COL11A1 (NM_080629) Human Untagged Clone

CAT#: SC309482

COL11A1 (untagged)-Human collagen, type XI, alpha 1 (COL11A1), transcript variant B

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: COL11A1
• Synonyms: CO11A1; COLL6; STL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_080629, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCCGTGGTCCTCTAGGTGGAAAACGAAACGGTGGCTCTGGGATTTCACCGTAACAACCCTCGCAT
  TGACCTTCCTCTTCCAAGCTAGAGAGGTCAGAGGAGCTGCTCCAGTTGATGTACTAAAAGCACTAGATTT
  TCACAATTCTCCAGAGGGAATATCAAAAACAACGGGATTTTGCACAAACAGAAAGAATTCTAAAGGCTCA
  GATACTGCTTACAGAGTTTCAAAGCAAGCACAACTCAGTGCCCCAACAAAACAGTTATTTCCAGGTGGAA
  CTTTCCCAGAAGACTTTTCAATACTATTTACAGTAAAACCAAAAAAAGGAATTCAGTCTTTCCTTTTATC
  TATATATAATGAGCATGGTATTCAGCAAATTGGTGTTGAGGTTGGGAGATCACCTGTTTTTCTGTTTGAA
  GACCACACTGGAAAACCTGCCCCAGAAGACTATCCCCTCTTCAGAACTGTTAACATCGCTGACGGGAAGT
  GGCATCGGGTAGCAATCAGCGTGGAGAAGAAAACTGTGACAATGATTGTTGATTGTAAGAAGAAAACCAC
  GAAACCACTTGATAGAAGTGAGAGAGCAATTGTTGATACCAATGGAATCACGGTTTTTGGAACAAGGATT
  TTGGATGAAGAAGTTTTTGAGGGGGACATTCAGCAGTTTTTGATCACAGGTGATCCCAAGGCAGCATATG
  ACTACTGTGAGCATTATAGTCCAGACTGTGACTCTTCAGCACCCAAGGCTGCTCAAGCTCAGGAACCTCA
  GATAGATGAGAAAAAGAAATCCAATTTCAAAAAGAAGATGAGGACAGTGGCTACTAAATCAAAGGAAAAA
  TCCAAAAAGTTTACACCCCCCAAATCTGAAAAATTTTCATCCAAGAAGAAGAAAAGTTATCAAGCATCAG
  CAAAAGCCAAACTAGGGGTAAAGGCAAACATCGTTGATGATTTTCAAGAATACAACTATGGAACAATGGA
  AAGTTACCAGACAGAAGCTCCTAGGCATGTTTCTGGGACAAATGAGCCAAATCCAGTTGAAGAAATATTT
  ACTGAAGAATATCTAACGGGAGAGGATTATGATTCCCAGAGGAAAAATTCTGAGGATACACTATATGAAA
  ACAAAGAAATAGACGGCAGGGATTCTGATCTTCTGGTAGATGGAGATTTAGGCGAATATGATTTTTATGA
  ATATAAAGAATATGAAGATAAACCAACAAGCCCCCCTAATGAAGAATTTGGTCCAGGTGTACCAGCAGAA
  ACTGATATTACAGAAACAAGCATAAATGGCCATGGTGCATATGGAGAGAAAGGACAGAAAGGAGAACCAG
  CAGTGGTTGAGCCTGGTATGCTTGTCGAAGGACCACCAGGACCAGCAGGACCTGCAGGTATTATGGGTCC
  TCCAGGTCTACAAGGCCCCACTGGACCCCCTGGTGACCCTGGCGATAGGGGCCCCCCAGGACGTCCTGGC
  TTACCAGGGGCTGATGGTCTACCTGGTCCTCCTGGTACTATGTTGATGTTACCGTTCCGTTATGGTGGTG
  ATGGTTCCAAAGGACCAACCATCTCTGCTCAGGAAGCTCAGGCTCAAGCTATTCTTCAGCAGGCTCGGAT
  TGCTCTGAGAGGCCCACCTGGCCCAATGGGTCTAACTGGAAGACCAGGTCCTGTGGGGGGGCCTGGTTCA
  TCTGGGGCCAAAGGTGAGAGTGGTGATCCAGGTCCTCAGGGCCCTCGAGGCGTCCAGGGTCCCCCTGGTC
  CAACGGGAAAACCTGGAAAAAGGGGTCGTCCAGGTGCAGATGGAGGAAGAGGAATGCCAGGAGAACCTGG
  GGCAAAGGGAGATCGAGGGTTTGATGGACTTCCGGGTCTGCCAGGTGACAAAGGTCACAGGGGTGAACGA
  GGTCCTCAAGGTCCTCCAGGTCCTCCTGGTGATGATGGAATGAGGGGAGAAGATGGAGAAATTGGACCAA
