FGFR3 (NM_000142) Human Untagged Clone

CAT#: SC310101

FGFR3 (untagged)-Human fibroblast growth factor receptor 3 (FGFR3), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FGFR3
• Synonyms: ACH; CD333; CEK2; HSFGFR3EX; JTK4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_000142 edited
  GAATTCGGCACGAGGTCGCGGGCAGCTGGCGCCGCGCGGTCCTGCTCTGCCGGTCGCACG
  GACGCACCGGCGGGCCGCCGGCCGGAGGGACGGGGCGGGAGCTGGGCCCGCGGACAGCGA
  GCCGGAGCGGGAGCCGCGCGTAGCGAGCCGGGCTCCGGCGCTCGCCAGTCTCCCGAGCGG
  CGCCCGCCTCCCGCCGGTGCCCGCGCCGGGCCGTGGGGGGCAGCATGCCCGCGCGCGCTG
  CCTGAGGACGCCGCGGCCCCCGCCCCCGCCATGGGCGCCCCTGCCTGCGCCCTCGCGCTC
  TGCGTGGCCGTGGCCATCGTGGCCGGCGCCTCCTCGGAGTCCTTGGGGACGGAGCAGCGC
  GTCGTGGGGCGAGCGGCAGAAGTCCCGGGCCCAGAGCCCGGCCAGCAGGAGCAGTTGGTC
  TTCGGCAGCGGGGATGCTGTGGAGCTGAGCTGTCCCCCGCCCGGGGGTGGTCCCATGGGG
  CCCACTGTCTGGGTCAAGGATGGCACAGGGCTGGTGCCCTCGGAGCGTGTCCTGGTGGGG
  CCCCAGCGGCTGCAGGTGCTGAATGCCTCCCACGAGGACTCCGGGGCCTACAGCTGCCGG
  CAGCGGCTCACGCAGCGCGTACTGTGCCACTTCAGTGTGCGGGTGACAGACGCTCCATCC
  TCGGGAGATGACGAAGACGGGGAGGACGAGGCTGAGGACACAGGTGTGGACACAGGGGCC
  CCTTACTGGACACGGCCCGAGCGGATGGACAAGAAGCTGCTGGCCGTGCCGGCCGCCAAC
  ACCGTCCGCTTCCGCTGCCCAGCCGCTGGCAACCCCACTCCCTCCATCTCCTGGCTGAAG
  AACGGCAGGGAGTTCCGCGGCGAGCACCGCATTGGAGGCATCAAGCTGCGGCATCAGCAG
  TGGAGCCTGGTCATGGAAAGCGTGGTGCCCTCGGACCGCGGCAACTACACCTGCGTCGTG
  GAGAACAAGTTTGGCAGCATCCGGCAGACGTACACGCTGGACGTGCTGGAGCGCTCCCCG
  CACCGGCCCATCCTGCAGGCGGGGCTGCCGGCCAACCAGACGGCGGTGCTGGGCAGCGAC
  GTGGAGTTCCACTGCAAGGTGTACAGTGACGCACAGCCCCACATCCAGTGGCTCAAGCAC
  GTGGAGGTGAATGGCAGCAAGGTGGGCCCGGACGGCACACCCTACGTTACCGTGCTCAAG
  ACGGCGGGCGCTAACACCACCGACAAGGAGCTAGAGGTTCTCTCCTTGCACAACGTCACC
  TTTGAGGACGCCGGGGAGTACACCTGCCTGGCGGGCAATTCTATTGGGTTTTCTCATCAC
  TCTGCGTGGCTGGTGGTGCTGCCAGCCGAGGAGGAGCTGGTGGAGGCTGACGAGGCGGGC
  AGTGTGTATGCAGGCATCCTCAGCTACGGGGTGGGCTTCTTCCTGTTCATCCTGGTGGTG
  GCGGCTGTGACGCTCTGCCGCCTGCGCAGCCCCCCCAAGAAAGGCCTGGGCTCCCCCACC
  GTGCACAAGATCTCCCGCTTCCCGCTCAAGCGACAGGTGTCCCTGGAGTCCAACGCGTCC
  ATGAGCTCCAACACACCACTGGTGCGCATCGCAAGGCTGTCCTCAGGGGAGGGCCCCACG
  CTGGCCAATGTCTCCGAGCTCGAGCTGCCTGCCGACCCCAAATGGGAGCTGTCTCGGGCC
