Raptor (RPTOR) (NM_020761) Human Untagged Clone

CAT#: SC309134

RPTOR (untagged)-Human regulatory associated protein of MTOR, complex 1 (RPTOR), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RPTOR
• Synonyms: KOG1; Mip1
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC309134 sequence for NM_020761 edited (data generated by NextGen Sequencing)
  ATGGAGTCCGAAATGCTGCAATCGCCTCTTCTGGGCCTGGGGGAGGAAGATGAGGCTGAT
  CTTACAGACTGGAACCTACCTTTGGCTTTTATGAAAAAGAGGCACTGTGAGAAAATTGAA
  GGCTCCAAATCCTTAGCTCAGAGCTGGAGGATGAAGGATCGGATGAAGACAGTCAGTGTT
  GCCTTAGTTTTGTGCCTGAATGTTGGTGTGGACCCTCCCGATGTGGTGAAGACCACGCCC
  TGTGCACGCTTGGAATGCTGGATCGATCCTCTGTCGATGGGTCCTCAGAAAGCTCTGGAA
  ACCATCGGTGCAAATTTACAGAAGCAGTACGAGAACTGGCAGCCAAGGGCCCGGTACAAG
  CAGAGCCTTGACCCAACTGTGGATGAAGTCAAGAAGCTCTGCACGTCCTTACGTCGCAAC
  GCCAAGGAGGAGCGAGTCCTCTTTCACTACAATGGCCACGGGGTGCCCCGGCCCACAGTC
  AACGGGGAGGTCTGGGTCTTCAACAAGAACTACACGCAGTACATCCCTCTGTCCATATAT
  GACCTGCAGACGTGGATGGGCAGCCCGTCGATCTTCGTCTACGACTGCTCCAATGCTGGC
  TTGATCGTCAAGTCCTTCAAGCAGTTCGCACTACAGCGGGAGCAGGAGCTGGAGGTAGCT
  GCAATCAACCCAAATCACCCTCTTGCTCAGATGCCTTTGCCTCCGTCGATGAAAAACTGC
  ATCCAGCTGGCAGCCTGCGAGGCCACCGAGCTGCTGCCCATGATCCCCGACCTCCCGGCT
  GACCTATTCACCTCCTGCCTCACCACCCCCATCAAGATCGCCCTGCGCTGGTTTTGCATG
  CAGAAATGTGTCAGTCTGGTGCCTGGCGTCACACTGGATTTGATAGAAAAGATCCCTGGC
  CGCCTGAACGACAGGAGGACGCCCCTGGGTGAACTGAACTGGATCTTCACAGCCATCACA
  GACACCATCGCGTGGAACGTGCTCCCCCGGGATCTCTTCCAAAAGCTCTTCAGACAGGAC
  TTGCTGGTGGCTAGTCTGTTTCGAAATTTTTTATTGGCGGAAAGGATTATGAGGTCGTAT
  AACTGCACTCCCGTCAGCAGCCCGCGTCTGCCGCCCACGTACATGCACGCCATGTGGCAA
  GCCTGGGACCTGGCTGTTGACATCTGTCTGTCTCAGCTGCCGACGATCATCGAGGAAGGC
  ACTGCGTTTCGGCACAGCCCGTTCTTCGCCGAGCAGCTGACCGCATTCCAGGTGTGGCTC
  ACCATGGGTGTGGAGAACCGAAACCCACCCGAACAGCTGCCCATCGTCCTGCAGGTGCTG
  TTAAGCCAAGTGCACCGGCTGAGAGCATTGGACTTGCTTGGAAGATTTTTGGACCTGGGT
  CCCTGGGCAGTGAGCCTGGCCTTGTCTGTCGGCATCTTCCCCTACGTGCTGAAGCTGCTC
  CAGAGCTCGGCCCGAGAGCTGCGGCCACTTCTCGTTTTCATCTGGGCCAAGATCCTCGCA
  GTGGACAGCTCGTGCCAAGCGGACCTCGTGAAGGACAACGGCCACAAGTACTTCCTGTCG
  GTCCTGGCGGACCCCTACATGCCAGCTGAACACCGGACCATGACGGCTTTCATTCTCGCC
  GTGATCGTCAACAGCTATCACACGGGGCAGGAAGCCTGCCTTCAGGGAAACCTCATTGCC
  ATCTGCCTGGAGCAGCTCAACGACCCGCACCCCTTGCTGCGCCAGTGGGTGGCCATCTGC
  CTCGGCAGGATCTGGCAGAACTTCGACTCGGCGAGGTGGTGCGGCGTGAGGGACAGCGCT
  CATGAGAAGCTCTACAGCCTCCTCTCCGACCCCATTCCCGAGGTCCGCTGCGCAGCGGTC
  TTCGCCCTTGGCACGTTCGTGGGCAACTCTGCAGAGAGGACGGACCACTCCACCACCATC
  GACCACAACGTGGCCATGATGCTGGCCCAGCTGGTCAGCGACGGGAGCCCCATGGTCCGG
  AAGGAGCTGGTGGTGGCTCTGAGTCATCTTGTGGTTCAGTATGAAAGCAATTTCTGCACC
  GTGGCCCTGCAGTTCATAGAAGAGGAAAAGAACTACGCCTTGCCTTCTCCAGCAACCACA
  GAGGGAGGGAGTTTGACCCCAGTGCGAGACAGCCCGTGCACCCCCAGACTTCGTTCTGTG
  AGCTCCTATGGAAACATCCGTGCTGTCGCCACAGCCAGGAGCCTCAACAAATCTTTGCAG
  AACCTGAGTTTGACAGAGGAATCTGGTGGCGCGGTGGCGTTCTCCCCCGGAAACCTCAGC
  ACCAGCAGCAGCGCCAGCAGCACCCTGGGCAGCCCCGAGAATGAGGAGCATATCCTGTCC