  GAGGTCTTCCAGGTGAAGCTGGCCCACGAGGTTTGCTGGGTCCAAGGGGAACTCCAGGAGCTCCAGGGCA
  GCCTGGTATGGCAGGTGTAGATGGCCCCCCAGGACCAAAAGGGAACATGGGTCCCCAAGGGGAGCCTGGG
  CCTCCAGGTCAACAAGGGAATCCAGGACCTCAGGGTCTTCCTGGTCCACAAGGTCCAATTGGTCCTCCTG
  GTGAAAAAGGACCACAAGGAAAACCAGGACTTGCTGGACTTCCTGGTGCTGATGGGCCTCCTGGTCATCC
  TGGGAAAGAAGGCCAGTCTGGAGAAAAGGGGGCTCTGGGTCCCCCTGGTCCACAAGGTCCTATTGGATAC
  CCGGGCCCCCGGGGAGTAAAGGGAGCAGATGGTGTCAGAGGTCTCAAGGGATCTAAAGGTGAAAAGGGTG
  AAGATGGTTTTCCAGGATTCAAAGGTGACATGGGTCTAAAAGGTGACAGAGGAGAAGTTGGTCAAATTGG
  CCCAAGAGGGGAAGATGGCCCTGAAGGACCCAAAGGTCGAGCAGGCCCAACTGGAGACCCAGGTCCTTCA
  GGTCAAGCAGGAGAAAAGGGAAAACTTGGAGTTCCAGGATTACCAGGATATCCAGGAAGACAAGGTCCAA
  AGGGTTCCACTGGATTCCCTGGGTTTCCAGGTGCCAATGGAGAGAAAGGTGCACGGGGAGTAGCTGGCAA
  ACCAGGCCCTCGGGGTCAGCGTGGTCCAACGGGTCCTCGAGGTTCAAGAGGTGCAAGAGGTCCCACTGGG
  AAACCTGGGCCAAAGGGCACTTCAGGTGGCGATGGCCCTCCTGGCCCTCCAGGTGAAAGAGGTCCTCAAG
  GACCTCAGGGTCCAGTTGGATTCCCTGGACCAAAAGGCCCTCCTGGACCACCTGGGAAGGATGGGCTGCC
  AGGACACCCTGGGCAACGTGGGGAGACTGGATTTCAAGGCAAGACCGGCCCTCCTGGGCCAGGGGGAGTG
  GTTGGACCACAGGGACCAACCGGTGAGACTGGTCCAATAGGGGAACGTGGGCATCCTGGCCCTCCTGGCC
  CTCCTGGTGAGCAAGGTCTTCCTGGTGCTGCAGGAAAAGAAGGTGCAAAGGGTGATCCAGGTCCTCAAGG
  TATCTCAGGGAAAGATGGACCAGCAGGATTACGTGGTTTCCCAGGGGAAAGAGGTCTTCCTGGAGCTCAG
  GGTGCACCTGGACTGAAAGGAGGGGAAGGTCCCCAGGGCCCACCAGGTCCAGTTGGCTCACCAGGAGAAC
  GTGGGTCAGCAGGTACAGCTGGCCCAATTGGTTTACCAGGGCGCCCGGGACCTCAGGGTCCTCCTGGTCC
  AGCTGGAGAGAAAGGTGCTCCTGGAGAAAAAGGTCCCCAAGGGCCTGCAGGGAGAGATGGAGTTCAAGGT
  CCTGTTGGTCTCCCAGGGCCAGCTGGTCCTGCCGGCTCCCCTGGGGAAGACGGAGACAAGGGTGAAATTG
  GTGAGCCGGGACAAAAAGGCAGCAAGGGTGACAAGGGAGAAAATGGCCCTCCCGGTCCCCCAGGTCTTCA
  AGGACCAGTTGGTGCCCCTGGAATTGCTGGAGGTGATGGTGAACCAGGTCCTAGAGGACAGCAGGGGATG
  TTTGGGCAAAAAGGTGATGAGGGTGCCAGAGGCTTCCCTGGACCTCCTGGTCCAATAGGTCTTCAGGGTC
  TGCCAGGCCCACCTGGTGAAAAAGGTGAAAATGGGGATGTTGGTCCCATGGGGCCACCTGGTCCTCCAGG
  CCCAAGAGGCCCTCAAGGTCCCAATGGAGCTGATGGACCACAAGGACCCCCAGGGTCTGTTGGTTCAGTT
  GGTGGTGTTGGAGAAAAGGGTGAACCTGGAGAAGCAGGGAACCCAGGGCCTCCTGGGGAAGCAGGTGTAG
  GCGGTCCCAAAGGAGAAAGAGGAGAGAAAGGGGAAGCTGGTCCACCTGGAGCTGCTGGACCTCCAGGTGC
  CAAGGGGCCACCAGGTGATGATGGCCCTAAGGGTAACCCGGGTCCTGTTGGTTTTCCTGGAGATCCTGGT
  CCTCCTGGGGAACCTGGCCCTGCAGGTCAAGATGGTGTTGGTGGTGACAAGGGTGAAGATGGAGATCCTG
  GTCAACCGGGTCCTCCTGGCCCATCTGGTGAGGCTGGCCCACCAGGTCCTCCTGGAAAACGAGGTCCTCC
  TGGAGCTGCAGGTGCAGAGGGAAGACAAGGTGAAAAAGGTGCTAAGGGGGAAGCAGGTGCAGAAGGTCCT
  CCTGGAAAAACCGGCCCAGTCGGTCCTCAGGGACCTGCAGGAAAGCCTGGTCCAGAAGGTCTTCGGGGCA
  TCCCTGGTCCTGTGGGAGAACAAGGTCTCCCTGGAGCTGCAGGCCAAGATGGACCACCTGGTCCTATGGG
