ADAR1 (ADAR) (NM_015841) Human Untagged Clone

CAT#: SC309439

ADAR (untagged)-Human adenosine deaminase, RNA-specific (ADAR), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ADAR
• Synonyms: ADAR1; AGS6; DRADA; DSH; DSRAD; G1P1; IFI-4; IFI4; K88DSRBP; P136
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015841 edited
  CTTGGGCCCGGCGCGCAGACCCGCGGAGTTTCCCGTGCCGACGCCCCGGGGCCACTTCCA
  GTGCGGAGTAGCGGAGGCGTGGGGGCCTCGAGGGGCTGGCGCGGCCCAGCGGTCGGGCCA
  GGGTCGTGCCGCCGGCGGGTCGGGCCGGGCAATGCCTCGCGGGCGCAATGAATCCGCGGC
  AGGGGTATTCCCTCAGCGGATACTACACCCATCCATTTCAAGGCTATGAGCACAGACAGC
  TCAGATACCAGCAGCCTGGGCCAGGATCTTCCCCCAGTAGTTTCCTGCTTAAGCAAATAG
  AATTTCTCAAGGGGCAGCTCCCAGAAGCACCGGTGATTGGAAAGCAGACACCGTCACTGC
  CACCTTCCCTCCCAGGACTCCGGCCAAGGTTTCCAGTACTACTTGCCTCCAGTACCAGAG
  GCAGGCAAGTGGACATCAGGGGTGTCCCCAGGGGCGTGCATCTCGGAAGTCAGGGGCTCC
  AGAGAGGGTTCCAGCATCCTTCACCACGTGGCAGGAGTCTGCCACAGAGAGGTGTTGATT
  GCCTTTCCTCACATTTCCAGGAACTGAGTATCTACCAAGATCAGGAACAAAGGATCTTAA
  AGTTCCTGGAAGAGCTTGGGGAAGGGAAGGCCACCACAGCACATGATCTGTCTGGGAAAC
  TTGGGACTCCGAAGAAAGAAATCAATCGAGTTTTATACTCCCTGGCAAAGAAGGGCAAGC
  TACAGAAAGAGGCAGGAACACCCCCTTTGTGGAAAATCGCGGTCTCCACTCAGGCTTGGA
  ACCAGCACAGCGGAGTGGTAAGACCAGACGGTCATAGCCAAGGAGCCCCAAACTCAGACC
  CGAGTTTGGAACCGGAAGACAGAAACTCCACATCTGTCTCAGAAGATCTTCTTGAGCCTT
  TTATTGCAGTCTCAGCTCAGGCTTGGAACCAGCACAGCGGAGTGGTAAGACCAGACAGTC
  ATAGCCAAGGATCCCCAAACTCAGACCCAGGTTTGGAACCTGAAGACAGCAACTCCACAT
  CTGCCTTGGAAGATCCTCTTGAGTTTTTAGACATGGCCGAGATCAAGGAGAAAATCTGCG
  ACTATCTCTTCAATGTGTCTGACTCCTCTGCCCTGAATTTGGCTAAAAATATTGGCCTTA
  CCAAGGCCCGAGATATAAATGCTGTGCTAATTGACATGGAAAGGCAGGGGGATGTCTATA
  GACAAGGGACAACCCCTCCCATATGGCATTTGACAGACAAGAAGCGAGAGAGGATGCAAA
  TCAAGAGAAATACGAACAGTGTTCCTGAAACCGCTCCAGCTGCAATCCCTGAGACCAGAA
  GAAACGCAGAGTTCCTCACCTGTAATATACCCACATCAAATGCCTCAAATAACATGGTAA
  CCACAGAAAAAGTGGAGAATGGGCAGGAACCTGTCATAAAGTTAGAAAACAGGCAAGAGG
  CCAGACCAGAACCAGCAAGACTGAAACCACCTGTTCATTACAATGGCCCCTCAAAAGCAG
  GGTATGTTGACTTTGAAAATGGCCAGTGGGCCACAGATGACATCCCAGATGACTTGAATA
  GTATCCGCGCAGCACCAGGTGAGTTTCGAGCCATCATGGAGATGCCCTCCTTCTACAGTC
  ATGGCTTGCCACGGTGTTCACCCTACAAGAAACTGACAGAGTGCCAGCTGAAGAACCCCA