  CGGCTGACCCTGGGCAAGCCCCTTGGGGAGGGCTGCTTCGGCCAGGTGGTCATGGCGGAG
  GCCATCGGCATTGACAAGGACCGGGCCGCCAAGCCTGTCACCGTAGCCGTGAAGATGCTG
  AAAGACGATGCCACTGACAAGGACCTGTCGGACCTGGTGTCTGAGATGGAGATGATGAAG
  ATGATCGGCAAACACAAAAACATCATCAACCTGCTGGGCGCCTGCACGCAGGGCGGGCCC
  CTGTACGTGCTGGTGGAGTACGCGGCCAAGGGTAACCTGCGGGAGTTTCTGCGGGCGCGG
  CGGCCCCCGGGCCTGGACTACTCCTTCGACACCTGCAAGCCGCCCGAGGAGCAGCTCACC
  TTCAAGGACCTGGTGTCCTGTGCCTACCAGGTGGCCCGGGGCATGGAGTACTTGGCCTCC
  CAGAAGTGCATCCACAGGGACCTGGCTGCCCGCAATGTGCTGGTGACCGAGGACAACGTG
  ATGAAGATCGCAGACTTCGGGCTGGCCCGGGACGTGCACAACCTCGACTACTACAAGAAG
  ACAACCAACGGCCGGCTGCCCGTGAAGTGGATGGCGCCTGAGGCCTTGTTTGACCGAGTC
  TACACTCACCAGAGTGACGTCTGGTCCTTTGGGGTCCTGCTCTGGGAGATCTTCACGCTG
  GGGGGCTCCCCGTACCCCGGCATCCCTGTGGAGGAGCTCTTCAAGCTGCTGAAGGAGGGC
  CACCGCATGGACAAGCCCGCCAACTGCACACACGACCTGTACATGATCATGCGGGAGTGC
  TGGCATGCCGCGCCCTCCCAGAGGCCCACCTTCAAGCAGCTGGTGGAGGACCTGGACCGT
  GTCCTTACCGTGACGTCCACCGACGAGTACCTGGACCTGTCGGCGCCTTTCGAGCAGTAC
  TCCCCGGGTGGCCAGGACACCCCCAGCTCCAGCTCCTCAGGGGACGACTCCGTGTTTGCC
  CACGACCTGCTGCCCCCGGCCCCACCCAGCAGTGGGGGCTCGCGGACGTGAAGGGCCACT
  GGTCCCCAACAATGTGAGGGGTCCCTAGCAGCCCACCCTGCTGCTGGTGCACAGCCACTC
  CCCGGCATGAGACTCAGTGCAGATGGAGAGACAGCTACACAGAGCTTTGGTCTGTGTGTG
  TGTGTGTGCGTGTGTGTGTGTGTGTGCACATCCGCGTGTGCCTGTGTGCGTGCGCATCTT
  GCCTCCAGGTGCAGAGGTACCCTGGGTGTCCCCGCTGCTGTGCAACGGTCTCCTGACTGG
  TGCTGCAGCACCGAGGGGCCTTTGTTCTGGGGGGACCCAGTGCAGAATGTAAGTGGGCCC
  ACCCGGTGGGACCCCCGTGGGGCAGGGAGCTGGGCCCGACATGGCTCCGGCCTCTGCCTT
  TGCACCACGGGACATCACAGGGTGGGCCTCGGCCCCTCCCACACCCAAAGCTGAGCCTGC
  AGGGAAGCCCCACATGTCCAGCACCTTGTGCCTGGGGTGTTAGTGGCACCGCCTCCC
  
  5' Read Nucleotide Sequence

  >Reverse primer walk for NM_000142 unedited
  NNNNAACAAAAANNNAANNNNCGAGGAGGGTTGCAGCGGCTGGGCAGCGGAGCGGACGGT
  GTTGGCGGCCGGCACGGCCAGCAGCTTCTTGTCCATCCGCTCGGGCCGTGTCCAGTAAGG
  GGCCCCTGTGTCCACACCTGTGTCCTCAGCCTCGTCCTCCCCGTCTTCGTCATCTCCCGA
  GGATGGAGCGTCTGTCACCCGCACACTGAAGTGGCACAGTACGCGCTGCGTGAGCCGCTG
  CCGGCAGCTGTAGGCCCCGGAGTCCTCGTGGGAGGCATTCAGCACCTGCAGCCGCTGGGG
  CCCCACCAGGACACGCTCCGAGGGCACCAGCCCTGTGCCATCCTTGACCCAGACAGTGGG
  CCCCATGGGACCACCCCCGGGCGGGGGACAGCTCAGCTCCACAGCATCCCCGCTGCCGAA
