MAP4K4 (NM_004834) Human Untagged Clone

CAT#: SC309964

MAP4K4 (untagged)-Human mitogen-activated protein kinase kinase kinase kinase 4 (MAP4K4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MAP4K4
• Synonyms: FLH21957; HEL-S-31; HGK; MEKKK4; NIK
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_004834 edited
  CTCACTCGCTCAACTCTCGGCGCCGCCGCGGCCCCACGCTCCGGGCCCGTCCTCGAGGCG
  CGCGGCGCGGGGCGCGGGCGCCGGGGCCTGAGGCGGCGGGCGACGCCCGGGGGCCTGACG
  GCCGGCCCCGCGCCATGGTGTGAGCGCCGCCGCCCGTGCACGCTCCGTCCGCCCTCCGCG
  CGGCCCGGCCGGCAGAGAGCCCCGAGCGGCCCGAGAGCGCAGCCGAGCCCGCCGCCGCCG
  CCCGCGGCCCCGCGAGGAGAGTACCGGGCCGGCCCGGCTGCCGCGCGAGGAGCGCGGTCG
  GCGGCCTGGTCTGCGGCTGAGATACACAGAGCGACAGAGACATTTATTGTTATTTGTTTT
  TTGGTGGCAAAAAGGGAAAATGGCGAACGACTCCCCTGCAAAAAGTCTGGTGGACATCGA
  CCTCTCCTCCCTGCGGGATCCTGCTGGGATTTTTGAGCTGGTGGAAGTGGTTGGAAATGG
  CACCTATGGACAAGTCTATAAGGGTCGACATGTTAAAACGGGTCAGTTGGCAGCCATCAA
  AGTTATGGATGTCACTGAGGATGAAGAGGAAGAAATCAAACTGGAGATAAATATGCTAAA
  GAAATACTCTCATCACAGAAACATTGCAACATATTATGGTGCTTTCATCAAAAAGAGCCC
  TCCAGGACATGATGACCAACTCTGGCTTGTTATGGAGTTCTGTGGGGCTGGGTCCATTAC
  AGACCTTGTGAAGAACACCAAAGGGAACACACTCAAAGAAGACTGGATCGCTTACATCTC
  CAGAGAAATCCTGAGGGGACTGGCACATCTTCACATTCATCATGTGATTCACCGGGATAT
  CAAGGGCCAGAATGTGTTGCTGACTGAGAATGCAGAGGTGAAACTTGTTGACTTTGGTGT
  GAGTGCTCAGCTGGACAGGACTGTGGGGCGGAGAAATACGTTCATAGGCACTCCCTACTG
  GATGGCTCCTGAGGTCATCGCCTGTGATGAGAACCCAGATGCCACCTATGATTACAGAAG
  TGATCTTTGGTCTTGTGGCATTACAGCCATTGAGATGGCAGAAGGTGCTCCCCCTCTCTG
  TGACATGCATCCAATGAGAGCACTGTTTCTCATTCCCAGAAACCCTCCTCCCCGGCTGAA
  GTCAAAAAAATGGTCGAAGAAGTTTTTTAGTTTTATAGAAGGGTGCCTGGTGAAGAATTA
  CATGCAGCGGCCCTCTACAGAGCAGCTTTTGAAACATCCTTTTATAAGGGATCAGCCAAA
  TGAAAGGCAAGTTAGAATCCAGCTTAAGGATCATATAGATCGTACCAGGAAGAAGAGAGG
  CGAGAAAGATGAAACTGAGTATGAGTACAGTGGGAGTGAGGAAGAAGAGGAGGAAGTGCC
  TGAACAGGAAGGAGAGCCAAGTTCCATTGTGAACGTGCCTGGTGAGTCTACTCTTCGCCG
  AGATTTCCTGAGACTGCAGCAGGAGAACAAGGAACGTTCCGAGGCTCTTCGGAGACAACA
  GTTACTACAGGAGCAACAGCTCCGGGAGCAGGAAGAATATAAAAGGCAACTGCTGGCAGA
  GAGACAGAAGCGGATTGAGCAGCAGAAAGAACAGAGGCGACGGCTAGAAGAGCAACAAAG
  GAGAGAGCGGGAAGCTAGAAGGCAGCAGGAACGTGAACAGCGAAGGAGAGAACAAGAAGA
  AAAGAGGCGTCTAGAGGAGTTGGAGAGAAGGCGCAAAGAAGAAGAGGAGAGGAGACGGGC
  AGAAGAAGAAAAGAGGAGAGTTGAAAGAGAACAGGAGTATATCAGGCGACAGCTAGAAGA
  GGAGCAGCGGCACTTGGAAGTCCTTCAGCAGCAGCTGCTCCAGGAGCAGGCCATGTTACT
