CSB (ERCC6) (NM_000124) Human Untagged Clone

CAT#: SC309065

ERCC6 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 6 (ERCC6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ERCC6
• Synonyms: ARMD5; CKN2; COFS; COFS1; CSB; CSB-PGBD3; POF11; RAD26; UVSS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_000124, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCAAATGAGGGAATCCCCCACTCAAGTCAAACTCAGGAGCAAGACTGTTTACAGAGTCAACCTGTCA
  GTAATAATGAAGAAATGGCAATCAAGCAAGAAAGTGGTGGTGATGGGGAGGTGGAGGAGTACCTCTCCTT
  TCGTTCTGTGGGTGACGGGCTGTCCACCTCTGCTGTGGGGTGCGCATCAGCAGCTCCGAGGAGAGGGCCA
  GCCCTGCTGCACATCGACCGACATCAGATCCAGGCAGTAGAGCCTAGCGCCCAGGCCCTTGAGCTGCAGG
  GTTTGGGTGTGGACGTCTATGACCAGGACGTGCTGGAACAGGGAGTGCTTCAGCAGGTGGACAATGCCAT
  CCATGAGGCCAGCCGTGCCTCCCAGCTCGTTGACGTGGAGAAGGAGTATCGGTCGGTCCTGGATGACCTC
  ACGTCATGTACGACATCCCTAAGGCAAATCAATAAAATTATTGAACAGCTTAGCCCTCAAGCTGCCACCA
  GCAGAGACATCAACAGGAAACTAGATTCTGTAAAACGACAGAAGTATAATAAGGAACAACAGCTAAAAAA
  GATCACTGCAAAACAAAAGCATCTCCAGGCCATCCTTGGAGGAGCAGAGGTGAAAATTGAACTAGATCAC
  GCCAGTCTGGAGGAGGATGCAGAGCCGGGGCCATCCAGTCTTGGCAGCATGCTCATGCCTGTCCAGGAGA
  CTGCCTGGGAAGAGCTCATCCGCACTGGCCAGATGACACCTTTTGGTACCCAGATCCCTCAGAAACAGGA
  GAAAAAGCCCAGAAAAATCATGCTTAATGAAGCATCAGGCTTCGAAAAGTATTTGGCAGATCAAGCAAAA
  CTGTCTTTTGAAAGGAAGAAGCAAGGTTGTAATAAAAGAGCAGCTAGAAAAGCTCCAGCCCCAGTCACGC
  CTCCAGCCCCAGTGCAAAATAAAAACAAACCAAACAAGAAAGCCAGAGTTCTGTCCAAAAAAGAGGAGCG
  TTTGAAAAAGCACATCAAGAAACTCCAGAAGAGGGCTTTGCAGTTCCAGGGGAAAGTGGGATTGCCAAAG
  GCAAGGAGACCTTGGGAGTCAGACATGAGGCCAGAGGCAGAGGGAGACTCTGAGGGTGAAGAGTCTGAGT
  ATTTCCCCACAGAGGAGGAGGAAGAGGAGGAAGATGACGAGGTGGAGGGGGCAGAGGCGGACCTGTCTGG
  AGATGGTACTGACTATGAGCTGAAGCCTCTGCCCAAGGGCGGGAAACGGCAGAAGAAAGTGCCAGTGCAG
  GAGATTGATGATGACTTTTTCCCAAGTTCTGGGGAAGAAGCTGAAGCTGCTTCTGTAGGAGAAGGAGGAG
  GAGGAGGTCGGAAAGTGGGAAGATACCGAGATGATGGAGATGAAGATTATTATAAGCAGCGGTTAAGGAG
  ATGGAATAAACTGAGACTGCAGGACAAAGAGAAACGTCTGAAGCTGGAGGACGATTCTGAGGAAAGTGAT
  GCTGAATTTGACGAAGGTTTTAAAGTGCCAGGTTTTCTGTTCAAAAAGCTTTTTAAGTACCAGCAGACAG
  GTGTTAGGTGGCTGTGGGAATTGCACTGCCAGCAGGCAGGAGGAATTCTGGGAGATGAAATGGGATTGGG
  CAAGACCATCCAGATAATTGCCTTCTTGGCAGGTCTGAGCTACAGCAAGATCAGGACTCGTGGTTCAAAT
