ATP2B1 (NM_001682) Human Untagged Clone

CAT#: SC309942

ATP2B1 (untagged)-Human ATPase, Ca++ transporting, plasma membrane 1 (ATP2B1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ATP2B1
• Synonyms: PMCA1; PMCA1kb
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001682 edited
  GCCACCATGGGCGACATGGCAAACAACTCAGTTGCTTACAGTGGTGTGAAAAACTCTTTG
  AAGGAAGCTAATCATGATGGAGACTTTGGAATTACGCTCGCAGAGCTGCGGGCTCTCATG
  GAGCTCAGGTCCACAGATGCATTACGAAAAATACAGGAAAGCTATGGAGATGTCTATGGA
  ATTTGCACCAAATTGAAAACATCTCCCAATGAAGGTTTAAGTGGAAACCCTGCAGATTTA
  GAAAGAAGAGAAGCAGTGTTTGGAAAGAATTTTATACCTCCTAAAAAGCCAAAAACCTTT
  CTTCAATTAGTATGGGAAGCATTACAAGATGTCACTTTAATTATATTAGAAATTGCAGCC
  ATAGTATCATTGGGCCTTTCTTTTTATCAGCCTCCAGAAGGGGATAATGCACTTTGTGGA
  GAAGTTTCTGTTGGGGAGGAAGAAGGTGAAGGTGAAACTGGTTGGATTGAAGGAGCTGCA
  ATCCTCTTGTCTGTAGTGTGTGTGGTGTTAGTAACAGCTTTCAATGACTGGAGTAAGGAA
  AAACAGTTTAGAGGTTTGCAGAGCCGAATTGAACAAGAACAGAAGTTCACTGTCATCAGG
  GGTGGTCAGGTCATTCAGATACCTGTAGCTGACATTACTGTTGGAGATATTGCTCAAGTG
  AAATATGGTGATCTTCTTCCAGCTGACGGCATACTTATTCAAGGCAACGATCTTAAAATT
  GATGAAAGCTCATTGACTGGTGAATCAGATCATGTTAAAAAGTCTTTAGATAAGGATCCC
  TTACTTCTATCAGGTACTCATGTAATGGAAGGCTCTGGAAGAATGGTAGTTACAGCTGTA
  GGTGTAAATTCTCAAACTGGAATTATCTTTACCTTACTTGGAGCTGGAGGTGAAGAGGAA
  GAGAAGAAAGATGAGAAGAAAAAGGAAAAGAAAAATAAGAAACAAGATGGAGCTATTGAG
  AATCGCAACAAAGCAAAAGCCCAGGATGGTGCAGCCATGGAAATGCAGCCATTGAAGAGT
  GAAGAAGGTGGAGATGGTGATGAAAAAGATAAAAAGAAAGCAAATTTGCCAAAAAAGGAA
  AAATCTGTTTTACAAGGGAAACTTACAAAACTGGCTGTTCAGATTGGCAAAGCAGGTCTG
  TTGATGTCTGCCATCACAGTTATCATTCTAGTATTATATTTTGTCATTGACACCTTCTGG
  GTTCAGAAAAGACCATGGCTTGCTGAGTGCACACCAATTTATATACAATACTTTGTGAAG
  TTCTTCATTATTGGAGTTACAGTTTTAGTGGTCGCAGTGCCAGAAGGTCTTCCACTTGCA
  GTCACGATCTCACTGGCTTATTCAGTCAAAAAAATGATGAAAGATAATAACTTAGTAAGG
  CATCTGGATGCTTGTGAAACCATGGGAAATGCTACAGCTATTTGTTCAGATAAAACAGGA
  ACTTTGACAATGAACAGAATGACAGTCGTTCAAGCTTACATAAATGAAAAACATTATAAA
  AAGGTTCCTGAACCAGAAGCTATTCCACCAAATATTTTGTCCTATCTTGTAACAGGAATT
  TCTGTGAATTGTGCTTATACATCAAAAATATTGCCACCAGAGAAAGAGGGTGGATTACCT
  CGTCACGTTGGTAATAAAACTGAATGTGCCTTGTTGGGACTTCTTTTGGATTTAAAACGG
  GATTATCAGGATGTTAGAAATGAAATACCAGAAGAAGCACTGTACAAAGTCTACACCTTC
  AATTCTGTTAGGAAGTCCATGAGTACTGTCCTGAAAAATTCAGATGGAAGTTATCGAATA
  TTCAGCAAGGGTGCATCTGAGATAATTCTGAAAAAGTGTTTCAAAATCTTGAGTGCTAAT
  GGTGAGGCAAAAGTATTCAGACCAAGGGACCGTGATGATATTGTAAAAACTGTGATTGAA
  CCGATGGCATCAGAAGGCTTGAGAACCATATGTCTTGCATTCAGAGATTTTCCAGCAGGA
  GAACCAGAACCAGAGTGGGATAATGAAAATGATATTGTCACCGGCCTTACATGCATTGCT
  GTTGTGGGGATTGAAGATCCTGTGAGACCTGAGGTGCCAGATGCAATTAAAAAGTGTCAG
  AGGGCTGGAATTACTGTGCGGATGGTCACTGGTGATAATATTAATACTGCTCGGGCCATT
  GCTACCAAATGTGGTATTTTACATCCTGGGGAAGATTTTCTGTGCCTAGAAGGTAAAGAT
  TTTAACAGAAGAATACGAAATGAAAAAGGAGAGATTGAGCAAGAGAGGATAGACAAGATT
  TGGCCAAAACTTCGAGTACTTGCAAGATCATCTCCTACTGATAAGCATACACTGGTTAAA
