BPTF (NM_004459) Human Untagged Clone

CAT#: SC309497

BPTF (untagged)-Human bromodomain PHD finger transcription factor (BPTF), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BPTF
• Synonyms: FAC1; FALZ; NEDDFL; NURF301
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_004459, the custom clone sequence may differ by one or more nucleotides
  ATGAGGGGCCGGCGGGGCAGGCCGCCCAAGCAGCCCGCGGCTCCCGCTGCGGAGCGCTGC
  GCCCCGGCCCCGCCGCCACCGCCGCCGCCGCCCACGTCCGGACCCATCGGGGGGCTCCGC
  TCGCGGCACCGCGGCAGCAGCCGGGGCAGGTGGGCCGCCGCCCAGGCTGAGGTGGCGCCC
  AAGACGCGGCTGAGCTCGCCCAGGGGGGGCAGCAGTAGCCGGAGGAAGCCGCCGCCGCCG
  CCGCCGGCCCCCCCCAGCACCAGCGCCCCGGGCCGGGGGGGGCGAGGAGGCGGGGGCGGC
  AGGACGGGGGGCGGGGGCGGCGGCGGCCACCTGGCCCGGACCACCGCGGCCCGGAGGGCC
  GTCAACAAAGTGGTGTACGATGACCACGAGAGCGAGGAGGAGGAGGAAGAGGAGGACATG
  GTCTCCGAGGAGGAGGAGGAGGAGGACGGCGACGCCGAGGAGACCCAGGATTCTGAGGAC
  GACGAGGAGGATGAGATGGAAGAGGACGACGATGACTCCGATTATCCGGAGGAGATGGAA
  GACGACGACGACGACGCCAGTTACTGCACGGAAAGCAGCTTCAGGAGCCATAGTACCTAC
  AGCAGCACTCCAGGTAGGCGAAAACCAAGAGTACATCGGCCTCGTTCTCCTATATTGGAA
  GAAAAAGACATCCCGCCCCTTGAATTTCCCAAGTCCTCTGAGGATTTAATGGTGCCTAAT
  GAGCATATAATGAATGTCATTGCCATTTACGAGGTACTGCGGAACTTTGGCACTGTTTTG
  AGATTATCTCCTTTTCGCTTTGAGGACTTTTGTGCAGCTCTGGTGAGCCAAGAGCAGTGC
  ACACTCATGGCAGAGATGCATGTTGTGCTTTTGAAAGCAGTTCTGCGTGAAGAAGACACT
  TCCAATACTACCTTTGGACCTGCTGATCTGAAAGATAGCGTTAATTCCACACTGTATTTC
  ATAGATGGGATGACGTGGCCAGAGGTGCTGCGGGTGTACTGTGAGAGTGATAAGGAGTAC
  CATCACGTTCTTCCTTACCAAGAGGCAGAGGACTACCCATATGGACCAGTAGAGAACAAG
  ATCAAAGTTCTACAGTTTCTAGTCGATCAGTTTCTTACAACAAATATTGCTCGAGAGGAA
  TTGATGTCTGAAGGGGTGATACAGTATGATGACCATTGTAGGGTTTGTCACAAACTTGGG
  GATTTGCTTTGCTGTGAGACATGTTCAGCAGTATACCATTTGGAATGTGTGAAGCCACCT
  CTTGAGGAGGTGCCAGAGGACGAGTGGCAGTGTGAAGTCTGTGTAGCACACAAGGTGCCT
  GGTGTGACTGACTGTGTTGCTGAAATCCAAAAAAATAAACCATATATTCGACATGAACCT
  ATTGGATATGATAGAAGTCGGAGGAAATACTGGTTCTTGAACCGAAGACTCATAATAGAA
  GAAGATACAGAAAATGAAAATGAAAAGAAAATTTGGTATTACAGCACAAAGGTCCAACTT
  GCAGAATTAATTGACTGTCTAGACAAAGATTATTGGGAAGCAGAACTCTGCAAAATTCTA
  GAAGAAATGCGTGAAGAAATCCACCGACACATGGACATAACTGAAGACCTGACCAATAAG
  GCTCGGGGCAGTAACAAATCCTTTCTGGCGGCAGCTAATGAAGAAATTTTGGAATCCATA
  AGAGCCAAAAAGGGAGACATTGATAATGTTAAAAGCCCAGAAGAAACAGAAAAAGACAAG
  AATGAGACTGAGAATGACTCTAAAGATGCTGAGAAAAACAGAGAAGAATTTGAAGACCAG
