MDMX (MDM4) (NM_002393) Human Untagged Clone

CAT#: SC310334

MDM4 (untagged)-Human Mdm4 p53 binding protein homolog (mouse) (MDM4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MDM4
• Synonyms: HDMX; MDMX; MRP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002393 edited
  TGGAGCTGCCGTAAGTTTTACCAACAGACTGCAGTTTCTTCACTACCAAAATGACATCAT
  TTTCCACCTCTGCTCAGTGTTCAACATCTGACAGTGCTTGCAGGATCTCTCCTGGACAAA
  TCAATCAGGTACGACCAAAACTGCCGCTTTTGAAGATTTTGCATGCAGCAGGTGCGCAAG
  GTGAAATGTTCACTGTTAAAGAGGTCATGCACTATTTAGGTCAGTACATAATGGTGAAGC
  AACTTTATGATCAGCAGGAGCAGCATATGGTATATTGTGGTGGAGATCTTTTGGGAGAAC
  TACTGGGACGTCAGAGCTTCTCCGTGAAAGACCCAAGCCCTCTCTATGATATGCTAAGAA
  AGAATCTTGTCACTTTAGCCACTGCTACTACAGATGCTGCTCAGACTCTCGCTCTCGCAC
  AGGATCACAGTATGGATATTCCAAGTCAAGACCAACTGAAGCAAAGTGCAGAGGAAAGTT
  CCACTTCCAGAAAAAGAACTACAGAAGACGATATCCCCACACTGCCTACCTCAGAGCATA
  AATGCATACATTCTAGAGAAGATGAAGACTTAATTGAAAATTTAGCCCAAGATGAAACAT
  CTAGGCTGGACCTTGGATTTGAGGAGTGGGATGTAGCTGGCCTGCCTTGGTGGTTTTTAG
  GAAACTTGAGAAGCAACTATACACCTAGAAGTAATGGCTCAACTGATTTACAGACAAATC
  AGGATGTGGGTACTGCCATTGTTTCAGATACTACAGATGACTTGTGGTTTTTGAATGAGT
  CAGTATCAGAGCAGTTAGGTGTTGGAATAAAAGTTGAAGCTGCTGATACTGAACAAACAA
  GTGAAGAAGTAGGGAAAGTAAGTGACAAAAAGGTGATTGAAGTGGGAAAAAATGATGACC
  TGGAGGACTCTAAGTCCTTAAGTGATGATACCGATGTAGAGGTTACCTCTGAGGATGAGT
  GGCAGTGTACTGAATGCAAGAAATTTAACTCTCCAAGCAAGAGGTACTGTTTTCGTTGTT
  GGGCCTTGAGGAAGGATTGGTATTCAGATTGTTCAAAGTTAACCCATTCTCTCTCCACGT
  CTGATATCACTGCCATACCTGAAAAGGAAAATGAAGGAAATGATGTCCCTGATTGTCGAA
  GAACCATTTCGGCTCCTGTCGTTAGACCTAAAGATGCGTATATAAAGAAAGAAAACTCCA
  AACTTTTTGATCCCTGCAACTCAGTGGAATTCTTGGATTTGGCTCACAGTTCTGAAAGCC
  AAGAGACCATCTCAAGCATGGGAGAACAGTTAGATAACCTTTCTGAACAGAGAACAGATA
  CAGAAAACATGGAGGATTGCCAGAATCTCTTGAAGCCATGTAGCTTATGTGAGAAAAGAC
  CACGAGACGGGAACATTATTCATGGAAGGACGGGCCATCTTGTCACTTGTTTTCACTGTG
  CCAGAAGACTAAAGAAGGCTGGGGCTTCATGCCCTATTTGCAAGAAAGAGATTCAGCTGG
  TTATTAAGGTTTTTATAGCATAATGGTAGTACGAACATAAAAATGCATTTATTCAGTTCA
  CTTACCACATTATTTGAAAATCAATCCTTTATTTAATTTTATTTCCAACCTGTCAGAGAA
  TGTTCTTAGGCATCAAAATCCAAGGTAGCTG
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_002393 unedited
  GTCAAGGTCAGAATTGTATACGACTCATATAGGCGGCCGCCAGTGTGATGGAATCTGCAG
  AATTCGCCCTTTGGAGCTGCCGTAAGTTTTACCAACAGACTGCAGTTTCTTCACTACCAA
  AATGACATCATTTTCCACCTCTGCTCAGTGTTCAACATCTGACAGTGCTTGCAGGATCTC
  TCCTGGACAAATCAATCAGGTACGACCAAAACTGCCGCTTTTGAAGATTTTGCATGCAGC
  AGGTGCGCAAGGTGAAATGTTCACTGTTAAAGAGGTCATGCACTATTTAGGTCAGTACAT
  AATGGTGAAGCAACTTTATGATCAGCAGGAGCAGCATATGGTATATTGTGGTGGAGATCT
  TTTGGGAGAACTACTGGGACGTCAGAGCTTCTCCGTGAAAGACCCAAGCCCTCTCTATGA
  TATGCTAAGAAAGAATCTTGTCACTTTAGCCACTGCTACTACAGATGCTGCTCAGACTCT
  CGCTCTCGCACAGGATCACAGTATGGATATTCCAAGTCAAGACCAACTGAAGCAAAGTGC
  AGAGGAAAGTTCCACTTCCAGAAAAAGAACTACAGAAGACGATATCCCCACACTGCCTAC
  CTCAGAGCATAAATGCATACATTCTAGAGAAGATGAAGACTTAATTGAAAATTTAGCCCA
  AGATGAAACATCTAGGCTGGACCTTNGGATTGAGGAGTGGGATGTAGCTGGCCTGCCTTG
  GTGGTTTTTAGGAAACTTGAGAAGCACTATACACTAGGAAGTATGGCTCAACTGATTTAC
