EIF4G1 (NM_182917) Human Untagged Clone

CAT#: SC309331

EIF4G1 (untagged)-Human eukaryotic translation initiation factor 4 gamma, 1 (EIF4G1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF4G1
• Synonyms: EIF-4G1; EIF4F; EIF4G; EIF4GI; P220; PARK18
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_182917, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAACAAAGCTCCACAGTCCACAGGCCCCCCACCCGCCCCATCCCCCGGACTCCCACAGCCAGCGTTTC
  CCCCGGGGCAGACAGCGCCGGTGGTGTTCAGTACGCCACAAGCGACACAAATGAACACGCCTTCTCAGCC
  CCGCCAGCACTTCTACCCTAGCCGGGCCCAGCCCCCGAGCAGTGCAGCCTCCCGAGTGCAGAGTGCAGCC
  CCTGCCCGCCCTGGCCCAGCTGCCCATGTCTACCCTGCTGGATCCCAAGTAATGATGATCCCTTCCCAGA
  TCTCCTACCCAGCCTCCCAGGGGGCCTACTACATCCCTGGACAGGGGCGTTCCACATACGTTGTCCCGAC
  ACAGCAGTACCCTGTGCAGCCAGGAGCCCCAGGCTTCTATCCAGGTGCAAGCCCTACAGAATTTGGGACC
  TACGCTGGCGCCTACTATCCAGCCCAAGGGGTGCAGCAGTTTCCCACTGGCGTGGCCCCCGCCCCAGTTT
  TGATGAACCAGCCACCCCAGATTGCTCCCAAGAGGGAGCGTAAGACGATCCGAATTCGAGATCCAAACCA
  AGGAGGAAAGGATATCACAGAGGAGATCATGTCTGGGGCCCGCACTGCCTCCACACCCACCCCTCCCCAG
  ACGGGAGGCGGTCTGGAGCCTCAAGCTAATGGGGAGACGCCCCAGGTTGCTGTCATTGTCCGGCCAGATG
  ACCGGTCACAGGGAGCAATCATTGCTGACCGGCCAGGGCTGCCTGGCCCAGAGCATAGCCCTTCAGAATC
  CCAGCCTTCGTCGCCTTCTCCGACCCCATCACCATCCCCAGTCTTGGAACCGGGGTCTGAGCCTAATCTC
  GCAGTCCTCTCTATTCCTGGGGACACTATGACAACTATACAAATGTCTGTAGAAGAATCAACCCCCATCT
  CCCGTGAAACTGGGGAGCCATATCGCCTCTCTCCAGAACCCACTCCTCTCGCCGAACCCATACTGGAAGT
  AGAAGTGACACTTAGCAAACCGGTTCCAGAATCTGAGTTTTCTTCCAGTCCTCTCCAGGCTCCCACCCCT
  TTGGCATCTCACACAGTGGAAATTCATGAGCCTAATGGCATGGTCCCATCTGAAGATCTGGAACCAGAGG
  TGGAGTCAAGCCCAGAGCTTGCTCCTCCCCCAGCTTGCCCCTCCGAATCCCCTGTGCCCATTGCTCCAAC
  TGCCCAACCTGAGGAACTGCTCAACGGAGCCCCCTCGCCACCAGCTGTGGACTTAAGCCCAGTCAGTGAG
  CCAGAGGAGCAGGCCAAGGAGGTGACAGCATCAATGGCGCCCCCCACCATCCCCTCTGCTACTCCAGCTA
  CGGCTCCTTCAGCTACTTCCCCAGCTCAGGAGGAGGAAATGGAAGAAGAAGAAGAAGAGGAAGAAGGAGA
  AGCAGGAGAAGCAGGAGAAGCTGAGAGTGAGAAAGGAGGAGAGGAACTGCTCCCCCCAGAGAGTACCCCT
  ATTCCAGCCAACTTGTCTCAGAATTTGGAGGCAGCAGCAGCCACTCAAGTGGCAGTATCTGTGCCAAAGA
  GGAGACGGAAAATTAAGGAGCTAAATAAGAAGGAGGCTGTTGGAGACCTTCTGGATGCCTTCAAGGAGGC
  GAACCCGGCAGTACCAGAGGTGGAAAATCAGCCTCCTGCAGGCAGCAATCCAGGCCCAGAGTCTGAGGGC
  AGTGGTGTGCCCCCACGTCCTGAGGAAGCAGATGAGACCTGGGACTCAAAGGAAGACAAAATTCACAATG
