Cytochrome b5 (CYB5A) (NM_148923) Human Untagged Clone

CAT#: SC309790

CYB5A (untagged)-Human cytochrome b5 type A (microsomal) (CYB5A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CYB5A
• Synonyms: CYB5; MCB5; METAG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_148923 edited
  CCTCGTGCCGAATTCGGCACGAGGGGGGAATGTCCCGGGTGGAGCTGGCTGAGTCGCGCG
  CTCTGCTCCACCCGACGGGGCTGTGTGTGCTGGGCCTGGCTCGCGGCGAACCGAGATGGC
  AGAGCAGTCGGACGAGGCCGTGAAGTACTACACCCTAGAGGAGATTCAGAAGCACAACCA
  CAGCAAGAGCACCTGGCTGATCCTGCACCACAAGGTGTACGATTTGACCAAATTTCTGGA
  AGAGCATCCTGGTGGGGAAGAAGTTTTAAGGGAACAAGCTGGAGGTGACGCTACTGAGAA
  CTTTGAGGATGTCGGGCACTCTACAGATGCCAGGGAAATGTCCAAAACATTCATCATTGG
  GGAGCTCCATCCAGATGACAGACCAAAGTTAAACAAGCCTCCGGAAACTCTTATCACTAC
  TATTGATTCTAGTTCCAGTTGGTGGACCAACTGGGTGATCCCTGCCATCTCTGCAGTGGC
  CGTCGCCTTGATGTATCGCCTATACATGGCAGAGGACTGAACACCTCCTCAGAAGTCAGC
  GCAGGAAGAGCCTGCTTTGGACACGGGAGAAAAGAAGCCATTGCTAACTACTTCAACTGA
  CAGAAACCTTCACTTGAAAACAATGATTTTAATATATCTCTTTCTTTTTCTTCCGACATT
  AGAAACAAAACAAAAAGAACTGTCCTTTCTGCGCTCAAATTTTTCGAGTGTGCCTTTTTA
  TTCATCTACTTTATTTTGATGTTTCCTTAATGTGTAATTTACTTATTATAAGCATGATCT
  TTTAAAAATATATTTGGCTTTTAAAGTAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_148923 unedited
  ACGACTCATATAGGGCGGCCGCGATTCGGCACGAGGCCTCGTGCCGAATTCGGCACGAGG
  GGGGAATGTCCCGGGTGGAGCTGGCTGAGTCGCGCGCTCTGCTCCACCCGACGGGGCTGT
  GTGTGCTGGGCCTGGCTCGCGGCGAACCGAGATGGCAGAGCAGTCGGACGAGGCCGTGAA
  GTACTACACCCTAGAGGAGATTCAGAAGCACAACCACAGCAAGAGCACCTGGCTGATCCT
  GCACCACAAGGTGTACGATTTGACCAAATTTCTGGAAGAGCATCCTGGTGGGGAAGAAGT
  TTTAAGGGAACAAGCTGGAGGTGACGCTACTGAGAACTTTGAGGATGTCGGGCACTCTAC
  AGATGCCAGGGAAATGTCCAAAACATTCATCATTGGGGAGCTCCATCCAGATGACAGACC
  AAAGTTAAACAAGCCTCCGGAAACTCTTATCACTACTATTGATTCTAGTTCCAGTTGGTG
  GACCAACTGGGTGATCCCTGCCATCTCTGCAGTGGCCGTCGCCTTGATGTATCGCCTATA
  CATGGCAGAGGACTGAACACCTCCTCAGAAGTCAGCGCAGGAAGAGCCTGCTTTGGACAC
  GGGAGAAAAGAAGCCATTGCTAACTACTTCAACTGACAGAAACCTTCACTTGAAAACAAT
  GATTNTAATATATCTCTTTCTTTNTCTTCCGACATTAGAAACAAAACAAAAAGAACTGTC
  CTTTCTGCGCTCAAATTTTTCGAGTGTGCCTTTTTATTCATCTACTTTATTNTGATGGTT
  CCTTTATGGNNGTATTACTTTTATATAGCATGATCTTTTAAAATATATTTGGCTTTTAAG
  TANAAAAAAAAAAAAACTCGCTCTAGATTGGGNCGCGTCATAGCTGTTTCCTGACAGACC
  GGNNTGCATCCCTGTGACCCTNCCCAGTGCTTCCTGGCCTGNNAGTGC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_148923 unedited
  CCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTACTTTAAAAGCCAAATATATTTTT
  AAAAGATCATGCTTATAATAAGTAAATTACACATTAAGGAAACATCAAAATAAAGTAGAT
  GAATAAAAAGGCACACTTCGAAAAATTTGAGCGCAGAAAGGACAGTTCTTTTTGTTTTGT
  TTCTAATGTCGGAAGAAAAAGAAAGAGATATATTAAAATCATTGTTTTCAAGTGAAGGTT
  TCTGTCAGTTGAAGTAGTTAGCAATGGCTTCTTTTCTCCCGTGTCCAAAGCAGGCTCTTC
  CTGCGCTGACTTCTGAGGAGGTGTTCAGTCCTCTGCCATGTATAGGCGATACATCAAGGC
  GACGGCCACTGCAGAGATGGCAGGGATCACCCAGTTGGTCCACCAACTGGAACTAGAATC
  AATAGTAGTGATAAGAGTTTCCGGAGGCTTGTTTAACTTTGGTCTGTCATCTGGATGGAG
  CTCCCCAATGATGAATGTTTTGGACATTTCCCTGGCATCTGTAGAGTGCCCGACATCCTC
  AAAGTTCTCAGTAGCGTCACCTCCAGCTTGTTCCCTTAAAACTTCTTCCCCACCAGGATG
  CTCTTCCAGAAATTTGGTCAAATCGTACACCTTGTGGTGCANGATCAGCCAGGTGCTCTT
  GCTGTGGTTGTGCTTCTGAAATCTCTCTAGGNTGTAGTACTTCACGGNCTCGTNCGACTG
  CTCTTGCATCTCGGGGTTCGCGCGAGCCANGCCCCAGACACACAGCCCNGTNNNGGTGGG
  GAGCANAAGGGCGCGACTTAACCAGNTTTCCACCCGGGACATTCCCCCTCGTGCCGAATT
  CGGGNCGAGGGCTTGTGCCGAATTCCGGGGCGCCCTTAAGGGGATTCGTATTACAAATTT
  TGGACGGTCACTAAACAAACTTGCTAAAATTAACTTTCACGGTACAGCCTACCGCCCATT
  GGTAACGGGGGGGGGGGTATATACAAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_148923
• Insert Size: 900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148923.2, NP_683725.1
• RefSeq Size: 820 bp
• RefSeq ORF: 405 bp
• Locus ID: 1528
• Cytogenetics: 18q22.3
• Protein Families: Transmembrane
• Gene Summary: 'The protein encoded by this gene is a membrane-bound cytochrome that reduces ferric hemoglobin (methemoglobin) to ferrous hemoglobin, which is required for stearyl-CoA-desaturase activity. Defects in this gene are a cause of type IV hereditary methemoglobinemia. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2010]'
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1).