TRPC3 (NM_003305) Human Untagged Clone

CAT#: SC310083

TRPC3 (untagged)-Human transient receptor potential cation channel, subfamily C, member 3 (TRPC3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRPC3
• Synonyms: SCA41; TRP3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003305 edited
  CGTCTCTCTGTGGTTCAGTCACATTTGGGGTAAAACTGGAACCGAAGCCCTATAGGGTCC
  CCTGAGGGAGAGAGTGGGCATCGGGCAGCCGGGGAACCGCTTTAGTGATAACTTTGACTG
  CGGTTCCCAGGCGGTGCAGGGTCAGCGGCTCGCGCCCCGGCGCGCGGGCTCTCTTTTTCT
  CTTTCTTTCTTTCCCTCTCTCTCTTTCTAAACGAAGGAGCGGTCGGCGCGCTAATGCATG
  AGAAAGAGCAACTTTTCTTAATTTCAATCTTGGAGACAGCTTTTAATCAGTGCGAGACGC
  AGAGAAGATGAAAAGAGGGTGATCGGTTTCACAGGGTCCATGGAGGGAAGCCCATCCCTG
  AGACGCATGACAGTGATGCGGGAGAAGGGCCGGCGCCAGGCTGTCAGGGGCCCGGCCTTC
  ATGTTCAATGACCGCGGCACCAGCCTCACCGCCGAGGAGGAGCGCTTCCTCGACGCCGCC
  GAGTACGGCAACATCCCAGTGGTGCGCAAGATGCTGGAGGAGTCCAAGACGCTGAACGTC
  AACTGCGTGGACTACATGGGCCAGAACGCGCTGCAGCTGGCTGTGGGCAACGAGCACCTG
  GAGGTGACCGAGCTGCTGCTCAAGAAGGAGAACCTGGCGCGCATTGGCGACGCCCTGCTG
  CTCGCCATCAGCAAGGGCTACGTGCGCATCGTAGAGGCCATCCTCAACCACCCTGGCTTC
  GCGGCCAGCAAGCGTCTCACTCTGAGCCCCTGTGAGCAGGAGCTGCAGGACGACGACTTC
  TACGCTTACGACGAGGACGGCACGCGCTTCTCGCCGGACATCACCCCCATCATCCTGGCG
  GCGCACTGCCAGAAATACGAAGTGGTGCACATGCTGCTGATGAAGGGTGCCAGGATCGAG
  CGGCCGCACGACTATTTCTGCAAGTGCGGGGACTGCATGGAGAAGCAGAGGCACGACTCC
  TTCAGCCACTCACGCTCGAGGATCAATGCCTACAAGGGGCTGGCCAGCCCGGCTTACCTC
  TCATTGTCCAGCGAGGACCCGGTGCTTACGGCCCTAGAGCTCAGCAACGAGCTGGCCAAG
  CTGGCCAACATAGAGAAGGAGTTCAAGAATGACTATCGGAAGCTCTCCATGCAATGCAAA
  GACTTTGTAGTGGGTGTGCTGGATCTCTGCCGAGACTCAGAAGAGGTAGAAGCCATTCTG
  AATGGAGATCTGGAATCAGCAGAGCCTCTGGAGGTACACAGGCACAAAGCTTCATTAAGT
  CGTGTCAAACTTGCCATTAAGTATGAAGTCAAAAAGTTTGTGGCTCATCCCAACTGCCAG
  CAGCAGCTCTTGACGATCTGGTATGAGAACCTCTCAGGCCTAAGGGAGCAGACCATAGCT
  ATCAAGTGTCTCGTTGTGCTGGTCGTGGCCCTGGGCCTTCCATTCCTGGCCATTGGCTAC
  TGGATCGCACCTTGCAGCAGGCTGGGGAAAATTCTGCGAAGCCCTTTTATGAAGTTTGTA
  GCACATGCAGCTTCTTTCATCATCTTCCTGGGTCTGCTTGTGTTCAATGCCTCAGACAGG
  TTCGAAGGCATCACCACGCTGCCCAATATCACAGTTACTGACTATCCCAAACAGATCTTC
  AGGGTGAAAACCACCCAGTTTACATGGACTGAAATGCTAATTATGGTCTGGGTTCTTGGA
  ATGATGTGGTCTGAATGTAAAGAGCTCTGGCTGGAAGGACCTAGGGAATACATTTTGCAG
  TTGTGGAATGTGCTTGACTTTGGGATGCTGTCCATCTTCATTGCTGCTTTCACAGCCAGA
  TTCCTAGCTTTCCTTCAGGCAACGAAGGCACAACAGTATGTGGACAGTTACGTCCAAGAG
  AGTGACCTCAGTGAAGTGACACTCCCACCAGAGATACAGTATTTCACTTATGCTAGAGAT
