MEF2A (NM_005587) Human Untagged Clone

CAT#: SC310307

MEF2A (untagged)-Human myocyte enhancer factor 2A (MEF2A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MEF2A
• Synonyms: ADCAD1; mef2; RSRFC4; RSRFC9
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005587 edited
  CCGGGATATCGTCGACCCACGCGTCCGGTCCCGGCGAGTCCCGGGCTGAAAGAGGCGGCT
  CCGGGCGGCGCGAAGCGCTGGTGGCGGGCCCGGGCTGCGGCGTGTGCGCGCCCGCCAGCT
  GCTCCGGAGATACGGAATTGCATTTTGTGAAAAAAGAACAAGAATTTTCTGCAAGGATCA
  TATCTAAGTGCACTTTTTGCTGATACTTCATTTCTAATCTTGTAGAAAATTTCAGCTGTA
  GCCCTTGGACTAGAAGCTGAAATAACAGAAGCTGTGTACGATGCATTAGGGTATTGAAGA
  AAATTAACTTTTGAATTAAATATTTGGAATATAAGGAAATAAGGAAAGTTGACTGAAAAT
  GGGGCGGAAGAAAATACAAATCACACGCATAATGGATGAAAGGAACCGACAGGTCACTTT
  TACAAAGAGAAAGTTTGGATTAATGAAGAAAGCCTATGAACTTAGTGTGCTCTGTGACTG
  TGAAATAGCACTCATCATTTTCAACAGCTCTAACAAACTGTTTCAATATGCTAGCACTGA
  TATGGACAAAGTTCTTCTCAAGTATACAGAATATAATGAACCTCATGAAAGCAGAACCAA
  CTCGGATATTGTTGAGGCTCTGAACAAGAAGGAACACAGAGGGTGCGACAGCCCAGACCC
  TGATACTTCATATGTGCTAACTCCACATACAGAAGAAAAATATAAAAAAATTAATGAGGA
  ATTTGATAATATGATGCGGAATCATAAAATCGCACCTGGTCTGCCACCTCAGAACTTTTC
  AATGTCTGTCACAGTTCCAGTGACCAGCCCCAATGCTTTGTCCTACACTAACCCAGGGAG
  TTCACTGGTGTCCCCATCTTTGGCAGCCAGCTCAACGTTAACAGATTCAAGCATGCTCTC
  TCCACCTCAAACCACATTACATAGAAATGTGTCTCCTGGAGCTCCTCAGAGACCACCAAG
  TACTGGCAATGCAGGTGGGATGTTGAGCACTACAGACCTCACAGTGCCAAATGGAGCTGG
  AAGCAGTCCAGTGGGGAATGGATTTGTAAACTCAAGAGCTTCTCCAAATTTGATTGGAGC
  TACTGGTGCAAATAGCTTAGGCAAAGTCATGCCTACAAAGTCTCCCCCTCCACCAGGTGG
  TGGTAATCTTGGAATGAACAGTAGGAAACCAGATCTTCGAGTTGTCATCCCCCCTTCAAG
  CAAGGGCATGATGCCTCCACTATCGGAGGAAGAGGAATTGGAGTTGAACACCCAAAGGAT
  CAGTAGTTCTCAAGCCACTCAACCTCTTGCTACCCCAGTCGTGTCTGTGACAACCCCAAG
  CTTGCCTCCGCAAGGACTTGTGTACTCAGCAATGCCGACTGCCTACAACACTGATTATTC
  ACTGACCAGCGCTGACCTGTCAGCCCTTCAAGGCTTCAACTCGCCAGGAATGCTGTCGCT
  GGGACAGGTGTCGGCCTGGCAGCAGCACCACCTAGGACAAGCAGCCCTCAGCTCTCTTGT
  TGCTGGAGGGCAGTTATCTCAGGGTTCCAATTTATCCATTAATACCAACCAAAACATCAG
  CATCAAGTCCGAACCGATTTCACCTCCTCGGGATCGTATGACCCCATCGGGCTTCCAGCA
  GCAGCAGCAGCAGCAGCAGCAGCAGCAGCCGCCGCCACCACCGCAGCCCCAGCCACAACC
  CCCGCAGCCCCAGCCCCGACAGGAAATGGGGCGCTCCCCTGTGGACAGTCTGAGCAGCTC
  TAGTAGCTCCTATGATGGCAGTGATCGGGAGGATCCACGGGGCGACTTCCATTCTCCAAT
  TGTGCTTGGCCGACCCCCAAACACTGAGGACAGAGAAAGCCCTTCTGTAAAGCGAATGAG
  GATGGACGCGTGGGTGACCTAAGGCTTCCAAGCTGATGTTTGTACTTTTGTGTTACTGCA
  GTGACCTGCCCTACATATCTAAATCGGTAAATAAGGACATGAGTTAAATATATTTATATG
  TACATACATATATATATCCCTTTACATATATATGTATGTGGGTGTGAGTGTGTGTGTATG
  TGTGGGTGTGTGTTACATACACAGAATCAGGCACTTACCTGCAAACTCCTTGTAGGTCTG
  CAGATGTGTGTCCCATGGCAGACAAAGCACCCTGTAGGCACAGACAAGTCTGGCACTTCC
  TTGGACTACTTGTTTCGTAAAGATAACCAGTTTTTGCAGAGAAACGTGTACCCATATATA
  ATTCTCCCACACTAGCTTGCAGAAACCTAGAGGGCCCCCTACTTGTTTTATTTAACTGTG
