IRF5 (NM_002200) Human Untagged Clone

CAT#: SC309604

IRF5 (untagged)-Human interferon regulatory factor 5 (IRF5), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IRF5
• Synonyms: interferon regulatory factor 5; SLEB10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002200 edited
  GGTGCTCCCTGGCGCAGCCACGCAGGCGCACCGCAGACAGACCCCTCTGCCATGAACCAG
  TCCATCCCAGTGGCTCCCACCCCACCCCGCCGCGTGCGGCTGAAGCCCTGGCTGGTGGCC
  CAGGTGAACAGCTGCCAGTACCCAGGGCTTCAATGGGTCAACGGGGAAAAGAAATTATTC
  TGCATCCCCTGGAGGCATGCCACAAGGCATGGTCCCAGCCAGGACGGAGATAACACCATC
  TTCAAGGCCTGGGCCAAGGAGACAGGGAAATACACCGAAGGCGTGGATGAAGCCGATCCG
  GCCAAGTGGAAGGCCAACCTGCGCTGTGCCCTTAACAAGAGCCGGGACTTCCGCCTCATC
  TACGACGGGCCCCGGGACATGCCACCTCAGCCCTACAAGATCTACGAGGTCTGCTCCAAT
  GGCCCTGCTCCCACAGACTCCCAGCCCCCTGAGGATTACTCTTTTGGTGCAGGAGAGGAG
  GAGGAAGAAGAGGAAGAGCTGCAGAGGATGTTGCCAAGCCTGAGCCTCACAGATGCAGTG
  CAGTCTGGCCCCCACATGACACCCTATTCTTTACTCAAAGAGGATGTCAAGTGGCCGCCT
  ACTCTGCAGCCGCCCACTCTGCAGCCGCCCGTGGTGCTGGGTCCCCCTGCTCCAGACCCC
  AGCCCCCTGGCTCCTCCCCCTGGCAACCCTGCTGGCTTCAGGGAGCTTCTCTCTGAGGTC
  CTGGAGCCTGGGCCCCTGCCTGCCAGCCTGCCCCCTGCAGGCGAACAGCTCCTGCCAGAC
  CTGCTGATCAGCCCCCACATGCTGCCTCTGACCGACCTGGAGATCAAGTTTCAGTACCGG
  GGGCGGCCACCCCGGGCCCTCACCATCAGCAACCCCCATGGCTGCCGGCTCTTCTACAGC
  CAGCTGGAGGCCACCCAGGAGCAGGTGGAACTCTTCGGCCCCATAAGCCTGGAGCAAGTG
  CGCTTCCCCAGCCCTGAGGACATCCCCAGTGACAAGCAGCGCTTCTACACGAACCAGCTG
  CTGGATGTCCTGGACCGCGGGCTCATCCTCCAGCTACAGGGCCAGGACCTTTATGCCATC
  CGCCTGTGTCAGTGCAAGGTGTTCTGGAGCGGGCCTTGTGCCTCAGCCCATGACTCATGC
  CCCAACCCCATCCAGCGGGAGGTCAAGACCAAGCTTTTCAGCCTGGAGCATTTTCTCAAT
  GAGCTCATCCTGTTCCAAAAGGGCCAGACCAACACCCCACCACCCTTCGAGATCTTCTTC
  TGCTTTGGGGAAGAATGGCCTGACCGCAAACCCCGAGAGAAGAAGCTCATTACTGTACAG
  GTGGTGCCTGTAGCAGCTCGACTGCTGCTGGAGATGTTCTCAGGGGAGCTATCTTGGTCA
  GCTGATAGTATCCGGCTACAGATCTCAAACCCAGACCTCAAAGACCGCATGGTGGAGCAA
  TTCAAGGAGCTCCATCACATCTGGCAGTCCCAGCAGCGGTTGCAGCCTGTGGCCCAGGCC
  CCTCCTGGAGCAGGCCTTGGTGTTGGCCAGGGGCCCTGGCCTATGCACCCAGCTGGCATG
  CAATAACAAGGCTGCAGACGGTGACTGGCCCTGGCTTCCTGGGTGGCGGTGCGGACTGAT
  GTGGAGATGTGACAGCCCCGATGAGCACCTGGCTGGCTGCAGGGTCCTACCTCTGGGTTT
  CCTGGAAGTGGATTTGGGCCAAGAAGGAGAGGGAGAAAGGCCCGAGCCCCTGCCTTCCCG
  GGCCTTTCTCTCCTGGGCTGTCTCTGGTCTGGTCAGCCTGGCTCTCGGGAAATTCAGCCA
  TGAGCAGGGAAAGAACTCTCCCAACCCTGGGGCCTAGCTGTATAGGAGGAATTGCCTAAG
  GGTGGCCCACTCTTGTGATTGCCCCATTTCCTCTGGCAACAAAAGCCAGAGTGTTGTGGG
  CCAAGTCCCCCCACAGGGCCTCTGCAGGGCATGGCCCTGATTTCCCTGGTTTGAGACTCA
  CTTCCTCATCTCCCTGTCCTCTGAGATAATATGAGTGAGCACTTAGGTATCATATCAGAT
  GCTCAAGGCTGGCAGCTACCCCCTTCTTGAGAGTCCAAGAACCTGGAGCAGAAATAATTT
  TTATGTATTTTTGGATTAATGAATGTTAAAAACAGACTCAGCTGTTTCTTTCCTTTTACT
  ACTACCAGTTGCTCCCATGCTGCTCCACCAGGCCCTGTTTCGGATGCCAACTGGCCCACT
  CCCCAAGCACTTGCCCCCAGCTTGCGACCATTGGCACTGGGAGGGCCTGGCTTCTGGGCT
