PDE4D (NM_006203) Human Untagged Clone

CAT#: SC310179

PDE4D (untagged)-Human phosphodiesterase 4D, cAMP-specific (PDE4D), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE4D
• Synonyms: ACRDYS2; DPDE3; HSPDE4D; PDE4DN2; PDE43; STRK1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006203 edited
  ATGCACGTGAATAATTTTCCCTTTAGAAGGCATTCCTGGATATGTTTTGATGTGGACAAT
  GGCACATCTGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCAGGATCCGGGCTAATT
  CTCCAAGCAAATTTTGTCCACAGTCAACGACGGGAGTCCTTCCTGTATCGATCCGACAGC
  GATTATGACCTCTCTCCAAAGTCTATGTCCCGGAACTCCTCCATTGCCAGTGATATACAC
  GGAGATGACTTGATTGTGACTCCATTTGCTCAGGTCTTGGCCAGTCTGCGAACTGTACGA
  AACAACTTTGCTGCATTAACTAATTTGCAAGATCGAGCACCTAGCAAAAGATCACCCATG
  TGCAACCAACCATCCATCAACAAAGCCACCATAACAGAGGAGGCCTACCAGAAACTGGCC
  AGCGAGACCCTGGAGGAGCTGGACTGGTGTCTGGACCAGCTAGAGACCCTACAGACCAGG
  CACTCCGTCAGTGAGATGGCCTCCAACAAGTTTAAAAGGATGCTTAATCGGGAGCTCACC
  CATCTCTCTGAAATGAGTCGGTCTGGAAATCAAGTGTCAGAGTTTATATCAAACACATTC
  TTAGATAAGCAACATGAAGTGGAAATTCCTTCTCCAACTCAGAAGGAAAAGGAGAAAAAG
  AAAAGACCAATGTCTCAGATCAGTGGAGTCAAGAAATTGATGCACAGCTCTAGTCTGACT
  AATTCAAGTATCCCAAGGTTTGGAGTTAAAACTGAACAAGAAGATGTCCTTGCCAAGGAA
  CTAGAAGATGTGAACAAATGGGGTCTTCATGTTTTCAGAATAGCAGAGTTGTCTGGTAAC
  CGGCCCTTGACTGTTATCATGCACACCATTTTTCAGGAACGGGATTTATTAAAAACATTT
  AAAATTCCAGTAGATACTTTAATTACATATCTTATGACTCTCGAAGACCATTACCATGCT
  GATGTGGCCTATCACAACAATATCCATGCTGCAGATGTTGTCCAGTCTACTCATGTGCTA
  TTATCTACACCTGCTTTGGAGGCTGTGTTTACAGATTTGGAGATTCTTGCAGCAATTTTT
  GCCAGTGCAATACATGATGTAGATCATCCTGGTGTGTCCAATCAATTTCTGATCAATACA
  AACTCTGAACTTGCCTTGATGTACAATGATTCCTCAGTCTTAGAGAACCATCATTTGGCT
  GTGGGCTTTAAATTGCTTCAGGAAGAAAACTGTGACATTTTCCAGAATTTGACCAAAAAA
  CAAAGACAATCTTTAAGGAAAATGGTCATTGACATCGTACTTGCAACAGATATGTCAAAA
  CACATGAATCTACTGGCTGATTTGAAGACTATGGTTGAAACTAAGAAAGTGACAAGCTCT
  GGAGTTCTTCTTCTTGATAATTATTCCGATAGGATTCAGGTTCTTCAGAATATGGTGCAC
  TGTGCAGATCTGAGCAACCCAACAAAGCCTCTCCAGCTGTACCGCCAGTGGACGGACCGG
  ATAATGGAGGAGTTCTTCCGCCAAGGAGACCGAGAGAGGGAACGTGGCATGGAGATAAGC
  CCCATGTGTGACAAGCACAATGCTTCCGTGGAAAAATCACAGGTGGGCTTCATAGACTAT
  ATTGTTCATCCCCTCTGGGAGACATGGGCAGACCTCGTCCACCCTGACGCCCAGGATATT
  TTGGACACTTTGGAGGACAATCGTGAATGGTACCAGAGCACAATCCCTCAGAGCCCCTCT
  CCTGCACCTGATGACCCAGAGGAGGGCCGGCAGGGTCAAACTGAGAAATTCCAGTTTGAA
  CTAACTTTAGAGGAAGATGGTGAGTCAGACACGGAAAAGGACAGTGGCAGTCAAGTGGAA
  GAAGACACTAGCTGCAGTGACTCCAAGACTCTTTGTACTCAAGACTCAGAGTCTACTGAA
  ATTCCCCTTGATGAACAGGTTGAAGAGGAGGCAGTAGGGGAAGAAGAGGAAAGCCAGCCT
  GAAGCCTGTGTCATAGATGATCGTTCTCCTGACACGTAA
  
