TRAF3 (NM_145725) Human Untagged Clone

CAT#: SC309131

TRAF3 (untagged)-Human TNF receptor-associated factor 3 (TRAF3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRAF3
• Synonyms: CAP-1; CAP1; CD40bp; CRAF1; IIAE5; LAP1; RNF118
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_145725 edited
  GAATTCGGCACGAGGCCCAGCCGGGACTTTCCAGCCGGCGGCAGCCGCGGCGGCCGCCGG
  CTCTTCCCCGCCCCCCGCCATGGGGCAGCCCGGGGAGCAGAACGCTGCGGACCGCGGCGG
  AGGACGCGCCCGGCGCCCCTGAGCCGGCCGAGCGGCGACGGACCGCGAGATGAGGAAAAT
  GAGGCCCAAAGAAGTGATGCCACTTGGTTAAGGTCCCAGAGCAGGTCAGAATCAGACCTA
  GGATCAGAAACCTGGCTCCTGGCTCCTGGCTCCCTACTCTTCTAAGGATCGCTGTCCTGA
  CAGAAGAGAACTCCTCTTTCCTAAAATGGAGTCGAGTAAAAAGATGGACTCTCCTGGCGC
  GCTGCAGACTAACCCGCCGCTAAAGCTGCACACTGACCGCAGTGCTGGGACGCCAGTTTT
  TGTCCCTGAACAAGGAGGTTACAAGGAAAAGTTTGTGAAGACCGTGGAGGACAAGTACAA
  GTGTGAGAAGTGCCACCTGGTGCTGTGCAGCCCGAAGCAGACCGAGTGTGGGCACCGCTT
  CTGCGAGAGCTGCATGGCGGCCCTGCTGAGCTCTTCAAGTCCAAAATGTACAGCGTGTCA
  AGAGAGCATCGTTAAAGATAAGGTGTTTAAGGATAATTGCTGCAAGAGAGAAATTCTGGC
  TCTTCAGATCTATTGTCGGAATGAAAGCAGAGGTTGTGCAGAGCAGTTAACGCTGGGACA
  TCTGCTGGTGCATTTAAAAAATGATTGCCATTTTGAAGAACTTCCATGTGTGCGTCCTGA
  CTGCAAAGAAAAGGTCTTGAGGAAAGACCTGCGAGACCACGTGGAGAAGGCGTGTAAATA
  CCGGGAAGCCACATGCAGCCACTGCAAGAGTCAGGTTCCGATGATCGCGCTGCAGAAACA
  CGAAGACACCGACTGTCCCTGCGTGGTGGTGTCCTGCCCTCACAAGTGCAGCGTCCAGAC
  TCTCCTGAGGAGCGAGTTGAGTGCACACTTGTCAGAGTGTGTCAATGCCCCCAGCACCTG
  TAGTTTTAAGCGCTATGGCTGCGTTTTTCAGGGGACAAACCAGCAGATCAAGGCCCACGA
  GGCCAGCTCCGCCGTGCAGCACGTCAACCTGCTGAAGGAGTGGAGCAACTCGCTCGAAAA
  GAAGGTTTCCTTGTTGCAGAATGAAAGTGTAGAAAAAAACAAGAGCATACAAAGTTTGCA
  CAATCAGATATGTAGCTTTGAAATTGAAATTGAGAGACAAAAGGAAATGCTTCGAAATAA
  TGAATCCAAAATCCTTCATTTACAGCGAGTGATAGACAGCCAAGCAGAGAAACTGAAGGA
  GCTTGACAAGGAGATCCGGCCCTTCCGGCAGAACTGGGAGGAAGCAGACAGCATGAAGAG
  CAGCGTGGAGTCCCTCCAGAACCGCGTGACCGAGCTGGAGAGCGTGGACAAGAGCGCGGG
  GCAAGTGGCTCGGAACACAGGCCTGCTGGAGTCCCAGCTGAGCCGGCATGACCAGATGCT
  GAGTGTGCACGACATCCGCCTAGCCGACATGGACCTGCGCTTCCAGGTCCTGGAGACCGC
  CAGCTACAATGGAGTGCTCATCTGGAAGATTCGCGACTACAAGCGGCGGAAGCAGGAGGC
  CGTCATGGGGAAGACCCTGTCCCTTTACAGCCAGCCTTTCTACACTGGTTACTTTGGCTA
  TAAGATGTGTGCCAGGGTCTACCTGAACGGGGACGGGATGGGGAAGGGGACGCACTTGTC
  GCTGTTTTTTGTCATCATGCGTGGAGAATATGATGCCCTGCTTCCTTGGCCGTTTAAGCA
  GAAAGTGACACTCATGCTGATGGATCAGGGGTCCTCTCGACGTCATTTGGGAGATGCATT
  CAAGCCCGACCCCAACAGCAGCAGCTTCAAGAAGCCCACTGGAGAGATGAATATCGCCTC
  TGGCTGCCCAGTCTTTGTGGCCCAAACTGTTCTAGAAAATGGGACATATATTAAAGATGA
  TACAATTTTTATTAAAGTCATAGTGGATACTTCGGATCTGCCCGATCCCTGATAAGTAGC
  TGGGGAGGTGGATTTAGCAGAAGGCAACTCCTCTGGGGGATTTGAACCGGTCTGTCTTCA
  CTGAGGTCCTCGCGCTCAGAAAAGGACCTTGTGAGACGGAGGAAGCGGCAGAAGGCGGAC
  GCGTGCCGGCGGGAGGAGCCACGCGTGAGCACACCTGACACGTTTTATAATAGACTAGCC
  ACACTTCACTCTGAAGAATTATTTATCCTTCAACAAGATAAATATTGCTGTCAGAGAAGG
  TTTTCATTTTCATTTTTAAAGATCTAGTTAATTAAGGTGGAAAACATATATGCTAAACAA
  AAGAAACATGATTTTTCTTCCTTAAACTTGAACACCAAAAAAACACACACACACACACAC
  GTGGGGATAGCTGGACATGTCAGCATGTTAAGTAAAAGGAGAATTTATGAAATAGTAATG
  CAATTCTGATATCTTCTTTCTAAAATTCAAGAGTGCAATTTTGTTTCAAATACAGTATAT
  TGTCTATTTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAACTCGAC
  
• Restriction Sites: Please inquire
• ACCN: NM_145725
• Insert Size: 2600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_145725.1, NP_663777.1
• RefSeq Size: 2567 bp
• RefSeq ORF: 1707 bp
• Locus ID: 7187
• Cytogenetics: 14q32.32
• Protein Families: Druggable Genome
• Protein Pathways: Pathways in cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, Toll-like receptor signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. The protein also plays a role in the regulation of antiviral response. Mutations in this are associated with Encephalopathy, acute, infection-induced, herpes-specific 5. [provided by RefSeq, Jul 2020]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Variants 1 and 3 both encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.