  GATGGGTCAGTTGGGCCTTCATAAACACTCACCTGGCTGGCTTTGCCTTCCAGGAGGAAG
  CTGGCTGAAGCAAGGGTGTGGAATTTTAAATGTGTGCACAGTCTGGAAAACTGTCAGAAT
  CAGTTTTCCCATAAAAGGGTGGGCTAGCATTGCAGCTGCATTTGGGACCATTCAAATCTG
  TCACTCTCTTGTGTATATTCCTGTGCTATTAAATATATCAGGGCAGTGCATGTAAATCAT
  CCTGATATATTTAATATATTTATTATATTGTCCCCCGAGGTGGGGACAGTGAGTGAGTTC
  TCTTAGTCCCCCCAGAGCTGGTTGTTAAAGAGCCTGGCACCTACCCGCTCTCACTTCATC
  TGTGTCATCTCTGCACACTCCAGCCCACTTTCTGCCTTCAGCCATTGAGTGGAAGCTGCC
  CCAGGCCCTTACCAGGTGCAGATGCCCAATCTTGATGCCCAGCCATCAGAACTGTGAGCC
  AAATAAACCTTTTTCTGTATAAATTAAAAAAAAAAAAAAAAAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_002200 unedited
  CCCCCATGGNGATGGCAACTTCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGNATGC
  CCCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTT
  TTTTTTTTTTTTAATTTATACAGAAAAAAGGTTTATNNTTGGCTCACAGTTCTGATGGCT
  GGGCATCAAGATTGGGCATCTGCACCTGGTAAGGGCCTGGGGCAGCTTCCACTCAATGGC
  TGAAGGCAGAAAGTGGGCTGGAGTGTGCAGAGATGACACAGATGAAGTGAGAGCGGGTAG
  GTGCCAGGCTCTTTAACAACCAGCTCTGGGGGGACTAAGAGAACTCACTCACTGTCCCCA
  CCTCGGGGGACAATATAATAAATATATTAAATATATCAGGATGATTTACATGCACTGCCC
  TGATATATTTAATAGCACAGGAATATACACAAGAGAGTGACAGATTTGAATGGTCCCAAA
  TGCAGCTGCAATGCTAGCCCACCCTTTTATGGGAAAACTGATTCTGACAGTTTTCCAGAC
  TGTGCACACATTTAAAATTCCACACCCTTGCTTCAGCCAGCTTCCTCCTGGAAGGCAAAG
  CCAGCCAGGTGAGTGTTTATGAAGGCCCAACTGACCCATCAGCCCAGAAGCCAGGCCCTC
  CCAGTGCCAATGGTCGCAAGCTGGGGGCAAGTGCTTGGGGAGTGGGCCAGTTGGCATCCG
  ANACAGGGCCTGGTGGAGCAGCATGGGAGCAACTGGTAGTAGTAAAAGGAAAGAAACAGC
  TGAGTCTGTTTTAACATTCATTAATCCAAAATACATANAATTATTTCTGGCTCCAGTTCT
  TGGACTCTCAGGAAGGGGTAGCTGCCAACCTTGACATCTGATTGATACCTAGTGCTCC
  
• Restriction Sites: Please inquire
• ACCN: NM_002200
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002200.3, NP_002191.1
• RefSeq Size: 2870 bp
• RefSeq ORF: 1515 bp
• Locus ID: 3663
• Cytogenetics: 7q32.1
• Protein Families: Transcription Factors
• Protein Pathways: Toll-like receptor signaling pathway
• Gene Summary: 'This gene encodes a member of the interferon regulatory factor (IRF) family, a group of transcription factors with diverse roles, including virus-mediated activation of interferon, and modulation of cell growth, differentiation, apoptosis, and immune system activity. Members of the IRF family are characterized by a conserved N-terminal DNA-binding domain containing tryptophan (W) repeats. Alternative promoter use and alternative splicing result in multiple transcript variants, and a 30-nt indel polymorphism (SNP rs60344245) can result in loss of a 10-aa segment. [provided by RefSeq, Dec 2016]'
  Transcript Variant: This variant (1) lacks an alternate in-frame segment compared to variant 5. The resulting isoform (a) has the same N- and C-termini but is shorter compared to isoform d.