GM130 (GOLGA2) (NM_004486) Human Untagged Clone

CAT#: SC310019

GOLGA2 (untagged)-Human golgin A2 (GOLGA2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GOLGA2
• Synonyms: GM130
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene SC310019 ORF sequence for NM_004486, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGTGGCCCCAACCCCGCCTCCCTCCCCGCCCCGCGATGTCGGAAGAAACCCGACAGAGCAAATTGGCCG
  CAGCGAAGAAAAAGTTGAGAGAATATCAGCAGAGGAATAGCCCTGGTGTTCCTACAGGAGCGAAAAAGAA
  GAAGAAAATAAAAAATGGCAGTAACCCTGAGACAACCACTTCTGGTGGTTGCCACTCACCTGAGGATACA
  CCCAAGGACAATGCTGCTACTCTACAACCATCTGATGACACCGTGTTACCTGGCGGTGTCCCTTCCCCTG
  GTGCCAGTCTCACTAGCATGGCGGCATCTCAGAATCATGATGCTGACAATGTCCCTAATCTCATGGATGA
  AACCAAGACTTTCTCATCAACCGAGAGCCTGCGACAACTCTCCCAACAGCTCAATGGTCTTGTTTGTGAG
  TCTGCGACATGTGTCAATGGGGAGGGCCCTGCATCGTCTGCTAACCTGAAGGATCTGGAGAGCCGGTACC
  AACAGCTAGCGGTAGCCCTGGACTCCAGCTATGTAACAAACAAACAACTCAATATCACGATAGAGAAATT
  GAAACAACAGAACCAAGAAATTACGGATCAGTTGGAAGAAGAAAAGAAAGAATGCCACCAAAAGCAGGGA
  GCCCTAAGGGAGCAGTTACAGGTTCACATTCAGACCATAGGGATCCTCGTATCAGAGAAAGCTGAGTTAC
  AGACAGCCCTGGCTCACACTCAGCATGCTGCCAGGCAGAAAGAAGGAGAGTCTGAAGATCTGGCCAGCCG
  CCTGCAGTATTCCCGGCGGCGTGTGGGAGAGTTGGAGCGGGCTCTCTCTGCTGTCTCCACGCAGCAGAAG
  AAGGCAGACAGGTACAACAAGGAGTTAACCAAAGAGAGAGACGCCCTCAGGCTGGAGTTATACAAGAACA
  CCCAAAGCAATGAGGACCTGAAGCAAGAGAAATCAGAATTGGAAGAGAAGCTTCGGGTCCTAGTGACTGA
  GAAGGCTGGCATGCAGCTTAACTTGGAAGAATTGCAAAAGAAGTTAGAGATGACGGAACTCCTGCTTCAA
  CAGTTTTCAAGCCGGTGTGAAGCCCCTGATGCTAACCAGCAGTTACAGCAGGCCATGGAGGAGCGGGCAC
  AGCTGGAAGCACACCTGGGGCAGGTAATGGAGTCGGTTAGACAACTACAAATGGAGAGAGATAAATATGC
  GGAGAATCTCAAAGGAGAGAGCGCCATGTGGCGGCAGAGGATGCAGCAGATGTCAGAGCAGGTGCACACA
  TTGAGAGAGGAGAAGGAATGTAGCATGAGTCGGGTACAGGAGCTGGAGACGAGCTTGGCTGAACTGAGGA
  ACCAGATGGCTGAACCCCCGCCCCCAGAGCCCCCAGCAGGGCCCTCCGAGGTGGAGCAGCAGCTACAAGC
  GGAGGCTGAGCACCTGCGGAAGGAGCTGGAGGGTCTGGCAGGACAGCTTCAAGCCCAGGTGCAAGACAAT
  GAGGGCTTGAGTCGCCTGAACCGGGAGCAGGAGGAGAGGCTGCTGGAGCTGGAGCGGGCGGCCGAGCTCT
  GGGGGGAGCAGGCGGAGGCGCGCAGGCAAATCCTGGAGACCATGCAGAACGACCGCACTACCATCAGCCG
  CGCACTCTCCCAGAACCGGGAGCTCAAGGAGCAGCTGGCTGAGCTGCAGAGCGGATTTGTAAAGCTGACT
  AATGAGAACATGGAGATCACCAGCGCACTGCAGTCGGAGCAGCACGTCAAGAGGGAGCTGGGAAAGAAGC
  TGGGCGAGCTGCAGGAGAAGCTGAGCGAGCTGAAGGAAACGGTGGAGCTGAAGAGCCAAGAGGCTCAAAG
