TLE 1 (TLE1) (NM_005077) Human Untagged Clone

CAT#: SC310123

TLE1 (untagged)-Human transducin-like enhancer of split 1 (E(sp1) homolog, Drosophila) (TLE1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TLE1
• Synonyms: ESG; ESG1; GRG1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005077 edited
  ACCGCTGTGACTACTCTGGACTCGCCCGCAGAGTTTGCCCGCCGGGGAGGGTGGCCGATT
  GGCGGAGCGCACTCCTGCTGTTTTCCCCACCATCTCGTGGATGTCGTCCCTCGGGTGGTG
  AGAGAACTTTGCAGTGGGCTGGAGCGCCCTTTGCGGAGAAGCACACGAAGGTGAAGGAAG
  GAAAGAAAGAAGACCGAGGAAGAAGGAGCTCGGAAGAAGGGTCCGGAGCGGCCGGTCCGG
  CCGTGCAGGGCGAGTGCGCCCGAGGCGCGGCGCCCTGATGCTCCCCGGGCTCGAGGAGCA
  TTGGGCACTTGCGGGACTGCTCTCGGTGGAGTACCNCCGTCGGCCCGGAAGGGGTTTGTG
  CAACCGCGGAGACNACCGAGTGCTGGTCGACGGGGGGGCGTGCCGAGCCGCCTCCCGGCG
  CGCCCTCCGCACTTTCCCCGCCTCGTCATCCGTCGCTCCCCGGCCAGGAGCCTCCGCTGC
  GTCTTCAACCTCGCTCCCCTTTGCGTCCCGGGAGCCTGCGAGCACCCGGCCGAAGGCGCA
  GCCGAATCTTGCGGGAGTCGCCCCGAAAGCGTCGGGTTTGTGTGGGGTTAGCGGGGGCCG
  CCGCGCCACCTGCACCTCGCCCGCCGCCGCCTCGGGGAAAGCCCGAAGAGGAGGCGGACC
  AGGAGAAGAGCAAAGAAAAGCAGTCCGTCTGGATTTGTTTGCCCAGGACTGGCGCCGCGC
  ACGCGGATCGCCGAGGGGAGTGCGGTCGGAGTCACCGCGCCCCCGCCTCCCCGCCCGGGC
  AGCTGAGGCCGGGGGTTGGAGCGCTGCCCCCGCGCACAGTCCCCGAGCGCCCGACGTCTC
  CGCGCAGGTTCTTGAAGCAGCTGGGCCTGGGGCGCCCACTAATGTGGCCCTGAGGGCCGG
  AGCCCGCACCGACGGGAGCGGGAGCCGGAGCAGCTGCGGGCGCCGAGTGGCCGGTGCGCC
  CGGCGGAGCGCGCGTGCGTGGCCAGCGCGCTCCCCGCTTCTGCTTGGCTTTCCGGCTTAA
  TTTTCCTCGGCGGGATTAAAGTTGGAAATTGACCGGAGAATTGAGTTGCCGGGGAACAGA
  GCCCCGGCCGCCGCCAGAGCGATGTTCCCGCAGAGCCGGCACCCGACGCCGCACCAGGCT
  GCAGGCCAGCCCTTCAAGTTCACTATCCCGGAGTCCCTGGACCGGATTAAAGAGGAATTC
  CAGTTCCTGCAGGCGCAGTATCACAGCCTTAAATTGGAATGTGAGAAACTGGCAAGTGAA
  AAGACAGAAATGCAGAGGCACTATGTGATGTATTATGAAATGTCATATGGATTAAACATT
  GAAATGCACAAACAGACTGAAATCGCCAAGAGATTGAATACGATTTGTGCACAAGTCATC
  CCATTTCTGTCTCAGGAACATCAACAACAGGTGGCCCAGGCTGTTGAACGTGCCAAACAG
  GTGACCATGGCAGAATTGAATGCCATCATCGGGCAGCAGCAGTTGCAAGCTCAGCATCTT
  TCTCATGGCCACGGACCCCCAGTTCCCCTTACGCCTCACCCTTCGGGACTTCAGCCTCCT
  GGAATCCCGCCCCTCGGGGGCAGTGCCGGCCTTCTTGCGCTGTCTAGTGCTCTGAGTGGG
  CAGTCTCACTTGGCAATAAAAGATGACAAGAAGCACCACGATGCAGAGCACCACAGAGAC
  AGAGAGCCGGGCACAAGTAATTCCCTCCTGGTCCCAGACAGTCTAAGAGGCACAGATAAA
  CGCAGAAATGGACCTGAATTTTCCAATGACATCAAGAAAAGGAAGGTGGATGATAAGGAC
  TCCAGCCACTATGACAGTGATGGTGACAAAAGCGATGACAACTTAGTTGTGGATGTGTCT
  AATGAGGACCCTTCTTCTCCGCGAGCAAGCCCTGCCCACTCGCCCCGGGAAAATGGAATC
  GACAAAAATCGCCTGCTAAAGAAGGATGCTTCTAGCAGTCCAGCTTCCACGGCCTCCTCG
