TBC1D4 (NM_014832) Human Untagged Clone

CAT#: SC309926

TBC1D4 (untagged)-Human TBC1 domain family, member 4 (TBC1D4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBC1D4
• Synonyms: AS160; NIDDM5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014832, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCCGCCCAGCTGCATTCAGGATGAGCCGTTCCCGCACCCCCTGGAGCCCGAGCCGGGCGTCTCAG
  CTCAGCCCGGCCCCGGGAAGCCAAGCGATAAGCGGTTCCGGCTGTGGTACGTTGGGGGGTCGTGCCTGGA
  CCACAGGACCACGCTGCCTATGCTGCCCTGGCTCATGGCCGAGATCCGCAGGCGCAGCCAGAAGCCCGAG
  GCGGGCGGCTGCGGGGCGCCGGCGGCCCGAGAGGTGATCCTGGTGCTCAGCGCGCCCTTCCTGCGTTGCG
  TCCCCGCGCCGGGCGCTGGGGCCTCGGGGGGCACTAGTCCGTCGGCCACGCAGCCCAACCCGGCGGTATT
  CATCTTCGAGCACAAGGCGCAGCATATCTCGCGCTTCATCCACAACAGCCACGACCTCACCTACTTTGCC
  TACCTGATCAAGGCGCAGCCCGACGACCCCGAGTCGCAGATGGCCTGCCACGTTTTCCGCGCCACAGACC
  CCAGCCAGGTTCCTGATGTTATTAGCAGCATAAGGCAATTATCTAAAGCGGCCATGAAAGAGGATGCCAA
  ACCCAGCAAAGATAATGAGGACGCCTTTTACAACTCTCAGAAGTTCGAAGTCCTGTACTGTGGAAAGGTG
  ACCGTGACCCACAAGAAGGCCCCCTCAAGCCTCATCGATGACTGCATGGAGAAGTTCAGCCTGCACGAAC
  AGCAGCGCCTGAAGATCCAAGGGGAGCAGCGCGGTCCGGACCCAGGAGAGGACCTGGCTGACTTGGAGGT
  GGTGGTGCCCGGGTCCCCCGGAGACTGCCTGCCGGAGGAGGCTGACGGCACCGACACCCACCTTGGCTTA
  CCTGCCGGGGCCAGCCAGCCTGCCCTGACCAGCTCTCGGGTCTGCTTCCCTGAGCGGATTTTGGAAGATT
  CTGGCTTTGATGAGCAGCAGGAGTTTCGGTCTCGGTGCAGCAGTGTCACCGGCGTGCAACGGAGAGTTCA
  CGAGGGCAGCCAGAAATCCCAGCCGCGACGGAGACACGCGAGCGCACCCAGTCACGTCCAGCCCTCGGAC
  TCGGAGAAGAACAGGACCATGCTCTTCCAGGTTGGGCGATTTGAGATTAACCTTATCAGTCCAGACACTA
  AATCAGTTGTGCTAGAAAAGAATTTTAAAGATATCTCCTCTTGTTCTCAGGGTATAAAGCATGTGGATCA
  CTTTGGCTTTATCTGCCGGGAGTCTCCAGAGCCTGGACTTAGCCAGTATATTTGTTATGTATTCCAGTGT
  GCCAGCGAATCTCTGGTTGATGAGGTAATGCTGACTCTGAAACAGGCCTTCAGTACGGCGGCTGCCCTGC
  AGAGTGCCAAGACGCAGATTAAACTGTGTGAGGCCTGCCCGATGCACTCTTTGCATAAGCTCTGTGAAAG
  GATTGAAGGTCTCTACCCACCAAGAGCCAAGCTGGTGATACAGAGGCATCTCTCATCACTGACAGATAAT
  GAGCAAGCTGACATCTTTGAAAGAGTTCAGAAAATGAAGCCAGTCAGTGACCAGGAAGAAAATGAACTTG
  TGATTTTACACCTGAGGCAGCTGTGTGAAGCCAAGCAGAAGACACACGTGCACATCGGGGAAGGCCCTTC
  TACTATTTCAAATAGTACAATCCCAGAAAATGCAACAAGCAGTGGAAGGTTCAAACTTGACATTCTGAAA
  AATAAAGCTAAGAGATCCTTAACTAGCTCCCTGGAAAATATCTTCTCAAGGGGAGCTAACAGAATGAGAG
  GTCGGCTTGGAAGTGTGGACAGTTTTGAACGGTCCAACAGTCTTGCTTCAGAGAAGGACTACTCACCAGG
  GGATTCTCCACCAGGGACACCGCCAGCGTCCCCACCGTCCTCAGCTTGGCAAACGTTTCCCGAAGAGGAT
  TCCGACTCCCCGCAGTTTCGAAGACGGGCACACACGTTCAGCCACCCACCTTCAAGCACAAAGAGAAAGC
  TGAATTTGCAGGATGGGAGGGCTCAGGGTGTGCGTTCCCCTCTGCTGAGGCAGAGCTCCAGTGAACAGTG
  CAGCAATCTTTCGTCAGTTCGACGCATGTACAAGGAGAGTAATTCTTCCTCCAGTCTTCCAAGTCTTCAC
  ACTTCCTTCTCTGCCCCTTCCTTCACTGCCCCCTCTTTCCTGAAAAGCTTTTACCAGAATTCAGGTAGAC
  TGTCCCCACAGTATGAAAATGAAATCAGACAAGACACTGCTTCAGAATCAAGTGATGGAGAAGGGAGAAA
  AAGGACCTCATCTACCTGCAGCAATGAGTCCCTAAGTGTGGGAGGAACCTCTGTCACTCCTCGCCGGATC
  TCCTGGCGGCAGCGCATTTTCCTCAGGGTTGCTTCTCCCATGAACAAATCTCCCTCAGCAATGCAACAGC
  AAGATGGATTGGACAGGAACGAGCTGCTGCCACTGTCCCCCCTCTCTCCAACCATGGAGGAGGAACCGCT
  GGTTGTATTCCTGTCTGGGGAGGATGACCCAGAAAAGATTGAAGAAAGAAAGAAATCAAAAGAACTGAGG
