PCB (PC) (NM_001040716) Human Untagged Clone

CAT#: SC311133

PC (untagged)-Human pyruvate carboxylase (PC), nuclear gene encoding mitochondrial protein, transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PC
• Synonyms: PCB
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001040716, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGCTGAAGTTCCGAACAGTCCATGGGGGCCTGAGGCTCCTGGGAATCCGCCGAACCTCCACCGCCCCCG
  CTGCCTCCCCAAATGTCCGGCGCCTGGAGTATAAGCCCATCAAGAAAGTCATGGTGGCCAACAGAGGTGA
  GATTGCCATCCGTGTGTTCCGGGCCTGCACGGAGCTGGGCATCCGCACCGTAGCCATCTACTCTGAGCAG
  GACACGGGCCAGATGCACCGGCAGAAAGCAGATGAAGCCTATCTCATCGGCCGCGGCCTGGCCCCCGTGC
  AGGCCTACCTGCACATCCCAGACATCATCAAGGTGGCCAAGGAGAACAACGTAGATGCAGTGCACCCTGG
  CTACGGGTTCCTCTCTGAGCGAGCGGACTTCGCCCAGGCCTGCCAGGATGCAGGGGTCCGGTTTATTGGG
  CCAAGCCCAGAAGTGGTCCGCAAGATGGGAGACAAGGTGGAGGCCCGGGCCATCGCCATTGCTGCGGGTG
  TTCCCGTTGTCCCTGGCACAGATGCCCCCATCACGTCCCTGCATGAGGCCCACGAGTTCTCCAACACCTA
  CGGCTTCCCCATCATCTTCAAGGCGGCCTATGGGGGTGGAGGGCGTGGCATGAGGGTGGTGCACAGCTAC
  GAGGAGCTGGAGGAGAATTACACCCGGGCCTACTCAGAGGCTCTGGCCGCCTTTGGGAATGGGGCGCTGT
  TTGTGGAGAAGTTCATCGAGAAGCCACGGCACATCGAGGTGCAGATCTTGGGGGACCAGTATGGGAACAT
  CCTGCACCTGTACGAGCGAGACTGCTCCATCCAGCGGCGGCACCAGAAGGTGGTCGAGATTGCCCCCGCC
  GCCCACCTGGACCCGCAGCTTCGGACTCGGCTCACCAGCGACTCTGTGAAACTCGCTAAACAGGTGGGCT
  ACGAGAACGCAGGCACCGTGGAGTTCCTGGTGGACAGGCACGGCAAGCACTACTTCATCGAGGTCAACTC
  CCGCCTGCAGGTGGAGCACACGGTCACAGAGGAGATCACCGACGTAGACCTGGTCCATGCTCAGATCCAC
  GTGGCTGAGGGCAGGAGCCTACCCGACCTGGGCCTGCGGCAGGAGAACATCCGCATCAACGGGTGTGCCA
  TCCAGTGCCGGGTCACCACCGAGGACCCCGCGCGCAGCTTCCAGCCGGACACCGGCCGCATTGAGGTGTT
  CCGGAGCGGAGAGGGCATGGGCATCCGCCTGGATAATGCTTCCGCCTTCCAAGGAGCCGTCATCTCGCCC
  CACTACGACTCCCTGCTGGTCAAAGTCATTGCCCACGGCAAAGACCACCCCACGGCCGCCACCAAGATGA
  GCAGGGCCCTTGCGGAGTTCCGCGTCCGAGGTGTGAAGACCAACATCGCCTTCCTGCAGAATGTGCTCAA
  CAACCAGCAGTTCCTGGCAGGCACTGTGGACACCCAGTTCATCGACGAGAACCCAGAGCTGTTCCAGCTG
  CGGCCTGCACAGAACCGGGCCCAAAAGCTGTTGCACTACCTCGGCCATGTCATGGTAAACGGTCCAACCA
  CCCCGATTCCCGTCAAGGCCAGCCCCAGCCCCACGGACCCCGTTGTCCCTGCAGTGCCCATAGGCCCGCC
  CCCGGCTGGTTTCAGAGACATCCTGCTGCGAGAGGGGCCTGAGGGCTTTGCTCGAGCTGTGCGGAACCAC
  CCGGGGCTGCTGCTGATGGACACGACCTTCAGGGACGCCCACCAGTCACTGCTGGCCACTCGTGTGCGCA
  CCCACGATCTCAAAAAGATCGCCCCCTATGTTGCCCACAACTTCAGCAAGCTCTTCAGCATGGAGAACTG
  GGGAGGAGCCACGTTTGACGTCGCCATGCGCTTCCTGTATGAGTGCCCCTGGCGGCGGCTGCAGGAGCTC
  CGGGAGCTCATCCCCAACATCCCTTTCCAGATGCTGCTGCGGGGGGCCAATGCTGTGGGCTACACCAACT
  ACCCAGACAACGTGGTCTTCAAGTTCTGTGAAGTGGCCAAAGAGAATGGCATGGATGTCTTCCGTGTGTT
  TGACTCCCTCAACTACTTGCCCAACATGCTGCTGGGCATGGAGGCGGCAGGAAGTGCCGGAGGCGTGGTG
  GAGGCTGCCATCTCATACACGGGCGACGTGGCCGACCCCAGCCGCACCAAGTACTCACTGCAGTACTACA
