DPH2 (NM_001039589) Human Untagged Clone

CAT#: SC310786

DPH2 (untagged)-Human DPH2 homolog (S. cerevisiae) (DPH2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: DPH2
• Synonyms: DPH2L2
• Vector: PCMV6-Neo
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001039589 edited
  AGAAACAGTAGTTAGGATGGCTGAAGGGGATACTCACCGGCTGAAGGCCGACTGTGATTC
  CCCCTACCCCCACAAGGCGATTTTGACCCCCTGAGGGCTGCTCTAGAGGACTCAGGCCCC
  GAAGCTGTCCCAGGGAGGTCCCCGCTGCATCCCACCACCCAAGCTGTGCCTCATGGAGTC
  GATGTTTAGCAGCCCTGCCGAGGCGGCGCTGCAGCGAGAGACCGGGGTGCCAGGACTGCT
  TACTCCTCTTCCGGACCTGGACGGAGTGTACGAGCTGGAGCGAGTCGCTGGATTTGTCCG
  CGACCTGGGGTGTGAACGAGTTGCCTTGCAGTTCCCTGACCAGCTATTGGGAGATGCTGT
  GGCTGTGGCTGCACGACTGGAGGAGACGACAGGGTCAAAGATGTTCATTCTGGGTGACAC
  AGCCTACGGCAGCTGCTGCGTGGATGTGCTGGGTGCTGAGCAAGCTGGAGCTCAGGCTCT
  CATACATTTTGGCCCTGCCTGCTTAAGCCCTCCAGCCCGCCCACTGCCCGTTGCCTTCGT
  GCTTCGTCAACGTTCTGTGGCCTTGGAGCTCTGTGTCAAGGCCTTTGAGGCCCAGAACCC
  AGACCCCAAAGCGCCTGTGGTGCTGCTGAGTGAGCCGGCCTGTGCCCATGCCCTGGGCTC
  TCCCTTCCACGTGGCTCTCCCACCACCTGAGTCAGAGCTGTGGGAAACCCCAGACGTGTC
  ACTCATTACTGGAGATCTCCGACCCCCACCTGCCTGGAAGTCATCAAATGATCATGGAAG
  CTTGGCTCTGACCCCACGGCCCCAGCTGGAGCTGGCTGAGAGCAGTCCTGCAGCCTCATT
  CCTTAGTTCCCGGAGCTGGCAAGGGCTGGAGCCCCGCCTGGGTCAGACGCCAGTGACAGA
  AGCTGTGAGTGGAAGACGAGGGATTGCCATCGCCTATGAGGATGAGGGAAGCGGCTGATA
  CCATGTGGGGCTGGAGACATAGATGGACTTATGAATGGCTGCTAGGACCTTTAGTGCTCC
  CTGCACCAACCTCCCATCCCCCTGCCAAGATCCTTGAAGGACCCTGGAAGGAGGGAGAGC
  AGGCAGCCCTTCACAGGATAGGATCCGTCTCTGTCCTGTCCTGGCACTGGCACAAGCTCA
  GCACATGCCCAGTAATGCGTGTTGTTTGGCTGATGGAATAAAGGGCTTAGGGACTTCCCT
  GAGGCCTCTGGACCCATCTGTCTTCCTGAGGGCAGCCCAGGACCTTTGGCCAATCCCAGT
  TCCCAGGCTGCAGTTGAGGGTCTGTCCTTGTCAAAAGGCAGGTGCTAGACAGTCTAGACC
  AGGGTTTCTCAAACTCGTACTTGACATTTGGGGCCAGATAATTCTTTGTTGTGGGGCTGT
  CTGGTGTATGGTAGGGTGCTCAGCAGCATCCCTGGCCTCTGCCCACTAGACATCAGAAGC
  ACTCCCCCAGTTGTGACAACCAAAAATATCTCCAGACCTTGGCAAATGTTATCTGTGGGG
  GAAAATTGCCCTCAATTGAGAACCACTGGTCTAGCTAGACCTGCACTGTCCAGTACAGTA
  GCCACTAAATACATGTGGCTAAACTTAAATTTAAGTTAATTAAGATTAAAAGCTCAGTTT
  CTCAGTCACATTAGTCATTCAAGTGTTCAGACAGCCACATGAGGGGACAGTGCAGCTACA
  GGATATGCCATCATGGCAGAAAGTTCTATTGGTTGGACAGTGTTGGTCTATACTGACTCT
  TATTTCTCAGGGAGATCACAGCAACCTAAATAAACCAGATACCTTTTCTCTTAAAAAAAA
  AAAAAAAAAAAAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001039589
• Insert Size: 1800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001039589.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001039589.1, NP_001034678.1
• RefSeq Size: 1829 bp
• RefSeq ORF: 786 bp
• Locus ID: 1802
• Cytogenetics: 1p34.1
• Gene Summary: 'This gene is one of two human genes similar to the yeast gene dph2. The yeast gene was identified by its ability to complement a diphthamide mutant strain, and thus probably functions in diphthamide biosynthesis. Diphthamide is a post-translationally modified histidine residue present in elongation factor 2 (EF2) that is the target of diphtheria toxin ADP-ribosylation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2016]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.