TRPC5 (NM_012471) Human Untagged Clone

CAT#: SC310023

TRPC5 (untagged)-Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRPC5
• Synonyms: PPP1R159; TRP5
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_012471 edited
  GAGGAGTCCGGCAAGGCGCCGCACCACGCATCCTGGCCGGTGGACATCCAGACGACACAG
  ATTTTATCCGAAAAAACCACTTGGCTCAGGATTTCTGCATTGCTATGAACCATGGCCCAA
  CTGTACTACAAAAAGGTCAACTACTCACCGTACAGAGACCGCATCCCCCTGCAAATTGTG
  AGGGCTGAGACAGAGCTCTCTGCAGAGGAGAAGGCCTTCCTCAATGCTGTGGAGAAGGGG
  GACTATGCCACTGTGAAGCAGGCCCTTCAGGAGGCTGAGATCTACTATAATGTTAACATC
  AACTGCATGGACCCCTTGGGCCGGAGTGCCCTGCTCATTGCCATTGAGAACGAGAACCTG
  GAGATCATGGAGCTACTGCTGAACCACAGCGTGTATGTGGGTGATGCATTGCTCTATGCC
  ATACGCAAGGAAGTGGTGGGCGCTGTGGAGCTTCTGCTCAGCTACAGGCGGCCCAGCGGA
  GAGAAGCAGGTCCCCACTCTGATGATGGACACGCAGTTCTCTGAATTCACACCGGACATC
  ACTCCCATCATGCTGGCTGCCCACACCAACAACTACGAAATCATCAAATTGCTTGTCCAA
  AAACGGGTCACTATCCCACGGCCCCACCAGATCCGCTGCAACTGTGTGGAGTGTGTGTCT
  AGTTCAGAGGTAGACAGCCTGCGCCACTCTCGCTCCCGACTGAACATCTATAAGGCTCTG
  GCAAGCCCCTCACTCATTGCCTTATCAAGTGAGGACCCCATCCTAACTGCCTTCCGTCTG
  GGCTGGGAGCTCAAGGAGCTCAGCAAGGTGGAGAATGAGTTCAAGGCCGAGTATGAGGAG
  CTCTCTCAGCAGTGCAAGCTCTTTGCCAAAGACCTGCTGGACCAAGCTCGGAGCTCCAGG
  GAACTGGAGATCATCCTCAACCATCGAGATGACCACAGTGAAGAGCTTGACCCTCAGAAG
  TACCATGACCTGGCCAAGTTGAAGGTGGCAATCAAATACCACCAGAAAGAGTTTGTTGCT
  CAGCCCAACTGCCAACAGTTGCTTGCCACCCTGTGGTATGATGGCTTCCCTGGATGGCGG
  CGGAAACACTGGGTAGTCAAGCTTCTAACCTGCATGACCATTGGGTTCCTGTTTCCCATG
  CTGTCTATAGCCTACCTGATCTCACCCAGGAGCAACCTTGGGCTGTTCATCAAGAAACCC
  TTTATCAAGTTTATCTGCCACACAGCATCCTATTTGACCTTCCTCTTTATGCTTCTCCTG
  GCTTCTCAGCACATTGTCAGGACAGACCTTCATGTACAGGGGCCTCCCCCAACTGTCGTG
  GAATGGATGATATTGCCTTGGGTTCTAGGTTTCATTTGGGGTGAGATTAAGGAAATGTGG
  GATGGTGGATTTACTGAATACATCCATGACTGGTGGAACCTGATGGATTTTGCAATGAAC
  TCCCTCTACCTGGCAACTATTTCCCTGAAGATTGTGGCCTATGTCAAGTATAATGGTTCT
  CGTCCAAGGGAGGAATGGGAAATGTGGCACCCGACTCTGATTGCGGAAGCACTCTTCGCA
  ATATCCAACATTTTAAGTTCGTTGCGTCTCATATCCCTGTTCACAGCCAACTCCCACTTA
  GGACCTCTGCAGATCTCTTTGGGACGCATGCTGCTTGATATCCTCAAATTCCTCTTTATC
  TACTGCCTGGTACTACTAGCTTTTGCCAATGGACTGAACCAGCTTTACTTCTATTATGAA
  ACCAGAGCTATCGATGAGCCTAACAACTGCAAGGGGATCCGATGTGAGAAACAGAACAAT