  TACAGGTTTGAGGGGTTGGGTCCAACTGTAATTGTCTGTCCAACAACAGTGATGCATCAGTGGGTGAAGG
  AATTTCACACGTGGTGGCCTCCGTTCAGAGTGGCAATTCTACATGAAACCGGTTCCTATACCCACAAAAA
  GGAGAAACTAATTCGAGATGTTGCTCATTGTCATGGAATTTTGATCACATCTTACTCCTACATTCGATTG
  ATGCAGGATGACATTAGCAGGTATGACTGGCACTATGTGATCTTGGACGAAGGACACAAAATTCGAAATC
  CAAATGCTGCTGTCACCCTTGCTTGCAAACAGTTTCGCACCCCTCATCGGATCATTCTGTCTGGCTCACC
  GATGCAAAATAACCTCCGAGAGCTGTGGTCGCTCTTTGACTTCATCTTCCCGGGAAAGTTAGGCACGTTG
  CCTGTGTTTATGGAGCAGTTCTCCGTCCCCATCACCATGGGGGGATATTCAAATGCTTCCCCAGTACAGG
  TCAAAACTGCTTACAAGTGTGCATGTGTCTTACGAGATACCATAAATCCATACCTACTGCGGAGAATGAA
  GTCAGATGTCAAGATGAGCCTTTCTTTGCCAGATAAAAATGAACAGGTCTTATTTTGCCGTCTTACAGAT
  GAGCAGCATAAAGTCTACCAAAATTTCGTTGATTCCAAAGAAGTTTACAGGATTCTCAATGGAGAGATGC
  AGATTTTCTCCGGACTTATAGCCCTAAGAAAAATTTGCAACCACCCTGATCTCTTTTCTGGAGGTCCCAA
  GAATCTCAAAGGTCTTCCTGATGATGAACTAGAAGAAGATCAGTTTGGGTACTGGAAACGTTCTGGGAAA
  ATGATTGTTGTTGAGTCTTTGTTGAAAATATGGCACAAGCAGGGTCAGCGAGTATTGCTGTTTTCTCAGT
  CAAGGCAGATGCTGGACATACTTGAAGTATTCCTTAGAGCCCAAAAGTATACCTATCTCAAGATGGATGG
  TACCACTACAATAGCTTCAAGACAGCCACTGATTACGAGATACAATGAGGACACATCCATATTTGTGTTT
  CTTCTGACCACGCGGGTGGGCGGCTTAGGTGTCAACCTGACGGGGGCAAACAGAGTTGTCATCTATGACC
  CAGACTGGAACCCAAGCACGGACACGCAGGCCCGGGAGCGAGCATGGAGAATAGGCCAGAAGAAGCAAGT
  GACTGTGTACAGGCTCCTGACTGCGGGCACCATTGAAGAAAAGATCTACCACCGACAAATCTTCAAGCAG
  TTTTTGACAAATAGAGTGCTAAAAGACCCAAAACAAAGGCGGTTTTTCAAATCCAATGATCTCTATGAGC
  TATTTACTCTGACTAGTCCTGATGCATCCCAGAGCACTGAAACAAGTGCAATTTTTGCAGGAACTGGATC
  AGATGTTCAGACACCCAAATGCCATCTAAAAAGAAGGATTCAACCAGCCTTTGGAGCAGACCATGATGTT
  CCAAAACGCAAGAAGTTCCCTGCTTCTAACATATCTGTAAATGATGCCACATCATCTGAAGAGAAATCTG
  AGGCTAAAGGAGCTGAAGTAAATGCAGTAACTTCTAATCGAAGTGATCCTTTGAAAGATGACCCTCACAT
  GAGTAGTAATGTAACTAGCAATGATAGGCTTGGAGAAGAGACAAATGCAGTATCTGGACCAGAAGAGTTG
  TCAGTGATTAGTGGAAATGGGGAATGTTCAAATTCTTCAGGAACAGGCAAAACTTCTATGCCATCTGGTG
  ATGAAAGCATTGATGAAAAGTTAGGTCTTTCTTACAAAAGAGAAAGACCCAGCCAGGCTCAAACAGAAGC
  TTTTTGGGAGAATAAACAAATGGAAAATAATTTTTATAAGCACAAGTCAAAAACAAAACATCATAGTGTG
  GCAGAAGAAGAGACCCTGGAGAAACATCTGAGACCAAAGCAAAAGCCTAAGAACTCTAAGCATTGCAGAG
  ACGCCAAGTTTGAAGGAACTCGAATTCCACACCTGGTGAAGAAAAGGCGTTACCAGAAGCAAGACAGTGA
