PSME3 (NM_176863) Human Untagged Clone

CAT#: SC310584

PSME3 (untagged)-Human proteasome (prosome, macropain) activator subunit 3 (PA28 gamma, Ki) (PSME3), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PSME3
• Synonyms: HEL-S-283; Ki; PA28-gamma; PA28G; PA28gamma; REG-GAMMA
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_176863 edited
  ATGGCCTCGTTGCTGAAGGTGGATCAGGAAGTGAAGCTCAAGGTTGATTCTTTCAGGGAG
  CGGATCACAAGTGAGGCAGAAGACTTGGTGGCAAATTTTTTCCCAAAGAAGTTATTAGAA
  CTTGATAGTTTTCTGAAGGAACCAATCTTAAACATCCATGACCTAACTCAGATCCACTCT
  GACATGAATCTCCCAGTCCCTGACCCCATTCTTCTCACCAATAGCCATGATGGACTGGAT
  GGTCCCACTTATAAGAAGCGAAGGTTGGATGAGTGTGAAGAAGCCTTCCAAGGAACCAAG
  GTGTTTGTGATGCCCAATGGGATGCTGAAAAGCAACCAGCAGCTGGTGGACATTATTGAG
  AAAGTGAAACCTGAGATCCGGCTGTTGATTGAGAAATGTAACACGCCTTCAGGCAAAGGT
  CCTCATATATGTTTTGACCTCCAGGTCAAAATGTGGGTACAGCTCCTGATTCCCAGGATA
  GAAGATGGAAACAACTTTGGGGTGTCCATTCAGGAGGAAACAGTTGCAGAGCTAAGAACT
  GTTGAGAGTGAAGCTGCATCTTATCTGGACCAGATTTCTAGATATTATATTACAAGAGCC
  AAATTGGTTTCTAAAATAGCTAAATATCCCCATGTGGAGGACTATCGCCGCACCGTGACA
  GAGATTGATGAGAAAGAATATATCAGCCTTCGGCTCATCATATCAGAGCTGAGGAATCAA
  TATGTCACTCTACATGACATGATCCTGAAAAATATCGAGAAGATCAAACGGCCCCGGAGC
  AGCAATGCAGAGACTCTGTACTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_176863 unedited
  TGCGTTTGTATACGACTCCTATAGGGCGGCCGCGTAATTCGGCACCAGGAGCGAGCGAGC
  AGTGAGTAAGCCAGCAAGGGCGGTCGGGTCCCGAGGTCAGCCGAGATTTCTCAGGTCCCT
  CCGGCCCCCTCCCTGGAGTCCACAGCGCCTCCGGTGTCCAGAGGATCGGACACGGCCCGG
  CCCGGCCATGGCCTCGTTGCTGAAGGTGGATCAGGAAGTGAAGCTCAAGGTTGATTCTTT
  CAGGGAGCGGATCACAAGTGAGGCAGAAGACTTGGTGGCAAATTTTTTCCCAAAGAAGTT
  ATTAGAACTTGATAGTTTTCTGAAGGAACCAATCTTAAACATCCATGACCTAACTCAGAT
  CCACTCTGACATGAATCTCCCAGTCCCTGACCCCATTCTTCTCACCAATAGCCATGATGG
  ACTGGATGGTCCCACTTATAAGAAGCGAAGGTTGGATGAGTGTGAAGAAGCCTTCCAAGG
  AACCAAGGTGTTTGTGATGCCCAATGGGATGCTGAAAAGCAACCAGCAGCTGGTGGACAT
  TATTGAGAAAGTGAAACCTGAGATCCGGCTGTTGATTGAGAAATGTAACACGCCTTCAGG
  CAAAGGTCCTCATATATGTTTTGACCTCCAGGTCAAAATGTGGGTACAGCTCCTGATTCC
  CAGGATAGAAGATGGAAACAACTTTGGGGTGTCCATTCAGGAGGAAACAGTTGCAGAGCT
  AAGAACTGTTGAGAGTGAAGCTGCATCTTATCTGGACCAGATTTCTAGATATTATATTAC
  AAGAGCCAAATTGGTTTCTAAATAGCTAAATATCCCCATGTGGAGGACTATCGCCGCACC
  GTGACAGAGATTGATGAGAAAGAATATATCAGCCCTTCGCTCATCATATCAGAGCTGAGA
  TCAATATGTCACCTCTACATGAACATGATCCTGAAAAATTATCGGAGAGGATC
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_176863 unedited
  TGTTCGAAGAGAACAGGAAAATCCCAGGATAGAAGATGGAAACAACTTTGGGGTGTCCAT
  TCAGGAGGAAACAGTTGCAGAGCTAAGAACTGTTGAGAGTGAAGCTGCATCTTATCTGGA
  CCAGATTTCTAGATATTATATTACAAGAGCCAAATTGGTTTCTAAAATAGCTAAATATCC
  CCATGTGGAGGACTATCGCCGCACCGTGACAGAGATTGATGAGAAAGAATATATCAGCCT
  TCGGCTCATCATATCAGAGCTGAGGAATCAATATGTCACTCTACATGACATGATCCTGAA
  AAATATCGAGAAGATCAAACGGCCCCGGAGCAGCAATGCAGAGACTCTGTACTGAGGCCA
  GGGCCAGGGCCAGGGGACTCTGTGAGTCTGGCTCAAGACCGACATTGCCTTGGTTTGTTA
  CATGACTATCGTGATGGGGAAACTGGCTGGAAATAGTAATCACACCTCTCTGTTTTTAGT
  TAGAGTCTAATGAAACTCTCATCTAGTTCTGTGATGTGTTTACCTCTTTTTTCAGGCCTC
  AGGAACTCTTCTATTTCCTTCCCTAATACCCCACACCCAACCTGTCGTAATTTCTGGAGA
  ACTCCAGGTTTGTGTGTGCAGGATGTTGGCACAAAAATACCTGTGTTTTCATTCTCCCCC
  TCTCTCCCTCCTGTGTCTTGCGCTTTATGTTTTCTTCCGTTTGATAATTAGTTGGTTAAA
  AGCTGAGGGAACCCGGAAGGAAAGTGCTAGGTGTTTTTTAGGAACTAGGGTGGCGGGGGG
  ACGAACTTCTCTTCCTCACATGAGGTTACTGTTTCTTTCCTCTGGTGGGGCATTGGATCC
  TCCCCACAGTTGCCCTGGTGATGACTTAGGCTTTCCAATCTGTGTACATCCCACTTTGAA
  TCTTGATCGGTGGACAAGAAAATAACCTTAGGCCTTTCAGTC
  
• Restriction Sites: Please inquire
• ACCN: NM_176863
• ORF Size: 804 bp
• Insert Size: 2500
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.

Reference Data

• RefSeq: NM_176863.1, NP_789839.1
• RefSeq Size: 3228
• RefSeq ORF: 804
• Locus ID: 10197
• Protein Families: Stem cell - Pluripotency
• Protein Pathways: Antigen processing and presentation, Proteasome
• Gene Summary: The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. The immunoproteasome contains an alternate regulator, referred to as the 11S regulator or PA28, that replaces the 19S regulator. Three subunits (alpha, beta and gamma) of the 11S regulator have been identified. This gene encodes the gamma subunit of the 11S regulator. Six gamma subunits combine to form a homohexameric ring. Alternate splicing results in multiple transcript variants. [provided by RefSeq, May 2012]
  Transcript Variant: This variant (2), uses ans alternate in-frame splice site, compared to variant 1. The resulting isoform (2) is longer than isoform 1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.