  GCCTTCTCCACGCTCTTTGAGACTCTTCAGTCACTCTTCTGGTCTGTATTTGGCCTTTTA
  AATCTATATGTCACCAATGTGAAAGCCAGACACGAATTCACCGAGTTTGTAGGAGCTACC
  ATGTTTGGAACATACAATGTCATCTCCCTGGTAGTGCTGCTGAACATGCTGATTGCTATG
  ATGAACAACTCCTATCAGCTTATTGCCGATCATGCTGATATCGAGTGGAAGTTTGCAAGG
  ACGAAGCTCTGGATGAGTTACTTTGATGAAGGTGGCACCTTGCCACCTCCTTTCAACATC
  ATCCCCAGCCCCAAGTCATTTCTATACCTTGGTAACTGGTTCAACAACACCTTCTGCCCC
  AAAAGAGACCCTGACGGTAGACGGAGAAGGCGCAACTTGAGAAGTTTCACAGAACGCAAT
  GCTGACAGCCTGATACAAAATCAACATTATCAGGAAGTTATCAGGAATTTAGTCAAAAGA
  TATGTGGCTGCTATGATAAGAAATTCCAAAACACATGAGGGACTTACAGAAGAAAATTTT
  AAGGAATTAAAGCAAGACATCTCCAGCTTTCGGTATGAAGTGCTTGACCTCTTGGGAAAT
  AGAAAACATCCAAGGAGCTTTTCCACTAGCAGCACTGAACTGTCTCAGAGAGACGATAAT
  AATGATGGCAGTGGTGGGGCTCGGGCCAAATCCAAGAGTGTCTCTTTTAATTTAGGCTGC
  AAGAAAAAGACTTGCCATGGGCCACCTCTCATCAGAACCATGCCAAGGTCCAGTGGTGCC
  CAAGGAAAGTCAAAAGCTGAGTCATCAAGCAAACGCTCCTTCATGGGTCCTTCTCTCAAG
  AAACTGGGTCTCCTATTCTCCAAATTTAATGGTCATATGTCTGAACCCAGTTCAGAGCCA
  ATGTACACAATTTCTGATGGAATTGTTCAGCAGCACTGTATGTGGCAGGACATCAGATAT
  TCTCAGATGGAGAAAGGGAAAGCAGAGGCCTGTTCTCAAAGTGAAATTAACCTCAGTGAG
  GTAGAATTAGGTGAAGTCCAGGGCGCTGCTCAGAGCAGTGAATGCCCTCTAGCCTGTTCC
  AGCTCTCTTCACTGTGCATCCAGCATCTGCTCCTCAAATTCTAAACTTTTAGACTCCTCA
  GAGGATGTATTTGAAACTTGGGGAGAGGCTTGTGACTTGCTCATGCACAAATGGGGTGAT
  GGACAGGAAGAACAAGTTACAACTCGCCTCTAAGTCAAGCCCTAATCATCTGTTCTGGAA
  CTCAACAGAAATCTGTAATTTCCTTGCTCTCAGAGGTGACACAGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_012471 unedited
  NAGTCGTAATTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGGAGGAGTC
  CGGCAAGGCGCCGCACCACGCATCCTGGCCGGTGGACATCCAGACGACACAGATTTTATC
  CGAAAAAACCACTTGGCTCAGGATTTCTGCATTGCTATGAACCATGGCCCAACTGTACTA
  CAAAAAGGTCAACTACTCACCGTACAGAGACCGCATCCCCCTGCAAATTGTGAGGGCTGA
  GACAGAGCTCTCTGCAGAGGAGAAGGCCTTCCTCAATGCTGTGGAGAAGGGGGACTATGC
  CACTGTGAAGCAGGCCCTTCAGGAGGCTGAGATCTACTATAATGTTAACATCAACTGCAT
  GGACCCCTTGGGCCGGAGTGCCCTGCTCATTGCCATTGAGAACGAGAACCTGGAGATCAT
  GGAGCTACTGCTGAACCACAGCGTGTATGTGGGTGATGCATTGCTCTATGCCATACGCAA
  GGAAGTGGTGGGCGCTGTGGAGCTTCTGCTCAGCTACAGGCGGCCCAGCGGAGAGAAGCA
  GGTCCCCACTCTGATGATGGACACGCAGTTCTCTGAATTCACACCGGACATCACTCCCAT
