PPM1B (NM_177968) Human Untagged Clone

CAT#: SC310457

PPM1B (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1B (PPM1B), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPM1B
• Synonyms: PP2C-beta; PP2C-beta-X; PP2CB; PP2CBETA; PPC2BETAX
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_177968, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGTGCATTTTTGGATAAACCCAAAACTGAAAAACATAATGCTCATGGTGCTGGGAATGGTTTACGTT
  ATGGCCTGAGCAGCATGCAAGGATGGAGAGTGGAAATGGAAGATGCACACACAGCTGTTGTAGGTATTCC
  TCACGGCTTGGAAGACTGGTCATTTTTTGCAGTTTATGATGGTCATGCTGGATCCCGAGTGGCAAATTAC
  TGCTCAACACATTTATTAGAACACATCACTACTAACGAAGACTTTAGGGCAGCTGGAAAATCAGGATCTG
  CTCTTGAGCTTTCAGTGGAAAATGTTAAGAATGGTATCAGAACTGGATTTTTGAAAATTGATGAATACAT
  GCGTAACTTTTCAGACCTCAGAAACGGGATGGACAGGAGTGGTTCAACTGCAGTGGGAGTTATGATTTCA
  CCTAAGCATATCTACTTTATCAACTGTGGTGATTCACGTGCTGTTCTGTATAGGAATGGACAAGTCTGCT
  TTTCTACCCAGGATCACAAACCTTGCAATCCAAGGGAAAAGGAGCGAATCCAAAATGCAGGAGGCAGCGT
  GATGATACAACGTGTTAATGGTTCATTAGCAGTATCTCGTGCTCTGGGGGACTATGATTACAAGTGTGTT
  GATGGCAAGGGCCCAACAGAACAACTTGTTTCTCCAGAGCCTGAGGTTTATGAAATTTTAAGAGCAGAAG
  AGGATGAATTTATCATCTTGGCTTGTGATGGGATCTGGGATGTTATGAGTAATGAGGAGCTCTGTGAATA
  TGTTAAATCTAGGCTTGAGGTATCTGATGACCTGGAAAATGTGTGCAATTGGGTAGTGGACACTTGTTTA
  CACAAGGGAAGTCGAGATAACATGAGTATTGTACTAGTTTGCTTTTCAAATGCTCCCAAGGTCTCAGATG
  AAGCGGTGAAAAAAGATTCAGAGTTGGATAAGCACTTGGAATCACGGGTTGAAGAGATTATGGAGAAGTC
  TGGCGAGGAAGGAATGCCTGATCTTGCCCATGTCATGCGCATCTTGTCTGCAGAAAATATCCCAAATTTG
  CCTCCTGGGGGAGGTCTTGCTGGCAAGCGTAATGTTATTGAAGCTGTTTATAGTAGACTGAATCCACATA
  GAGAAAGTGATGGGGGTGCTGGAGATCTAGAAGACCCATGGTAG
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_177968
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_177968.3, NP_808907.1
• RefSeq Size: 3908 bp
• RefSeq ORF: 1164 bp
• Locus ID: 5495
• Cytogenetics: 2p21
• Protein Families: Druggable Genome, Phosphatase, Stem cell - Pluripotency
• Protein Pathways: MAPK signaling pathway
• Gene Summary: 'The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. This phosphatase has been shown to dephosphorylate cyclin-dependent kinases (CDKs), and thus may be involved in cell cycle control. Overexpression of this phosphatase is reported to cause cell-growth arrest or cell death. Alternative splicing results in multiple transcript variants encoding different isoforms. Additional transcript variants have been described, but currently do not represent full-length sequences. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) contains an alternate 3' terminal exon, and it thus differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (2) has a distinct C-terminus and is shorter than isoform 1.