  TCTGCAGCAGCAGCGAGACCAGTACCTGGGACACCTGCAGCAGTATGTGGCCGCCTATCAGCAGCTGACC
  TCTGAGAAGGAGGTGCTGCATAATCAGCTACTGCTGCAGACCCAGCTCGTGGACCAGCTGCAGCAGCAGG
  AAGCTCAGGGCAAAGCGGTGGCCGAGATGGCCCGCCAAGAGTTGCAGGAAACCCAGGAGCGCCTGGAAGC
  TGCCACCCAGCAGAATCAGCAGCTACGGGCCCAGTTGAGCCTCATGGCTCACCCTGGGGAAGGAGATGGA
  CTGGACCGGGAGGAGGAGGAGGATGAGGAGGAGGAGGAGGAGGAGGCGGTGGCAGTACCTCAGCCCATGC
  CAAGCATCCCGGAGGACCTGGAGAGCCGGGAAGCCATGGTGGCATTTTTCAACTCAGCTGTAGCCAGTGC
  CGAGGAGGAGCAGGCAAGGCTACGTGGGCAGCTGAAGGAGCAAAGGGTGCGCTGCCGGCGCCTGGCTCAC
  CTGCTGGCCTCGGCCCAGAAGGAGCCTGAGGCAGCAGCCCCAGCCCCAGGGACCGGGGGTGATTCTGTGT
  GTGGGGAGACCCACCGGGCCCTGCAGGGGGCCATGGAGAAGCTGCAGAGCCGCTTTATGGAGCTCATGCA
  GGAGAAGGCAGACCTGAAGGAGAGGGTAGAGGAACTGGAACATCGCTGCATCCAGCTTTCTGGAGAGACA
  GACACCATTGGAGAGTACATTGCACTGTACCAGAGCCAGAGGGCAGTGCTGAAGGAGCGGCACCGGGAGA
  AGGAGGAGTACATCAGCAGGCTGGCCCAAGACAAGGAGGAGATGAAGGTGAAGCTGCTGGAGCTGCAGGA
  GCTGGTCTTACGGCTTGTGGGCGACCGCAACGAGTGGCATGGCAGATTCCTGGCAGCTGCCCAGAACCCT
  GCTGATGAGCCCACTTCAGGGGCCCCAGCCCCCCAGGAACTTGGGGCTGCCAACCAGCAGGGTGATCTTT
  GCGAGGTGAGCCTCGCCGGCAGTGTGGAGCCTGCCCAAGGAGAGGCCAGGGAGGGTTCTCCCCGTGACAA
  CCCCACTGCACAGCAGATCATGCAGCTGCTTCGTGAGATGCAGAACCCCCGGGAGCGCCCAGGCTTGGGC
  AGCAACCCCTGCATTCCTTTTTTTTACCGGGCTGACGAGAATGATGAGGTGAAGATCACTGTCATCTAA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_004486
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004486.4, NP_004477.3
• RefSeq Size: 4304 bp
• RefSeq ORF: 3009 bp
• Locus ID: 2801
• Cytogenetics: 9q34.11
• Domains: M
• Gene Summary: 'The Golgi apparatus, which participates in glycosylation and transport of proteins and lipids in the secretory pathway, consists of a series of stacked cisternae (flattened membrane sacs). Interactions between the Golgi and microtubules are thought to be important for the reorganization of the Golgi after it fragments during mitosis. This gene encodes one of the golgins, a family of proteins localized to the Golgi. This encoded protein has been postulated to play roles in the stacking of Golgi cisternae and in vesicular transport. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of these variants has not been determined. [provided by RefSeq, Feb 2010]'