CACNA1E (NM_000721) Human Untagged Clone

CAT#: SC309052

CACNA1E (untagged)-Human calcium channel, voltage-dependent, R type, alpha 1E subunit (CACNA1E), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CACNA1E
• Synonyms: BII; CACH6; CACNL1A6; Cav2.3; EIEE69; gm139
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000721, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCTCGCTTCGGGGAGGCGGTGGTCGCCAGGCCAGGGTCCGGCGATGGAGACTCGGACCAGAGCAGGA
  ACCGGCAAGGAACCCCCGTGCCGGCCTCGGGGCAGGCGGCCGCCTACAAGCAGACGAAAGCACAGAGGGC
  GCGGACTATGGCTTTGTACAACCCCATTCCCGTCCGGCAGAACTGTTTCACCGTCAACAGATCCCTGTTC
  ATCTTCGGAGAAGATAACATTGTCAGGAAATATGCCAAGAAGCTCATCGATTGGCCGCCATTTGAGTACA
  TGATCCTGGCCACCATCATTGCCAACTGCATCGTCCTGGCCCTGGAGCAGCATCTTCCTGAGGATGACAA
  GACCCCCATGTCCCGAAGACTGGAGAAGACAGAACCTTATTTCATTGGGATCTTTTGCTTTGAAGCTGGG
  ATCAAAATTGTGGCCCTGGGGTTCATCTTCCATAAGGGCTCTTACCTCCGCAATGGCTGGAATGTCATGG
  ACTTCATCGTGGTCCTCAGTGGCATCCTGGCCACTGCAGGAACCCACTTCAATACTCACGTGGACCTGAG
  GACCCTCCGGGCTGTGCGTGTCCTGCGGCCTTTGAAGCTCGTGTCAGGGATACCTAGCCTGCAGATTGTG
  TTGAAGTCCATCATGAAGGCCATGGTACCTCTTCTGCAGATTGGCCTTCTGCTCTTCTTTGCCATCCTGA
  TGTTTGCTATCATTGGTTTGGAGTTCTACAGTGGCAAGTTACATCGAGCATGCTTCATGAACAATTCAGG
  TATTCTAGAAGGATTTGACCCCCCTCACCCATGTGGTGTGCAGGGCTGCCCAGCTGGTTATGAATGCAAG
  GACTGGATCGGCCCCAATGATGGGATCACCCAGTTTGATAACATCCTTTTTGCTGTGCTGACTGTCTTCC
  AGTGCATCACCATGGAAGGGTGGACCACTGTGCTGTACAATACCAATGATGCCTTAGGAGCCACCTGGAA
  TTGGCTGTACTTCATCCCCCTCATCATCATTGGATCCTTCTTTGTTCTCAACCTAGTCCTGGGAGTGCTT
  TCCGGGGAATTTGCCAAAGAGAGAGAGAGAGTGGAGAACCGAAGGGCTTTCATGAAGCTGCGGCGCCAGC
  AGCAGATTGAGCGTGAGCTGAATGGCTACCGTGCCTGGATAGACAAAGCAGAGGAAGTCATGCTCGCTGA
  AGAAAATAAAAATGCTGGAACATCCGCCTTAGAAGTGCTTCGAAGGGCAACCATCAAGAGGAGCCGGACA
  GAGGCCATGACTCGAGACTCCAGTGATGAGCACTGTGTTGATATCTCCTCTGTGGGCACACCTCTGGCCC
  GAGCCAGTATCAAAAGTGCAAAGGTAGACGGGGTCTCTTATTTCCGGCACAAGGAAAGGCTTCTGCGCAT
  CTCCATTCGCCACATGGTTAAATCCCAGGTGTTTTACTGGATTGTGCTGAGCCTTGTGGCACTCAACACT
  GCCTGTGTGGCCATTGTCCATCACAACCAGCCCCAGTGGCTCACCCACCTCCTCTACTATGCAGAATTTC
  TGTTTCTGGGACTCTTCCTCTTGGAGATGTCCCTGAAGATGTATGGCATGGGGCCTCGCCTTTATTTTCA
  CTCTTCATTCAACTGCTTTGATTTTGGGGTCACAGTGGGCAGTATCTTTGAAGTGGTCTGGGCAATCTTC
  AGACCTGGTACGTCTTTTGGAATCAGTGTCTTGCGAGCCCTCCGGCTTCTAAGAATATTTAAAATAACCA