  GCAAGTTCCACTTCTTTGAAATCCAAAGAAATGAGCTTGCATGAAAAAGCCAGCACGCCT
  GTTCTGAAATCCAGCACACCAACGCCTCGGAGCGACATGCCAACGCCGGGCACCAGCGCC
  ACTCCAGGCCTCCGTCCAGGTCTCGGCAAGCCTCCAGCCATAGACCCCCTCGTTAACCAA
  GCGGCAGCTGGCTTGAGGACACCCCTGGCAGTGCCCGGCCCATATCCTGCTCCTTTTGGG
  ATGGTCCCCCACGCTGGCATGAACGGCGAGCTGACCAGCCCAGGCGCTGCCTACGCCAGT
  TTACACAACATGTCGCCCCAGATGAGCGCCGCAGCCGCCGCGGCCGCCGTGGTGGCCTAC
  GGGCGCTCCCCCATGGTGGGGTTTGATCCTCCCCCTCACATGAGAGTACCTACCATTCCT
  CCAAACCTGGCAGGAATCCCTGGGGGGAAACCTGCATACTCCTTCCACGTTACTGCAGAC
  GGTCAGATGCAGCCTGTCCCTTTTCCCCCCGACGCCCTCATCGGACCCGGAATCCCCCGG
  CATGCTCGCCAGATCAACACCCTCAACCACGGGGAGGTGGTGTGCGCTGTGACCATCAGC
  AACCCCACGAGACACGTGTACACAGGCGGGAAGGGCTGCGTCAAGGTCTGGGACATCAGC
  CACCCTGGCAATAAGAGCCCTGTCTCCCAGCTCGACTGTCTGAACAGAGACAATTATATC
  CGTTCCTGTAAATTGCTACCCGATGGCTGCACTCTCATAGTGGGAGGGGAAGCCAGTACT
  TTGTCCATTTGGGACCTGGCGGCTCCAACCCCGCGCATCAAGGCGGAGCTGACGTCCTCG
  GCCCCCGCCTGCTACGCCCTGGCCATCAGCCCCGATTCCAAGGTCTGCTTCTCATGCTGC
  AGCGACGGCAACATCGCTGTGTGGGATCTGCACAACCAGACACTAGTGAGGCAATTCCAG
  GGCCACACAGACGGAGCCAGCTGTATTGACATTTCTAATGATGGCACCAAGCTCTGGACG
  GGTGGTTTGGACAACACAGTCAGGTCCTGGGACCTGCGCGAGGGGCGGCAGCTGCAGCAG
  CACGACTTCACCTCCCAGATCTTCTCCCTGGGGTACTGCCCCACCGGGGAGTGGCTGGCA
  GTGGGCATGGAGAGCAGCAATGTGGAGGTGCTGCACGTGAACAAGCCTGACAAGTACCAG
  CTGCACCTGCATGAGAGCTGCGTGCTGTCCCTGAAATTTGCTTACTGTGGTAAATGGTTT
  GTGAGTACTGGAAAAGATAACCTCCTCAATGCTTGGCGGACCCCCTATGGAGCCAGCATA
  TTCCAGTCCAAAGAGTCCTCGTCAGTGCTTAGCTGTGACATCTCTGTGGATGATAAGTAC
  ATAGTCACTGGCTCGGGGGACAAGAAGGCTACAGTCTATGAAGTCATCTACTGAAAACAT
  TATGTGGTTTAACGTTTATAGTTGAATTGGGCCAAAATGTTTCGAATTTATAGAAATAGA
  AAAGTTGTAACTTTAAAAGAGAAAAAAAATTACAAACACCTGTTTCCAAACCTTGACAGA
  AAACTACTTTGAGTCTACAAAGAGGAGGCGACAAGTCCATCAGCAGAAAGTCACCTGTCT
  ACATAGACCAAATGGAGCACCAAGGCCAAGCGGACAGAGGGGCCATGGGTTGTAGGATTG
  AGGAACGGAATCTGCCGACTCACATGACAGCCCATTCTTTCTTTCTGGGTGATCTGGGGA
  TCACGCCTTGCCCAAGTGTGAGATTACCTTTCTGTTCCTTGCAGTTCACCTCACTTTCCG
  TCCTTTGTAGAGCAGTGGTGTCTCCAATGAACTTGTTTCCTGGTTTTGCATCTTGTGAAA
  TGTTTTTTTGTATTTTTGTTGAAGGTTAAACATTTGTATAAATTGTAAATATATTTGGTT
  TATTACAGTAAAGGCTTTAGTACCAATAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005077 unedited
  NTGGGNCTTAGCTGCTTGTAACATTGCGGCCTTGGCGCCCACTATGTGCCCCTGAGGCCG
  GAGCCCGCACCGACGGGAGCGGGAGCCGTGACAGCTGCGGGCGCCTAGTGGCCGGTGCTC
  CCGGCGGAGCGCGCGTGCGTGGCCAGCGCGCTCCCCGCTTCTGCTTGGCTTTCCGGCTTA