  TCCCTTGAAAAAGACAGTGACGACAAAACACCAGATGATGACCCTGAGCAAGGAAAATCT
  GAGGAGCCAACAGAAGTTGGGGATAAAGGTAACTCTGTGTCAGCAAATCTTGGCGACAAC
  ACAACAAATGCAACTTCAGAAGAGACTAGTCCCTCTGAAGGGAGGAGCCCTGTGGGGTGT
  CTCTCAGAAACCCCCGATAGCAGCAACATGGCAGAGAAGAAGGTGGCATCTGAGCTCCCC
  CAGGATGTGCCAGAAGAACCTAACAAGACATGTGAGAGCAGTAACACTAGTGCTACCACT
  ACCTCCATCCAGCCTAATCTGGAAAACAGTAACAGCAGCAGTGAACTAAATTCTTCCCAG
  AGTGAATCTGCTAAGGCAGCTGATGATCCTGAAAATGGAGAAAGAGAATCTCATACACCT
  GTCTCTATTCAGGAAGAGATAGTAGGTGATTTCAAATCGGAGAAGTCCAACGGGGAGCTA
  AGTGAATCTCCTGGAGCTGGAAAAGGAGCATCTGGCTCAACTCGAATCATCACCAGATTG
  CGGAATCCAGATAGCAAACTTAGTCAGCTGAAGAGCCAGCAGGTGGCAGCCGCTGCACAT
  GAAGCAAATAAATTATTTAAGGAGGGCAAAGAGGTACTGGTAGTTAACTCTCAAGGAGAA
  ATTTCACGGTTGAGCACCAAAAAGGAAGTGATCATGAAAGGAAATATCAACAATTATTTT
  AAATTGGGTCAAGAAGGGAAGTATCGCGTCTACCACAATCAATACTCCACCAATTCATTT
  GCTTTGAATAAGCACCAGCACAGAGAAGACCATGATAAGAGAAGGCATCTTGCACATAAG
  TTCTGTCTGACTCCAGCAGGAGAGTTCAAATGGAACGGTTCTGTCCATGGGTCCAAAGTT
  CTTACCATATCTACTCTGAGACTGACTATCACCCAATTAGAAAACAACATCCCTTCATCC
  TTTCTTCATCCCAACTGGGCATCACATAGGGCAAATTGGATCAAGGCAGTTCAGATGTGT
  AGCAAACCCAGAGAATTTGCATTGGCTTTAGCCATTTTGGAGTGTGCAGTTAAACCAGTT
  GTGATGCTACCAATATGGCGAGAATCTTTAGGACATACCAGGTTACACCGGATGACATCA
  ATTGAAAGAGAAGAAAAGGAGAAAGTCAAAAAAAAAGAGAAGAAACAGGAAGAAGAAGAA
  ACGATGCAGCAAGCGACATGGGTAAAATACACATTTCCAGTTAAGCATCAGGTTTGGAAA
  CAAAAAGGTGAAGAGTACAGAGTGACAGGATATGGTGGTTGGAGCTGGATTAGTAAAACT
  CATGTTTATAGGTTTGTTCCTAAATTGCCAGGCAATACTAATGTGAATTACAGAAAGTCG
  TTAGAAGGAACCAAAAATAATATGGATGAAAATATGGATGAGTCAGATAAAAGAAAATGT
  TCACGAAGTCCAAAAAAAATAAAAATAGAGCCTGATTCTGAAAAAGATGAGGTAAAAGGT
  TCAGATGCTGCAAAAGGAGCAGACCAAAATGAAATGGATATCTCAAAGATTACTGAGAAG
  AAGGACCAAGATGTGAAGGAGCTCTTAGATTCTGACAGTGATAAACCCTGCAAGGAAGAA
  CCAATGGAAGTAGACGATGACATGAAAACAGAGTCACATGTAAATTGTCAGGAGAGTTCT
  CAAGTAGATGTGGTCAATGTTAGTGAGGGTTTTCATCTAAGGACTAGTTACAAAAAGAAA
  ACAAAATCATCCAAACTAGATGGACTTCTTGAAAGGAGAATTAAACAGTTTACACTGGAA
  GAAAAACAGCGACTCGAAAAAATCAAGTTGGAGGGTGGAATTAAGGGTATAGGAAAGACT
  TCTACAAATTCTTCAAAAAATCTCTCTGAATCACCAGTAATAACGAAAGCAAAAGAAGGG
  TGTCAGAGTGACTCGATGAGACAAGAACAGAGCCCAAATGCAAATAATGATCAACCTGAG
  GACTTGATTCAGGGATGTTCAGAAAGTGATTCCTCAGTTCTTAGAATGAGTGATCCTAGT
  CATACCACAAACAAACTTTATCCAAAAGATCGAGTGTTAGATGATGTCTCCATTCGGAGC