  CATGCTGGCTGCCCACACCAACAACTACGAAATCATCAAATTGCTTGTCCAAAAACGGGT
  CACTATCCCACGGCCCCACCAGATCCGCTGNCACTGTGTGGAGTGTGTGTCTAGTTCAGA
  GGTAGACAGCCTGCGCCACTCTCGCTTCCGACTGGACATCTATTAGGCTCTGGNCAGCCC
  CTCACTCATTGCCTTATCAAGTGAGGACCCCATCCTAACTGCCTTNCGTCTGGGCTGGGA
  GCTCAAGGAGCTCAGCAAGGTGGAGAATGAGTTCAAGGCCGAGTATGAGGAGCTCTCTCA
  G
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_012471 unedited
  CCATGGNGATGGCACTTCCAGGTCCAGNANAGCACTGGGGCAGGGTCACAGGGATGCCAC
  CCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGATGCATGCTCGAGCG
  GCCGCCAGTGTGATGGATATCTGCAGAATTCGCCCTTGAGATCTAGATCTGTGTCACCTC
  TGAGAGCAAGGAAATTACAGATTTCTGTTGAGTTCCAGAACAGATGATTAGGGCTTGACT
  TAGAGGCGAGTTGTAACTTGTTCTTCCTGTCCATCACCCCATTTGTGCATGAGCAAGTCA
  CAAGCCTCTCCCCAAGTTTCAAATACATCCTCTGAGGAGTCTAAAAGTTTAGAATTTGAG
  GAGCAGATGCTGGATGCACAGTGAAGAGAGCTGGAACAGGCTAGAGGGCATTCACTGCTC
  TGAGCAGCGCCCTGGACTTCACCTAATTCTACCTCACTGAGGTTAATTTCACTTTGAGAA
  CAGGCCTCTGCTTTCCCTTTCTCCATCTGAGAATATCTGATGTCCTGCCACATACAGTGC
  TGCTGAACAATTCCATCAGAAATTGTGTACATTGGCTCTGAACTGGGTTCAGACATATGA
  CCATTAAATTTGGAGAATAGGAGACCCAGTTTCTTGAGAGAAGGACCCATGAAGGAGCGT
  TTGCTTGATGACTCAGCTTTTGACTTTCCTTGGGCACCACTGGACCTTGGCATGGTTCTG
  ATGAGAGGTGGCCCCATGGCAGTCTTTTTCTTGCAGCCTAAATTAAAAGAGACACTCTTG
  GATTTGGCCCGAGCCCCACCACTGCCATCATTATTATCGTCTCTCTGAGACAGTTCAGTG
  CTGCTAGTGGAAAGCNTCTTGGGATGTTTCTATTTCCCCAGGAGTCAAGCACNTCATACC
  GAAAGCT
  
• Restriction Sites: Please inquire
• ACCN: NM_012471
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_012471.1, NP_036603.1
• RefSeq Size: 5839 bp
• RefSeq ORF: 2922 bp
• Locus ID: 7224
• Cytogenetics: Xq23
• Domains: ANK, ion_trans
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: 'This gene belongs to the transient receptor family. It encodes one of the seven mammalian TRPC (transient receptor potential channel) proteins. The encoded protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. It also interacts with multiple proteins including calmodulin, CABP1, enkurin, Na(+)-H+ exchange regulatory factor (NHERF ), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1). [provided by RefSeq, May 2010]'