  ATTTTCCTCGGCGGGATTAAAGTTGGAAATTGACCGGATAATTGTGTTGCCGGGGAACAG
  AGCCCCGGCCGCCGCCAGAGCGATGTTCCCGCAGAGCCGGCTCCCGACGCCGCACCATGC
  TGCAGGCCAGCCCTTCAAGTTCACTATCCTGGATTCCCTGGACCGGATTAAAAGAGAATT
  CCAGTTCCTGCGGGCGCAGTATCACATCCTTTAAATTGGAATGTGAGAAACTGGGCTGTC
  GACAGACTGAACTGCTCAGCCCTATGTGATGGTTTATGAAATGTCATATTGGATTAACCA
  TTGGAATGCACAACTAGACTTTTATCGTCTAGCAGTTTAATCCCATTTTTTTCCCATGTA
  TTCACTTATTTTGTTTTATGAACTTTATAGCCAGGTGGCCCCAGACTATTTTTCGTCCCA
  AGCAGTGTTCCAAGGCTTGATTTGAATCCTTGCTTCGTCCATAGAAAAATTCATATCTAT
  GATTTGATCTCTTTGGTAGTGGCGCCCATTTTTCCTTTTTACACCTATTTCTATGAGAGT
  CTTCTACTCCTATAAGATAAACACTTACATTATGGAGTGATGTATCCAGGNAATATATCT
  AATCGTCTACTATCATTCTCGTTCACCAGCATACTCCCCCTCGCCTGACCCATGTTATAT
  GTCAGGAGCACCCTTTTGTTTGTCCTCTATATATGTGCATGGTTAGTATTAACTACAAAA
  TAACAATCGCATACATACCCAACCAATAAATACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005077 unedited
  TTAGAGTCGAGTTTTTTTTTTTTTTTTTTTTATTGGTACTAAAGCCTTTACTGTAATAAA
  CCAAATATATTTACAATTTATACAAATGTTTAACCTTCAACAAAAATACAAAAAAACATT
  TCACAAGATGCAAAACCAGGAAACAAGTTCATTGGAGACACCACTGCTCTACAAAGGACG
  GAAAGTGAGGTGAACTGCAAGGAACAGAAAGGTAATCTCACACTTGGGCAAGGCGTGATC
  CCCAGATCACCCAGAAAGAAAGAATGGGCTGTCATGTGAGTCGGCAGATTCCGTTCCTCA
  ATCCTACAACCCATGGCCCCTCTGTCCGCTTGGCCTTGGTGCTCCATTTGGTCTATGTAG
  ACAGGTGACTTTCTGCTGATGGACTTGTCGCCTCCTCTTTGTAGACTCAAAGTAGTTTTC
  TGTCAAGGTTTGGAAACAGGCGTTTGTAATTTTTTTTCTCTTTTAAAGTTACAACTTTTC
  TATTTCTATAAATTCGAAACATTTTGGCCCAATTCAACTATAAACGTTAAACCACATAAT
  GTTTTCAGTAGATGACTTCATAGACTGTAGCCTTCTTGTCCCCCGAGCCAGTGACTATGT
  ACTTATCATCCACAGAGATGTCACAGCTAACCACTGACGAGGACTTTTTGAACCTGAATA
  TGCTGGCTCCATAAGGGGTCCCCCCACCATTGAAGACGTTATTCTTTCCAGTCCTCCAAA
  CCATTTACCCCAGAAGGCAATTTCAGGGACAGCCCGCCCTTTCATGCCGGTGCACTGGGA
  CTTCCAAGCTTGTTAACTGCAAAACCTCCAATGGTGTTTTCTGCCAATGCCACCACTCCC
  GGTGGGCATACCCCGTGAAAAACTTGGAGGGATTCTGCTTCCGCCCGGCCCCCTCCCGAG
  GCCCAGACCGGACTGTGTTCAAACAACGTGCAGACTGGGGCCACATTTAAATCCATACAC
  GTGTTCCTTTGCTGCCCCGCTAATTTTTATTTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005077
• Insert Size: 4000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005077.3, NP_005068.2
• RefSeq Size: 3283 bp
• RefSeq ORF: 2313 bp
• Locus ID: 7088
• Cytogenetics: 9q21.32
• Domains: WD40, TLE_N
• Protein Families: Transcription Factors
• Gene Summary: ''
  Transcript Variant: This variant (2) uses an alternate in-frame splice site compared to variant 1. The encoded isoform (2) is shorter than isoform 1.