  AGTATTGGGCTTCCCTACGGAATTTGGTGGTCTCCTTGATGAGCTCAATGAAGTCTATCATCAGTTTGCT
  TTTCCTCCTCTTCCTCTTCATCGTTGTCTTTGCTCTCCTAGGAATGCAGTTATTTGGAGGCAGGTTTAAC
  TTTAATGATGGGACTCCTTCGGCAAATTTTGATACCTTCCCTGCAGCCATCATGACTGTGTTCCAGATCC
  TGACGGGTGAGGACTGGAATGAGGTGATGTACAATGGGATCCGCTCCCAGGGTGGGGTCAGCTCAGGCAT
  GTGGTCTGCCATCTACTTCATTGTGCTCACCTTGTTTGGCAACTACACGCTACTGAATGTGTTCTTGGCT
  ATCGCTGTGGATAATCTCGCCAACGCCCAGGAACTGACCAAGGATGAACAGGAGGAAGAAGAGGCCTTCA
  ACCAGAAACATGCACTGCAGAAGGCCAAGGAGGTCAGCCCGATGTCTGCACCCAACATGCCTTCGATCGA
  AAGAGACAGAAGGAGAAGACACCACATGTCGATGTGGGAGCCACGCAGCAGCCACCTGAGGGAGCGGAGG
  CGCCGGCACCACATGTCCGTGTGGGAGCAGCGTACCAGCCAGCTGAGGAAGCACATGCAGATGTCCAGCC
  AGGAGGCCCTCAACAGAGAGGAGGCGCCGACCATGAACCCGCTCAACCCCCTCAACCCGCTCAGCTCCCT
  CAACCCGCTCAATGCCCACCCCAGCCTTTATCGGCGACCCAGGGCCATTGAGGGCCTGGCCCTGGGCCTG
  GCCCTGGAGAAGTTCGAGGAGGAGCGCATCAGCCGTGGGGGGTCCCTCAAGGGGGATGGAGGGGACCGAT
  CCAGTGCCCTGGACAACCAGAGGACCCCTTTGTCCCTGGGCCAGCGGGAGCCACCATGGCTGGCCAGGCC
  CTGTCATGGAAACTGTGACCCGACTCAGCAGGAGGCAGGGGGAGGAGAGGCTGTGGTGACCTTTGAGGAC
  CGGGCCAGGCACAGGCAGAGCCAACGGCGCAGCCGGCATCGCCGCGTCAGGACAGAAGGCAAGGAGTCCT
  CTTCAGCCTCCCGGAGCAGGTCTGCCAGCCAGGAACGCAGTCTGGATGAAGCCATGCCCACTGAAGGGGA
  GAAGGACCATGAGCTCAGGGGCAACCATGGTGCCAAGGAGCCAACGATCCAAGAAGAGAGAGCCCAGGAT
  TTAAGGAGGACCAACAGTCTGATGGTGTCCAGAGGCTCCGGGCTGGCAGGAGGCCTTGATGAGGCTGACA
  CCCCCCTAGTCCTGCCCCATCCTGAGCTGGAAGTGGGGAAGCACGTGGTGCTGACGGAGCAGGAGCCAGA
  AGGCAGCAGTGAGCAGGCCCTGCTGGGGAATGTGCAGCTAGACATGGGCCGGGTCATCAGCCAGAGCGAG
  CCTGACCTCTCCTGCATCACGGCCAACACGGACAAGGCCACCACCGAGAGCACCAGCGTCACCGTCGCCA
  TCCCCGACGTGGACCCCTTGGTGGACTCAACCGTGGTGCACATTAGCAACAAGACGGATGGGGAAGCCAG
  TCCCTTGAAGGAGGCAGAGATCAGAGAGGATGAGGAGGAGGTGGAGAAGAAGAAGCAGAAGAAGGAGAAG
  CGTGAGACAGGCAAAGCCATGGTGCCCCACAGCTCAATGTTCATCTTCAGCACCACCAACCCGATCCGGA
  GGGCCTGCCACTACATCGTGAACCTGCGCTACTTTGAGATGTGCATCCTCCTGGTGATTGCAGCCAGCAG
  CATCGCCCTGGCGGCAGAGGACCCCGTCCTGACCAACTCGGAGCGCAACAAAGTCCTGAGGTATTTTGAC
  TATGTGTTCACGGGCGTGTTCACCTTTGAGATGGTTATAAAGATGATAGACCAAGGCTTGATCCTGCAGG
  ATGGGTCCTACTTCCGAGACTTGTGGAACATCCTGGACTTTGTGGTGGTCGTTGGCGCATTGGTGGCCTT
  TGCTCTGGCGAACGCTTTGGGAACCAACAAAGGACGGGACATCAAGACCATCAAGTCTCTGCGGGTGCTC
  CGAGTTCTAAGGCCACTGAAAACCATCAAGCGCTTGCCCAAGCTCAAGGCCGTCTTCGACTGCGTAGTGA
  CCTCCTTGAAGAATGTCTTCAACATACTCATTGTGTACAAGCTCTTCATGTTCATCTTTGCTGTCATCGC