  CCAGAAACAAAATGTCCGAAACAAAATTCCATTGAAAATGACATAGAAGAAAAAGTCTCT
  GACCTTGCCAGTAGAGGCCAGGAACCCAGTAAGAGTAAAACAAAAGGAAATGATTTTTTC
  ATCGATGACTCTAAACTAGCCAGTGCAGATGATATTGGTACTTTGATCTGTAAGAACAAA
  AAACCGCTCATACAGGAGGAAAGTGACACCATTGTTTCTTCTTCCAAGAGTGCTTTACAT
  TCATCAGTGCCTAAAAGTACCAATGACAGAGATGCCACACCTCTGTCAAGAGCAATGGAC
  TTTGAAGGAAAACTGGGATGTGACTCTGAATCTAATAGCACTTTGGAAAATAGTTCTGAT
  ACCGTGTCTATTCAGGATAGCAGTGAAGAAGATATGATTGTTCAGAATAGCAATGAAAGC
  ATTTCTGAACAGTTCAGAACTCGAGAACAAGATGTTGAAGTCTTGGAGCCGTTAAAGTGT
  GAGTTGGTTTCTGGTGAGTCCACTGGAAACTGTGAGGACAGGCTGCCGGTCAAGGGGACT
  GAAGCAAATGGTAAAAAACCAAGTCAGCAGAAGAAATTAGAGGAGAGACCAGTTAATAAA
  TGTAGTGATCAAATAAAGCTAAAAAATACCACTGACAAAAAGAATAATGAAAATCGAGAG
  TCTGAAAAGAAAGGACAGAGAACAAGTACATTTCAAATAAATGGAAAAGATAATAAACCC
  AAAATATATTTGAAAGGTGAATGCTTGAAAGAAATTTCTGAGAGTAGAGTAGTAAGTGGT
  AATGTTGAACCAAAGGTTAATAATATAAATAAAATAATCCCTGAGAATGATATTAAATCA
  TTGACTGTTAAAGAATCTGCTATAAGGCCATTCATTAATGGTGATGTCATCATGGAAGAT
  TTTAATGAAAGAAACAGCTCCGAAACAAAATCGCATTTGCTGAGTTCTTCAGATGCTGAA
  GGTAACTACCGAGATAGCCTTGAGACCCTGCCATCAACCAAAGAGTCTGACAGTACACAG
  ACGACCACACCCTCAGCATCTTGTCCAGAAAGCAATTCAGTTAATCAGGTAGAAGATATG
  GAAATAGAAACCTCAGAAGTTAAGAAAGTTACTTCATCACCTATTACTTCTGAAGAGGAA
  TCTAATCTCAGTAATGACTTTATTGATGAAAATGGTCTGCCCATCAACAAAAATGAAAAT
  GTCAATGGAGAATCTAAAAGAAAAACCGTCATCACAGAAGTCACCACGATGACCTCCACA
  GTGGCCACAGAATCAAAAACTGTGATCAAGGTAGAAAAAGGCGATAAGCAAACTGTGGTT
  TCTTCCACAGAAAATTGTGCAAAATCCACTGTCACAACCACCACTACAACAGTGACCAAG
  CTTTCCACACCCTCCACAGGCGGCAGTGTGGACATCATCTCTGTAAAGGAGCAGAGCAAA
  ACCGTGGTCACCACGACAGTGACAGACTCCCTGACCACCACGGGAGGCACACTGGTTACA
  TCTATGACTGTGAGCAAAGAGTATTCCACACGAGACAAAGTGAAACTGATGAAATTTTCA
  AGACCAAAGAAGACTCGTTCAGGTACAGCTCTGCCATCCTATAGAAAATTTGTTACCAAG
  AGCAGCAAGAAGAGCATTTTTGTTTTGCCTAATGATGACTTAAAAAAGTTGGCCCGAAAA
  GGAGGAATCCGAGAGGTCCCTTATTTTAATTACAATGCAAAACCTGCTTTGGATATATGG
  CCATATCCTTCTCCTAGACCGACCTTTGGCATCACTTGGAGGTATAGACTTCAGACAGTA
  AAGTCCTTAGCTGGAGTGAGCCTGATGTTACGGTTACTGTGGGCAAGTTTGAGATGGGAT
  GATATGGCGGCCAAGGCTCCTCCAGGAGGAGGGACTACACGGACAGAAACATCCGAAACT
  GAAATCACAACAACAGAAATAATTAAGAGGAGAGATGTTGGTCCTTATGGCATTCGATCT
  GAATATTGTATCAGGAAAATCATTTGTCCCATTGGAGTTCCAGAAACACCAAAAGAAACG
  CCTACACCTCAGAGGAAAGGCCTTCGATCAAGTGCACTGCGGCCAAAGAGACCAGAAACG
  CCCAAGCAAACTGGCCCTGTTATTATTGAAACCTGGGTAGCAGAAGAAGAACTGGAATTG
