CLCN3 (NM_173872) Human Untagged Clone

CAT#: SC310074

CLCN3 (untagged)-Human chloride channel 3 (CLCN3), transcript variant e

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLCN3
• Synonyms: ClC-3; CLC3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_173872, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGAGTCTGAGCAGCTGTTCCATAGAGGCTACTATAGAAACAGCTACAACAGTATAACAAGTGCAAGTA
  GTGATGAGGAACTTTTAGATGGAGCAGGTGTTATTATGGACTTTCAAACATCTGAAGATGACAATTTATT
  AGATGGTGACACTGCAGTTGGAACTCATTATACAATGACAAATGGAGGCAGCATTAACAGTTCTACACAT
  TTACTGGATCTTTTGGATGAACCAATTCCAGGTGTTGGTACATATGATGATTTCCATACTATTGATTGGG
  TGCGAGAAAAATGTAAAGACAGAGAAAGGCATAGACGGATCAACAGCAAAAAGAAAGAATCAGCATGGGA
  AATGACAAAAAGTTTGTATGATGCGTGGTCAGGATGGCTAGTAGTAACACTAACAGGATTGGCATCAGGG
  GCACTGGCCGGATTAATAGACATTGCTGCCGATTGGATGACTGACCTAAAGGAGGGCATTTGCCTTAGTG
  CGTTGTGGTACAACCACGAACAGTGCTGTTGGGGATCTAATGAAACAACATTTGAAGAGAGGGATAAATG
  TCCACAGTGGAAAACATGGGCAGAATTAATCATAGGTCAAGCAGAGGGTCCTGGTTCTTATATCATGAAC
  TACATAATGTACATCTTCTGGGCCTTGAGTTTTGCCTTTCTTGCAGTTTCCCTGGTAAAGGTATTTGCTC
  CATATGCCTGTGGCTCTGGAATTCCAGAGATTAAAACTATTTTAAGTGGATTCATCATCAGAGGTTACTT
  GGGAAAATGGACTTTAATGATTAAAACCATCACATTAGTCCTGGCTGTGGCATCAGGTTTGAGTTTAGGA
  AAAGAAGGTCCCCTGGTACATGTTGCCTGTTGCTGCGGAAATATCTTTTCCTACCTCTTTCCAAAGTATA
  GCACAAACGAAGCTAAAAAAAGGGAGGTGCTATCAGCTGCCTCAGCTGCAGGGGTTTCTGTAGCTTTTGG
  TGCACCAATTGGAGGAGTTCTTTTTAGCCTGGAAGAGGTTAGCTATTATTTTCCTCTCAAAACTTTATGG
  AGATCATTTTTTGCTGCTTTAGTGGCTGCATTTGTTTTGAGGTCCATCAATCCATTTGGTAACAGCCGTC
  TGGTCCTTTTTTATGTGGAGTATCATACACCATGGTACCTTTTTGAACTGTTTCCTTTTATTCTTCTAGG
  GGTATTTGGAGGGCTTTGGGGAGCCTTTTTCATTAGGGCAAATATTGCCTGGTGTCGTCGACGCAAGTCC
  ACGAAATTTGGAAAGTATCCCGTTCTGGAAGTCATTATTGTTGCAGCCATTACTGCTGTGATAGCCTTCC
  CTAATCCATACACTAGGCTAAACACCAGTGAACTGATCAAAGAGCTTTTTACAGACTGTGGTCCCCTGGA
  ATCCTCTTCTCTTTGTGACTACAGAAATGACATGAATGCCAGTAAAATTGTCGATGACATTCCTGATCGT
  CCAGCAGGCATTGGAGTATATTCAGCTATATGGCAGTTATGCCTGGCACTCATATTTAAAATCATAATGA
  CAGTATTCACTTTTGGCATCAAGGTTCCATCAGGCTTGTTCATCCCCAGCATGGCCATTGGAGCGATCGC
  AGGAAGGATTGTGGGGATTGCGGTGGAGCAGCTTGCCTACTATCACCACGACTGGTTTATCTTTAAGGAG
  TGGTGTGAGGTCGGGGCTGATTGCATTACACCTGGCCTTTATGCCATGGTTGGTGCTGCTGCATGCTTAG
  GTGGTGTGACAAGAATGACTGTCTCCCTGGTGGTTATTGTTTTTGAGCTTACTGGAGGCTTGGAATATAT
  TGTTCCCCTTATGGCTGCAGTCATGACCAGTAAATGGGTTGGAGATGCCTTTGGCAGGGAAGGCATTTAT
  GAAGCACACATCCGATTAAATGGATACCCTTTCTTGGATGCAAAAGAAGAATTCACTCATACCACCCTGG
  CTGCTGACGTTATGAGACCTCGAAGGAATGATCCTCCCTTAGCTGTCCTGACACAGGACAATATGACAGT
  GGATGATATAGAAAACATGATTAATGAAACCAGCTACAATGGATTTCCTGTCATAATGTCAAAAGAATCT
  CAGAGATTAGTGGGATTTGCCCTCAGAAGAGACCTGACAATTGCAATAGAAAGTGCCAGGAAAAAACAAG
  AAGGTATCGTTGGCAGTTCTCGGGTGTGTTTTGCACAGCACACCCCATCTCTTCCAGCAGAAAGTCCTCG
  GCCATTGAAGCTTCGAAGCATTCTTGACATGAGCCCTTTTACAGTGACAGACCACACCCCAATGGAGATC
  GTGGTGGATATTTTCCGAAAGCTGGGACTGAGGCAGTGCCTTGTAACTCACAATGGGATTGTCTTGGGGA
  TCATCACAAAGAAGAACATATTAGAGCATCTCGAGCAACTAAAGCAGCACGTCGAACCCTTGGCGCCTCC
  TTGGCATTATAACAAAAAAAGATATCCTCCGGCATATGGCCCAGACGGCAAACCAAGACCCCGCTTCAAT
  AATGTTCAACTGAATCTCACAGATGAGGAGAGAGAAGAAACGGAAGAGGAAGTTTATTTGTTGAATAGCA
  CAACTCTTTAA
  
  
• Restriction Sites: AscI-MluI
• ACCN: NM_173872
• ORF Size: 2601 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.

Reference Data

• RefSeq: NM_173872.3, NP_776297.2
• RefSeq Size: 6299
• RefSeq ORF: 2601
• Locus ID: 1182
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Gene Summary: This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (e) has an additional exon in the 3' coding region, which results in frame-shift, compared to variant b. The resulting isoform (e) has a longer and distinct C-terminus, compared to isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.