  AGTTCAGCTCTTCAAGGGAAAGTTCTTTTATTGCACGGACAGTTCCAAGGACACAGAGAAGGAGTGCATA
  GGCAACTATGTAGATCATGAGAAAAACAAGATGGAGGTGAAGGGCCGGGAATGGAAGCGCCATGAATTCC
  ACTACGACAACATTATCTGGGCCCTGCTGACCCTCTTCACCGTCTCCACAGGGGAAGGATGGCCTCAAGT
  TCTGCAGCACTCTGTAGATGTGACAGAGGAAGACCGAGGCCCAAGCCGCAGCAACCGCATGGAGATGTCT
  ATCTTTTATGTAGTCTACTTTGTGGTCTTCCCCTTCTTCTTTGTCAATATCTTTGTGGCTCTCATCATCA
  TCACCTTCCAGGAGCAAGGGGATAAGATGATGGAGGAGTGCAGCCTGGAGAAGAATGAGAGGGCGTGCAT
  CGACTTCGCCATCAGCGCCAAACCTCTCACCCGCTACATGCCGCAGAACAGACACACCTTCCAGTACCGC
  GTGTGGCACTTTGTGGTGTCTCCGTCCTTTGAGTACACCATTATGGCCATGATCGCCTTGAATACTGTTG
  TGCTGATGATGAAGTATTATTCTGCTCCCTGTACCTATGAGCTGGCCCTGAAGTACCTGAATATCGCCTT
  CACCATGGTGTTTTCCCTGGAATGTGTCCTGAAGGTCATCGCTTTTGGCTTTTTGAACTATTTCCGAGAC
  ACCTGGAATATCTTTGACTTCATCACCGTGATTGGCAGTATCACAGAAATTATCCTGACAGACAGCAAGC
  TGGTGAACACCAGTGGCTTCAATATGAGCTTTCTGAAGCTCTTCCGAGCTGCCCGCCTCATAAAGCTCCT
  GCGTCAGGGCTATACCATACGCATTTTGCTGTGGACCTTTGTGCAGTCCTTTAAGGCCCTCCCTTATGTC
  TGCCTTTTAATTGCCATGCTTTTCTTCATTTATGCCATCATTGGGATGCAGGTATTTGGAAACATAAAAT
  TAGACGAGGAGAGTCACATCAACCGGCACAACAACTTCCGGAGTTTCTTTGGGTCCCTAATGCTACTCTT
  CAGGAGTGCCACAGGTGAGGCCTGGCAGGAGATTATGCTGTCATGCCTTGGGGAGAAGGGCTGTGAGCCT
  GACACCACCGCACCATCAGGGCAGAACGAGAACGAACGCTGCGGCACCGATCTGGCCTACGTGTACTTTG
  TCTCCTTCATCTTCTTCTGCTCCTTCTTGATGCTCAACCTGTTTGTGGCCGTCATCATGGACAACTTTGA
  GTACCTGACTCGGGACTCCTCCATCCTGGGGCCTCACCACTTGGACGAGTTTGTCCGCGTCTGGGCAGAA
  TATGACCGAGCAGCATGTGGCCGCATCCATTACACTGAGATGTATGAAATGCTGACTCTCATGTCACCTC
  CGCTAGGCCTCGGCAAGAGATGTCCCTCCAAAGTGGCATATAAGAGGTTGGTCCTGATGAACATGCCAGT
  AGCTGAGGACATGACGGTCCACTTCACCTCCACACTTATGGCTCTGATCCGGACAGCTCTGGACATTAAA
  ATTGCCAAAGGTGGTGCAGACAGGCAGCAGCTAGACTCAGAGCTACAAAAGGAGACCCTAGCCATCTGGC
  CTCACCTATCCCAGAAGATGCTGGATCTGCTTGTGCCCATGCCCAAAGCCTCTGACCTGACTGTGGGCAA
  AATCTATGCAGCAATGATGATCATGGACTACTATAAGCAGAGTAAGGTGAAGAAGCAGAGGCAGCAGCTG
  GAGGAACAGAAAAATGCCCCCATGTTCCAGCGCATGGAGCCTTCATCTCTGCCTCAGGAGATCATTGCTA
  ATGCCAAAGCCCTGCCTTACCTCCAGCAGGACCCCGTTTCAGGCCTGAGTGGCCGGAGTGGATACCCTTC
  GATGAGTCCACTCTCTCCCCAGGATATATTCCAGTTGGCTTGTATGGACCCCGCCGATGACGGACAGTTC
  CAAGAACGGCAGTCTCTGGTGGTGACAGACCCTAGCTCCATGAGACGTTCATTTTCCACTATTCGGGATA
  AGCGTTCAAATTCCTCGTGGTTGGAGGAATTCTCCATGGAGCGAAGCAGTGAAAATACCTACAAGTCCCG
