DIAPH2 (NM_006729) Human Tagged ORF Clone

CAT#: RC215274

DIAPH2 (Myc-DDK-tagged)-Human diaphanous homolog 2 (Drosophila) (DIAPH2), transcript variant 156

Product Images

Western blot validation of overexpression lysate (Cat# LY416466) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC215274 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DIAPH2
• Synonyms: DIA; DIA2; DRF2; POF; POF2; POF2A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC215274 representing NM_006729
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCAGCCCGGGGCGGCGGCGTCGGGAGCGGGAGGCGGCAGCGAGGAACCCGGTGGGGGCCGGAGCA
  ACAAGCGGAGCGCGGGGAACCGGGCCGCCAATGAAGAGGAAACGAAAAACAAACCCAAATTGAACATTCA
  AATAAAAACTTTGGCAGATGATGTGCGTGACCGAATTACAAGTTTTAGAAAATCTACTGTCAAAAAAGAA
  AAACCTCTTATTCAACATCCTATTGATTCTCAAGTCGCGATGAGTGAGTTTCCTGCAGCTCAGCCATTAT
  ATGATGAACGATCTTTGAATTTGTCAGAAAAGGAAGTATTGGATCTCTTTGAAAAAATGATGGAGGACAT
  GAACCTTAACGAAGAGAAAAAAGCTCCTTTACGAAACAAAGACTTTACCACCAAACGTGAGATGGTTGTC
  CAGTATATTTCTGCCACTGCCAAATCTGGTGGGCTGAAAAACAGCAAACATGAATGCACCCTGTCTTCAC
  AAGAATATGTTCATGAATTACGATCGGGTATATCAGATGAGAAACTTCTTAATTGCCTAGAATCCCTCAG
  GGTTTCTTTAACCAGCAATCCGGTCAGCTGGGTTAACAACTTTGGCCATGAAGGTCTTGGACTCTTATTG
  GATGAGCTGGAAAAGCTTCTGGACAAAAAACAGCAAGAAAATATTGACAAGAAGAATCAGTATAAACTTA
  TTCAATGCCTCAAAGCATTTATGAATAATAAGTTTGGATTACAAAGGATTCTAGGAGATGAAAGAAGTCT
  TTTACTATTGGCAAGAGCAATTGACCCCAAACAACCCAACATGATGACTGAAATAGTAAAAATACTTTCT
  GCTATTTGCATTGTTGGAGAAGAGAACATTCTAGATAAACTTTTAGGGGCTATAACAACAGCAGCAGAAA
  GAAATAACAGGGAACGATTTTCACCAATTGTGGAAGGTTTAGAAAATCAGGAAGCCTTGCAATTACAGGT
  GGCCTGCATGCAGTTTATAAATGCCCTTGTCACTTCTCCTTATGAGCTTGATTTTCGAATACATTTAAGG
  AATGAATTCCTCCGTTCAGGACTAAAAACAATGTTACCAGATCTAAAAGAAAAAGAGAATGATGAGCTTG
  ATATTCAGTTGAAAGTATTTGATGAAAACAAAGAAGATGACCTAACTGAATTATCACACCGTCTCAATGA
  CATTCGAGCAGAAATGGATGATATGAATGAAGTCTACCATCTTCTATATAATATGCTGAAGGACACTGCT
  GCTGAAAATTACTTCTTATCTATTCTACAACATTTTTTGCTTATCAGAAATGATTATTATATCAGGCCAC
  AATATTATAAAATAATTGAGGAATGTGTTTCACAGATAGTGCTACACTGCAGTGGTATGGATCCAGACTT
  CAAATACAGGCAAAGATTAGACATCGATTTAACTCATCTGATAGATTCTTGTGTGAACAAGGCGAAAGTT
  GAAGAAAGTGAACAAAAAGCTGCAGAGTTTTCAAAGAAGTTCGATGAAGAATTCACAGCTCGACAGGAAG
  CTCAAGCAGAGCTTCAAAAAAGAGATGAGAAAATCAAAGAACTTGAAGCAGAAATCCAGCAACTTCGAAC
  CCAGGCACAAGTACTCTCAAGTTCATCAGGAATTCCAGGTCCTCCTGCAGCACCTCCATTGCCAGGTGTA
  GGGCCGCCTCCACCACCACCCGCGCCACCTCTACCCGGAGGAGCTCCTCTTCCTCCTCCACCACCTCCTT
  TACCTGGAATGATGGGGATACCACCACCACCCCCACCACCACTTTTATTTGGGGGACCTCCTCCACCACC
  ACCCCTTGGAGGAGTTCCTCCTCCCCCAGGAATATCACTTAATCTACCTTATGGAATGAAGCAGAAAAAA
  ATGTATAAACCTGAAGTGTCCATGAAGAGAATCAATTGGTCAAAGATTGAACCCACAGAATTATCTGAGA
  ACTGTTTCTGGTTAAGAGTCAAAGAAGACAAGTTTGAGAATCCAGATCTCTTTGCCAAATTGGCATTGAA
  TTTTGCTACTCAGATAAAAGTTCAAAAGAACGCAGAAGCATTAGAAGAAAAGAAGACTGGGCCTACAAAG
  AAGAAAGTGAAAGAACTGAGAATTTTGGATCCCAAAACAGCTCAGAATCTGTCCATCTTTCTGGGATCAT
  ATCGCATGCCATATGAAGACATAAGAAACGTTATTCTGGAGGTTAATGAAGACATGCTGAGTGAGGCTTT
  AATTCAGAACCTTGTGAAACATCTTCCTGAGCAGAAGATACTCAACGAATTAGCAGAGCTTAAGAATGAA
  TATGATGACCTCTGTGAGCCTGAACAATTTGGAGTTGTGATGAGCTCTGTGAAAATGTTACAGCCTCGTC
  TCAGTAGTATCCTGTTCAAGCTCACATTTGAAGAACACATAAACAACATCAAACCAAGCATCATAGCAGT
  AACTCTTGCCTGTGAAGAACTGAAGAAAAGTGAAAGCTTTAACAGACTTTTAGAGTTAGTTCTTCTTGTT
  GGAAACTACATGAACTCAGGCTCAAGAAATGCCCAGTCTTTGGGATTTAAGATCAACTTCCTTTGTAAGA
  TCAGAGATACTAAATCAGCGGATCAAAAAACAACCCTTTTGCATTTTATTGCCGACATTTGTGAGGAAAA
  ATATCGAGATATCCTAAAATTTCCTGAAGAACTGGAACACGTAGAAAGTGCAAGCAAAGTTTCAGCTCAA
  ATTCTCAAGAGCAACCTTGCATCAATGGAACAACAAATTGTTCATCTGGAACGTGACATCAAGAAATTCC
  CCCAAGCAGAAAATCAACACGATAAGTTTGTGGAAAAGATGACCAGCTTTACAAAGACTGCCCGAGAACA
  GTATGAAAAACTCTCCACCATGCACAACAACATGATGAAGCTCTATGAGAATCTTGGAGAATACTTCATT
  TTTGACTCAAAGACAGTGAGCATAGAAGAGTTCTTTGGTGATCTCAACAACTTCCGAACTTTGTTTTTGG
  AAGCAGTGAGAGAAAACAATAAGAGAAGAGAAATGGAAGAGAAGACCAGGAGGGCAAAACTTGCAAAAGA
  GAAAGCTGAACAAGAAAAGTTAGAACGCCAGAAGAAAAAGAAACAACTCATTGATATAAACAAAGAGGGT
  GATGAGACTGGTGTGATGGATAATCTTCTAGAAGCCCTACAATCAGGTGCAGCATTCAGAGACCGTCGAA
  AGCGGATTCCAAGGAATCCAGATAACAGACGAGTACCTTTGGAAAGGTCACGCTCTCGCCACAATGGAGC
  TATCTCATCTAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC215274 representing NM_006729
  Red=Cloning site Green=Tags(s)
  