  AAACAAGAGTGAGGCCAAGGAACAGAGCAATGACGATTATGTTTTGGAAAAGCTTTTCAAAAAATCAGTT
  GGCGTGCACAGTGTCATGAAGCACGATGCCATCATGGATGGAGCCAGCCCAGATTATGTACTGGTGGAGG
  CAGAAGCCAACCGAGTGGCCCAGGATGCCCTGAAAGCACTGAGGCTCTCTCGTCAGCGGTGTCTGGGAGC
  AGTGTCTGGTGTTCCCACCTGGACTGGCCACAGGGGGATTTCTGGTGCACCAGCAGGAAAAAAGAGTAGA
  TTTGGTAAGAAAAGGAATTCTAACTTCTCTGTGCAGCATCCTTCATCAACATCTCCAACAGAGAAGTGCC
  AGGATGGCATCATGAAAAAGGAGGGAAAAGATAATGTCCCTGAGCATTTTAGTGGAAGAGCAGAAGATGC
  AGACTCTTCATCCGGGCCCCTCGCTTCCTCCTCACTCTTGGCTAAAATGAGAGCTAGAAACCACCTGATT
  CTGCCAGAGCGTTTAGAAAGTGAAAGCGGGCACCTGCAGGAAGCTTCTGCCCTGCTGCCCACCACAGAAC
  ACGATGACCTTCTGGTGGAGATGAGAAACTTCATCGCTTTCCAGGCCCACACTGATGGCCAGGCCAGCAC
  CAGGGAGATACTGCAGGAGTTTGAATCCAAGTTATCTGCATCACAGTCTTGTGTCTTCCGAGAACTATTG
  AGAAATCTGTGCACTTTCCATAGAACTTCTGGTGGTGAAGGAATTTGGAAACTCAAGCCAGAATACTGCT
  AA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000124
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000124.3, NP_000115.1
• RefSeq Size: 8993 bp
• RefSeq ORF: 4482 bp
• Locus ID: 2074
• Cytogenetics: 10q11.23
• Domains: SNF2_N, DEAD, helicase_C
• Protein Families: Druggable Genome
• Protein Pathways: Nucleotide excision repair
• Gene Summary: 'This gene encodes a DNA-binding protein that is important in transcription-coupled excision repair. The encoded protein has ATP-stimulated ATPase activity, interacts with several transcription and excision repair proteins, and may promote complex formation at DNA repair sites. Mutations in this gene are associated with Cockayne syndrome type B and cerebrooculofacioskeletal syndrome 1. Alternative splicing occurs between a splice site from exon 5 of this gene to the 3' splice site upstream of the open reading frame (ORF) of the adjacent gene, piggyback-derived-3 (GeneID:267004), which activates the alternative polyadenylation site downstream of the piggyback-derived-3 ORF. The resulting transcripts encode a fusion protein that shares sequence with the product of each individual gene. [provided by RefSeq, Mar 2016]'
  Transcript Variant: This variant (3) represents the longest transcript and encodes the longer protein (isoform 2). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.