DNMT1 (NM_001379) Human Untagged Clone

CAT#: SC309900

DNMT1 (untagged)-Human DNA (cytosine-5-)-methyltransferase 1 (DNMT1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DNMT1
• Synonyms: ADCADN; AIM; CXXC9; DNMT; HSN1E; m.HsaI; MCMT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001379, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCCGGCGCGTACCGCCCCAGCCCGGGTGCCCACACTGGCCGTCCCGGCCATCTCGCTGCCCGACGATG
  TCCGCAGGCGGCTCAAAGATTTGGAAAGAGACAGCTTAACAGAAAAGGAATGTGTGAAGGAGAAATTGAA
  TCTCTTGCACGAATTTCTGCAAACAGAAATAAAGAATCAGTTATGTGACTTGGAAACCAAATTACGTAAA
  GAAGAATTATCCGAGGAGGGCTACCTGGCTAAAGTCAAATCCCTTTTAAATAAAGATTTGTCCTTGGAGA
  ACGGTGCTCATGCTTACAACCGGGAAGTGAATGGACGTCTAGAAAACGGGAACCAAGCAAGAAGTGAAGC
  CCGTAGAGTGGGAATGGCAGATGCCAACAGCCCCCCCAAACCCCTTTCCAAACCTCGCACGCCCAGGAGG
  AGCAAGTCCGATGGAGAGGCTAAGCCTGAACCTTCACCTAGCCCCAGGATTACAAGGAAAAGCACCAGGC
  AAACCACCATCACATCTCATTTTGCAAAGGGCCCTGCCAAACGGAAACCTCAGGAAGAGTCTGAAAGAGC
  CAAATCGGATGAGTCCATCAAGGAAGAAGACAAAGACCAGGATGAGAAGAGACGTAGAGTTACATCCAGA
  GAACGAGTTGCTAGACCGCTTCCTGCAGAAGAACCTGAAAGAGCAAAATCAGGAACGCGCACTGAAAAGG
  AAGAAGAAAGAGATGAAAAAGAAGAAAAGAGACTCCGAAGTCAAACCAAAGAACCAACACCCAAACAGAA
  ACTGAAGGAGGAGCCGGACAGAGAAGCCAGGGCAGGCGTGCAGGCTGACGAGGACGAAGATGGAGACGAG
  AAAGATGAGAAGAAGCACAGAAGTCAACCCAAAGATCTAGCTGCCAAACGGAGGCCCGAAGAAAAAGAAC
  CTGAAAAAGTAAATCCACAGATTTCTGATGAAAAAGACGAGGATGAAAAGGAGGAGAAGAGACGCAAAAC
  GACCCCCAAAGAACCAACGGAGAAAAAAATGGCTCGCGCCAAAACAGTCATGAACTCCAAGACCCACCCT
  CCCAAGTGCATTCAGTGCGGGCAGTACCTGGACGACCCTGACCTCAAATATGGGCAGCACCCACCAGACG
  CGGTGGATGAGCCACAGATGCTGACAAATGAGAAGCTGTCCATCTTTGATGCCAACGAGTCTGGCTTTGA
  GAGTTATGAGGCGCTTCCCCAGCACAAACTGACCTGCTTCAGTGTGTACTGTAAGCACGGTCACCTGTGT
  CCCATCGACACCGGCCTCATCGAGAAGAATATCGAACTCTTCTTTTCTGGTTCAGCAAAACCAATCTATG
  ATGATGACCCATCTCTTGAAGGTGGTGTTAATGGCAAAAATCTTGGCCCCATAAATGAATGGTGGATCAC
  TGGCTTTGATGGAGGTGAAAAGGCCCTCATCGGCTTCAGCACCTCATTTGCCGAATACATTCTGATGGAT
  CCCAGTCCCGAGTATGCGCCCATATTTGGGCTGATGCAGGAGAAGATCTACATCAGCAAGATTGTGGTGG
  AGTTCCTGCAGAGCAATTCCGACTCGACCTATGAGGACCTGATCAACAAGATCGAGACCACGGTTCCTCC
  TTCTGGCCTCAACTTGAACCGCTTCACAGAGGACTCCCTCCTGCGACACGCGCAGTTTGTGGTGGAGCAG
  GTGGAGAGTTATGACGAGGCCGGGGACAGTGATGAGCAGCCCATCTTCCTGACACCCTGCATGCGGGACC
