KIR5.1 (KCNJ16) (NM_018658) Human Untagged Clone

CAT#: SC310418

KCNJ16 (untagged)-Human potassium inwardly-rectifying channel, subfamily J, member 16 (KCNJ16), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KCNJ16
• Synonyms: BIR9; KIR5.1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_018658 edited
  GAGAACTCAGTGGCCTTTGTCTGTGGTGAATGTATCCGGAAAGGCAAAGAAGAGAGAGAT
  TCTCTTGTGGGGTTTCTCATCTCCATGGCGGTAGGTGCTGACAGCTTCAGAAGGGTATAA
  CAGGGCCAAGAGAAAAGGGCCCTGAGATGCTCAGCTACGGTGCTCTTACAAGATGAAAAG
  GACATGGGGTTCAGATCTGCACTTCATCATCTTTCACTAAGACATCCACATTCCACCCTG
  GGCCTTGCCCGTCTGGTGAGCCCAATCTCTGCTGGTCAGTCACGCAGTTGAGGTCAGGGA
  AGGAGTAACTCAAGATGATTTTGATGTTGCAGTTTTAAATTTCACTTTGACTTGAGCTGG
  GAAATCCTTTGGCCTATTATACCATGGATGCTAAAAATGGTTCTAACTGAAAACCCAAAC
  CAAGAAATAGCAACAAGTCTAGAATTCTTACTACTACAAAACTCACCTGGATCCCTAAGG
  GCACAGCAAAGAATGAGCTATTACGGCAGCAGCTATCATATTATCAATGCGGACGCAAAA
  TACCCAGGCTACCCGCCAGAGCACATTATAGCTGAGAAGAGAAGAGCAAGAAGACGATTA
  CTTCACAAAGATGGCAGCTGTAATGTCTACTTCAAGCACATTTTTGGAGAATGGGGAAGC
  TATGTGGTTGACATCTTCACCACTCTTGTGGACACCAAGTGGCGCCATATGTTTGTGATA
  TTTTCTTTATCTTATATTCTCTCGTGGTTGATATTTGGCTCTGTCTTTTGGCTCATAGCC
  TTTCATCATGGCGATCTATTAAATGATCCAGACATCACACCTTGTGTTGACAACGTCCAT
  TCTTTCACAGGGGCCTTTTTGTTCTCCCTAGAGACCCAAACCACCATAGGATATGGTTAT
  CGCTGTGTTACTGAAGAATGTTCTGTGGCCGTGCTCATGGTGATCCTCCAGTCCATCTTA
  AGTTGCATCATAAATACCTTTATCATTGGAGCTGCCTTGGCCAAAATGGCAACTGCTCGA
  AAGAGAGCCCAAACCATTCGTTTCAGCTACTTTGCACTTATAGGTATGAGAGATGGGAAG
  CTTTGCCTCATGTGGCGCATTGGTGATTTTCGGCCAAACCACGTGGTAGAAGGAACAGTT
  AGAGCCCAACTTCTCCGCTATACAGAAGACAGTGAAGGGAGGATGACGATGGCATTTAAA
  GACCTCAAATTAGTCAACGACCAAATCATCCTGGTCACCCCGATAACTATTGTCCATGAA
  ATTGACCATGAGAGCCCTCTGTATGCCCTTGACCGCAAAGCAGTAGCCAAAGATAACTTT
  GAGATTTTGGTGACATTTATCTATACTGGTGATTCCACTGGAACATCTCACCAATCTAGA
  AGCTCCTATGTTCCCCGAGAAATTCTCTGGGGCCATAGGTTTAATGATGTCTTGGAAGTT
  AAGAGGAAGTATTACAAAGTGAACTGCTTACAGTTTGAAGGAAGTGTGGAAGTATATGCC
  CCCTTTTGCAGTGCCAAGCAATTGGACTGGAAAGACCAGCAGCTCCACATAGAAAAAGCA
  CCACCAGTTCGAGAATCCTGCACGTCGGACACCAAGGCGAGACGAAGGTCATTTAGTGCA
  GTTGCCATTGTCAGCAGCTGTGAAAACCCTGAGGAGACCACCACTTCCGCCACACATGAA
  TATAGGGAAACACCTTATCAGAAAGCTCTCCTGACTTTAAACAGAATCTCTGTAGAATCC
  CAAATGTAGTCCTAAATTGCAATTATGAGGGCTACCACTGAATCATTTTATCTTTCAGCC
  AATCAAGTCGTTGTAAACGTGGCTTTTTTGAAAGTGTTATGGCTATGTTTTATGATGATG
  CTGGGTAAGTAGAGTAAGTTAAACTTGGTAAAAGATAATCTAAAAATTCCATAGTTCTCA
  GTTATTAAAATTTTTCTTGTTCGCCAATTTTGTATTAAGAATACTATTAAGCCTAATTGA
  TTAAAATTTATCTTTTTTATTATCTTACATGCTTGTATCTTCAGTTGGAGGTGTAGTATT
  CAAAAACGGGGAATGAAGGCAGGAAGGAGGCTGGAATAAATAAAAATAAAAATAGACAAG
  TAAGACAGCATAAATAATACATTTTTAAACATGTCAACATTGATAATACAATGAAGATTT
  ACCATAAAAAGTATCATATCTAACCAAGATATGCAAAAGATGCATTCAGTAAGCTGTAAC
  GTTGAGAAATGTAACTGTGAAATCAGCTCACCAGTTCAAGTCACTAGGTTTGCCACTTGG