  GCATGACCATAGGAGGCCGCACCCGCAGCACTCGCAGCAGCCGCCACCACCGCAGCAGGA
  AAGGAGCAAGCCAAGCTTCCATGCTCCCGAGCCCAAAGCCCACTACGAGCCTGCTGACCG
  AGCGCGAGAGGTTCCTGTGAGAACAACATCTCGCTCCCCTGTTCTGTCCCGTCGAGATTC
  CCCACTGCAGGGCAGTGGGCAGCAGAATAGCCAGGCAGGACAGAGAAACTCCACCAGTAT
  TGAGCCCAGGCTTCTGTGGGAGAGAGTGGAGAAGCTGGTGCCCAGACCTGGCAGTGGCAG
  CTCCTCAGGGTCCAGCAACTCAGGATCCCAGCCCGGGTCTCACCCTGGGTCTCAGAGTGG
  CTCCGGGGAACGCTTCAGAGTGAGATCATCATCCAAGTCTGAAGGCTCTCCATCTCAGCG
  CCTGGAAAATGCAGTGAAAAAACCTGAAGATAAAAAGGAAGTTTTCAGACCCCTCAAGCC
  TGCTGGCGAAGTGGATCTGACCGCACTGGCCAAAGAGCTTCGAGCAGTGGAAGATGTACG
  GCCACCTCACAAAGTAACGGACTACTCCTCATCCAGTGAGGAGTCGGGGACGACGGATGA
  GGAGGACGACGATGTGGAGCAGGAAGGGGCTGACGAGTCCACCTCAGGACCAGAGGACAC
  CAGAGCAGCGTCATCTCTGAATTTGAGCAATGGTGAAACGGAATCTGTGAAAACCATGAT
  TGTCCATGATGATGTAGAAAGTGAGCCGGCCATGACCCCATCCAAGGAGGGCACTCTAAT
  CGTCCGCCAGACTCAGTCCGCTAGTAGCACACTCCAGAAACACAAATCTTCCTCCTCCTT
  TACACCTTTTATAGACCCCAGATTACTACAGATTTCTCCATCTAGCGGAACAACAGTGAC
  ATCTGTGGTGGGATTTTCCTGTGATGGGATGAGACCAGAAGCCATAAGGCAAGATCCTAC
  CCGGAAAGGCTCAGTGGTCAATGTGAATCCTACCAACACTAGGCCACAGAGTGACACCCC
  GGAGATTCGTAAATACAAGAAGAGGTTTAACTCTGAGATTCTGTGTGCTGCCTTATGGGG
  AGTGAATTTGCTAGTGGGTACAGAGAGTGGCCTGATGCTGCTGGACAGAAGTGGCCAAGG
  GAAGGTCTATCCTCTTATCAACCGAAGACGATTTCAACAAATGGACGTACTTGAGGGCTT
  GAATGTCTTGGTGACAATATCTGGCAAAAAGGATAAGTTACGTGTCTACTATTTGTCCTG
  GTTAAGAAATAAAATACTTCACAATGATCCAGAAGTTGAGAAGAAGCAGGGATGGACAAC
  CGTAGGGGATTTGGAAGGATGTGTACATTATAAAGTTGTAAAATATGAAAGAATCAAATT
  TCTGGTGATTGCTTTGAAGAGTTCTGTGGAAGTCTATGCGTGGGCACCAAAGCCATATCA
  CAAATTTATGGCCTTTAAGTCATTTGGAGAATTGGTACATAAGCCATTACTGGTGGATCT
  CACTGTTGAGGAAGGCCAGAGGTTGAAAGTGATCTATGGATCCTGTGCTGGATTCCATGC
  TGTTGATGTGGATTCAGGATCAGTCTATGACATTTATCTACCAACACATATCCAGTGTAG
  CATCAAACCCCATGCAATCATCATCCTCCCCAATACAGATGGAATGGAGCTTCTGGTGTG
  CTATGAAGATGAGGGGGTTTATGTAAACACATATGGAAGGATCACCAAGGATGTAGTTCT
  ACAGTGGGGAGAGATGCCTACATCAGTAGCATATATTCGATCCAATCAGACAATGGGCTG
  GGGAGAGAAGGCCATAGAGATCCGATCTGTGGAAACTGGTCACTTGGATGGTGTGTTCAT
  GCACAAAAGGGCTCAAAGACTAAAATTCTTGTGTGAACGCAATGACAAGGTGTTCTTTGC
  CTCTGTTCGGTCTGGTGGCAGCAGTCAGGTTTATTTCATGACCTTAGGCAGGACTTCTCT
  TCTGAGCTGGTAGAAGCAGTGTGATCCAGGGATTACTGGCCTCCAGAGTCTTCAAGATCC
  TGAGAACTTGGAATTCCTTGTAACTGGAGCTCGGAGCTGCACCGAGGGCAACCAGGACAG
  CTGTGTGTGCAGACCTCATGTGTTGGGTTCTCTCCCCTCCTTCCTGTTCCTCTTATATAC
