Nucleobindin 2 (NUCB2) (NM_005013) Human Untagged Clone

CAT#: SC310412

NUCB2 (untagged)-Human nucleobindin 2 (NUCB2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NUCB2
• Synonyms: HEL-S-109; NEFA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005013 edited
  GCCACCATGAGGTGGAGGACCATCCTGCTACAGTATTGCTTTCTCTTGATTACATGTTTA
  CTTACTGCTCTTGAAGCTGTGCCTATTGACATAGACAAGACAAAAGTACAAAATATTCAC
  CCTGTGGAAAGTGCGAAGATAGAACCACCAGATACTGGACTTTATTATGATGAATATCTC
  AAGCAAGTGATTGATGTGCTGGAAACAGATAAACACTTCAGAGAAAAGCTCCAGAAAGCA
  GACATAGAGGAAATAAAGAGTGGGAGGCTAAGCAAAGAACTGGATTTAGTAAGTCACCAT
  GTGAGGACAAAACTTGATGAACTGAAAAGGCAAGAAGTAGGAAGGTTAAGAATGTTAATT
  AAAGCTAAGTTGGATTCCCTTCAAGATATAGGCATGGACCACCAAGCTCTTCTAAAACAA
  TTTGATCACCTAAACCACCTGAATCCTGACAAGTTTGAATCCACAGATTTAGATATGCTA
  ATCAAAGCGGCAACAAGTGATCTGGAACACTATGACAAGACTCGTCATGAAGAATTTAAA
  AAATATGAAATGATGAAGGAACATGAAAGGAGAGAATATTTAAAAACATTGAATGAAGAA
  AAGAGAAAAGAAGAAGAGTCTAAATTTGAAGAAATGAAGAAAAAGCATGAAAATCACCCT
  AAAGTTAATCACCCAGGAAGCAAAGATCAACTAAAAGAGGTATGGGAAGAGACTGATGGA
  TTGGATCCTAATGACTTTGACCCCAAGACATTTTTCAAATTACATGATGTCAATAGTGAT
  GGATTCCTGGATGAACAAGAATTAGAAGCCCTATTTACTAAAGAGTTGGAGAAAGTATAT
  GACCCTAAAAATGAAGAGGATGATATGGTAGAAATGGAAGAAGAAAGGCTTAGAATGAGG
  GAACATGTAATGAATGAGGTTGATACTAACAAAGACAGATTGGTGACTCTGGAGGAGTTT
  TTGAAAGCCACAGAAAAAAAAGAATTCTTGGAGCCAGATAGCTGGGAGACATTAGATCAG
  CAACAGTTCTTCACAGAGGAAGAACTAAAAGAATATGAAAATATTATTGCTTTACAAGAA
  AATGAACTTAAGAAGAAGGCAGATGAGCTTCAGAAACAAAAAGAAGAGCTACAACGTCAG
  CATGATCAACTGGAGGCTCAGAAGCTGGAATATCATCAGGTCATACAGCAGATGGAACAA
  AAAAAATTACAACAAGGAATTCCTCCATCAGGGCCAGCTGGAGAATTGAAGTTTGAGCCA
  CACATTTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_005013
• Insert Size: 1300 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005013.1, NP_005004.1
• RefSeq Size: 1586 bp
• RefSeq ORF: 1263 bp
• Locus ID: 4925
• Cytogenetics: 11p15.1
• Domains: EFh
• Protein Families: Secreted Protein, Transmembrane
• Gene Summary: 'This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]'