MSH5 (NM_172166) Human Untagged Clone

CAT#: SC309519

MSH5 (untagged)-Human mutS homolog 5 (E. coli) (MSH5), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MSH5
• Synonyms: G7; MUTSH5; NG23; POF13
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_172166, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCTCCTTAGGAGCGAACCCAAGGAGGACACCGCAGGGACCGAGACCTGGGGCGGCCTCCTCCGGCT
  TCCCCAGCCCGGCCCCAGTGCCGGGCCCCAGGGAGGCCGAGGAGGAGGAAGTCGAGGAGGAGGAGGAGCT
  GGCCGAGATCCATCTGTGTGTGCTGTGGAATTCAGGATACTTGGGCATTGCCTACTATGATACTAGTGAC
  TCCACTATCCACTTCATGCCAGATGCCCCAGACCACGAGAGCCTCAAGCTTCTCCAGAGAGTTCTGGATG
  AGATCAATCCCCAGTCTGTTGTTACGAGTGCCAAACAGGATGAGAATATGACTCGATTTCTGGGAAAGCT
  TGCCTCCCAGGAGCACAGAGAGCCTAAAAGACCTGAAATCATATTTTTGCCAAGTGTGGATTTTGGTCTG
  GAGATAAGCAAACAACGCCTCCTTTCTGGAAACTACTCCTTCATCCCAGACGCCATGACTGCCACTGAGA
  AAATCCTCTTCCTCTCTTCCATTATTCCCTTTGACTGCCTCCTCACAGTTCGAGCACTTGGAGGGCTGCT
  GAAGTTCCTGGGTCGAAGAAGAATCGGGGTTGAACTGGAAGACTATAATGTCAGCGTCCCCATCCTGGGC
  TTTAAGAAATTTATGTTGACTCATCTGGTGAACATAGATCAAGACACTTACAGTGTTCTACAGATTTTTA
  AGAGTGAGTCTCACCCCTCAGTGTACAAAGTGGCCAGTGGACTGAAGGAGGGGCTCAGCCTCTTTGGAAT
  CCTCAACAGATGCCACTGTAAGTGGGGAGAGAAGCTGCTCAGGCTATGGTTCACACGTCCGACTCATGAC
  CTGGGGGAGCTCAGTTCTCGTCTGGACGTCATTCAGTTTTTTCTGCTGCCCCAGAATCTGGACATGGCTC
  AGATGCTGCATCGGCTCCTGGGTCACATCAAGAACGTGCCTCTGATTCTGAAACGCATGAAGTTGTCCCA
  CACCAAGGTCAGCGACTGGCAGGTTCTCTACAAGACTGTGTACAGTGCCCTGGGCCTGAGGGATGCCTGC
  CGCTCCCTGCCGCAGTCCATCCAGCTCTTTCGGGACATTGCCCAAGAGTTCTCTGATGACCTGCACCATA
  TCGCCAGCCTCATTGGGAAAGTAGTGGACTTTGAGGGCAGCCTTGCTGAAAATCGCTTCACAGTCCTCCC
  CAACATAGATCCTGAAATTGATGAGAAAAAGCGAAGACTGATGGGACTTCCCAGTTTCCTTACTGAGGTT
  GCCCGCAAGGAGCTGGAGAATCTGGACTCCCGTATTCCTTCATGCAGTGTCATCTACATCCCTCTGATTG
  GCTTCCTTCTTTCTATTCCCCGCCTGCCTTCCATGGTAGAGGCCAGTGACTTTGAGATTAATGGACTGGA
  CTTCATGTTTCTCTCAGAGGAGAAGCTGCACTATCGTAGTGCCCGAACCAAGGAGCTGGATGCATTGCTG
  GGGGACCTGCACTGCGAGATCCGGGACCAGGAGACGCTGCTGATGTACCAGCTACAGTGCCAGGTGCTGG
  CACGAGCAGCTGTCTTAACCCGAGTATTGGACCTTGCCTCCCGCCTGGACGTCCTGCTGGCTCTTGCCAG
  TGCTGCCCGGGACTATGGCTACTCAAGGCCGCGTTACTCCCCACAAGTCCTTGGGGTACGAATCCAGAAT
  GGCAGACATCCTCTGATGGAACTCTGTGCCCGAACCTTTGTGCCCAACTCCACAGAATGTGGTGGGGACA
  AAGGGAGGGTCAAAGTCATCACTGGACCCAACTCATCAGGGAAGAGCATATACCTCAAACAGGTAGGCTT
  GATCACATTCATGGCCCTGGTAGGCAGCTTTGTGCCAGCAGAGGAGGCCGAAATTGGGGCAGTAGACGCC
  ATCTTCACACGAATTCATAGCTGCGAATCCATCTCCCTTGGCCTCTCCACCTTCATGATCGACCTCAACC
  AGGTGGCGAAAGCAGTGAACAATGCCACTGCACAGTCGCTGGTCCTTATTGATGAATTTGGAAAGGGAAC
  CAACACGGTGGATGGGCTCGCGCTTCTGGCCGCTGTGCTCCGACACTGGCTGGCACGTGGACCCACATGC
  CCCCACATCTTTGTGGCCACCAACTTTCTGAGCCTTGTTCAGCTACAACTGCTGCCACAAGGGCCCCTGG
  TGCAGTATTTGACCATGGAGACCTGTGAGGATGGCAACGATCTTGTCTTCTTCTATCAGGTTTGCGAAGG
  TGTTGCGAAGGCCAGCCATGCCTCCCACACAGCTGCCCAGGCTGGGCTTCCTGACAAGCTTGTGGCTCGT
  GGCAAGGAGGTCTCAGACTTGATCCGCAGTGGAAAACCCATCAAGCCTGTCAAGGATTTGCTAAAGAAGA
  ACCAAATGGAAAATTGCCAGACATTAGTGGATAAGTTTATGAAACTGGATTTGGAAGATCCTAACCTGGA
  CTTGAACGTTTTCATGAGCCAGGAAGTGCTGCCTGCTGCCACCAGCATCCTCTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_172166
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172166.3, NP_751898.1
• RefSeq Size: 2787 bp
• RefSeq ORF: 2505 bp
• Locus ID: 4439
• Cytogenetics: 6p21.33
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the mutS family of proteins that are involved in DNA mismatch repair and meiotic recombination. This protein is similar to a Saccharomyces cerevisiae protein that participates in segregation fidelity and crossing-over events during meiosis. This protein plays a role in promoting ionizing radiation-induced apoptosis. This protein forms hetero-oligomers with another member of this family, mutS homolog 4. Polymorphisms in this gene have been linked to various human diseases, including IgA deficiency, common variable immunodeficiency, and premature ovarian failure. Alternative splicing results multiple transcript variants. Read-through transcription also exists between this gene and the downstream chromosome 6 open reading frame 26 (C6orf26) gene. [provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (4) uses alternate in-frame splice sites in both the central and 3' coding regions, compared to variant 1, resulting in an isoform (c) that is longer than isoform a. Both variants 3 and 4 encode the same isoform.