NEDD4 (NM_006154) Human Untagged Clone

CAT#: SC310054

NEDD4 (untagged)-Human neural precursor cell expressed, developmentally down-regulated 4 (NEDD4), transcript variant 1


  "NM_006154" in other vectors (6)

Reconstitution Protocol

SC310054 is the updated version of SC107811.

USD 760.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NEDD4
Synonyms NEDD4-1; RPF1
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_006154 edited
CTCCCGCTTCTCCTCCTCCTCCTCCACAGTTGCCTGCCCTGGGCGGGGGCGAGCGCGTCC
GGTTTGCTGGAAGCGTTCGGAAATGGCAACTTGCGCGGTGGAGGTGTTCGGGCTCCTGGA
GGACGAGGAAAATTCACGAATTGTGAGAGTAAGAGTTATAGCCGGAATAGGCCTTGCCAA
GAAGGATATATTGGGAGCTAGTGATCCTTACGTGAGAGTGACGTTATATGACCCAATGAA
TGGAGTTCTTACAAGTGTGCAAACAAAAACCATTAAAAAGAGTTTGAATCCAAAGTGGAA
TGAAGAAATATTATTCAGAGTTCATCCTCAGCAGCACCGGCTTCTTTTTGAAGTGTTTGA
CGAAAACCGATTGACAAGAGATGATTTCCTAGGTCAAGTGGATGTTCCACTTTATCCATT
ACCGACAGAAAATCCAAGATTGGAGAGACCATATACATTTAAGGATTTTGTTCTTCATCC
AAGAAGTCACAAATCAAGAGTTAAAGGTTATCTGAGACTAAAAATGACTTATTTACCTAA
AACCAGTGGCTCAGAAGATGATAATGCAGAACAGGCTGAGGAATTAGAGCCTGGCTGGGT
TGTTTTGGACCAACCAGATGCTGCTTGCCATTTGCAGCAACAACAAGAACCTTCTCCTCT
ACCTCCAGGGTGGGAAGAGAGGCAGGATATCCTTGGAAGGACCTATTATGTAAACCATGA
ATCTAGAAGAACACAGTGGAAAAGACCAACCCCTCAGGACAACCTAACAGATGCTGAGAA
TGGCAACATTCAACTGCAAGCACAACGTGCATTTACCACCAGGCGGCAGATATCCGAGGA
AACAGAAAGTGTTGACAACCAAGAGTCTTCCGAGAACTGGGAAATTATAAGAGAAGATGA
AGCCACCATGTATAGCAGCCAGGCCTTCCCATCACCTCCACCGTCAAGTAACTTGGATGT
TCCAACTCATCTTGCAGAAGAATTGAATGCCAGACTCACCATTTTTGGAAATTCAGCCGT
GAGCCAGCCAGCATCGAGCTCAAATCATTCCAGCAGAAGAGGCAGCTTACAAGCCTATAC
TTTTGAGGAACAACCTACACTTCCTGTGCTTTTGCCTACTTCATCTGGATTACCACCAGG
TTGGGAAGAAAAACAAGATGAAAGAGGAAGATCATATTATGTAGATCACAATTCCAGAAC
GACTACTTGGACAAAGCCCACTGTACAGGCCACAGTGGAGACCAGTCAGCTGACCTCAAG
CCAGAGTTCTGCAGGCCCTCAATCACAAGCCTCCACCAGTGATTCAGGCCAGCAGGTGAC
CCAGCCATCTGAAATTGAGCAAGGATTCCTTCCTAAAGGCTGGGAAGTCCGGCATGCACC
AAATGGGAGGCCTTTCTTTATTGACCACAACACTAAAACCACCACCTGGGAAGATCCAAG
ATTGAAAATTCCAGCCCATCTGAGAGGAAAGACATCACTTGATACTTCCAATGATCTAGG
GCCTTTACCTCCAGGATGGGAAGAGAGAACTCACACAGATGGAAGAATCTTCTACATAAA
TCACAATATAAAAAGAACACAATGGGAAGATCCTCGGTTGGAGAATGTAGCAATAACTGG
ACCAGCAGTGCCCTACTCCAGGGATTACAAAAGAAAGTATGAGTTCTTCCGAAGAAAGTT
GAAGAAGCAGAATGACATTCCAAACAAATTTGAAATGAAACTTCGCCGAGCAACTGTTCT
TGAAGACTCTTACCGGAGAATTATGGGTGTCAAGAGAGCAGACTTCCTGAAGGCTCGACT
GTGGATTGAGTTTGATGGTGAAAAGGGATTGGATTATGGAGGAGTTGCCAGAGAATGGTT
CTTCCTGATCTCAAAGGAAATGTTTAACCCTTATTATGGGTTGTTTGAATATTCTGCTAC
GGACAATTATACCCTACAGATAAATCCAAACTCTGGATTGTGTAACGAAGATCACCTCTC
TTACTTCAAGTTTATTGGTCGGGTAGCTGGAATGGCAGTTTATCATGGCAAACTGTTGGA
TGGTTTTTTCATCCGCCCATTTTACAAGATGATGCTTCACAAACCAATAACCCTTCATGA
TATGGAATCTGTGGATAGTGAATATTACAATTCCCTAAGATGGATTCTTGAAAATGACCC
