Nucleobindin 2 (NUCB2) (NM_005013) Human Untagged Clone

CAT#: SC310412

NUCB2 (untagged)-Human nucleobindin 2 (NUCB2)


  "NM_005013" in other vectors (6)

Reconstitution Protocol

USD 710.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol NUCB2
Synonyms HEL-S-109; NEFA
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_005013 edited
GCCACCATGAGGTGGAGGACCATCCTGCTACAGTATTGCTTTCTCTTGATTACATGTTTA
CTTACTGCTCTTGAAGCTGTGCCTATTGACATAGACAAGACAAAAGTACAAAATATTCAC
CCTGTGGAAAGTGCGAAGATAGAACCACCAGATACTGGACTTTATTATGATGAATATCTC
AAGCAAGTGATTGATGTGCTGGAAACAGATAAACACTTCAGAGAAAAGCTCCAGAAAGCA
GACATAGAGGAAATAAAGAGTGGGAGGCTAAGCAAAGAACTGGATTTAGTAAGTCACCAT
GTGAGGACAAAACTTGATGAACTGAAAAGGCAAGAAGTAGGAAGGTTAAGAATGTTAATT
AAAGCTAAGTTGGATTCCCTTCAAGATATAGGCATGGACCACCAAGCTCTTCTAAAACAA
TTTGATCACCTAAACCACCTGAATCCTGACAAGTTTGAATCCACAGATTTAGATATGCTA
ATCAAAGCGGCAACAAGTGATCTGGAACACTATGACAAGACTCGTCATGAAGAATTTAAA
AAATATGAAATGATGAAGGAACATGAAAGGAGAGAATATTTAAAAACATTGAATGAAGAA
AAGAGAAAAGAAGAAGAGTCTAAATTTGAAGAAATGAAGAAAAAGCATGAAAATCACCCT
AAAGTTAATCACCCAGGAAGCAAAGATCAACTAAAAGAGGTATGGGAAGAGACTGATGGA
TTGGATCCTAATGACTTTGACCCCAAGACATTTTTCAAATTACATGATGTCAATAGTGAT
GGATTCCTGGATGAACAAGAATTAGAAGCCCTATTTACTAAAGAGTTGGAGAAAGTATAT
GACCCTAAAAATGAAGAGGATGATATGGTAGAAATGGAAGAAGAAAGGCTTAGAATGAGG
GAACATGTAATGAATGAGGTTGATACTAACAAAGACAGATTGGTGACTCTGGAGGAGTTT
TTGAAAGCCACAGAAAAAAAAGAATTCTTGGAGCCAGATAGCTGGGAGACATTAGATCAG
CAACAGTTCTTCACAGAGGAAGAACTAAAAGAATATGAAAATATTATTGCTTTACAAGAA
AATGAACTTAAGAAGAAGGCAGATGAGCTTCAGAAACAAAAAGAAGAGCTACAACGTCAG
CATGATCAACTGGAGGCTCAGAAGCTGGAATATCATCAGGTCATACAGCAGATGGAACAA
AAAAAATTACAACAAGGAATTCCTCCATCAGGGCCAGCTGGAGAATTGAAGTTTGAGCCA
CACATTTAA
Restriction Sites Please inquire     
ACCN NM_005013
Insert Size 1300 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_005013.1, NP_005004.1
RefSeq Size 1586 bp
RefSeq ORF 1263 bp
Locus ID 4925
Cytogenetics 11p15.1
Domains EFh
Protein Families Secreted Protein, Transmembrane
Gene Summary 'This gene encodes a protein with a suggested role in calcium level maintenance, eating regulation in the hypothalamus, and release of tumor necrosis factor from vascular endothelial cells. This protein binds calcium and has EF-folding domains. [provided by RefSeq, Oct 2011]'

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