  TCGCCGGAGTTACCACTCCTCCTTGCGGCTGTCAGCCCACCGCCTGAACTCTGATTCAGGCCACAAGTCT
  GACACTCACCGCTCAGGGGGCAGGGAGCGGGGACGATCAAAAGAGCGAAAGCATCTTCTCTCTCCTGATG
  TCTCCCGCTGCAATTCAGAAGAGCGAGGGACCCAGGCTGACTGGGAGTCCCCAGAGCGCCGTCAATCCAG
  GTCACCCAGTGAGGGCAGGTCACAGACGCCCAACAGACAGGGCACAGGTTCCCTAAGTGAGAGCTCCATC
  CCCTCTGTCTCTGACACCAGCACCCCAAGAAGAAGTCGTCGGCAGCTCCCACCCGTCCCGCCAAAGCCCC
  GGCCCCTCCTTTCCTACAGCTCCCTGATTCGACACGCGGGCAGCATCTCTCCACCTGCTGATGGAAGCGA
  GGAGGGCTCCCCGCTGACCTCCCAAGCTCTGGAGAGCAACAATGCTTGCCTGACCGAGTCTTCCAACTCT
  CCGCACCCCCAGCAGAGCCAACATGCCTCCCCACAGCGCTACATCTCCGAGCCCTACTTGGCCCTGCACG
  AAGACTCCCACGCCTCAGACTGTGGTGAGGAGGAGACGCTCACTTTCGAAGCAGCCGTGGCTACTAGCCT
  GGGCCGTTCCAACACCATCGGCTCAGCCCCACCCCTGCGGCATAGCTGGCAGATGCCCAACGGGCACTAT
  CGGCGGCGGAGGCGCGGGGGGCCTGGGCCAGGCATGATGTGTGGGGCTGTCAACAACCTGCTAAGTGACA
  CGGAAGAAGATGACAAATGCTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000721
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000721.3, NP_000712.2
• RefSeq Size: 14972 bp
• RefSeq ORF: 6813 bp
• Locus ID: 777
• Cytogenetics: 1q25.3
• Domains: ion_trans
• Protein Families: Druggable Genome, Ion Channels: Calcium, Transmembrane
• Protein Pathways: Calcium signaling pathway, MAPK signaling pathway, Type II diabetes mellitus
• Gene Summary: 'Voltage-dependent calcium channels are multisubunit complexes consisting of alpha-1, alpha-2, beta, and delta subunits in a 1:1:1:1 ratio. These channels mediate the entry of calcium ions into excitable cells, and are also involved in a variety of calcium-dependent processes, including muscle contraction, hormone or neurotransmitter release, gene expression, cell motility, cell division and cell death. This gene encodes the alpha-1E subunit of the R-type calcium channels, which belong to the 'high-voltage activated' group that maybe involved in the modulation of firing patterns of neurons important for information processing. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Apr 2011]'
  Transcript Variant: This variant (3) is missing an in-frame coding exon compared to variant 1. This results in a shorter isoform (3, also known as alpha-1E-3) missing an internal protein segment compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.