TRPC5 (NM_012471) Human Untagged Clone

CAT#: SC310023

TRPC5 (untagged)-Human transient receptor potential cation channel, subfamily C, member 5 (TRPC5)


  "NM_012471" in other vectors (6)

Reconstitution Protocol

USD 760.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol TRPC5
Synonyms PPP1R159; TRP5
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_012471 edited
GAGGAGTCCGGCAAGGCGCCGCACCACGCATCCTGGCCGGTGGACATCCAGACGACACAG
ATTTTATCCGAAAAAACCACTTGGCTCAGGATTTCTGCATTGCTATGAACCATGGCCCAA
CTGTACTACAAAAAGGTCAACTACTCACCGTACAGAGACCGCATCCCCCTGCAAATTGTG
AGGGCTGAGACAGAGCTCTCTGCAGAGGAGAAGGCCTTCCTCAATGCTGTGGAGAAGGGG
GACTATGCCACTGTGAAGCAGGCCCTTCAGGAGGCTGAGATCTACTATAATGTTAACATC
AACTGCATGGACCCCTTGGGCCGGAGTGCCCTGCTCATTGCCATTGAGAACGAGAACCTG
GAGATCATGGAGCTACTGCTGAACCACAGCGTGTATGTGGGTGATGCATTGCTCTATGCC
ATACGCAAGGAAGTGGTGGGCGCTGTGGAGCTTCTGCTCAGCTACAGGCGGCCCAGCGGA
GAGAAGCAGGTCCCCACTCTGATGATGGACACGCAGTTCTCTGAATTCACACCGGACATC
ACTCCCATCATGCTGGCTGCCCACACCAACAACTACGAAATCATCAAATTGCTTGTCCAA
AAACGGGTCACTATCCCACGGCCCCACCAGATCCGCTGCAACTGTGTGGAGTGTGTGTCT
AGTTCAGAGGTAGACAGCCTGCGCCACTCTCGCTCCCGACTGAACATCTATAAGGCTCTG
GCAAGCCCCTCACTCATTGCCTTATCAAGTGAGGACCCCATCCTAACTGCCTTCCGTCTG
GGCTGGGAGCTCAAGGAGCTCAGCAAGGTGGAGAATGAGTTCAAGGCCGAGTATGAGGAG
CTCTCTCAGCAGTGCAAGCTCTTTGCCAAAGACCTGCTGGACCAAGCTCGGAGCTCCAGG
GAACTGGAGATCATCCTCAACCATCGAGATGACCACAGTGAAGAGCTTGACCCTCAGAAG
TACCATGACCTGGCCAAGTTGAAGGTGGCAATCAAATACCACCAGAAAGAGTTTGTTGCT
CAGCCCAACTGCCAACAGTTGCTTGCCACCCTGTGGTATGATGGCTTCCCTGGATGGCGG
CGGAAACACTGGGTAGTCAAGCTTCTAACCTGCATGACCATTGGGTTCCTGTTTCCCATG
CTGTCTATAGCCTACCTGATCTCACCCAGGAGCAACCTTGGGCTGTTCATCAAGAAACCC
TTTATCAAGTTTATCTGCCACACAGCATCCTATTTGACCTTCCTCTTTATGCTTCTCCTG
GCTTCTCAGCACATTGTCAGGACAGACCTTCATGTACAGGGGCCTCCCCCAACTGTCGTG
GAATGGATGATATTGCCTTGGGTTCTAGGTTTCATTTGGGGTGAGATTAAGGAAATGTGG
GATGGTGGATTTACTGAATACATCCATGACTGGTGGAACCTGATGGATTTTGCAATGAAC
TCCCTCTACCTGGCAACTATTTCCCTGAAGATTGTGGCCTATGTCAAGTATAATGGTTCT
CGTCCAAGGGAGGAATGGGAAATGTGGCACCCGACTCTGATTGCGGAAGCACTCTTCGCA
ATATCCAACATTTTAAGTTCGTTGCGTCTCATATCCCTGTTCACAGCCAACTCCCACTTA
GGACCTCTGCAGATCTCTTTGGGACGCATGCTGCTTGATATCCTCAAATTCCTCTTTATC
TACTGCCTGGTACTACTAGCTTTTGCCAATGGACTGAACCAGCTTTACTTCTATTATGAA
ACCAGAGCTATCGATGAGCCTAACAACTGCAAGGGGATCCGATGTGAGAAACAGAACAAT