  TGGGCTTGGCCGAAGAGCTGGTGCGAGCTGGCACCCACATCCTGTGCATCAAGGACATGGCCGGGCTGCT
  GAAGCCCACGGCCTGCACCATGCTGGTCAGCTCCCTCCGGGACCGCTTCCCCGACCTCCCACTGCACATC
  CACACCCACGACACGTCAGGGGCAGGCGTGGCAGCCATGCTGGCCTGTGCCCAGGCTGGAGCTGATGTGG
  TGGATGTGGCAGCTGATTCCATGTCTGGGATGACTTCACAGCCCAGCATGGGGGCCCTGGTGGCCTGTAC
  CAGAGGGACTCCCCTGGACACAGAGGTGCCCATGGAGCGCGTGTTTGACTACAGTGAGTACTGGGAGGGG
  GCTCGGGGACTGTACGCGGCCTTCGACTGCACGGCCACCATGAAGTCTGGCAACTCGGACGTGTATGAAA
  ATGAGATCCCAGGGGGCCAGTACACCAACCTGCACTTCCAGGCCCACAGCATGGGGCTTGGCTCCAAGTT
  CAAGGAGGTCAAGAAGGCCTATGTGGAGGCCAACCAGATGCTGGGCGATCTCATCAAGGTGACGCCCTCC
  TCCAAGATCGTGGGGGACCTGGCCCAGTTTATGGTGCAGAATGGATTGAGCCGGGCAGAGGCCGAAGCTC
  AGGCGGAAGAGCTGTCCTTTCCCCGCTCCGTGGTGGAGTTCCTGCAGGGCTACATCGGTGTCCCCCATGG
  GGGGTTCCCCGAACCCTTTCGCTCTAAGGTACTGAAGGACCTGCCAAGGGTGGAGGGGCGGCCTGGAGCC
  TCCCTCCCTCCCCTGGATCTGCAGGCACTGGAGAAGGAGCTGGTAGACCGGCATGGGGAGGAGGTGACGC
  CGGAAGATGTGCTCTCAGCAGCTATGTACCCCGATGTGTTTGCCCACTTCAAGGACTTCACTGCCACCTT
  TGGCCCCCTGGATAGCCTGAATACTCGCCTCTTCCTGCAGGGACCCAAGATCGCAGAGGAGTTTGAGGTG
  GAGCTGGAGCGGGGCAAGACGCTGCACATCAAAGCCCTGGCCGTGAGCGACCTGAACCGGGCCGGCCAGA
  GGCAGGTCTTCTTTGAGCTCAATGGGCAGCTGCGGTCCATCTTGGTCAAGGACACCCAGGCCATGAAGGA
  GATGCACTTCCACCCCAAGGCCCTAAAGGACGTGAAGGGCCAGATCGGGGCGCCCATGCCTGGGAAGGTG
  ATAGACATCAAAGTGGTGGCAGGGGCCAAGGTGGCCAAGGGCCAGCCCCTGTGTGTGCTCAGTGCCATGA
  AGATGGAGACTGTGGTGACCTCACCCATGGAGGGTACTGTCCGCAAGGTTCATGTGACCAAGGACATGAC
  ACTGGAAGGTGACGACCTCATCCTGGAGATCGAGTGA
  
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001040716
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040716.1, NP_001035806.1
• RefSeq Size: 4192 bp
• RefSeq ORF: 3537 bp
• Locus ID: 5091
• Cytogenetics: 11q13.2
• Protein Families: Druggable Genome
• Protein Pathways: Citrate cycle (TCA cycle), Metabolic pathways, Pyruvate metabolism
• Gene Summary: 'This gene encodes pyruvate carboxylase, which requires biotin and ATP to catalyse the carboxylation of pyruvate to oxaloacetate. The active enzyme is a homotetramer arranged in a tetrahedron which is located exclusively in the mitochondrial matrix. Pyruvate carboxylase is involved in gluconeogenesis, lipogenesis, insulin secretion and synthesis of the neurotransmitter glutamate. Mutations in this gene have been associated with pyruvate carboxylase deficiency. Alternatively spliced transcript variants with different 5' UTRs, but encoding the same protein, have been found for this gene. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (3) also results from the use of a more distal promoter, however, it contains an additional 5' non-coding exon, hence has a longer 5' UTR compared to transcript variant 1. Transcript variants 1-3 encode the same protein.