  CAGTGACTGTAGTTACTTAAGAGAAAATGCTTTGTAGAACAGAGCAGTAGAAAAGCAGGA
  ACCAAGAAAGCAATACTGTACATAAAATGTCATTTATATTTTCCAACCTGGCATGGGTGT
  CTGTTGCAAAGGGGTGCATGGGAAAGGGCTGTTGATATTAAAAACAAACAAAACAAAAAA
  GCCCCACACATAACTGTTTTGCACGTGCAAAAATGTATTGGGTCAAGAAGTGATCTTTAG
  CTAATAAAGAAAGAGAATAGAAAACACGCATGAGATATTCAGAAAATACTAGCCTAGAAA
  TATAGAGCATTAACAAAGKAAAATTAATATATTAAGTTATAATTGGAATATGTCAGAAGT
  TTCTTTTTACATTCATATCTTAAAAATTAAAGAAACTGATTTTAGCTCATGTATATTTTA
  TATGAAAGAAAACACCCTTATGAATTGATGACTATATATAAAATTATATTCACTACTTTT
  GAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005587 unedited
  TGCAGTTCTGATTTGTATACGACTCATATAGGCGGCCGCGTATTCCCGGGATATCGTCGA
  CCCACGCGTCCGGTCCCGGCGAGTCCCGGGCTGAAAGAGCGGCTCCGGGCGGCGCGAAGC
  GCTGGTGGCGGGCCCGGGCTGCGGCGTGTGCGCGCCCGCCAGCTGCTCCGGAGATACGGA
  ATTGCATTTTGTGAAAAAAGAACAAGAATTTTCTGCAAGGATCATATCTAAGTGCACTTT
  TTGCTGATACTTCATTTCTAATCTTGTAGAAAATTTCAGCTGTAGCCCTTGGACTAGAAG
  CTGAAATAACAGAAGCTGTGTACGATGCATTAGGGTATTGAAGAAAATTAACTTTTGAAT
  TAAATATTTGGAATATAAGGAAATAAGGAAAGTTGACTGAAAATGGGGCGGAAGAAAATA
  CAAATCACACGCATAATGGATGAAAGGAACCGACAGGTCACTTTTACAAAGAGAAAGTTT
  GGATTAATGAAGAAAGCCTATGAACTTAGTGTGCTCTGTGACTGTGAAATAGCACTCATC
  ATTTTCAACAGCTCTAACAAACTGTTTCAATATGCTAGCACTGATATGGACAAAGTTCTT
  CTCAAGTATACAGAATATAATGAACCTCATGAAAGCAGAACCAACTCGGATATTGTTGAG
  GCTCTGAACAAGAAGGAACACAGAGGGTGCGACAGCCCAGACCCTGATACTTCATATGTG
  CTAACTCCACATACNGAAGAAAAAATATAAANAATTAATGAGGAATTTGATAATATGATG
  CGGAATCATAAAATCGCACCTGGTCTGCCACCTCAGAACTTTTCAATGTCTGTCACAGTT
  CCAGTGACCAGCCCCCATGCTTTGTCCTACACTAACCCAGGAGTTCACTGGTGTCCCCAT
  CTTTGCAGCCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005587
• Insert Size: 2900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005587.1, NP_005578.1
• RefSeq Size: 2975 bp
• RefSeq ORF: 1524 bp
• Locus ID: 4205
• Cytogenetics: 15q26.3
• Domains: MADS
• Protein Families: Transcription Factors
• Gene Summary: 'The protein encoded by this gene is a DNA-binding transcription factor that activates many muscle-specific, growth factor-induced, and stress-induced genes. The encoded protein can act as a homodimer or as a heterodimer and is involved in several cellular processes, including muscle development, neuronal differentiation, cell growth control, and apoptosis. Defects in this gene could be a cause of autosomal dominant coronary artery disease 1 with myocardial infarction (ADCAD1). Several transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Jan 2010]'
  Transcript Variant: This variant (1) lacks multiple, in-frame coding exons and uses an alternate coding exon, compared to transcript variant 6. These differences result in a shorter isoform (1), compared to isoform 5. Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.