  ACCTCCTGGCTTACCTGGTCTCAAAGGTGACCCTGGCTCCAAGGGTGAAAAGGGACATCCTGGTTTAATT
  GGCCTGATTGGTCCTCCAGGAGAACAAGGGGAAAAAGGTGACCGAGGGCTCCCTGGAACTCAAGGATCTC
  CAGGAGCAAAAGGGGATGGGGGAATTCCTGGTCCTGCTGGTCCCTTAGGTCCACCTGGTCCTCCAGGTTT
  ACCAGGTCCTCAAGGCCCAAAGGGTAACAAAGGCTCTACTGGACCCGCTGGCCAGAAAGGTGACAGTGGT
  CTTCCAGGGCCTCCTGGGTCTCCAGGTCCACCTGGTGAAGTCATTCAGCCTTTACCAATCTTGTCCTCCA
  AAAAAACGAGAAGACATACTGAAGGCATGCAAGCAGATGCAGATGATAATATTCTTGATTACTCGGATGG
  AATGGAAGAAATATTTGGTTCCCTCAATTCCCTGAAACAAGACATTGAGCATATGAAATTTCCAATGGGT
  ACTCAGACCAATCCAGCCCGAACTTGTAAAGACCTGCAACTCAGCCATCCTGACTTCCCAGATGGTGAAT
  ATTGGATTGATCCTAACCAAGGTTGCTCAGGAGATTCCTTCAAAGTTTACTGTAATTTCACATCTGGTGG
  TGAGACTTGCATTTATCCAGACAAAAAATCTGAGGGAGTAAGAATTTCATCATGGCCAAAGGAGAAACCA
  GGAAGTTGGTTTAGTGAATTTAAGAGGGGAAAACTGCTTTCATACTTAGATGTTGAAGGAAATTCCATCA
  ATATGGTGCAAATGACATTCCTGAAACTTCTGACTGCCTCTGCTCGGCAAAATTTCACCTACCACTGTCA
  TCAGTCAGCAGCCTGGTATGATGTGTCATCAGGAAGTTATGACAAAGCACTTCGCTTCCTGGGATCAAAT
  GATGAGGAGATGTCCTATGACAATAATCCTTTTATCAAAACACTGTATGATGGTTGTGCGTCCAGAAAAG
  GCTATGAAAAGACTGTCATTGAAATCAATACACCAAAAATTGATCAAGTACCTATTGTTGATGTCATGAT
  CAATGACTTTGGTGATCAGAATCAGAAGTTCGGATTTGAAGTTGGTCCTGTTTGTTTTCTTGGCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_080629
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_080629.2, NP_542196.2
• RefSeq Size: 7327 bp
• RefSeq ORF: 5457 bp
• Locus ID: 1301
• Cytogenetics: 1p21.1
• Protein Pathways: ECM-receptor interaction, Focal adhesion
• Gene Summary: 'This gene encodes one of the two alpha chains of type XI collagen, a minor fibrillar collagen. Type XI collagen is a heterotrimer but the third alpha chain is a post-translationally modified alpha 1 type II chain. Mutations in this gene are associated with type II Stickler syndrome and with Marshall syndrome. A single-nucleotide polymorphism in this gene is also associated with susceptibility to lumbar disc herniation. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009]'
  Transcript Variant: This variant (B) utilizes alternate exon 6, designated exon 6B, and encodes the longest isoform (B). There are no publicly available transcripts representing this variant; it is supported by mRNA annotation on DNA accession AF101112.1, protein AAF04726.1, submitted by the authors of PMID:10486316. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The extent of this transcript is supported by transcript alignments.