GCCTTCTCCACGCTCTTTGAGACTCTTCAGTCACTCTTCTGGTCTGTATTTGGCCTTTTA
AATCTATATGTCACCAATGTGAAAGCCAGACACGAATTCACCGAGTTTGTAGGAGCTACC
ATGTTTGGAACATACAATGTCATCTCCCTGGTAGTGCTGCTGAACATGCTGATTGCTATG
ATGAACAACTCCTATCAGCTTATTGCCGATCATGCTGATATCGAGTGGAAGTTTGCAAGG
ACGAAGCTCTGGATGAGTTACTTTGATGAAGGTGGCACCTTGCCACCTCCTTTCAACATC
ATCCCCAGCCCCAAGTCATTTCTATACCTTGGTAACTGGTTCAACAACACCTTCTGCCCC
AAAAGAGACCCTGACGGTAGACGGAGAAGGCGCAACTTGAGAAGTTTCACAGAACGCAAT
GCTGACAGCCTGATACAAAATCAACATTATCAGGAAGTTATCAGGAATTTAGTCAAAAGA
TATGTGGCTGCTATGATAAGAAATTCCAAAACACATGAGGGACTTACAGAAGAAAATTTT
AAGGAATTAAAGCAAGACATCTCCAGCTTTCGGTATGAAGTGCTTGACCTCTTGGGAAAT
AGAAAACATCCAAGGAGCTTTTCCACTAGCAGCACTGAACTGTCTCAGAGAGACGATAAT
AATGATGGCAGTGGTGGGGCTCGGGCCAAATCCAAGAGTGTCTCTTTTAATTTAGGCTGC
AAGAAAAAGACTTGCCATGGGCCACCTCTCATCAGAACCATGCCAAGGTCCAGTGGTGCC
CAAGGAAAGTCAAAAGCTGAGTCATCAAGCAAACGCTCCTTCATGGGTCCTTCTCTCAAG
AAACTGGGTCTCCTATTCTCCAAATTTAATGGTCATATGTCTGAACCCAGTTCAGAGCCA
ATGTACACAATTTCTGATGGAATTGTTCAGCAGCACTGTATGTGGCAGGACATCAGATAT
TCTCAGATGGAGAAAGGGAAAGCAGAGGCCTGTTCTCAAAGTGAAATTAACCTCAGTGAG
GTAGAATTAGGTGAAGTCCAGGGCGCTGCTCAGAGCAGTGAATGCCCTCTAGCCTGTTCC
AGCTCTCTTCACTGTGCATCCAGCATCTGCTCCTCAAATTCTAAACTTTTAGACTCCTCA
GAGGATGTATTTGAAACTTGGGGAGAGGCTTGTGACTTGCTCATGCACAAATGGGGTGAT
GGACAGGAAGAACAAGTTACAACTCGCCTCTAAGTCAAGCCCTAATCATCTGTTCTGGAA
CTCAACAGAAATCTGTAATTTCCTTGCTCTCAGAGGTGACACAGA
>OriGene 5' read for NM_012471 unedited
NAGTCGTAATTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGGAGGAGTC
CGGCAAGGCGCCGCACCACGCATCCTGGCCGGTGGACATCCAGACGACACAGATTTTATC
CGAAAAAACCACTTGGCTCAGGATTTCTGCATTGCTATGAACCATGGCCCAACTGTACTA
CAAAAAGGTCAACTACTCACCGTACAGAGACCGCATCCCCCTGCAAATTGTGAGGGCTGA
GACAGAGCTCTCTGCAGAGGAGAAGGCCTTCCTCAATGCTGTGGAGAAGGGGGACTATGC
CACTGTGAAGCAGGCCCTTCAGGAGGCTGAGATCTACTATAATGTTAACATCAACTGCAT
GGACCCCTTGGGCCGGAGTGCCCTGCTCATTGCCATTGAGAACGAGAACCTGGAGATCAT
GGAGCTACTGCTGAACCACAGCGTGTATGTGGGTGATGCATTGCTCTATGCCATACGCAA
GGAAGTGGTGGGCGCTGTGGAGCTTCTGCTCAGCTACAGGCGGCCCAGCGGAGAGAAGCA
GGTCCCCACTCTGATGATGGACACGCAGTTCTCTGAATTCACACCGGACATCACTCCCAT
CATGCTGGCTGCCCACACCAACAACTACGAAATCATCAAATTGCTTGTCCAAAAACGGGT
CACTATCCCACGGCCCCACCAGATCCGCTGNCACTGTGTGGAGTGTGTGTCTAGTTCAGA
GGTAGACAGCCTGCGCCACTCTCGCTTCCGACTGGACATCTATTAGGCTCTGGNCAGCCC
