PAX3 (NM_181457) Human Untagged Clone

CAT#: SC309286

PAX3 (untagged)-Human paired box 3 (PAX3), transcript variant PAX3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PAX3
• Synonyms: CDHS; HUP2; WS1; WS3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_181457 edited
  GCCCTTAGTCGCTGGAACATTTGCCCAGACTCGTACCAAACTTTTCCGCCCTGGGCTCGG
  GATCCTGGACTCCGGGGCCTCCCCGTCCTCCCCTTTCCCGGGTTCCAGCTCCGGCCTCTG
  GACTAGGAACCGACAGCCCCCCTCCCCGCGTCCCTCCCTCTCTCTCCAGCCGTTTTGGGG
  AGGGGCTCTCCACGCTCCGGATAGTTCCCGAGGGTCATCCGCGCCGCACTCGCCTTTCCG
  TTTCGCCTTCACCTGGATATAATTTCCGAGCGAAGCTGCCCCCAGGATGACCACGCTGGC
  CGGCGCTGTGCCCAGGATGATGCGGCCGGGCCCGGGGCAGAACTACCCGCGTAGCGGGTT
  CCCGCTGGAAGTGTCCACTCCCCTCGGCCAGGGCCGCGTCAACCAGCTCGGCGGTGTTTT
  TATCAACGGCAGGCCGCTGCCCAACCACATCCGCCACAAGATCGTGGAGATGGCCCACCA
  CGGCATCCGGCCCTGCGTCATCTCGCGCCAGCTGCGCGTGTCCCACGGCTGCGTCTCCAA
  GATCCTGTGCAGGTACCAGGAGACTGGCTCCATACGTCCTGGTGCCATCGGCGGCAGCAA
  GCCCAAGCAGGTGACAACGCCTGACGTGGAGAAGAAAATTGAGGAATACAAAAGAGAGAA
  CCCGGGCATGTTCAGCTGGGAAATCCGAGACAAATTACTCAAGGACGCGGTCTGTGATCG
  AAACACCGTGCCGTCAGTGAGTTCCATCAGCCGCATCCTGAGAAGTAAATTCGGGAAAGG
  TGAAGAGGAGGAGGCCGACTTGGAGAGGAAGGAGGCAGAGGAAAGCGAGAAGAAGGCCAA
  ACACAGCATCGACGGCATCCTGAGCGAGCGAGCCTCAGCACCCCAATCAGATGAAGGCTC
  TGATATTGACTCTGAACCAGATTTACCACTAAAGAGGAAACAGCGCAGAAGCCGAACCAC
  CTTCACAGCAGAACAGCTGGAGGAACTGGAGCGTGCTTTTGAGAGAACTCATTACCCTGA
  CATTTATACTAGGGAGGAACTGGCCCAGAGGGCGAAGCTCACCGAGGCCCGAGTACAGGT
  CTGGTTTAGCAACCGCCGTGCAAGATGGAGGAAGCAAGCTGGGGCCAATCAACTGATGGC
  TTTCAACCATCTCATTCCCGGGGGGTTCCCTCCCACTGCCATGCCGACCTTGCCAACGTA
  CCAGCTGTCGGAGACCTCTTACCAGCCCACATCTATTCCACAAGCTGTGTCAGATCCCAG
  CAGCACCGTTCACAGACCTCAACCGCTTCCTCCAAGCACTGTACACCAAAGCACGATTCC
  TTCCAACCCAGACAGCAGCTCTGCCTACTGCCTCCCCAGCACCAGGCATGGATTTTCCAG
  CTATACAGACAGCTTTGTGCCTCCGTCGGGGCCCTCCAACCCCATGAACCCCACCATTGG
  CAATGGCCTCTCACCTCAGGTAATGGGACTCCTGACCAACCACGGTGGGGTACCTCATCA
  GCCCCAGACTGATTACGCGCTCTCCCCTCTCACCGGGGGTCTGGAACCTACCACCACGGT
  GTCGGCCAGCTGCAGTCAGAGACTAGACCATATGAAGAGCTTGGACAGTCTGCCAACATC
  TCAGTCCTACTGTCCACCCACCTATAGCACCACAGGCTACAGTATGGACCCTGTCACAGG
  CTACCAATATGGGCAGTATGGACAAAGTAAGCCTTGGACTTTTTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_181457
• Insert Size: 1900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_181457.1, NP_852122.1
• RefSeq Size: 3193 bp
• RefSeq ORF: 1440 bp
• Locus ID: 5077
• Cytogenetics: 2q36.1
• Protein Families: Adult stem cells, Druggable Genome, Embryonic stem cells, ES Cell Differentiation/IPS, Transcription Factors
• Gene Summary: 'This gene is a member of the paired box (PAX) family of transcription factors. Members of the PAX family typically contain a paired box domain and a paired-type homeodomain. These genes play critical roles during fetal development. Mutations in paired box gene 3 are associated with Waardenburg syndrome, craniofacial-deafness-hand syndrome, and alveolar rhabdomyosarcoma. The translocation t(2;13)(q35;q14), which represents a fusion between PAX3 and the forkhead gene, is a frequent finding in alveolar rhabdomyosarcoma. Alternative splicing results in transcripts encoding isoforms with different C-termini. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (PAX3) encodes isoform PAX3 (also known as isoform PAX3c). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.