  CTGAGAACATCCAGCCCGGGGAACAGAAGTATGAATATAAGTCAGATCAGTGGAAGCCTCTAAACCTAGA
  GGAGAAAAAACGTTACGACCGTGAGTTCCTGCTTGGTTTTCAGTTCATCTTTGCCAGTATGCAGAAGCCA
  GAGGGATTGCCACATATCAGTGACGTGGTGCTGGACAAGGCCAATAAAACACCACTGCGGCCACTGGATC
  CCACTAGACTACAAGGCATAAATTGTGGCCCAGACTTCACTCCATCCTTTGCCAACCTTGGCCGGACAAC
  CCTTAGCACCCGTGGGCCCCCAAGGGGTGGGCCAGGTGGGGAGCTGCCCCGTGGGCCGCAGGCTGGCCTG
  GGACCCCGGCGCTCTCAGCAGGGACCCCGAAAAGAACCACGCAAGATCATTGCCACAGTGTTAATGACCG
  AAGATATAAAACTGAACAAAGCAGAGAAAGCCTGGAAACCCAGCAGCAAGCGGACGGCGGCTGATAAGGA
  TCGAGGGGAAGAAGATGCTGATGGCAGCAAAACCCAGGACCTATTCCGCAGGGTGCGCTCCATCCTGAAT
  AAACTGACACCCCAGATGTTCCAGCAGCTGATGAAGCAAGTGACGCAGCTGGCCATCGACACCGAGGAAC
  GCCTCAAAGGGGTCATTGACCTCATTTTTGAGAAGGCCATTTCAGAGCCCAACTTCTCTGTGGCCTATGC
  CAACATGTGCCGCTGCCTCATGGCGCTGAAAGTGCCCACTACGGAAAAGCCAACAGTGACTGTGAACTTC
  CGAAAGCTGTTGTTGAATCGATGTCAGAAGGAGTTTGAGAAAGACAAAGATGATGATGAGGTTTTTGAGA
  AGAAGCAAAAAGAGATGGATGAAGCTGCTACGGCAGAGGAACGAGGACGCCTGAAGGAAGAGCTGGAAGA
  GGCTCGGGACATAGCCCGGCGGCGCTCTTTAGGGAATATCAAGTTTATTGGAGAGTTGTTCAAACTGAAG
  ATGTTAACAGAGGCAATAATGCATGACTGTGTGGTCAAACTGCTTAAGAACCATGATGAAGAGTCCCTTG
  AGTGCCTTTGTCGTCTGCTCACCACCATTGGCAAAGACCTGGACTTTGAAAAAGCCAAGCCCCGAATGGA
  TCAGTATTTCAACCAGATGGAAAAAATCATTAAAGAAAAGAAGACGTCATCCCGCATCCGCTTTATGCTG
  CAGGACGTGCTGGATCTGCGAGGGAGCAATTGGGTGCCACGCCGAGGGGATCAGGGTCCCAAGACCATTG
  ACCAGATCCATAAGGAGGCTGAGATGGAAGAACATCGAGAGCACATCAAAGTGCAGCAGCTCATGGCCAA
  GGGCAGTGACAAGCGTCGGGGCGGTCCTCCAGGCCCTCCCATCAGCCGTGGACTTCCCCTTGTGGATGAT
  GGTGGCTGGAACACAGTTCCCATCAGCAAAGGTAGCCGCCCCATTGACACCTCACGACTCACCAAGATCA
  CCAAGCCTGGCTCCATCGATTCTAACAACCAGCTCTTTGCACCTGGAGGGCGACTGAGCTGGGGCAAGGG
  CAGCAGCGGAGGCTCAGGAGCCAAGCCCTCAGACGCAGCATCAGAAGCTGCTCGCCCAGCTACTAGTACT
  TTGAATCGCTTCTCAGCCCTTCAACAAGCGGTACCCACAGAAAGCACAGATAATAGACGTGTGGTGCAGA
  GGAGTAGCTTGAGCCGAGAACGAGGCGAGAAAGCTGGAGACCGAGGAGACCGCCTAGAGCGGAGTGAACG
  GGGAGGGGACCGTGGGGACCGGCTTGATCGTGCGCGGACACCTGCTACCAAGCGGAGCTTCAGCAAGGAA
  GTGGAGGAGCGGAGTAGAGAACGGCCCTCCCAGCCTGAGGGGCTGCGCAAGGCAGCTAGCCTCACGGAGG
  ATCGGGACCGTGGGCGGGATGCCGTGAAGCGAGAAGCTGCCCTACCCCCAGTGAGCCCCCTGAAGGCGGC
  TCTCTCTGAGGAGGAGTTAGAGAAGAAATCCAAGGCTATCATTGAGGAATATCTCCATCTCAATGACATG