  GACCAACTGCTCCTGCTGGCCGGGCTCTGGGCCCGGGACTTCTGCCGCTCGCCCCACGAC
  GCGCTGCTCCGTCCCCAAGGACTCCGAGGAGGCGCCGGCCACGATGGCCACGGCCACGCA
  GAGCGCGAGGGCGCAGGCAGGGGCGCCCATGGCGGGGGCGGGGGCCGCGGCGTCCTCAGG
  CAGCGCGCGCGGGCATGCTGCCCCCCACGGCCCGGCGCGGGCACCGGCGGGAGGCGGGCG
  CCGCTCGGGAGACTGGCGAGCGCCGGAGCCCGGCTCGCTACGCGCGGCTCCCGCTCCGGC
  TCGCTGTCCGCGGGCCCAGCTCCCGCCCCGTCCCTCCGGCCGGCGGCCCGCCGGTGCGTC
  CGTGCGACCGGCAGAGCAGGACCGCGCGGCGCCAGCTGCCCGCGACCTCGTGCCGAATTC
  GCGGGCGCCCTATAGTGAGTCGTATTACAAAATTCTGACGGTTCACTAAACAAGCTCTGC
  TTATATAGACCTCCCACCGTACACGCCTACCGCCCATTTGCGTCACCGGGCCGGGGTTAT
  TACGACATTTTGGAAATCCCGTTGATTTTGGTGCCAAACCAAACTCCCATTGACGTTAAT
  GGGGGTGGAGACTTGGAAAATCCCCGTGAGATCAAACCCCTATTCCCCCCCCAATTGGTG
  TACTTGCAAAAACCGCAATCCCCATGGAAAATAGGGAATAACTAAATCGTATAATTGTTC
  CGCCCAAGTAAGGAAAATTCCCCTAAAGGGCCATGTTCCTGGGGCAATAAAACCCAGGGC
  GGGGGCACATTTTCCCTCCATTTGTGACTCTCAAAAGGGGGGGGCGGATATTTGTTAAAT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000142 unedited
  NNNGGCGAAGCATCAGGGCAGGAAGCACTGGGGAGGGGTCACAGGGATGCCACCCGGGAT
  CTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTT
  TTTTTTTTTTTTTTTTTTTTCCAGGTTAGCAACCAGGTGTCTTTATTTTTCGGAAAGTTC
  GTCGCTGGGTTAACAAAATCGCACCTGCCGGTTTGGGTGACACCTCTGGCCACCATGCAC
  TGGGCCCCAAAAAAAGACCACCCTGAGCCATGGCCCTGCAGGCAAGCAAGGGACAGCTGC
  CCAAACTCAGGGCCCAGTAACAGTACAAAACGAACCAACTGAATTCACGGCTTCCCTCCA
  AGCTTTGAAAGGTAGCAGTCCAGGCTATAAAACTCTAAAAGCATTGCGTAAAAAGTGTTA
  AGTCTACAACAAATACATCTTGTAAAAACTCAATAAATTATATATATAGATATATATAAA
  CTTGTAACATCTAATAACATCGGAACCTGCACACAGGGCCGGCCCCTCCCTGGAAACCGT
  CTCCCTGCCTGGGACACACAGCAATTAGAAAAATTTGTATGAAAATACCAGCTTGCTTTG
  AAGTCCAAAAAATAAATCTCCTAAAGAAAAATCCTATAGAAATCTAATTCTCCTGAAAAA
  GGACAAAGGAAATAAAATATCTTCACTGGAATCACCTCCAACTATTAACAACCTTTTAGG
  CTTGGAGGGGATCCTCCAGCTCCGGGGCAGGCTGGACGTACATTCTTGGCCGTCCCTCTA
  TCGGCGGGATAAACCTTCTCGGTTGTCAATAAGTTAAAAATTAAAACTCTCCGCCATGTG
  GCCAGCTAAATGCTGGAAGTGTTGGGGGTGGGGGAACAAAAGTGCCAGGGGCCCATAAAT
  AGCTTTACAATATACAGGGTCAGTTTTAAAACAAAACTGCGGGGCCAGAAAAAGCCCTGG
  TCCTCTGCTTGCAAGGGGTGAGACCCCCTGGGGGCCTCCCTCAAGGCCCCCCAAGCCCCG
  GCCAAGGGCCATGCCTGGCCTCCTGTGCCCCCAGGGGTCGCAAGCCCTCCGTTTGACCAC