  TCAGCGGGCTGTTAGAATATGCCCAGTTCGCTAGTCAAACCTGTGAGTTCAACATGATAG
  AGCAGAGTGGACCACCCCATGAACCTCGATTTAAATTCCAGGTTGTCATCAATGGCCGAG
  AGTTTCCCCCAGCTGAAGCTGGAAGCAAGAAAGTGGCCAAGCAGGATGCAGCTATGAAAG
  CCATGACAATTCTGCTAGAGGAAGCCAAAGCCAAGGACAGTGGAAAATCAGAAGAATCAT
  CCCACTATTCCACAGAGAAAGAATCAGAGAAGACTGCAGAGTCCCAGACCCCCACCCCTT
  CAGCCACATCCTTCTTTTCTGGGAAGAGCCCCGTCACCACACTGCTTGAGTGTATGCACA
  AATTGGGGAACTCCTGCGAATTCCGTCTCCTGTCCAAAGAAGGCCCTGCCCATGAACCCA
  AGTTCCAATACTGTGTTGCAGTGGGAGCCCAAACTTTCCCCAGTGTGAGTGCTCCCAGCA
  AGAAAGTGGCAAAGCAGATGGCCGCAGAGGAAGCCATGAAGGCCCTGCATGGGGAGGCGA
  CCAACTCCATGGCTTCTGATAACCAGGTCAGGAAGATTGGCGAGCTCGTGAGATACCTGA
  ACACCAACCCTGTGGGTGGCCTTTTGGAGTACGCCCGCTCCCATGGCTTTGCTGCTGAAT
  TCAAGTTGGTCGACCAGTCCGGACCTCCTCACGAGCCCAAGTTCGTTTACCAAGCAAAAG
  TTGGGGGTCGCTGGTTCCCAGCCGTCTGCGCACACAGCAAGAAGCAAGGCAAGCAGGAAG
  CAGCAGATGCGGCTCTCCGTGTCTTGATTGGGGAGAACGAGAAGGCAGAACGCATGGGTT
  TCACAGAGCTCCCTCTCACTGGCAGCACCTTCCATGACCAGATAGCCATGCTGAGCCACC
  GGTGCTTCAACACTCTGACTAACAGCTTCCAGCCCTCCTTGCTCGGCCGCAAGATTCTGG
  CCGCCATCATTATGAAAAAAGACTCTGAGGACATGGGTGTCGTCGTCAGCTTGGGAACAG
  GGAATCGCTGTGTGAAAGGAGATTCTCTCAGCCTAAAAGGAGAAACTGTCAATGACTGCC
  ATGCAGAAATAATCTCCCGGAGAGGCTTCATCAGGTTTCTCTACAGTGAGTTAATGAAAT
  ACAACTCCCAGACTGCGAAGGATAGTATATTTGAACCTGCTAAGGGAGGAGAAAAGCTCC
  AAATAAAAAAGACTGTGTCATTCCATCTGTATATCAGCACTGCTCCGTGTGGAGATGGCG
  CCCTCTTTGACAAGTCCTGCAGCGACCGTGCTATGGAAAGCACAGAATCCCGCCACTACC
  CTGTCTTCGAGAATCCCAAACAAGGAAAGCTCCGCACCAAGGTGGAGAACGGAGAAGGCA
  CAATCCCTGTGGAATCCAGTGACATTGTGCCTACGTGGGATGGCATTCGGCTCGGGGAGA
  GACTCCGTACCATGTCCTGTAGTGACAAAATCCTACGCTGGAACGTGCTGGGCCTGCAAG
  GGGCACTGTTGACCCACTTCCTGCAGCCCATTTATCTCAAATCTGTCACATTGGGTTACC
  TTTTCAGCCAAGGGCATCTGACCCGTGCTATTTGCTGTCGTGTGACAAGAGATGGGAGTG
  CATTTGAGGATGGACTACGACATCCCTTTATTGTCAACCACCCCAAGGTTGGCAGAGTCA
  GCATATATGATTCCAAAAGGCAATCCGGGAAGACTAAGGAGACAAGCGTCAACTGGTGTC
  TGGCTGATGGCTATGACCTGGAGATCCTGGACGGTACCAGAGGCACTGTGGATGGGCCAC
  GGAATGAATTGTCCCGGGTCTCCAAAAAGAACATTTTTCTTCTATTTAAGAAGCTCTGCT
  CCTTCCGTTACCGCAGGGATCTACTGAGACTCTCCTATGGTGAGGCCAAGAAAGCTGCCC
  GTGACTACGAGACGGCCAAGAACTACTTCAAAAAAGGCCTGAAGGATATGGGCTATGGGA
  ACTGGATTAGCAAACCCCAGGAGGAAAAGAACTTTTATCTCTGCCCAGTATAGTATGCTC