  AAAGAGGCAGTCCAGTGCGTGCAGGAGCTGGCCTCACCCTCCTTGCTCTTCATCTTTGTACGGCATGGTG
  TCGAGTCTACGCTGGAGCGCAGTGCCATTGCTCGTGAGCATATGGGGCAGCTGCTGCACCAGCTGCTCTG
  TGCTGGGCATCTGTCTACTGCTCAGTACTACCAAGGGTTGTATGAAATCTTGGAATTGGCTGAGGACATG
  GAAATTGACATCCCCCACGTGTGGCTCTACCTAGCGGAACTGGTAACACCCATTCTGCAGGAAGGTGGGG
  TGCCCATGGGGGAGCTGTTCAGGGAGATTACAAAGCCTCTGAGACCGTTGGGCAAAGCTGCTTCCCTGTT
  GCTGGAGATCCTGGGCCTCCTGTGCAAAAGCATGGGTCCTAAAAAGGTGGGGACGCTGTGGCGAGAAGCC
  GGGCTTAGCTGGAAGGAATTTCTACCTGAAGGCCAGGACATTGGTGCATTCGTCGCTGAACAGAAGGTGG
  AGTATACCCTGGGAGAGGAGTCGGAAGCCCCTGGCCAGAGGGCACTCCCCTCCGAGGAGCTGAACAGGCA
  GCTGGAGAAGCTGCTGAAGGAGGGCAGCAGTAACCAGCGGGTGTTCGACTGGATAGAGGCCAACCTGAGT
  GAGCAGCAGATAGTATCCAACACGTTAGTTCGAGCCCTCATGACGGCTGTCTGCTATTCTGCAATTATTT
  TTGAGACTCCCCTCCGAGTGGACGTTGCAGTGCTGAAAGCGCGAGCGAAGCTGCTGCAGAAATACCTGTG
  TGACGAGCAGAAGGAGCTACAGGCGCTCTACGCCCTCCAGGCCCTTGTAGTGACCTTAGAACAGCCTCCC
  AACCTGCTGCGGATGTTCTTTGACGCACTGTATGACGAGGACGTGGTGAAGGAGGATGCCTTCTACAGTT
  GGGAGAGTAGCAAGGACCCCGCTGAGCAGCAGGGCAAGGGTGTGGCCCTTAAATCTGTCACAGCCTTCTT
  CAAGTGGCTCCGTGAAGCAGAGGAGGAGTCTGACCACAACTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_182917
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182917.4, NP_886553.3
• RefSeq Size: 5686 bp
• RefSeq ORF: 4803 bp
• Locus ID: 1981
• Cytogenetics: 3q27.1
• Protein Pathways: Viral myocarditis
• Gene Summary: 'The protein encoded by this gene is a component of the multi-subunit protein complex EIF4F. This complex facilitates the recruitment of mRNA to the ribosome, which is a rate-limiting step during the initiation phase of protein synthesis. The recognition of the mRNA cap and the ATP-dependent unwinding of 5'-terminal secondary structure is catalyzed by factors in this complex. The subunit encoded by this gene is a large scaffolding protein that contains binding sites for other members of the EIF4F complex. A domain at its N-terminus can also interact with the poly(A)-binding protein, which may mediate the circularization of mRNA during translation. Alternative splicing results in multiple transcript variants, some of which are derived from alternative promoter usage. [provided by RefSeq, Aug 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.