  5' Read Nucleotide Sequence

  >Reverse primer walk for NM_006203 unedited
  CTACATGTAAGGACTCCCGTCTGTGACTGTGGACAAAATTTGCTTGGACAATTAACCCGG
  ATCCTGGGCTGCTCATGGGATCCAAGGGACTCCGTCCCGCAAATGTGCCATTGTCCACAT
  CAAAACATATCCAGGAATGCCTTCTAGAGGGAAAATTATTCACGTGCATCATGTTCCCAT
  ATCTTCTGTCATTAATATTTTTCTTGTCTCCTACGTTACATGTAGTGATTTTTACAGATG
  AATTCCAACTCTCTTGTTTGAGCAACTATCGTGCGCAATTATTGCAACACGTATTGAATC
  CCAAAAAAAAAAAAAAAATCTCCCCCCACCAATAAAAACAAATAGCACTTACAAAAGGCC
  TTCCATGATTTGAAAAAACAAACATCACAGAACACTGCAGGTATGGATCCATCCATTTAG
  GTTCCACTATTTAGTCTACATACTCAAATAACCAGTTCAACTAATAATCCTAACTGGCGG
  CCAATATTGTCCTGGAAAAATCTAACCGCCTTGAGTGTGAATTAATGCATCCTTAACGAA
  GGACTCTGGAATGTGGGAAGAGAGGAGTCAAAAGACAGTTTCTTGATATCCTTGCTAAAC
  AAGTGTTCGCATGGAAATGGAAAACTTCAGGTTGCTTTGCCATGCTCGTCTTTAGCAATG
  AATATTGCTTTCTTCTCTTTCTTAGCACATATTCATGTCTGGTCACTTTAACGCAGTGCT
  ACCGTCTGAGACGTGTCGTACTAAGCCTGTGCTGACGGGCTAGCAACCTGTATCACCAAA
  GCCCACTTCTTTCCCAGATTCAGAATTGCTGGTTCAACTGCTAAGTANG
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_006203 unedited
  GTTCATACTGTTGCATATTGCCCACNAAGCTGCTCAAACAAAGATTGGAATTCATCTGTA
  AAAATCACTACATGTAACGTAGGAGACAAGAAAAATATTAATGACAGAAGATCTGCGAAC
  ATGATGCACGTGAATAATTTTCCCTTTAGAAGGCATTCCTGGATATGTTTTGATGTGGAC
  AATGGCACATCTGCGGGACGGAGTCCCTTGGATCCCATGACCAGCCCCAGATCCGGNNCT
  ATTCTNCNAGCANATTTTGTCCACAGTCAACGACGGNAGTCCCTTCTGTATCGATCCGAC
  AGCGATTATGACCTCTCTCCAAAGTCTATGTCCCNGGACTCCTNCATTTGCAGTGATATA
  CCCGAGAATGACTTGAGTGGGACTCCATTTGCCTCAGGTCTGGGCCATCTTGCAAACTGT
  ACAAAACAACTTTTGCTGCTTAACCTAATTTGCAGATCGGCCCCCTGGCCAAGATCACCC
  TTGTGCCAACCACCCTCCTTTAAACAAAGCCCCCTTAACAAAGAGGCCCTCCCCAAAACG
  GGCCCCCCGAACCCCTGAAAAACCTGACTTGGGTTTTGGCCCCCTTTTGAAACCCTTAAA
  ACAGACCCCTCCTCCGTGGAAAAGGGCCCCCCCCAATTTTTTAAAAGAGGGGTTTTATTG
  GGGGGCCCCCCCCCCCCCTTCTTTAAAAAAAAGGGGGGCCCGGGGGAAAAAAAAGGGGGC
  GGAGATTTTTATTATTACAACCACTTTTTTTTAATAATAACACCCCGAGAGGGGGGGAGA
  AACTCTTTTCTCTCCCCCCCCCCCGGGGGGGGGGGGGGAGAAAAAAAAAAACACCCCTCT
  TTCTCTCTTCTAATTGGGGGTTGGACAAAAAAATTTTTTCGCCCCCCCCTCTTTTTTTT
  
• Restriction Sites: Please inquire
• ACCN: NM_006203
• Insert Size: 7000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006203.3, NP_006194.2
• RefSeq Size: 5876 bp
• RefSeq ORF: 2022 bp
• Locus ID: 5144
• Cytogenetics: 5q11.2-q12.1
• Domains: PDEase
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: 'This gene encodes one of four mammalian counterparts to the fruit fly 'dunce' gene. The encoded protein has 3',5'-cyclic-AMP phosphodiesterase activity and degrades cAMP, which acts as a signal transduction molecule in multiple cell types. This gene uses different promoters to generate multiple alternatively spliced transcript variants that encode functional proteins.[provided by RefSeq, Sep 2009]'
  Transcript Variant: This variant (2) differs in the 5' UTR and coding region, compared to variant 1. Isoform PDE4D3 (also known as isoform 2) is shorter and has a distinct N-terminus, compared to isoform PDE4D4. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.