EPB41 (NM_203342) Human Untagged Clone

CAT#: SC310203

EPB41 (untagged)-Human erythrocyte membrane protein band 4.1 (elliptocytosis 1, RH-linked) (EPB41), transcript variant 4

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EPB41
• Synonyms: 4.1R; EL1; HE
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_203342, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCACTGCAAGGTTTCTTTGTTGGATGACACAGTTTATGAATGTGTTGTGGAGAAACATGCTAAGGGAC
  AAGATTTGCTTAAACGAGTATGTGAGCATCTCAATCTTTTGGAAGAAGACTATTTTGGTCTAGCCATTTG
  GGATAACGCAACCTCTAAGACATGGCTGGATTCCGCCAAAGAAATAAAAAAGCAGGTTCGTGGTGTCCCT
  TGGAATTTTACATTTAATGTAAAGTTTTATCCACCTGACCCAGCACAGTTAACAGAAGACATAACAAGAT
  ATTATTTATGTCTTCAGCTTCGGCAGGACATAGTTGCAGGACGTCTGCCCTGTTCCTTTGCAACCTTAGC
  ATTATTAGGTTCTTACACCATCCAGTCTGAACTGGGAGACTACGACCCAGAACTCCATGGCGTGGATTAT
  GTTAGTGATTTTAAACTGGCCCCGAATCAGACCAAGGAACTTGAAGAGAAGGTCATGGAACTGCATAAGT
  CATACAGGTCCATGACTCCAGCTCAGGCTGACTTGGAGTTTCTTGAGAATGCCAAAAAGTTGTCTATGTA
  TGGAGTTGATCTTCATAAAGCAAAGGACTTGGAAGGAGTAGATATCATCCTAGGTGTCTGCTCTAGTGGC
  CTTCTGGTTTACAAAGATAAGCTGAGAATTAACCGCTTCCCTTGGCCCAAAGTGCTGAAGATTTCTTATA
  AACGTAGTAGCTTTTTCATCAAGATTCGGCCTGGAGAGCAAGAGCAGTATGAAAGTACCATCGGATTCAA
  ACTTCCCAGTTACCGAGCAGCTAAGAAATTATGGAAAGTCTGTGTAGAACATCACACGTTTTTCAGATTG
  ACATCTACAGACACCATTCCCAAAAGCAAATTTCTTGCGCTAGGATCCAAATTTCGATACAGTGGCCGGA
  CTCAAGCTCAGACCAGGCAAGCTAGTGCTCTAATTGACAGGCCTGCCCCACACTTCGAGCGTACAGCAAG
  TAAACGGGCGTCCCGGAGCCTCGATGGAGCAGCAGCTGTCGATTCGGCAGACCGAAGTCCTCGGCCCACT
  TCTGCACCTGCCATTACTCAGGGTCAGGTTGCAGAAGGTGGCGTCCTAGATGCCTCTGCTAAAAAAACAG
  TGGTCCCTAAAGCACAGAAGGAAACAGTGAAGGCTGAAGTGAAAAAGGAAGACGAGCCACCTGAGCAAGC
  TGAGCCAGAGCCCACAGAAGCATGGAAGGTGGAAAAAACCCACATCGAGGTGACAGTACCCACCTCAAAT
  GGTGACCAAACACAGAAAAAGAGAGAAAGACTAGATGGTGAAAACATTTATATCAGACATAGCAATTTAA
  TGTTGGAGGATTTAGACAAGAGTCAAGAGGAGATCAAAAAACATCATGCCAGCATCAGTGAGCTGAAAAA
  GAACTTCATGGAGTCTGTACCAGAACCACGGCCTAGTGAATGGGATAAACGCTTATCCACTCACTCACCC
  TTCCGAACTCTTAACATCAATGGGCAAATCCCCACAGGAGAAGGACCTCCCCTGGTGAAGACACAAACTG
  TCACCATCTCAGATAATGCCAATGCTGTGAAAAGTGAAATCCCAACCAAAGACGTCCCTATTGTCCACAC
  TGAGACCAAGACCATCACTTATGAGGCTGCCCAGACTGACGACAACAGTGGAGACTTGGACCCAGGAGTC
  TTGCTGACAGCTCAAACTATCACATCTGAGACCCCAAGCAGCACCACCACAACTCAAATTACCAAGACTG
  TAAAAGGTGGGATTTCAGAGACACGTATTGAAAAGAGAATTGTGATCACAGGAGATGCTGATATTGACCA
  TGATCAGGTCCTTGTACAAGCCATCAAGGAGGCAAAGGAGCAGCACCCAGACATGTCAGTGACCAAGGTG
  GTCGTCCACCAGGAGACCGAGATTGCTGATGAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_203342
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203342.2, NP_976217.1
• RefSeq Size: 5999 bp
• RefSeq ORF: 1926 bp
• Locus ID: 2035
• Cytogenetics: 1p35.3
• Protein Families: Druggable Genome
• Protein Pathways: Tight junction
• Gene Summary: 'The protein encoded by this gene, together with spectrin and actin, constitute the red cell membrane cytoskeletal network. This complex plays a critical role in erythrocyte shape and deformability. Mutations in this gene are associated with type 1 elliptocytosis (EL1). Alternatively spliced transcript variants encoding different isoforms have been described for this gene.[provided by RefSeq, Oct 2009]'
  Transcript Variant: This variant (4) contains an additional exon at the 5' end, and is missing an internal, in-frame coding exon compared to variant 1. This results in translation start from a downstream AUG and a shorter isoform (4) compared to isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.