AACAGAATTGGACCTCAGGTTTATCATAGATGAAGAACTTTTTGGACAGACACATCAACA
TGAGCTGAAAAATGGTGGATCAGAAATAGTTGTCACCAATAAGAACAAAAAGGAATATAT
TTATCTTGTAATACAATGGCGATTTGTAAACCGAATCCAGAAGCAAATGGCTGCTTTTAA
AGAGGGATTCTTTGAACTAATACCACAGGATCTCATCAAAATTTTTGATGAAAATGAACT
AGAGCTTCTTATGTGTGGACTGGGAGATGTTGATGTGAATGACTGGAGGGAACATACAAA
GTATAAAAATGGCTACAGTGCAAATCATCAGGTTATACAGTGGTTTTGGAAGGCTGTTTT
AATGATGGATTCAGAAAAAAGAATAAGATTACTTCAGTTTGTCACTGGCACATCTCGGGT
GCCTATGAATGGATTTGCTGAACTATACGGTTCAAATGGACCACAGTCATTTACAGTTGA
ACAGTGGGGTACGCCTGAAAAGCTGCCAAGAGCTCATACCTGTTTTAATCGCCTGGACTT
GCCACCTTATGAATCATTTGAAGAATTATGGGATAAACTTCAGATGGCAATTGAAAACAC
CCAGGGCTTTGATGGAGTTGATTAGATTACAAATAACAATCTGTAGTGTTTTTACTGCCA
TAGTTTTATAACCAAAATCTTGACTTAAAATTTTCCGGGGAACTACTAAAATGTGGCCAC
TGAGTCTTCCCAGATCTTGAAGAAAATCATATAAAAAGCATTTGAAGAAATAGTACGACA
ACTTATTTTTAATCACTTTTAAATAATGTGTTGCATTTACACAGTTGTTTCATGCTGTCT
TTAGAGTTAGGTGCCTGCCTAAAGCCAGGCACCACCACACCTGGCTTTAGAGTTCACACA
ATAGGATATAAGTCCTGTATGACTTAAATAGTGAATTTTGTCCTTAACATTTACCTCTTG
TATAGTATCTGCCAGGCAGTTTTTTCTTAAACTACTGAGATGATAACTGTGAAATATTTG
TGATACGTGTCATGTGTGAAAAGTTTGATGCATTTTGAGATGGAAAACTGAAATTTGGAA
AAAGAAATACTTTACTATTGAGTAAACTACAATATATTTAGTGCTACTCGCAGCTATTTA
TTATTTTGTAGACCTGCCTTATGCACCTTACTGCCTAGATTTTTGGGAAAAAACTTTGGA
AAGTGTGTTACCTATATTTCTAGCCAACTAACTCACAGAAAAACTGTTTACTTCTTCACT
TTCGAAGTATTTGGCTTTTGTTAATATGCAGTTTTACTAAACAGATGGTTCATAAGACAT
GTGAAGCAAATTCATATTTGCAATGGGTAAAAAGTATTAAAGCCTTTCTCTTGCCTGCAT
ATCCTATTGACCATTGGTATAGTCATCACTTTTTCATATTTTAGTGTAGTTAGAAGAATT
CCTTCTTCAAACATTAAAGATCCACAAAGCAGTATTTCTAAATATGCCTTGAAGAACTAA
ATGAAGTGTATAGCACTTGCCTTTACTAGATATTCTTTACACTTGTACAATTATGTAGTA
AATGTATGTTTACAGGGTTTATCATGTTTACAGATTAAGCTAATTTCTGTAGTCGCATTT
TTATATTTTTAGTATCACTCTAGTAAAAAAACCAAATAATTTGTTTAAAATAACCAAAGA
GTTGTTATAATGCATAATTTGTATTAAATTTATTACTATTTCTTATGCCTTTTAAAATAC
TGTTTACTATGAAGACAATGTTTTTAATTACAAATCCAGAATTCTGTAGGCAAAATGCTA
CAGTTCATATCTTCCTTTAACCAAACTGAAGTACATAAAGACCATGTACATGTATTCATC
AAACGTTTATTGAATGCTGCGTGCCTAGCGCTGTGCTATGCTCTGGGGTAAGAGTTGTCA
GCTTCAGAGAAGCTGAGTCCTGATCCTCAAGGAACTTGCAAATGTGTCTATGAATTTGTA
AAACAATCAAAAGTAGGCGTAAGCAGAATAAGGCAAAAGGGAAAGTGTTCTAGGTTCCAG
CACACCTGCAAAGATAAAGTGTGCCAAGACTGTATTTATATTTCATACTTATATTGTTTC
ATCCTTATATTGGAATGATTATATAGAAAATGCTCTTAAAAAGATTAAACCTATTTCTCA
GTATGGTATCTTGGTGATTTAGGAATAATTGTAAATATATGTTACGAATCTTCTTAAATA
TATATATATACACACCCTGTGAGAACTGTAAAAAGTACCTCTGGTTCTTGGTTTAAGTTT
GTTGGGGTATAACATGATGAGTACTCATTAGCACCTGATAGAAATCTGAAATGTGACAGT
AGCAAAACCACTTTCTACTTTCCAAACACCACAGCATCAGCATGGTTTAGGGGAAGCAAT
TCACAGATTAATGTACCCTGCGTTTTGTCTTCACCATTGTCAACCAGCAGTCAAGGATGA
GCACCAGGTATAGTCCTGGTTTGATATCTCACCAGCCATGTGAACTGAAGCAGCTTACTT