  TGATCAAGCTGGCTGGGGTCACGCTGGGACAGAGGCGAGCCCAGGCGAGGCGGCAGACCATCAGGCATTC
  TACCAGGGAGAAGGACAGGGGACCCACGAAAGCCACCACCACCAAGCTGGTCTACCAGATCTTCGATACT
  TTCTTCGCAGAGCAAATTGAAAAGGATGACAGAGAAGACAAGGAGAACGCCTTTAAGCGCCGGCGATGTG
  GCGTCTGTGAGGTGTGTCAGCAGCCTGAGTGTGGGAAATGTAAAGCCTGCAAGGACATGGTTAAATTTGG
  TGGCAGTGGACGGAGCAAGCAGGCTTGCCAAGAGCGGAGGTGTCCCAATATGGCCATGAAGGAGGCAGAT
  GACGATGAGGAAGTCGATGATAACATCCCAGAGATGCCGTCACCCAAAAAAATGCACCAGGGGAAGAAGA
  AGAAACAGAACAAGAATCGCATCTCTTGGGTCGGAGAAGCCGTCAAGACTGATGGGAAGAAGAGTTACTA
  TAAGAAGGTGTGCATTGATGCGGAAACCCTGGAAGTGGGGGACTGTGTCTCTGTTATTCCAGATGATTCC
  TCAAAACCGCTGTATCTAGCAAGGGTCACGGCGCTGTGGGAGGACAGCAGCAACGGGCAGATGTTTCACG
  CCCACTGGTTCTGCGCTGGGACAGACACAGTCCTCGGGGCCACGTCGGACCCTCTGGAGCTGTTCTTGGT
  GGATGAATGTGAGGACATGCAGCTTTCATATATCCACAGCAAAGTGAAAGTCATCTACAAAGCCCCCTCC
  GAAAACTGGGCCATGGAGGGAGGCATGGATCCCGAGTCCCTGCTGGAGGGGGACGACGGGAAGACCTACT
  TCTACCAGCTGTGGTATGATCAAGACTACGCGAGATTCGAGTCCCCTCCAAAAACCCAGCCAACAGAGGA
  CAACAAGTTCAAATTCTGTGTGAGCTGTGCCCGTCTGGCTGAGATGAGGCAAAAAGAAATCCCCAGGGTC
  CTGGAGCAGCTCGAGGACCTGGATAGCCGGGTCCTCTACTACTCAGCCACCAAGAACGGCATCCTGTACC
  GAGTTGGTGATGGTGTGTACCTGCCCCCTGAGGCCTTCACGTTCAACATCAAGCTGTCCAGTCCCGTGAA
  ACGCCCACGGAAGGAGCCCGTGGATGAGGACCTGTACCCAGAGCACTACCGGAAATACTCCGACTACATC
  AAAGGCAGCAACCTGGATGCCCCTGAGCCCTACCGAATTGGCCGGATCAAAGAGATCTTCTGTCCCAAGA
  AGAGCAACGGCAGGCCCAATGAGACTGACATCAAAATCCGGGTCAACAAGTTCTACAGGCCTGAGAACAC
  CCACAAGTCCACTCCAGCGAGCTACCACGCAGACATCAACCTGCTCTACTGGAGCGACGAGGAGGCCGTG
  GTGGACTTCAAGGCTGTGCAGGGCCGCTGCACCGTGGAGTATGGGGAGGACCTGCCCGAGTGCGTCCAGG
  TGTACTCCATGGGCGGCCCCAACCGCTTCTACTTCCTCGAGGCCTATAATGCAAAGAGCAAAAGCTTTGA
  AGATCCTCCCAACCATGCCCGTAGCCCTGGAAACAAAGGGAAGGGCAAGGGAAAAGGGAAGGGCAAGCCC
  AAGTCCCAAGCCTGTGAGCCGAGCGAGCCAGAGATAGAGATCAAGCTGCCCAAGCTGCGGACCCTGGATG
  TGTTTTCTGGCTGCGGGGGGTTGTCGGAGGGATTCCACCAAGCAGGCATCTCTGACACGCTGTGGGCCAT
  CGAGATGTGGGACCCTGCGGCCCAGGCGTTCCGGCTGAACAACCCCGGCTCCACAGTGTTCACAGAGGAC
  TGCAACATCCTGCTGAAGCTGGTCATGGCTGGGGAGACCACCAACTCCCGCGGCCAGCGGCTGCCCCAGA
  AGGGAGACGTGGAGATGCTGTGCGGCGGGCCGCCCTGCCAGGGCTTCAGCGGCATGAACCGCTTCAATTC
  GCGCACCTACTCCAAGTTCAAAAACTCTCTGGTGGTTTCCTTCCTCAGCTACTGCGACTACTACCGGCCC
  CGGTTCTTCCTCCTGGAGAATGTCAGGAACTTTGTCTCCTTCAAGCGCTCCATGGTCCTGAAGCTCACCC