  CAGTGACAGATGGATTAGGGTGTGTCATACTAGGGTGTGAGAGAGGTAGGTCGTAGCATT
  CCTCATCACATGGTCAGGGGATTTTTTTTTCTCCTTTTTTTTTCTTTTTAAGCCATAATT
  GGTGATACTGAAAACTTTGGGTTCCCATTTATCCTGCTTTCTTTGGGATTGCTAGGCAAG
  GTCTGGCCAGGCCCCCCTTTTTTCCCCCAAGTGAAGAGGCAGAAACCTAAGAAGTTATCT
  TTTCTTTCTACCCAAAGCATACATAGTCACTGAGCACCTGCGGTCCATTTCCTCTTAAAA
  GTTTTGTTTTGATTTGTTTCCATTTCCTTTCCCTTTGTGTTTGCTACACTGACCTCTTGC
  GGTCTTGATTAGGTTTCAGTCAACTCTGGATCATGTCAGGGACTGATAATTTCATTTGTG
  GATTACGCAGACCCCTCTACTTCCCCTCTTTCCCTTCTGAGATTCTTTCCTTGTGATCTG
  AATGTCTCCTTTTCCCCCTCAGAGGGCAAAGAGGTGAACATAAAGGATTTGGTGAAACAT
  TTGTAAGGGTAGGAGTTGAAAACTGCAGTTCCCAGTGCCACGGAAGTGTGATTGGAGCCT
  GCAGATAATGCCCAGCCATCCTCCCATCCTGCACTTTAGCCAGCTGCAGGGCGGGCAAGG
  CAAGGAAAGCTGCTTCCCTGGAAGTGTATCACTTTCTCCGGCAGCTGGGAAGTCTAGAA
  
• Restriction Sites: Please inquire
• ACCN: NM_015841
• Insert Size: 4400 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: There are 2 nucleotide differences between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 1 amino acid.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015841.2, NP_056656.2
• RefSeq Size: 6505 bp
• RefSeq ORF: 3546 bp
• Locus ID: 103
• Cytogenetics: 1q21.3
• Domains: z-alpha, DSRM, A_deamin
• Protein Families: Druggable Genome
• Protein Pathways: Cytosolic DNA-sensing pathway
• Gene Summary: 'This gene encodes the enzyme responsible for RNA editing by site-specific deamination of adenosines. This enzyme destabilizes double-stranded RNA through conversion of adenosine to inosine. Mutations in this gene have been associated with dyschromatosis symmetrica hereditaria. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]'
  Transcript Variant: This variant (3) uses two alternate in-frame splice sites in the central coding region, compared to variant 1, resulting in an isoform (c, also referred to as ADAR-c) that is shorter than isoform a. There are no publicly available full-length transcripts representing this variant; it is represented based on data in PMID:9020165 and annotation on DNA accession U75503.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.