AACCTCTCTGGATTCATATTTCTTCATCTGTCAAATGAGAGTAATCATGCCCACCCTCAT
TGTCATGGCAGATTGTCAGAAGTTCCAGACAGATGAGAAAGCAAAAAGTACTTTGTAAAG
TGTTATACAATCAGAAGGTGCTTTGTGCTGACCGTCAGATTGTGTCAAGTCAGGTGTGCA
AATTGACCACACTCCCAAAGAATCATTTAAAAGTAAAGTGACTTCTAAGAGACAAGGAAG
TAGGAACATTTCTGATATTAAATATATAAATAATATGGATACACATGTGTATATCTGTAT
ATGGATCTTACCATCATAATTCATTTTCTTCATATCAGCAAAGTAATAGTAATGTGGGCC
AATGCATTTTGGAAATGTCCTCATTATGTAGAATGGAATGTGAAAATTATTTTTGTTAAA
ACAGGATTTTGCCACAATTATTTAAATATTATGTTTGTGAACTATCTAAGCATGAGAAAA
TACAAAGCTTTCTTTGTATCTGGCTGATACTCATTTGGATGATTCTGTGACTCATAGAAC
ATGATGTTAAATGAGACCAGACTCAACTGCCACCAGTCCCCAGCTGCAGACCTTCATCCC
CTTCATCTCCCACTAGGGGCTCGTGGTCTGGAAGAAACATGTATCAAGCAACACAGCCCC
TTATCCCAGATAGAAAAGTGTCTCAAATGCATTCTACTGCGGGACAGTCAGTAGGATCAT
TTTCATAAAGCAAGGACATCATATGTTTCTAGAAATTACAAGCATATAACTTTAGCCTAC
AATCTCTTATTAAAAATTTTTAACAAAATTGTATTACAAATGCATTTCATCAGAACTCAA
ATTTAAATGGTGTTTGTTTTGGGTTTTTATTTATAATGCTGAAGTTATTCCATATAAGTA
TCAAGTTAAACACAATTCATTTTGATTATAAACTATTTGACTTTTTAAAATCTTCTGACA
CAGTAAATATATATATCAAGATTGATGTATCAAAATTTATTGCACACTTTAAAGTGTAAA
ATCATTTTTTAAAATCTTGAATCCACAAATAAAGTTCTATTCTGATTTTAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAA
Restriction Sites Please inquire     
ACCN NM_006154
Insert Size 5900 bp
OTI Disclaimer Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
OTI Annotation The ORF of this clone has been fully sequenced and found to contain 2 SNPs compared with NM_006154.1.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_006154.1, NP_006145.1
RefSeq Size 5749 bp
RefSeq ORF 2703 bp
Locus ID 4734
Cytogenetics 15q21.3
Protein Families Druggable Genome
Protein Pathways Endocytosis, Ubiquitin mediated proteolysis
Gene Summary 'This gene is the founding member of the NEDD4 family of HECT ubiquitin ligases that function in the ubiquitin proteasome system of protein degradation. The encoded protein contains an N-terminal calcium and phospholipid binding C2 domain followed by multiple tryptophan-rich WW domains and, a C-terminal HECT ubiquitin ligase catalytic domain. It plays critical role in the regulation of a number of membrane receptors, endocytic machinery components and the tumor suppressor PTEN. [provided by RefSeq, Jul 2016]'
Transcript Variant: This variant (1) differs in the 5' UTR, lacks a portion of the 5' coding region, and contains multiple alternate 5' coding exons, compared to variant 2. It represents use of an alternate promoter and initiates translation at an alternate start codon. The encoded isoform (1) is shorter and has a distinct N-terminus, compared to isoform 2. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.

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