FYB1 (NM_001465) Human Untagged Clone

CAT#: SC310088

FYB (untagged)-Human FYN binding protein (FYB), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FYB1
• Synonyms: ADAP; FYB; PRO0823; SLAP-130; SLAP130; THC3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001465, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGAAATATAACACGGGGGGCAACCCGACAGAGGATGTCTCAGTCAATAGCCGACCCTTCAGAGTCA
  CAGGGCCAAACTCATCTTCAGGAATACAAGCAAGAAAGAACTTATTCAACAACCAAGGAAATGCCAGCCC
  TCCTGCAGGACCCAGCAATGTACCTAAGTTTGGGTCCCCAAAGCCACCTGTGGCAGTCAAACCTTCTTCT
  GAGGAAAAGCCTGACAAGGAACCCAAGCCCCCGTTTCTAAAGCCCACTGGAGCAGGCCAAAGATTCGGAA
  CACCAGCCAGCTTGACCACCAGAGACCCCGAGGCGAAAGTGGGATTTCTGAAACCTGTAGGCCCCAAGCC
  CATCAACTTGCCCAAAGAAGATTCCAAACCTACATTTCCCTGGCCTCCTGGAAACAAGCCATCTCTTCAC
  AGTGTAAACCAAGACCATGACTTAAAGCCACTAGGCCCGAAATCTGGGCCTACTCCTCCAACCTCAGAAA
  ATGAACAGAAGCAAGCGTTTCCCAAATTGACTGGGGTTAAAGGGAAATTTATGTCAGCATCACAAGATCT
  TGAACCCAAGCCCCTCTTCCCCAAACCCGCCTTTGGCCAGAAGCCGCCCCTAAGTACCGAGAACTCCCAT
  GAAGACGAAAGCCCCATGAAGAATGTGTCTTCATCAAAAGGGTCCCCAGCTCCCCTGGGAGTCAGGTCCA
  AAAGCGGCCCTTTAAAACCAGCAAGGGAAGACTCAGAAAATAAAGACCATGCAGGGGAGATTTCAAGTTT
  GCCCTTTCCTGGAGTGGTTTTGAAACCTGCTGCGAGCAGGGGAGGCCCAGGTCTCTCCAAAAATGGTGAA
  GAAAAAAAGGAAGATAGGAAGATAGATGCTGCTAAGAACACCTTCCAGAGCAAAATAAATCAGGAAGAGT
  TGGCCTCAGGGACTCCTCCTGCCAGGTTCCCTAAGGCCCCTTCTAAGCTGACAGTGGGGGGGCCATGGGG
  CCAAAGTCAGGAAAAGGAAAAGGGAGACAAGAATTCAGCCACCCCGAAACAGAAGCCATTGCCTCCCTTG
  TTTACCTTGGGTCCACCTCCACCAAAACCCAACAGACCACCAAATGTTGACCTGACGAAATTCCACAAAA
  CCTCTTCTGGAAACAGTACTAGCAAAGGCCAGACGTCTTACTCAACAACTTCCCTGCCACCACCTCCACC
  ATCCCATCCGGCCAGCCAACCACCATTGCCAGCATCTCACCCATCACAACCACCAGTCCCAAGCCTACCT
  CCCAGAAACATTAAACCTCCGTTTGACCTAAAAAGCCCTGTCAATGAAGACAATCAAGATGGTGTCACGC
  ACTCTGATGGTGCTGGAAATCTAGATGAGGAACAAGACAGTGAAGGAGAAACATATGAAGACATAGAAGC
  ATCCAAAGAAAGAGAGAAGAAAAGGGAAAAGGAAGAAAAGAAGAGGTTAGAGCTGGAGAAAAAGGAACAG
  AAAGAGAAAGAAAAGAAAGAACAAGAAATAAAGAAGAAATTTAAACTAACAGGCCCTATTCAAGTCATCC
  ATCTTGCAAAAGCTTGTTGTGATGTCAAAGGAGGAAAGAATGAACTGAGCTTCAAGCAAGGAGAGCAAAT
  TGAAATCATCCGCATCACAGACAACCCAGAAGGAAAATGGTTGGGCAGAACAGCAAGGGGTTCATATGGC
  TATATTAAAACAACTGCTGTAGAGATTGACTATGATTCTTTGAAACTGAAAAAAGACTCTCTTGGTGCCC
  CTTCAAGACCTATTGAAGATGACCAAGAAGTATATGATGATGTTGCAGAGCAGGATGATATTAGCAGCCA
  CAGTCAGAGTGGAAGTGGAGGGATATTCCCTCCACCACCAGATGATGACATTTATGATGGGATTGAAGAG
  GAAGATGCTGATGATGGCTCCACACTACAGGTTCAAGAGAAGAGTAATACGTGGTCCTGGGGGATTTTGA
  AGATGTTAAAGGGAAAAGATGACAGAAAGAAAAGTATACGAGAGAAACCTAAAGTCTCTGACTCAGACAA
  TAATGAAGGTTCATCTTTCCCTGCTCCTCCTAAACAATTGGACATGGGAGATGAAGTTTACGATGATGTG
  GATACCTCTGATTTCCCTGTTTCATCAGCAGAGATGAGTCAAGGAACTAATGTTGGAAAAGCTAAGACAG
  AAGAAAAGGACCTTAAGAAGCTAAAAAAGCAGGAAAAAGAAGAAAAAGACTTCAGGAAAAAATTTAAATA
  TGATGGTGAAATTAGAGTCCTATATTCAACTAAAGTTACAACTTCCATAACTTCTAAAAAGTGGGGAACC
  AGAGATCTACAGGTAAAACCTGGTGAATCTCTAGAAGTTATACAAACCACAGATGACACAAAAGTTCTCT
  GCAGAAATGAAGAAGGGAAATATGGTTATGTCCTTCGGAGTTACCTAGCGGACAATGATGGAGAGATCTA
  TGATGATATTGCTGATGGCTGCATCTATGACAATGACTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001465
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001465.4, NP_001456.3
• RefSeq Size: 4858 bp
• RefSeq ORF: 2490 bp
• Locus ID: 2533
• Cytogenetics: 5p13.1
• Domains: SH3
• Protein Families: Druggable Genome
• Gene Summary: 'The protein encoded by this gene is an adapter for the FYN protein and LCP2 signaling cascades in T-cells. The encoded protein is involved in platelet activation and controls the expression of interleukin-2. Three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2011]'
  Transcript Variant: This variant (1) differs in the 5' UTR and uses a downstream start codon compared to variant 3. The resulting isoform (1) has a shorter N-terminus compared to isoform 3. Variants 1 and 4 encode the same isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.