  TTCGAGACCATCGACAAGATGCGCCGCGCCAGCTCCTACTCCTCCCTCAACTCCCTCATC
  GGAGTTTCCTTTAACAGTGTTTACACTCAGATTTGGAGAGTCCTGCTGCACCTGGCTGCT
  GACCCCTATCCAGAGGTCTCGGACGTGGCCATGAAAGTACTCAACAGCATCGCCTACAAG
  GCCACCGTGAACGCCCGGCCGCAGCGCGTCCTGGACACCTCCTCCCTCACGCAGTCGGCC
  CCCGCCAGCCCCACCAACAAGGGCGTGCACATCCACCAGGCGGGGGGCTCCCCTCCGGCG
  TCCAGCACCAGCAGCTCCAGCCTGACCAACGATGTGGCCAAGCAGCCGGTCAGCCGAGAC
  TTGCCTTCTGGCCGGCCGGGCACCACAGGCCCCGCTGGGGCGCAGTACACCCCTCACTCC
  CACCAGTTCCCCCGGACACGGAAGATGTTCGACAAGGGCCCAGAGCAGACTGCGGACGAC
  GCGGACGATGCTGCTGGACACAAAAGTTTCATCTCCGCCACGGTGCAGACGGGGTTCTGC
  GACTGGAGCGCCCNNNNNNNNNCCCAGCCCGTCATGAAGATCCCAGAAGAGCACGACCTG
  GAGAGTCAGATCCGCAAGGAGCGGGAGTGGCGGTTCCTGCGAAACAGCCGTGTCAGGAGG
  CAGGCCCAGCAAGTCATTCAGAAGGGCATTACGAGATTGGACGACCAAATATTTCTGAAC
  AGGAACCCCGGCGTCCCCTCTGTGGTGAAATTCCACCCCTTCACGCCGTGCATCGCCGTA
  GCCGACAAGGACAGCATCTGCTTTTGGGACTGGGAGAAAGGGGAGAAGCTGGATTATTTC
  CACAATGGGAACCCTCGGTACACGAGGGTCACTGCCATGGAGTATCTGAACGGCCAGGAC
  TGCTCGCTTCTGCTGACGGCCACAGACGATGGTGCCATCAGGGTCTGGAAGAATTTTGCT
  GATTTGGAAAAGAACCCAGAGATGGTGACCGCGTGGCAGGGGCTCTCGGACATGCTGCCA
  ACGACGCGAGGAGCTGGGATGGTGGTGGACTGGGAGCAGGAGACCGGCCTCCTCATGAGC
  TCAGGAGACGTGCGGATCGTCCGGATCTGGGACACAGACCGTGAGATGAAGGTGCAGGAC
  ATCCCTACGGGCGCAGACAGCTGTGTGACGAGTCTGTCCTGTGATTCCCACCGCTCACTC
  ATCGTGGCTGGCCTCGGTGACGGCTCCATCCGCGTCTACGACAGAAGGATGGCACTCAGC
  GAATGCCGTGTCATGACGTACCGGGAGCACACAGCCTGGGTGGTGAAGGCCTCCCTGCAG
  AAGCGTCCCGACGGCCACATCGTGAGTGTGAGCGTCAATGGAGATGTGCGCATCTTTGAT
  CCCCGGATGCCTGAGTCGGTAAATGTGCTTCAGATCGTGAAGGGGCTGACGGCCCTGGAC
  ATCCACCCCCAGGCGGACCTGATCGCATGTGGCTCCGTCAATCAGTTCACCGCCATCTAC
  AACAGCAGCGGAGAGCTCATCAACAACATCAAGTACTACGACGGCTTCATGGGCCAGCGG
  GTCGGCGCCATCAGCTGCCTGGCCTTCCACCCGCACTGGCCTCACCTGGCCGTGGGAAGC
  AACGACTACTACATCTCCGTGTACTCGGTGGAGAAGCGTGTCAGATAG
  
  Clone variation with respect to NM_020761.2
  1269 c=>t;2894 g=>n;2895 c=>n;2896 t=>n;2897 a=>n;2898 t=>n;2899 t=>n;2900 t=>n;2901 t=>n;2902 g=>n;3609 c=>t
• Restriction Sites: Please inquire
• ACCN: NM_020761
• ORF Size: 4008 bp
• Insert Size: 6700
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.

Reference Data

• RefSeq: NM_020761.1, NP_065812.1
• RefSeq Size: 6431
• RefSeq ORF: 4008
• Locus ID: 57521
• Domains: WD40
• Protein Pathways: Insulin signaling pathway, mTOR signaling pathway
• Gene Summary: This gene encodes a component of a signaling pathway that regulates cell growth in response to nutrient and insulin levels. The encoded protein forms a stoichiometric complex with the mTOR kinase, and also associates with eukaryotic initiation factor 4E-binding protein-1 and ribosomal protein S6 kinase. The protein positively regulates the downstream effector ribosomal protein S6 kinase, and negatively regulates the mTOR kinase. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).