  AAATGGCTCCCTTCTGACCCTCAGATTATATCTGAAGGCCTTTATGCCATAGCTGTTGTG
  CTCAGCTTCTCTCGGATTGCGTACATCCTCCCTGCAAATGAGAGCTTTGGCCCCCTGCAG
  ATCTCTCTTGGAAGGACTGTAAAGGACATATTCAAGTTCATGGTCCTCTTTATTATGGTG
  TTTTTTGCCTTTATGATTGGCATGTTCATACTTTATTCTTACTACCTTGGGGCTAAAGTT
  AATGCTGCTTTTACCACTGTAGAAGAAAGTTTCAAGACTTTATTTTGGTCAATATTTGGG
  TTGTCTGAAGTGACTTCCGTTGTGCTCAAATATGATCACAAATTCATAGAAAATATTGGA
  TACGTTCTTTATGGAATATACAATGTAACTATGGTGGTCGTTTTACTCAACATGCTAATT
  GCTATGATTAATAGCTCATATCAAGAAATTGAGGATGACAGTGATGTAGAATGGAAGTTT
  GCTCGTTCAAAACTTTGGTTATCCTATTTTGATGATGGAAAAACATTACCTCCACCTTTC
  AGTCTAGTTCCTAGTCCAAAATCATTTGTTTATTTCATCATGCGAATTGTTAACTTTCCC
  AAATGCAGAAGGAGAAGGCTTCAGAAGGATATAGAAATGGGAATGGGTAACTCAAAGTCC
  AGGTTAAACCTCTTCACTCAGTCTAACTCAAGAGTTTTTGAATCACACAGTTTTAACAGC
  ATTCTCAATCAGCCAACACGTTATCAGCAGATAATGAAAAGACTTATAAAGCGGTATGTT
  TTGAAAGCACAAGTAGACAAAGAAAATGATGAAGTTAATGAAGGTGAATTAAAAGAAATC
  AAGCAAGATATCTCCAGCCTTCGTTATGAACTTTTGGAAGACAAGAGCCAAGCAACTGAG
  GAATTAGCCATTCTAATTCATAAACTTAGTGAGAAACTGAATCCCAGCATGCTGAGATGT
  GAATGATACAGCAACCTGGATTTGGCTTTGACTATAGCACAAATGTGGGCAATAATATTT
  CTAAGTATGAAATACTTGAAAAACTATGATGTAAATTTTTAGTATTAACTACCTTTATCA
  TGTGAACCTTTAAAAGTTAGCTCTTAATGGTTTTATTGTTTTATCACATGAAAATGCATT
  TTATTTGTCTGCTTTGACATTACAGTGGCATACCATTGTGTTGAAAAGCCCAATATTACT
  ATATTATTGAAACTTTTATTCATTTTAGAGTAAACTCCACATCTTTGCACTACCTGTTTG
  CCTCCAAGAGACTATCAGTTCCTTGGGGACAGGGACCATGTCTTATTCATCTTTGTGTCT
  CCAGCATCTAGTACAGTGCCTGGTATATAGTAGGTGCTCAATAAATGTTGAAACCAACTG
  AACTGCCAACAAAATAAAAATAAAAAGTCTTCACTATGTAGCATACCTTCCCTTGTCCAA
  GTTCTGAAGAGGTTTTTTTTTTTTTTTTTAATAGAAACTGAAGACATTTTACAACCAGCT
  ATGACTTGGTAAGACATTCTTAGAATTTTAGGTGTCACTGATAATCCTAGAACCACTGAG
  CCCCAAGTGAAGAATTTAACAACAAAATGGGTTAATGAAAAATATAATTACATTGTATAT
  TTAAGTTTCATAGAATTATTTAAAACAACACATTAAAGATTTTTCTAAAATATAAAAAAA
  AAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003305 unedited
  GGTACACCTTGTATACGACTCCTATAGGGCGGCCGCGATCACGGCACGAGCCGTCTCTCT
  GTGGTTCAGTCACACCCGGGGTAAAACTGGAACCGAAGCCCTATAGGGTCCCCTGAGGGA
  GAGAGTGGGCATCGGGCAGCCGGGGAACCGCTTTAGTGATAACTTTGACTGCGGTTCCCA
  GGCGGTGCAGGGTCAGCGGCTCGCGCCCCGGCGCGCGGGCTCTCTTTTTCTCTTTCTTTC
  TTTCCCTCTCTCTCTTTCTAAACGAAGGAGCGGTCGGCGCGCTAATGCATGAGAAAGAGC
  AACTTTTCTTAATTTCCAATCTTGGAGACAGCTTTTAATCAGTGCGAGACGCAGAGAAGA
  TGAAAAGAGGGTGATCGGTTTCACAGGGTCCATGGAGGGAAGCCCATCCCTGAGACGCAT