  AGACAAATCANGATGTGGGGTACTGGCCATTGTTTCAGATACTACAGATGACTTGTGGGT
  TTTTGAATGAATCAGTATCAGAAGCGTTTAGGTGTTTGGATAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' genomic read for NM_002393 unedited
  CTTTGGNNGATGGCACTTNCCAGGNCCAGNAAAGCACTGGGGNAGGGTCACAGGATGCCA
  CCCGGGATCTGTTCAGGAAACAGCTATGACCGCGGCCGCGAATTCAGATCTGGTACCGAG
  CTCGGATCCACTAGTAACGGCCGCCAGTGTGCTGGAATTCGCCCTTCAGCTACCTTGGAT
  TTTGATGCCTAAGAACATTCTCTGACAGGTTGGAAATAAAATTAAATAAAGGATTGATTT
  TCAAATAATGTGGTAAGTGAACTGAATAAATGCATTTTTATGTTCGTACTACCATTATGC
  TATAAAAACCTTAATAACCAGCTGAATCTCTTTCTTGCAAATAGGGCATGAAGCCCCAGC
  CTTCTTTAGTCTTCTGGCACAGTGAAAACAAGTGACAAGATGGCCCGTCCTTCCATGAAT
  AATGTTCCCGTCTCGTGGTCTTTTCTCACATAAGCTACATGGCTTCAAGAGATTCTGGCA
  ATCCTCCATGTTTTCTGTATCTGTTCTCTGTTCAGAAAGGTTATCTAACTGTTCTCCCAT
  GCTTGAGATGGTCTCTTGGCTTTCAGAACTGTGAGCCAAATCCAAGAATTCCACTGAGTT
  GCAGGGATCAAAAAGTTTGGAGTTTTCTTTCTTTATATACGCATCTTTAGGTCTAACGAC
  AGGAGCCGAAATGGTTCTTCGACAATCAGGGACATCATTTCCTTCATTTTCCTTTTCAGG
  TATGGCAGTGATATCAGACGTGGAGAGAGAATGGGTTAACTTTGAACAATCTGAATACCA
  ATCCTTCCTCAGGCCCCAACACGAAAACAGTACCTCTTGCTTGGAGAGTAAAATTTCTGC
  TTCAGTACACTGCCACTCATCCTAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_002393
• Insert Size: 1700 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: There are 1 nucleotide differences between the OriGene clone and the NCBI reference ORF. OriGene considers these to be polymorphisms and to reflect the natural differences between individuals. These result in the substitution of 1 amino acid.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002393.2, NP_002384.2
• RefSeq Size: 2554 bp
• RefSeq ORF: 1473 bp
• Locus ID: 4194
• Cytogenetics: 1q32.1
• Domains: SWIB
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: p53 signaling pathway
• Gene Summary: 'This gene encodes a nuclear protein that contains a p53 binding domain at the N-terminus and a RING finger domain at the C-terminus, and shows structural similarity to p53-binding protein MDM2. Both proteins bind the p53 tumor suppressor protein and inhibit its activity, and have been shown to be overexpressed in a variety of human cancers. However, unlike MDM2 which degrades p53, this protein inhibits p53 by binding its transcriptional activation domain. This protein also interacts with MDM2 protein via the RING finger domain, and inhibits the latter's degradation. So this protein can reverse MDM2-targeted degradation of p53, while maintaining suppression of p53 transactivation and apoptotic functions. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Feb 2011]'
  Transcript Variant: This variant (1, also known as MDM4-FL or HDMX) represents the predominant transcript, and encodes the longest isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data because no single transcript was available for the full length of the gene. The extent of this transcript is supported by transcript alignments.