  TCCGCTGCCTGGTCCGCATGGGCTATCAGTGCACCTTCGGCGTGCTGCAGGCCGGTCAGTACGGCGTGGC
  CCAGACTAGGAGGCGGGCCATCATCCTGGCCGCGGCCCCTGGAGAGAAGCTCCCTCTGTTCCCGGAGCCA
  CTGCACGTGTTTGCTCCCCGGGCCTGCCAGCTGAGCGTGGTGGTGGATGACAAGAAGTTTGTGAGCAACA
  TAACCAGGTTGAGCTCGGGTCCTTTCCGGACCATCACGGTGCGAGACACGATGTCCGACCTGCCGGAGGT
  GCGGAATGGAGCCTCGGCACTGGAGATCTCCTACAACGGGGAGCCTCAGTCCTGGTTCCAGAGGCAGCTC
  CGGGGCGCACAGTACCAGCCCATCCTCAGGGACCACATCTGTAAGGACATGAGTGCATTGGTGGCTGCCC
  GCATGCGGCACATCCCCTTGGCCCCAGGGTCAGACTGGCGCGATCTGCCCAACATCGAGGTGCGGCTCTC
  AGACGGCACCATGGCCAGGAAGCTGCGGTATACCCACCATGACAGGAAGAACGGCCGCAGCAGCTCTGGG
  GCCCTCCGTGGGGTCTGCTCCTGCGTGGAAGCCGGCAAAGCCTGCGACCCCGCAGCCAGGCAGTTCAACA
  CCCTCATCCCCTGGTGCCTGCCCCACACCGGGAACCGGCACAACCACTGGGCTGGCCTCTATGGAAGGCT
  CGAGTGGGACGGCTTCTTCAGCACAACCGTCACCAACCCCGAGCCCATGGGCAAGCAGGGCCGCGTGCTC
  CACCCAGAGCAGCACCGTGTGGTGAGCGTGCGGGAGTGTGCCCGCTCCCAGGGCTTCCCTGACACCTACC
  GGCTCTTCGGCAACATCCTGGACAAGCACCGGCAGGTGGGCAATGCCGTGCCACCGCCCCTGGCCAAAGC
  CATTGGCTTGGAGATCAAGCTTTGTATGTTGGCCAAAGCCCGAGAGAGTGCCTCAGCTAAAATAAAGGAG
  GAGGAAGCTGCTAAGGACTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001379
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001379.3, NP_001370.1
• RefSeq Size: 5422 bp
• RefSeq ORF: 4851 bp
• Locus ID: 1786
• Cytogenetics: 19p13.2
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Cysteine and methionine metabolism, Metabolic pathways
• Gene Summary: 'This gene encodes an enzyme that transfers methyl groups to cytosine nucleotides of genomic DNA. This protein is the major enzyme responsible for maintaining methylation patterns following DNA replication and shows a preference for hemi-methylated DNA. Methylation of DNA is an important component of mammalian epigenetic gene regulation. Aberrant methylation patterns are found in human tumors and associated with developmental abnormalities. Variation in this gene has been associated with cerebellar ataxia, deafness, and narcolepsy, and neuropathy, hereditary sensory, type IE. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (2, also known as Dnmt1a in PMIDs 10449766 and 10753866) lacks an alternate in-frame exon in the 5' coding region compared to variant 1. The resulting isoform (b) is shorter than isoform a.