAGGCGGCCGCGAATCGCCCCGTCCTCTGTCCG
TACCGCCCCCGGAGCCAGGGCCGAGTCCTCGCCATGCCGGCCCGGCGGCTGCTGCTGCTG
CTGACGCTGCTGCTGCCCGGCCTCGGGATTTTTGGAAGTACCAGCACAGTGACGCTTCCT
GAAACCTTGTTGTTTGTGTCAACGCTGGATGGAAGTTTGCATGCTGTCAGCAAGAGGACA
GGCTCAATCAAATGGACTTTAAAAGAAGATCCAGTCCTGCAGGTCCCAACACATGTGGAA
GAGCCTGCCTTTCTCCCAGATCCTAATGATGGCAGCCTGTATACGCTTGGAAGCAAGAAT
AATGAAGGCCTGACGAAACTTCCTTTTACCATCCCAGAATTGGTGCAGGCATCCCCATGC
CGAAGTTCAGATGGAATCCTCTACATGGGTAAAAAGCAGGACATCTGGTATGTTATTGAC
CTCCTGACCGGAGAGAAGCAGCAGACTTTGTCATCGGCCTTTGCAGATAGTCTCTGCCCA
TCAACCTCTCTTCTGTATCTTGNGCGAACAGAATACACCATCACCATGTACGACACCAAA
ACCCGAGAGCTCCGGTGGAATGCCACCTACTTTGACTATGCGGCCTCACTGCCTGAGGAC
GACGTGGACTACAAGATGTCCCACTTTGTGTCCAATGGTGATGGGCTGGTGGTGACTGTG
GACAGTGAATCTGGGGACGTCCTATGGATCCCAAACTACGCCTCCCCTGTGGTGGCCTTT
TATGTCTGGCACCGGGAGGGTCTGAAGAAAGTGATGCACATCAATGTCGCTGTGGAGACC
CTGCGCTATCTGACCTTTATGTCTGGGGGAGGGTGGGGCGCATCACAAAGTGG
Restriction Sites Please inquire     
ACCN NM_001433
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001433.2, NP_001424.2
RefSeq Size 7910 bp
RefSeq ORF 2934 bp
Locus ID 2081
Cytogenetics 17q23.3
Domains pkinase, TyrKc, S_TKc, PQQ, PUG
Protein Families Protein Kinase, Transmembrane
Protein Pathways Alzheimer's disease
Gene Summary 'This gene encodes the transmembrane protein kinase inositol-requiring enzyme 1. The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1. [provided by RefSeq, Aug 2017]'
Transcript Variant: This variant (1) consists of multiple exons and encodes the longer isoform (1).

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