  AGCTTGTGGAGAAAAGCTATACACCAACAAATCTTGTTACTTCGAATGGAAAAAGAAAACCAGAAACTTG
  AAGCAAGCAGAGATGAACTCCAGTCCAGAAAAGTTAAATTAGACTATGAAGAAGTTGGTGCATGTCAGAA
  AGAGGTCTTAATAACTTGGGATAAGAAGTTGTTAAACTGCAGAGCTAAAATCAGATGTGATATGGAAGAT
  ATTCATACTCTTCTTAAAGAAGGAGTTCCCAAAAGTCGACGAGGAGAAATTTGGCAGTTTCTGGCTTTAC
  AGTACCGACTCAGACACAGATTGCCTAATAAACAACAGCCTCCTGACATATCCTATAAGGAACTTTTGAA
  GCAGCTCACTGCTCAGCAGCATGCGATTCTCGTGGATTTAGGAAGGACGTTTCCTACTCACCCTTACTTT
  TCAGTACAGCTTGGGCCAGGACAGCTGTCACTGTTTAACCTCCTGAAAGCCTATTCTTTGCTGGACAAAG
  AAGTGGGATACTGTCAGGGGATCAGCTTTGTGGCTGGAGTCCTGCTTCTGCACATGAGTGAAGAGCAAGC
  CTTTGAAATGCTGAAATTCCTCATGTATGACCTCGGCTTCCGCAAGCAGTACAGACCTGACATGATGTCG
  CTGCAGATTCAAATGTACCAGCTGTCCAGGCTCCTTCATGACTATCACAGAGATCTCTACAATCACCTTG
  AAGAAAATGAAATCAGCCCCAGTCTTTATGCTGCCCCCTGGTTCCTCACATTGTTTGCCTCTCAGTTTTC
  ATTAGGATTTGTAGCCAGAGTTTTTGATATTATTTTTCTTCAGGGAACTGAAGTTATATTCAAGGTTGCA
  CTCAGCCTACTGAGCAGCCAAGAGACACTTATAATGGAATGTGAGAGCTTTGAAAATATTGTTGAGTTTC
  TTAAAAACACGCTACCTGATATGAATACCTCTGAAATGGAAAAAATTATTACCCAGGTTTTTGAGATGGA
  TATTTCTAAGCAGTTGCATGCCTATGAGGTGGAATATCATGTGCTACAGGATGAGCTTCAGGAATCTTCA
  TATTCCTGTGAGGATAGTGAAACTTTGGAGAAGCTGGAGAGGGCCAATAGCCAACTGAAAAGACAAAACA
  TGGACCTCCTAGAAAAATTACAGGTAGCTCATACTAAAATCCAGGCCTTGGAATCAAACCTGGAAAATCT
  TTTGACGAGAGAGACCAAAATGAAGTCTTTAATCCGGACCCTGGAACAAGAAAAAATGGCTTATCAAAAG
  ACAGTGGAGCAACTCCGGAAGCTGCTGCCCGCGGATGCTCTAGTCAATTGTGACCTGTTGCTGAGAGACC
  TAAACTGCAACCCTAACAACAAAGCCAAGATAGGAAATAAGCCATAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014832
• ORF Size: 3897 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_014832.4, NP_055647.2
• RefSeq Size: 6443
• RefSeq ORF: 3897
• Locus ID: 9882
• Domains: TBC, PID
• Gene Summary: This gene is a member of the Tre-2/BUB2/CDC16 domain family. The protein encoded by this gene is a Rab-GTPase-activating protein, and contains two phopshotyrosine-binding domains (PTB1 and PTB2), a calmodulin-binding domain (CBD), a Rab-GTPase domain, and multiple AKT phosphomotifs. This protein is thought to play an important role in glucose homeostasis by regulating the insulin-dependent trafficking of the glucose transporter 4 (GLUT4), important for removing glucose from the bloodstream into skeletal muscle and fat tissues. Reduced expression of this gene results in an increase in GLUT4 levels at the plasma membrane, suggesting that this protein is important in intracellular retention of GLUT4 under basal conditions. When exposed to insulin, this protein is phosphorylated, dissociates from GLUT4 vesicles, resulting in increased GLUT4 at the cell surface, and enhanced glucose transport. Phosphorylation of this protein by AKT is required for proper translocation of GLUT4 to the cell surface. Individuals homozygous for a mutation in this gene are at higher risk for type 2 diabetes and have higher levels of circulating glucose and insulin levels after glucose ingestion. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.