  ACCGTCTTGGTTTCAGCGAGCATAGCGAGTGGCTGTAAGAAATTGTCCATGCCACCAATT
  CCACTGCTACTTGCCCAGGTAGTGATCAGTGAGAGTTAGAAGCAATTTTTTCTCTAACCG
  ATGAGGCAGAAGAAAAGTTAACAGTATTTAAAGCAGCATTTCTCTCTTAACGTTCCTGGG
  AGACTCCAACAAATGGAGACCAGAAACAAGGCAACCACGACCTTGCTAGGTTCTCTGAGG
  AGGGGACTTTCTATGTTAACGTCCCACCTTCTTCGAGGACATGACCAAGGGACAGGATCA
  TACAGATGAGGTGAGGGGATGGTTAGCAGCGATAAACATTCTCAGACCCTTGGAGAGAAA
  CCGTTGCTAGCTTGCTGGTCCCGAAAGATTGCAACTTTGGCTGCTACAGAAAGGATAGAA
  CCTTTTTGGGGGGAGGGTGGGGAGGGAGATATTTATTTGTGCCCAGAAGCAGTTATGTAT
  GCTTCTTGTCTTGGTTACTGTACAAGATACAAATGACTCGAGTTGTGGATTGCAAGAAAC
  TCTTTTATTAAACTTGGAACAACCATTTAAATGAAGGATTTTGTGAGATAGAATTCAATT
  AAAGCGATTTGAATGCACAGTAAGTGGATAATCTGAGTACAATGAAATTTCTTAAGTTTT
  AGAACATGAGATAAAGTTGTATACTCTACTTTCATGTTAAAATGTTAATCATGATTACTT
  TAAGCCCACATCCACTGAGTAAAGAGTCACTTTAAACTTTATAAATCAAAGAGATATTAG
  GAACATTCAGAATTATTTGTGTTTTGTTTTTGTTTTACATTGTTGCTGCACTGGCAATAA
  GTATGTGGCACTGTTTTTATTTGTTTATGTTATTTAAATACACACCCAGACACACACACA
  CACAGTTTTTGCTTTTTCTGGAAAATCTTTCTTTCTCTTCTCTCTCCATGAAAATTAAAA
  CACACAAGTGAATGAAGTAATTACATAGTCCAAGATTAGAAATACAAGTAAAATATATAG
  GTATAGGTAGATAGAGTGCCTTTCTGTTTCTTTTCTTTTCTTTTTTCTTTTTTTGTTCTT
  CCTTCCTTTCTTCCCTCCTCCCCCTCATCCTTCCCTTTCTCCTTCTTTTCCCTTTTTTTT
  TTTTTTTTGACCTGGGAGTAATAAAGTTACTTTTTAAATGCACGTATGCAATCTTTTATT
  CCTCTTGTTTTATGGATAAGAGATTTGTTCAGGTCAAAAGTTGCAAAACTGTTCTGAAGC
  TTAATATTAGAGACCCAAGTAAGATTGTTTTTAGTCTTGCTTGAGGAATGCACACTCCAT
  TTAAAAAATTGTGCGCTCTGTAGATTTGGATTTTACAGCAGCAATTACTTGGCTAAATTA
  GAGTACTGCAATCTTGTAAGTAGAAAACAGCCACCATGACAATAAACAAAAACACTTAAA
  AATATTTTTAGCTTGTTTTACCTACATCTAGTTTCATTTCATCTTGCTTTGCTTTGTTGA
  CTCTGCTACCGCTGATGTTTGCCTTGTCAATATCAGAATAGGGGCATCAGTGTCCCGTGA
  AATACATTTTGTTGTGCTATGAGCCTCTGTAATCAAATGCTTGTACTCATAAGAGAGTGT
  AAAATCAGCATTTCTATGTAACATATATCTCTAATTATCTGTGTAATTTTATAACATACT
  TTGACAACCCCAGTGAGTTACTTAATAATAAAGATCTTAAAATAATGCAAAAAAAAAAAA
  AAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_018658
• Insert Size: 4000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to contain one SNP compared with NM_018658.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018658.1, NP_061128.1
• RefSeq Size: 4021 bp
• RefSeq ORF: 1257 bp
• Locus ID: 3773
• Cytogenetics: 17q24.3
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• Gene Summary: 'Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which tends to allow potassium to flow into rather than out of a cell, can form heterodimers with two other inward-rectifier type potassium channels. It may function in fluid and pH balance regulation. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Apr 2014]'
  Transcript Variant: This variant (1) encodes the longer isoform (a). Variants 1, 2, 3, 5 and 6 all encode isoform a.