CTCACTCATTGCCTTATCAAGTGAGGACCCCATCCTAACTGCCTTNCGTCTGGGCTGGGA
GCTCAAGGAGCTCAGCAAGGTGGAGAATGAGTTCAAGGCCGAGTATGAGGAGCTCTCTCA
G
>OriGene 3' read for NM_012471 unedited
CCATGGNGATGGCACTTCCAGGTCCAGNANAGCACTGGGGCAGGGTCACAGGGATGCCAC
CCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGATGCATGCTCGAGCG
GCCGCCAGTGTGATGGATATCTGCAGAATTCGCCCTTGAGATCTAGATCTGTGTCACCTC
TGAGAGCAAGGAAATTACAGATTTCTGTTGAGTTCCAGAACAGATGATTAGGGCTTGACT
TAGAGGCGAGTTGTAACTTGTTCTTCCTGTCCATCACCCCATTTGTGCATGAGCAAGTCA
CAAGCCTCTCCCCAAGTTTCAAATACATCCTCTGAGGAGTCTAAAAGTTTAGAATTTGAG
GAGCAGATGCTGGATGCACAGTGAAGAGAGCTGGAACAGGCTAGAGGGCATTCACTGCTC
TGAGCAGCGCCCTGGACTTCACCTAATTCTACCTCACTGAGGTTAATTTCACTTTGAGAA
CAGGCCTCTGCTTTCCCTTTCTCCATCTGAGAATATCTGATGTCCTGCCACATACAGTGC
TGCTGAACAATTCCATCAGAAATTGTGTACATTGGCTCTGAACTGGGTTCAGACATATGA
CCATTAAATTTGGAGAATAGGAGACCCAGTTTCTTGAGAGAAGGACCCATGAAGGAGCGT
TTGCTTGATGACTCAGCTTTTGACTTTCCTTGGGCACCACTGGACCTTGGCATGGTTCTG
ATGAGAGGTGGCCCCATGGCAGTCTTTTTCTTGCAGCCTAAATTAAAAGAGACACTCTTG
GATTTGGCCCGAGCCCCACCACTGCCATCATTATTATCGTCTCTCTGAGACAGTTCAGTG
CTGCTAGTGGAAAGCNTCTTGGGATGTTTCTATTTCCCCAGGAGTCAAGCACNTCATACC
GAAAGCT
Restriction Sites Please inquire     
ACCN NM_012471
Insert Size 3100 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_012471.1, NP_036603.1
RefSeq Size 5839 bp
RefSeq ORF 2922 bp
Locus ID 7224
Cytogenetics Xq23
Domains ANK, ion_trans
Protein Families Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
Gene Summary 'This gene belongs to the transient receptor family. It encodes one of the seven mammalian TRPC (transient receptor potential channel) proteins. The encoded protein is a multi-pass membrane protein and is thought to form a receptor-activated non-selective calcium permeant cation channel. The protein is active alone or as a heteromultimeric assembly with TRPC1, TRPC3, and TRPC4. It also interacts with multiple proteins including calmodulin, CABP1, enkurin, Na(+)-H+ exchange regulatory factor (NHERF ), interferon-induced GTP-binding protein (MX1), ring finger protein 24 (RNF24), and SEC14 domain and spectrin repeat-containing protein 1 (SESTD1). [provided by RefSeq, May 2010]'

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