  TGGGAGATCAGGGCATTTGCTGAGAGAGTGGAGAAAGAAAAGGCACAAGCAGTTGAGCAA
  CAGGCTAAGAAACGACTGGAGCAGCAGAAGCCGACAGTGATTGCAACTTCCACTACTTCC
  CCAACAAGCAGTACAACCAGCACCATCTCTCCAGCACAGAAGGTTATGGTGGCCCCCATA
  AGTGGCTCAGTTACAACTGGAACCAAAATGGTACTAACTACTAAAGTTGGATCTCCAGCT
  ACAGTAACATTCCAACAAAACAAGAACTTTCATCAAACCTTTGCTACATGGGTTAAGCAA
  GGCCAGTCAAATTCAGGCGTTGTTCAAGTACAGCAGAAAGTCCTGGGTATCATTCCATCA
  AGTACAGGTACCAGTCAGCAAACCTTTACTTCATTCCAGCCCAGGACAGCAACAGTCACA
  ATTAGGCCCAATACCTCAGGCTCTGGAGGAACCACAAGCAATTCACAAGTAATCACAGGG
  CCTCAGATTCGCCCTGGTATGACCGTGATTAGAACACCACTCCAACAGTCAACACTAGGA
  AAGGCAATTATTCGAACACCTGTGATGGTACAGCCAGGTGCTCCTCAGCAAGTGATGACT
  CAAATCATCAGGGGGCAGCCTGTCTCCACTGCAGTCTCCGCCCCTAACACGGTTTCCTCA
  ACACCTGGGCAGAAAAGCTTAACTTCAGCAACGTCCACTTCAAATATACAGTCTTCAGCC
  TCACAACCCCCTCGCCCCCAACAAGGACAAGTGAAGCTCACCATGGCTCAACTTACTCAG
  TTAACACAGGGCCACGGTGGCAATCAAGGTTTGACAGTAGTAATTCAAGGACAAGGTCAA
  ACTACTGGACAGTTGCAGTTGATACCTCAAGGGGTGACTGTACTCCCAGGCCCAGGCCAG
  CAGCTAATGCAAGCTGCAATGCCAAATGGTACTGTTCAGCGATTCCTCTTTACCCCATTG
  GCAACAACAGCCACCACAGCCAGCACCACCACCACCACTGTTTCCACGACAGCAGCAGGT
  ACAGGTGAACAAAGGCAGAGTAAACTGTCACCCCAGATGCAGGTACATCAAGACAAAACC
  CTGCCACCAGCTCAGTCATCAAGTGTGGGTCCAGCAGAAGCCCAGCCACAGACTGCTCAG
  CCTTCAGCTCAGCCCCAGCCCCAAACCCAGCCCCAGTCCCCAGCTCAGCCTGAAGTTCAG
  ACTCAGCCTGAAGTTCAGACCCAAACAACTGTTTCATCCCATGTCCCTTCTGAAGCACAA
  CCCACCCACGCACAGTCATCCAAGCCCCAAGTTGCAGCACAGTCTCAGCCTCAAAGTAAT
  GTCCAAGGACAGTCTCCTGTTCGTGTCCAAAGTCCATCACAGACTCGAATACGTCCATCA
  ACTCCATCCCAACTGTCTCCTGGACAACAATCCCAGGTTCAGACTACAACCTCACAACCG
  ATTCCAATTCAACCACATACATCTCTTCAGATACCTTCCCAAGGCCAGCCACAGTCACAA
  CCCCAGGTGGTGATGAAGCATAATGCTGTAATAGAACATTTAAAACAGAAAAAGAGCATG
  ACTCCAGCTGAAAGAGAAGAGAATCAAAGAATGATTGTCTGTAACCAGGTGATGAAGTAT
  ATTTTGGATAAGATAGATAAAGAAGAAAAACAGGCAGCAAAAAAACGGAAGCGTGAAGAG
  AGTGTGGAGCAGAAACGTAGCAAGCAGAATGCCACTAAGCTGTCAGCTCTGCTCTTCAAG
  CACAAAGAGCAGCTCAGAGCCGAGATCCTGAAGAAGAGAGCACTCCTGGACAAGGATCTG
  CAAATTGAAGTGCAGGAAGAGCTGAAGAGAGACCTGAAAATTAAGAAAGAAAAAGACCTG
  ATGCAGTTGGCTCAGGCCACAGCAGTAGCTGCACCCTGCCCCCCAGTGACACCAGCTCCT
  CCAGCCCCTCCAGCCCCTCCACCTTCACCTCCCCCTCCACCTGCTGTGCAACACACAGGC
  CTTCTGTCCACGCCCACCTTACCTGCTGCTTCCCAGAAGAGGAAGCGGGAAGAGGAAAAA
  GACTCCAGCTCAAAGTCCAAGAAAAAGAAAATGATCTCTACTACCTCAAAGGAAACTAAG