  MEQPGAAASGAGGGSEEPGGGRSNKRSAGNRAANEEETKNKPKLNIQIKTLADDVRDRITSFRKSTVKKE
  KPLIQHPIDSQVAMSEFPAAQPLYDERSLNLSEKEVLDLFEKMMEDMNLNEEKKAPLRNKDFTTKREMVV
  QYISATAKSGGLKNSKHECTLSSQEYVHELRSGISDEKLLNCLESLRVSLTSNPVSWVNNFGHEGLGLLL
  DELEKLLDKKQQENIDKKNQYKLIQCLKAFMNNKFGLQRILGDERSLLLLARAIDPKQPNMMTEIVKILS
  AICIVGEENILDKLLGAITTAAERNNRERFSPIVEGLENQEALQLQVACMQFINALVTSPYELDFRIHLR
  NEFLRSGLKTMLPDLKEKENDELDIQLKVFDENKEDDLTELSHRLNDIRAEMDDMNEVYHLLYNMLKDTA
  AENYFLSILQHFLLIRNDYYIRPQYYKIIEECVSQIVLHCSGMDPDFKYRQRLDIDLTHLIDSCVNKAKV
  EESEQKAAEFSKKFDEEFTARQEAQAELQKRDEKIKELEAEIQQLRTQAQVLSSSSGIPGPPAAPPLPGV
  GPPPPPPAPPLPGGAPLPPPPPPLPGMMGIPPPPPPPLLFGGPPPPPPLGGVPPPPGISLNLPYGMKQKK
  MYKPEVSMKRINWSKIEPTELSENCFWLRVKEDKFENPDLFAKLALNFATQIKVQKNAEALEEKKTGPTK
  KKVKELRILDPKTAQNLSIFLGSYRMPYEDIRNVILEVNEDMLSEALIQNLVKHLPEQKILNELAELKNE
  YDDLCEPEQFGVVMSSVKMLQPRLSSILFKLTFEEHINNIKPSIIAVTLACEELKKSESFNRLLELVLLV
  GNYMNSGSRNAQSLGFKINFLCKIRDTKSADQKTTLLHFIADICEEKYRDILKFPEELEHVESASKVSAQ
  ILKSNLASMEQQIVHLERDIKKFPQAENQHDKFVEKMTSFTKTAREQYEKLSTMHNNMMKLYENLGEYFI
  FDSKTVSIEEFFGDLNNFRTLFLEAVRENNKRREMEEKTRRAKLAKEKAEQEKLERQKKKKQLIDINKEG
  DETGVMDNLLEALQSGAAFRDRRKRIPRNPDNRRVPLERSRSRHNGAISSK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006729
• ORF Size: 3303 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006729.1, NM_006729.2, NM_006729.3, NM_006729.4, NP_006720.1
• RefSeq Size: 9344 bp
• RefSeq ORF: 3306 bp
• Locus ID: 1730
• Cytogenetics: Xq21.33
• Domains: FH2
• Protein Families: Druggable Genome
• Protein Pathways: Regulation of actin cytoskeleton
• MW: 125.4 kDa
• Gene Summary: 'The product of this gene belongs to the diaphanous subfamily of the formin homology family of proteins. This gene may play a role in the development and normal function of the ovaries. Defects in this gene have been linked to premature ovarian failure 2. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]'