  CCTTTTTCCCCTCCAAAAAGGGTAAAAAAANAAAGGGAAAAAAACCANAAACCCCCCTCT
  TTTTTAAAGAAAGGGACCCTAATTTTCGGGAAAAACCTTTTGAAAAAAGGGAAAAGGCCC
  CCCTCTCCGGGACCCCGAATTTCACTCTCTAAGGGCAGGGTGGGGAAAGGGAAACCCGCG
  GAGGGGGGGAGGGGGGCCCCAAACACCCCCGGGAGT
  
• Restriction Sites: Please inquire
• ACCN: NM_000142
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: There are 2 nucleotide differences between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of no amino acids.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000142.2, NP_000133.1
• RefSeq Size: 4304 bp
• RefSeq ORF: 2421 bp
• Locus ID: 2261
• Cytogenetics: 4p16.3
• Domains: pkinase, TyrKc, S_TKc, ig, IGc2, IG
• Protein Families: Druggable Genome, Protein Kinase, Transmembrane
• Protein Pathways: Bladder cancer, Endocytosis, MAPK signaling pathway, Pathways in cancer, Regulation of actin cytoskeleton
• Gene Summary: 'This gene encodes a member of the fibroblast growth factor receptor (FGFR) family, with its amino acid sequence being highly conserved between members and among divergent species. FGFR family members differ from one another in their ligand affinities and tissue distribution. A full-length representative protein would consist of an extracellular region, composed of three immunoglobulin-like domains, a single hydrophobic membrane-spanning segment and a cytoplasmic tyrosine kinase domain. The extracellular portion of the protein interacts with fibroblast growth factors, setting in motion a cascade of downstream signals, ultimately influencing mitogenesis and differentiation. This particular family member binds acidic and basic fibroblast growth hormone and plays a role in bone development and maintenance. Mutations in this gene lead to craniosynostosis and multiple types of skeletal dysplasia. [provided by RefSeq, Aug 2017]'
  Transcript Variant: This variant (1) lacks an alternate coding exon and contains another alternate coding exon compared to variant 3. The resulting isoform (1) has the same N- and C-termini but is shorter compared to isoform 3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.