UBE3A (NM_000462) Human Untagged Clone

CAT#: SC309057

UBE3A (untagged)-Human ubiquitin protein ligase E3A (UBE3A), transcript variant 2


  "NM_000462" in other vectors (6)

Reconstitution Protocol

USD 1,780.00

In Stock*

Size
    • 10 ug

Product Images

Other products for "UBE3A"

Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol UBE3A
Synonyms ANCR; AS; E6-AP; EPVE6AP; HPVE6A
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_000462 edited
ACCAGCTCCTCAGAAGTTTGGCGAAATATGAGTTATTAAGCCTACGCTCAGATCAAGGTA
GCAGCTAGACTGGTGTGACAACCTGTTTTTAATCAGTGACTCAAAGCTGTGATCACCCTG
ATGTCACCGAATGGCCACAGCTTGTAAAAGAGAGTTACAGTGGAGGTAAAAGGAGTGGCT
TGCAGGATGGAGAAGCTGCACCAGTGTTATTGGAAATCAGGAGAACCTCAGTCTGACGAC
ATTGAAGCTAGCCGAATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCAC
CAGTTAACTGAGGGCTGTGGAAATGAAGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCA
ACTTTTCTTCGTATGGATAATAATGCAGCAGCTATTAAAGCCCTCGAGCTTTATAAGATT
AATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCTTACCTTGAG
AACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAAGAAAGGCGCT
AGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGAAGGTATATGAAATTCTTGAA
TTATGTAGAGAAAGAGAGGATTATTCCCCTTTAATCCGTGTTATTGGAAGAGTTTTTTCT
AGTGCTGAGGCATTGGTACAGAGCTTCCGGAAAGTTAAACAACACACCAAGGAAGAACTG
AAATCTCTTCAAGCAAAAGATGAAGACAAAGATGAAGATGAAAAGGAAAAAGCTGCATGT
TCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCCTCAAGGATAGGTGATAGCTCA
CAGGGAGACAACAATTTGCAAAAATTAGGCCCTGATGATGTGTCTGTGGATATTGATGCC
ATTAGAAGGGTCTACACCAGATTGCTCTCTAATGAAAAAATTGAAACTGCCTTTCTCAAT
GCACTTGTATATTTGTCACCTAACGTGGAATGTGACTTGACGTATCACAATGTATACTCT
CGAGATCCTAATTATCTGAATTTGTTCATTATCGTAATGGAGAATAGAAATCTCCACAGT
CCTGAATATCTGGAAATGGCTTTGCCATTATTTTGCAAAGCGATGAGCAAGCTACCCCTT
GCAGCCCAAGGAAAACTGATCAGACTGTGGTCTAAATACAATGCAGACCAGATTCGGAGA
ATGATGGAGACATTTCAGCAACTTATTACTTATAAAGTCATAAGCAATGAATTTAACAGT
CGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCGAAGTGCTTGAAAATGGTT
TACTATGCAAATGTAGTGGGAGGGGAAGTGGACACAAATCACAATGAAGAAGATGATGAA
GAGCCCATCCCTGAGTCCAGCGAGCTGACACTTCAGGAACTTTTGGGAGAAGAAAGAAGA
AACAAGAAAGGTCCTCGAGTGGACCCCCTGGAAACTGAACTTGGTGTTAAAACCCTGGAT
TGTCGAAAACCACTTATCCCTTTTGAAGAGTTTATTAATGAACCACTGAATGAGGTTCTA
GAAATGGATAAAGATTATACTTTTTTCAAAGTAGAAACAGAGAACAAATTCTCTTTTATG
ACATGTCCCTTTATATTGAATGCTGTCACAAAGAATTTGGGATTATATTATGACAATAGA
ATTCGCATGTACAGTGAACGAAGAATCACTGTTCTCTACAGCTTAGTTCAAGGACAGCAG
TTGAATCCATATTTGAGACTCAAAGTTAGACGTGACCATATCATAGATGATGCACTTGTC
CGGCTAGAGATGATCGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAA
TTTGAAGGAGAACAAGGAGTTGATGAGGGAGGTGTTTCCAAAGAATTTTTTCAGCTGGTT
GTGGAGGAAATCTTCAATCCAGATATTGGTATGTTCACATACGATGAATCTACAAAATTG
TTTTGGTTTAATCCATCTTCTTTTGAAACTGAGGGTCAGTTTACTCTGATTGGCATAGTA
CTGGGTCTGGCTATTTACAATAACTGTATACTGGATGTACATTTTCCCATGGTTGTCTAC
AGGAAGCTAATGGGGAAAAAAGGAACTTTTCGTGACTTGGGAGACTCTCACCCAGTTCTA
TATCAGAGTTTAAAAGATTTATTGGAGTATGAAGGGAATGTGGAAGATGACATGATGATC
ACTTTCCAGATATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAGGAA
AATGGTGATAAAATTCCAATTACAAATGAAAACAGGAAGGAATTTGTCAATCTTTATTCT
GACTACATTCTCAATAAATCAGTAGAAAAACAGTTCAAGGCTTTTCGGAGAGGTTTTCAT
ATGGTGACCAATGAATCTCCCTTAAAGTACTTATTCAGACCAGAAGAAATTGAATTGCTT
ATATGTGGAAGCCGGAATCTAGATTTCCAAGCACTAGAAGAAACTACAGAATATGACGGT
GGCTATACCAGGGACTCTGTTCTGATTAGGGAGTTCTGGGAAATCGTTCATTCATTTACA
GATGAACAGAAAAGACTCTTCTTGCAGTTTACAACGGGCACAGACAGAGCACCTGTGGGA
GGACTAGGAAAATTAAAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCT
ACATCTCATACTTGCTTTAATGTGCTTTTACTTCCGGAATACTCAAGCAAAGAAAAACTT