  AAGGACACAAAGCTTTACTGTATCTGTAAAACGCCTTATGATGAATCTAAATTTTATATT
  GGCTGTGATCGGTGTCAGAATTGGTACCATGGGCGCTGCGTTGGCATCTTGCAAAGTGAG
  GCAGAGCTCATTGATGAGTATGTCTGTCCACAGTGCCAGTCAACAGAGGATGCCATGACA
  GTGCTCACGCCACTAACAGAGAAGGATTATGAGGGGTTGAAGAGGGTGCTCCGTTCCTTA
  CAGGCCCATAAGATGGCCTGGCCTTTCCTTGAACCAGTAGACCCTAATGATGCACCAGAT
  TATTATGGTGTTATTAAGGAACCTATGGACCTTGCCACCATGGAAGAAAGAGTACAAAGA
  CGATATTATGAAAAGCTGACGGAATTTGTGGCAGATATGACCAAAATTTTTGATAACTGT
  CGTTACTACAATCCAAGTGACTCCCCATTTTACCAGTGTGCAGAAGTTCTCGAATCATTC
  TTTGTACAGAAATTGAAAGGCTTCAAAGCTAGCAGGTCTCATAACAACAAACTGCAGTCT
  ACAGCTTCTTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_004459
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004459.5, NP_004450.3
• RefSeq Size: 10884 bp
• RefSeq ORF: 10863 bp
• Locus ID: 2186
• Cytogenetics: 17q24.2
• Domains: PHD, DDT
• Protein Families: Druggable Genome
• Gene Summary: 'This gene was identified by the reactivity of its encoded protein to a monoclonal antibody prepared against brain homogenates from patients with Alzheimer's disease. Analysis of the original protein (fetal Alz-50 reactive clone 1, or FAC1), identified as an 810 aa protein containing a DNA-binding domain and a zinc finger motif, suggested it might play a role in the regulation of transcription. High levels of FAC1 were detected in fetal brain and in patients with neurodegenerative diseases. The protein encoded by this gene is actually much larger than originally thought, and it also contains a C-terminal bromodomain characteristic of proteins that regulate transcription during proliferation. The encoded protein is highly similar to the largest subunit of the Drosophila NURF (nucleosome remodeling factor) complex. In Drosophila, the NURF complex, which catalyzes nucleosome sliding on DNA and interacts with sequence-specific transcription factors, is necessary for the chromatin remodeling required for transcription. Two alternative transcripts encoding different isoforms have been described completely. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) contains two additional in-frame exons in the 5' coding region and uses an alternate in-frame splice site in the 3' coding region, compared to variant 1. The encoded isoform (2) is shorter than isoform 1.