  GACAGTGATGCGGGAGAAGGGCCGGCGCCAGGCTGTCAGGGGCCCGGCCTTCATGTTCAA
  TGACCGCGGCACCAGCCTCACCGCCGAGGAGGAGCGCTTCCTCGACGCCGCCGAGTACGG
  CAACATCCCAGTGGTGCGCAAGATGCTGGAGGAGTCCAAGACGCTGAACGTCAACTGCGT
  GGACTACATGGGCCAGAACGCGCTGCAGCTGGCTGTGGGCAACGAGCACCTGGAGGTGAC
  CGAGCTGCTGCTCAAGAAGGAGAACCTGGCGCGCATTGGCGACGCCCTGCTGCTCGCCAT
  CAGCAAGGGCTACGTGCGCATCGTAGAGGCCATCCTCAACCACCCTGGCTTCGCGGCCAG
  CAAGCGTCTCACTCTGAGCCCCTGTGAGCAGAGCTGCAGACGACGACTTCTACGCTTACG
  ACGAGGACGCACGCGCTCTCGCCGGACATCACCCCCATCATCCTGCGGCGCACTGCAGAA
  TACGAAGTGGTGCACATGCTGCTGATGAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_003305 unedited
  TCCAGGCCAGGAGAGGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGATCTGTTCAGG
  AAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTATAT
  TTTAGAAAAATCTTTAATGTGTTGTTTTAAATAATTCTATGAAACTTAAATATACAATGT
  AATTATATTTTTCATTAACCCATTTTGTTGTTAAATTCTTCACTTGGGGCTCAGTGGTTC
  TAGGATTATCAGTGACACCTAAAATTCTAAGAATGTCTTACCAAGTCATAGCTGGTTGTA
  AAATGTCTTCAGTTTCTATTAAAAAAAAAAAAAAAAACCTCTTCAGAACTTGGACAAGGG
  AAGGTATGCTACATAGTGAAGACTTTTTATTTTTATTTTGTTGGCAGTTCAGTTGGTTTC
  AACATTTATTGAGCACCTACTATATACCAGGCACTGTACTAGATGCTGGAGACACAAAGA
  TGAATAAGACATGGTCCCTGTCCCCAAGGAACTGATAGTCTCTTGGAGGCAAACAGGTAG
  TGCAAAGATGTGGAGTTTACTCTAAAATGAATAAAAGTTTCAATAATATAGTAATATTGG
  GCTTTTCAACACAATGGTATGCCACTGTAATGTCAAAGCAGACAAATAAAATGCATTTTC
  ATGTGATAAAACAATAAAACCATTAAGAGCTAACTTTTAAAGGTTCACA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003305
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003305.1, NP_003296.1
• RefSeq Size: 3448 bp
• RefSeq ORF: 2547 bp
• Locus ID: 7222
• Cytogenetics: 4q27
• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane
• Gene Summary: 'The protein encoded by this gene is a membrane protein that can form a non-selective channel permeable to calcium and other cations. The encoded protein appears to be induced to form channels by a receptor tyrosine kinase-activated phosphatidylinositol second messenger system and also by depletion of intracellular calcium stores. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2011]'
  Transcript Variant: This variant (2) lacks an upstream exon , compared to variant 1, resulting in a different 5' UTR and 5' coding region and translation initiation from an inframe, downstream ATG. The encoded protein (isoform b) has a shorter N-terminus, compared to isoform a.