  GGTATAATTGACAGCACTGTCTCAGACCAACGCCAGGTTGTAGCTGTAACTGGTGATGGT
  ACAAATGATGGCCCAGCACTAAAGAAAGCAGATGTTGGATTTGCAATGGGTATTGCTGGA
  ACTGATGTAGCTAAAGAAGCATCCGATATTATTCTCACAGATGACAACTTTACAAGCATT
  GTTAAAGCAGTTATGTGGGGACGAAATGTCTATGACAGCATCTCAAAATTCCTTCAGTTC
  CAACTTACTGTTAATGTAGTAGCAGTGATTGTTGCTTTTACGGGCGCCTGCATTACTCAA
  GACTCACCGCTTAAGGCTGTGCAGATGCTGTGGGTAAACCTCATAATGGATACACTCGCT
  TCCCTGGCTCTGGCAACGGAACCACCCACTGAGTCTCTCTTGCTTCGGAAACCTTATGGT
  AGAAATAAGCCTCTCATCTCACGTACAATGATGAAGAATATTTTGGGTCATGCATTCTAT
  CAACTTGTAGTAGTCTTTACACTCTTATTTGCTGGAGAAAAGTTTTTTGACATTGATAGT
  GGAAGAAATGCTCCTTTGCATGCTCCTCCTTCAGAACATTATACTATTGTTTTTAATACC
  TTTGTGCTGATGCAACTTTTCAACGAAATAAATGCCCGGAAAATTCATGGTGAAAGAAAT
  GTATTCGAAGGAATCTTTAACAATGCCATCTTCTGCACAATTGTTTTAGGCACTTTTGTG
  GTACAGATAATAATTGTGCAGTTTGGTGGAAAACCTTTCAGTTGTTCAGAACTTTCAATA
  GAACAGTGGCTATGGTCAATATTCCTAGGAATGGGAACATTACTCTGGGGCCAGCTTATT
  TCAACAATTCCAACTAGCCGTTTAAAATTCCTCAAAGAAGCTGGTCATGGAACACAAAAG
  GAAGAAATACCTGAGGAGGAATTAGCAGAGGATGTTGAAGAGATTGATCACGCTGAAAGG
  GAGTTGCGGCGTGGCCAAATCTTGTGGTTTAGAGGTCTGAACAGAATCCAAACACAGATT
  CGAGTGGTGAATGCATTTCGTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001682
• ORF Size: 3663 bp
• Insert Size: 3300
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_001682.2, NP_001673.2
• RefSeq Size: 6796
• RefSeq ORF: 3663
• Locus ID: 490
• Domains: E1-E2_ATPase, Cation_ATPase_N, Hydrolase, Cation_ATPase_C
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Calcium signaling pathway
• Gene Summary: The protein encoded by this gene belongs to the family of P-type primary ion transport ATPases characterized by the formation of an aspartyl phosphate intermediate during the reaction cycle. These enzymes remove bivalent calcium ions from eukaryotic cells against very large concentration gradients and play a critical role in intracellular calcium homeostasis. The mammalian plasma membrane calcium ATPase isoforms are encoded by at least four separate genes and the diversity of these enzymes is further increased by alternative splicing of transcripts. The expression of different isoforms and splice variants is regulated in a developmental, tissue- and cell type-specific manner, suggesting that these pumps are functionally adapted to the physiological needs of particular cells and tissues. This gene encodes the plasma membrane calcium ATPase isoform 1. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks a segment in the coding region, which leads to a frameshift, compared to variant 1. The resulting isoform (1b) contains a longer and distinct C-terminus compared to isoform 1a. Variants 2, 3, 4, and 5 all encode the same isoform (1b). Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments.