DIAPH2 (NM_006729) Human Untagged Clone

CAT#: SC309982

DIAPH2 (untagged)-Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 156

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DIAPH2
• Synonyms: DIA; DIA2; DRF2; POF; POF2; POF2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_006729, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGCAGCCCGGGGCGGCGGCGTCGGGAGCGGGAGGCGGCAGCGAGGAACCCGGTGGGGGCCGGAGCA
  ACAAGCGGAGCGCGGGGAACCGGGCCGCCAATGAAGAGGAAACGAAAAACAAACCCAAATTGAACATTCA
  AATAAAAACTTTGGCAGATGATGTGCGTGACCGAATTACAAGTTTTAGAAAATCTACTGTCAAAAAAGAA
  AAACCTCTTATTCAACATCCTATTGATTCTCAAGTCGCGATGAGTGAGTTTCCTGCAGCTCAGCCATTAT
  ATGATGAACGATCTTTGAATTTGTCAGAAAAGGAAGTATTGGATCTCTTTGAAAAAATGATGGAGGACAT
  GAACCTTAACGAAGAGAAAAAAGCTCCTTTACGAAACAAAGACTTTACCACCAAACGTGAGATGGTTGTC
  CAGTATATTTCTGCCACTGCCAAATCTGGTGGGCTGAAAAACAGCAAACATGAATGCACCCTGTCTTCAC
  AAGAATATGTTCATGAATTACGATCGGGTATATCAGATGAGAAACTTCTTAATTGCCTAGAATCCCTCAG
  GGTTTCTTTAACCAGCAATCCGGTCAGCTGGGTTAACAACTTTGGCCATGAAGGTCTTGGACTCTTATTG
  GATGAGCTGGAAAAGCTTCTGGACAAAAAACAGCAAGAAAATATTGACAAGAAGAATCAGTATAAACTTA
  TTCAATGCCTCAAAGCATTTATGAATAATAAGTTTGGATTACAAAGGATTCTAGGAGATGAAAGAAGTCT
  TTTACTATTGGCAAGAGCAATTGACCCCAAACAACCCAACATGATGACTGAAATAGTAAAAATACTTTCT
  GCTATTTGCATTGTTGGAGAAGAGAACATTCTAGATAAACTTTTAGGGGCTATAACAACAGCAGCAGAAA
  GAAATAACAGGGAACGATTTTCACCAATTGTGGAAGGTTTAGAAAATCAGGAAGCCTTGCAATTACAGGT
  GGCCTGCATGCAGTTTATAAATGCCCTTGTCACTTCTCCTTATGAGCTTGATTTTCGAATACATTTAAGG
  AATGAATTCCTCCGTTCAGGACTAAAAACAATGTTACCAGATCTAAAAGAAAAAGAGAATGATGAGCTTG
  ATATTCAGTTGAAAGTATTTGATGAAAACAAAGAAGATGACCTAACTGAATTATCACACCGTCTCAATGA
  CATTCGAGCAGAAATGGATGATATGAATGAAGTCTACCATCTTCTATATAATATGCTGAAGGACACTGCT
  GCTGAAAATTACTTCTTATCTATTCTACAACATTTTTTGCTTATCAGAAATGATTATTATATCAGGCCAC
  AATATTATAAAATAATTGAGGAATGTGTTTCACAGATAGTGCTACACTGCAGTGGTATGGATCCAGACTT
  CAAATACAGGCAAAGATTAGACATCGATTTAACTCATCTGATAGATTCTTGTGTGAACAAGGCGAAAGTT
  GAAGAAAGTGAACAAAAAGCTGCAGAGTTTTCAAAGAAGTTCGATGAAGAATTCACAGCTCGACAGGAAG
  CTCAAGCAGAGCTTCAAAAAAGAGATGAGAAAATCAAAGAACTTGAAGCAGAAATCCAGCAACTTCGAAC
  CCAGGCACAAGTACTCTCAAGTTCATCAGGAATTCCAGGTCCTCCTGCAGCACCTCCATTGCCAGGTGTA
  GGGCCGCCTCCACCACCACCCGCGCCACCTCTACCCGGAGGAGCTCCTCTTCCTCCTCCACCACCTCCTT
  TACCTGGAATGATGGGGATACCACCACCACCCCCACCACCACTTTTATTTGGGGGACCTCCTCCACCACC
  ACCCCTTGGAGGAGTTCCTCCTCCCCCAGGAATATCACTTAATCTACCTTATGGAATGAAGCAGAAAAAA
  ATGTATAAACCTGAAGTGTCCATGAAGAGAATCAATTGGTCAAAGATTGAACCCACAGAATTATCTGAGA
  ACTGTTTCTGGTTAAGAGTCAAAGAAGACAAGTTTGAGAATCCAGATCTCTTTGCCAAATTGGCATTGAA
  TTTTGCTACTCAGATAAAAGTTCAAAAGAACGCAGAAGCATTAGAAGAAAAGAAGACTGGGCCTACAAAG
  AAGAAAGTGAAAGAACTGAGAATTTTGGATCCCAAAACAGCTCAGAATCTGTCCATCTTTCTGGGATCAT
  ATCGCATGCCATATGAAGACATAAGAAACGTTATTCTGGAGGTTAATGAAGACATGCTGAGTGAGGCTTT
  AATTCAGAACCTTGTGAAACATCTTCCTGAGCAGAAGATACTCAACGAATTAGCAGAGCTTAAGAATGAA
  TATGATGACCTCTGTGAGCCTGAACAATTTGGAGTTGTGATGAGCTCTGTGAAAATGTTACAGCCTCGTC
  TCAGTAGTATCCTGTTCAAGCTCACATTTGAAGAACACATAAACAACATCAAACCAAGCATCATAGCAGT
  AACTCTTGCCTGTGAAGAACTGAAGAAAAGTGAAAGCTTTAACAGACTTTTAGAGTTAGTTCTTCTTGTT
  GGAAACTACATGAACTCAGGCTCAAGAAATGCCCAGTCTTTGGGATTTAAGATCAACTTCCTTTGTAAGA
  TCAGAGATACTAAATCAGCGGATCAAAAAACAACCCTTTTGCATTTTATTGCCGACATTTGTGAGGAAAA
  ATATCGAGATATCCTAAAATTTCCTGAAGAACTGGAACACGTAGAAAGTGCAAGCAAAGTTTCAGCTCAA
  ATTCTCAAGAGCAACCTTGCATCAATGGAACAACAAATTGTTCATCTGGAACGTGACATCAAGAAATTCC
  CCCAAGCAGAAAATCAACACGATAAGTTTGTGGAAAAGATGACCAGCTTTACAAAGACTGCCCGAGAACA
  GTATGAAAAACTCTCCACCATGCACAACAACATGATGAAGCTCTATGAGAATCTTGGAGAATACTTCATT
  TTTGACTCAAAGACAGTGAGCATAGAAGAGTTCTTTGGTGATCTCAACAACTTCCGAACTTTGTTTTTGG
  AAGCAGTGAGAGAAAACAATAAGAGAAGAGAAATGGAAGAGAAGACCAGGAGGGCAAAACTTGCAAAAGA
  GAAAGCTGAACAAGAAAAGTTAGAACGCCAGAAGAAAAAGAAACAACTCATTGATATAAACAAAGAGGGT
  GATGAGACTGGTGTGATGGATAATCTTCTAGAAGCCCTACAATCAGGTGCAGCATTCAGAGACCGTCGAA
  AGCGGATTCCAAGGAATCCAGATAACAGACGAGTACCTTTGGAAAGGTCACGCTCTCGCCACAATGGAGC
  TATCTCATCTAAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_006729
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006729.4, NP_006720.1
• RefSeq Size: 4983 bp
• RefSeq ORF: 3306 bp
• Locus ID: 1730
• Cytogenetics: Xq21.33
• Domains: FH2
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton
• Gene Summary: 'The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (156) represents the longer transcript, and encodes the longer isoform (isoform 156).