AAAGAGAGATTGTTGAAGGCCATCACGTATGCCAAAGGATTTGGCATGCTGTAAAACAAA
ACAAAACAAAATAAAACAAAAAAAAGGAAAAAAAAAA
>OriGene 5' read for NM_000462 unedited
GTCCAGTGCATATTTGTATACGACTCATATAGGCGGCCGCGTAATTCGCACGAGGCTGAT
TTTAGAATTCACCAGCTCCTCAGAATTTGGCGAAATATGAGTTATTAAGCCTACGCTCAG
ATCAAGGTAGCAGCTAGACTGGTGTGACAACCTGTTTTTAATCAGTGACTCAAAGCTGTG
ATCACCCTGATGTCACCGAATGGCCACAGCTTGTAAAAGAGAGTTACAGTGGAGGTAAAA
GGAGTGGCTTGCAGGATGGAGAAGCTGCACCAGTGTTATTGGAAATCAGGAGAACCTCAG
TCTGACGACATTGAAGCTAGCCGAATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGC
TACTACCACCAGTTAACTGAGGGCTGTGGAAATGAAGCCTGCACGAATGAGTTTTGTGCT
TCCTGTCCAACTTTTCTTCGTATGGATAATAATGCAGCAGCTATTAAAGCCCTCGAGCTT
TATAAGATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGCTCAGCT
TACCTTGAGAACTCGAAAGGTGCCCCCAACAACTCCTGCTCTGAGATAAAAATGAACAAG
ACAGGCGCTAGAATTGATTTTAAAGATGTGACTTACTTAACAGAAGAGAAGGTATATGAA
ATTCTTGAATTATGTAGAGAAAGAGAGGATTATTCCCCTTTAATCCGTGTTATTGGAAGA
GTTTTTTCTAGTGCTGAGGCATTGGTACAGAGCTTCCGNGAAGTTAAACAACACACCAAG
GGAAGAACTGANNATCTCTTCAGCCANAGATGAAGACCAAGATGAAGATGAANAGGANAA
AGCTGCNTGTTCTGCTGCTGCTATGGAAAGAGACTCANAAGCATCTTCTCA
>Reverse primer walk for NM_000462 unedited
CCTGGNCCAATATGGCTGAAACCCCGTCTCTACCAAAAAAACAAAATTAGCTGAGCCTGG
TCATGCATGCCTGNGAATCCCAACAACTCGGGAGGCTGAGGCAGGAGAATCGCTTGAACC
CAGGAGGCGGAGATTGCAGTGAGCCAAGATTGTGCCACTGCACTCCAGCTTGGTTCCCAA
TAGACCCCGCAGGCCCTACAGGTTGTCTTCCCAACTTGCCCCTTGCTCCATACCACCCCC
CTCCACCCCATAATATTATAGAAGGACACCTAGTCAGACAAAATGATGCAACTTAATTTT
ATTAGGACAAGGCTGGTGGGCACTGGAGTGGCAACTTCCAGGGCCAGGAGAGGCACTGGG
GAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCA
ATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGGTTTATTTTGTTTTGGTTTGTTTTACAG
CATGCCAAATCCTTTGGCATACGTGATGGCCTTCAACAATCTCTCTTTAAATTTTTCTTT
GCTTGAGTCTTCCCGGAAGTAAAAGCCCATTAAAGCAAGTATGAGATGTAAGTAACCTTT
CTGTGTCTGGGCCATTTTTGGGTATAATCATCTTTAAATTTCCCAGACCTCCCAAGGTGC
TCTGGCTGTGCCCGTTGTAAACTGAAAAAGAATCCTTTTTGTTCATCTGTAAATAAAGGA
ACAATTTCCCAAAACTCCTTAACCAAACAAAACCCTGGGTTAGCCCCCCGCCCAATTCTG
GAATTTCTTCCCAAGCCTTGGAAATCAAAATCCCG
Restriction Sites Please inquire     
ACCN NM_000462
Insert Size 2900 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000462.2, NP_000453.2
RefSeq Size 4516 bp
RefSeq ORF 2628 bp
Locus ID 7337
Cytogenetics 15q11.2
Domains HECT
Protein Families Druggable Genome
Protein Pathways Ubiquitin mediated proteolysis
Gene Summary 'This gene encodes an E3 ubiquitin-protein ligase, part of the ubiquitin protein degradation system. This imprinted gene is maternally expressed in brain and biallelically expressed in other tissues. Maternally inherited deletion of this gene causes Angelman Syndrome, characterized by severe motor and intellectual retardation, ataxia, hypotonia, epilepsy, absence of speech, and characteristic facies. The protein also interacts with the E6 protein of human papillomavirus types 16 and 18, resulting in ubiquitination and proteolysis of tumor protein p53. Alternative splicing of this gene results in three transcript variants encoding three isoforms with different N-termini. Additional transcript variants have been described, but their full length nature has not been determined. [provided by RefSeq, Jul 2008]'
Transcript Variant: This variant (2) encodes the longest isoform (2). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.

Other Versions

{0} Product Review(s)

0 Product Review(s) Submit review

Be the first one to submit a review

Product Citations

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.