  CAGTTTATCCCCATTCTTTTTTTTTTTCTTACTCCAAAATAAATCAAGGCTGCAATGCAG
  CTGGTGCTGTTCAGATTCTACCATCAGGTGCTATAAGTGTTTGGGATTGAGCATCATACT
  GGAAAGCAAACACCTTTCCTCCAGCTCCAGAATTCCTTGTCTCTGAATGACTCTGTCTTG
  TGGGTGTCTGACAGTGGCGACGATGAACATGCCGTTGGTTTTATTGGCAGTGGGCACAAG
  GAGGTGAGAAGTGGTGGTAAAAGGAGCAGAGTGCTGAAGCAGAGAGCAGATTTAATATAG
  TAACATTAACAGTGTATTTAATTGACATTTCTTTTTTGTAATGTGACGATATGTGGACAA
  AGAAGAAGATGCAGGTTTAAGAAGTTAATATTTATAAAATGTGAAAGACACAGTTACTAG
  GATAACTTTTTTGTGGGTGGGGCTTGGGAGATGGGGTGGGGTGGGTTAAGGGGTCCCATT
  TTGTTTCTTTGGATTTGGGGTGGGGGTCCTGGCCAAGAACTCAGTCATTTTTCTGTGTAC
  CAGGTTGCCTAAATCATGTGCAGATGGTTCTAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_004834
• ORF Size: 3501 bp
• Insert Size: 4800
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone has been fully sequenced and its ORF is found to match with NM_004834.3 except 9 amino acids are missing. This clone has a toxic effect. This clone may be unstable or toxic at high copy number in common E. coli strain. We recommend using a lower copy number E. coli strain, such as CopyCutter strain (http://www.epibio.com/item.asp?ID=435) for transformation and plasmid preparation. Please be aware that the DNA yield could be low. Additional aliquots of this clone can be ordered from OriGene.

Reference Data

• RefSeq: NM_004834.3, NP_004825.2
• RefSeq Size: 7033
• RefSeq ORF: 3501
• Locus ID: 9448
• Domains: pkinase, CNH, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: MAPK signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the serine/threonine protein kinase family. This kinase has been shown to specifically activate MAPK8/JNK. The activation of MAPK8 by this kinase is found to be inhibited by the dominant-negative mutants of MAP3K7/TAK1, MAP2K4/MKK4, and MAP2K7/MKK7, which suggests that this kinase may function through the MAP3K7-MAP2K4-MAP2K7 kinase cascade, and mediate the TNF-alpha signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) lacks two alternate in-frame exons but includes a different in-frame exon in the central coding region, and uses an alternate in-frame splice site in both the central and 3' coding regions, compared to variant 2. The encoded isoform (1) is shorter than isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.