JAK3 (NM_000215) Human Untagged Clone

CAT#: SC311134

JAK3 (untagged)-Human Janus kinase 3 (JAK3)


  "NM_000215" in other vectors (6)

Reconstitution Protocol

SC311134 is the updated version of SC125449.

USD 2,290.00

In Stock*

Size
    • 10 ug

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Specifications

Product Data
Type Human Untagged Clone
Tag Tag Free
Symbol JAK3
Synonyms JAK-3; JAK3_HUMAN; JAKL; L-JAK; LJAK
Vector pCMV6-XL5
E. coli Selection Ampicillin (100 ug/mL)
Mammalian Cell Selection None
Sequence Data
>OriGene sequence for NM_000215 edited
CCGGGATCCCGGCTCTGCCCGCCCTTCGAAAGTCCAGGGTCCCCGCCCGCTAGGACTGAG
GGGCTTTTTCTCTCTGTGCCCCAGGCAAGTTGCACTCATGGCACCTCCAAGTGAAGAGAC
GCCCCTGATCCCTCAGCGTTCATGCAGCCTCTTGTCCACGGAGGCTGGTGCCCTGCATGT
GCTGCTGCCCGCTCGGGGCCCCGGGCCCCCCCAGCGCCTATCTTTCTCCTTTGGGGACCA
CTTGGCTGAGGACCTGTGCGTGCAGGCTGCCAAGGCCAGCGGCATCCTGCCTGTGTACCA
CTCCCTCTTTGCTCTGGCCACGGAGGACCTGTCCTGCTGGTTCCCCCCGAGCCACATCTT
CTCCGTGGAGGATGCCAGCACCCAAGTCCTGCTGTACAGGATTCGCTTTTACTTCCCCAA
TTGGTTTGGGCTGGAGAAGTGCCACCGCTTCGGGCTACGCAAGGATTTGGCCAGTGCTAT
CCTTGACCTGCCAGTCCTGGAGCACCTCTTTGCCCAGCACCGCAGTGACCTGGTGAGTGG
GCGCCTCCCCGTGGGCCTCAGTCTCAAGGAGCAGGGTGAGTGTCTCAGCCTGGCCGTGTT
GGACCTGGCCCGGATGGCGCGAGAGCAGGCCCAGCGGCCGGGAGAGCTGCTGAAGACTGT
CAGCTACAAGGCCTGCCTACCCCCAAGCCTGCGCGACCTGATCCAGGGCCTGAGCTTCGT
GACGCGGAGGCGTATTCGGAGGACGGTGCGCAGAGCCCTGCGCCGCGTGGCCGCCTGCCA
GGCAGACCGGCACTCGCTCATGGCCAAGTACATCATGGACCTGGAGCGGCTGGATCCAGC
CGGGGCCGCCGAGACCTTCCACGTGGGCCTCCCTGGGGCCCTTGGTGGCCACGACGGGCT
GGGGCTGCTCCGCGTGGCTGGTGACGGCGGCATCGCCTGGACCCAGGGAGAACAGGAGGT
CCTCCAGCCCTTCTGCGACTTTCCAGAAATCGTAGACATTAGCATCAAGCAGGCCCCGCG
CGTTGGCCCGGCCGGAGAGCACCGCCTGGTCACTGTTACCAGGACAGACAACCAGATTTT
AGAGGCCGAGTTCCCAGGGCTGCCCGAGGCTCTGTCGTTCGTGGCGCTCGTGGACGGCTA
CTTCCGGCTGACCACGGACTCCCAGCACTTCTTCTGCAAGGAGGTGGCACCGCCGAGGCT
GCTGGAGGAAGTGGCCGAGCAGTGCCACGGCCCCATCACTCTGGACTTTGCCATCAACAA
GCTCAAGACTGGGGGCTCACGTCCTGGCTCCTATGTTCTCCGCCGCAGCCCCCAGGACTT
TGACAGCTTCCTCCTCACTGTCTGTGTCCAGAACCCCCTTGGTCCTGATTATAAGGGCTG
CCTCATCCGGCGCAGCCCCACAGGAACCTTCCTTCTGGTTGGCCTCAGCCGACCCCACAG
CAGTCTTCGAGAGCTCCTGGCAACCTGCTGGGATGGGGGGCTGCACGTAGATGGGGTGGC
AGTGACCCTCACTTCCTGCTGTATCCCCAGACCCAAAGAAAAGTCCAACCTGATCGTGGT
CCAGAGAGGTCACAGCCCACCCACATCATCCTTGGTTCAGCCCCAATCCCAATACCAGCT
GAGTCAGATGACATTTCACAAGATCCCTGCTGACAGCCTGGAGTGGCATGAGAACCTGGG
CCATGGGTCCTTCACCAAGATTTACCGGGGCTGTCGCCATGAGGTGGTGGATGGGGAGGC
CCGAAAGACAGAGGTGCTGCTGAAGGTCATGGATGCCAAGCACAAGAACTGCATGGAGTC
ATTCCTGGAAGCAGCGAGCTTGATGAGCCAAGTGTCGTACCGGCATCTCGTGCTGCTCCA
CGGCGTGTGCATGGCTGGAGACAGCACCATGGTGCAGGAATTTGTACACCTGGGGGCCAT
AGACATGTATCTGCGAAAACGTGGCCACCTGGTGCCAGCCAGCTGGAAGCTGCAGGTGGT
CAAACAGCTGGCCTACGCCCTCAACTATCTGGAGGACAAAGGCCTGCCCCATGGCAATGT
CTCTGCCCGGAAGGTGCTCCTGGCTCGGGAGGGGGCTGATGGGAGCCCGCCCTTCATCAA
GCTGAGTGACCCTGGGGTCAGCCCCGCTGTGTTAAGCCTGGAGATGCTCACCGACAGGAT
CCCCTGGGTGGCCCCCGAGTGTCTCCGGGAGGCGCAGACACTTAGCTTGGAAGCTGACAA
GTGGGGCTTCGGCGCCACGGTCTGGGAAGTGTTTAGTGGCGTCACCATGCCCATCAGTGC
CCTGGATCCTGCTAAGAAACTCCAATTTTATGAGGACCGGCAGCAGCTGCCGGCCCCCAA
GTGGACAGAGCTGGCCCTGCTGATTCAACAGTGCATGGCCTATGAGCCGGTCCAGAGGCC
CTCCTTCCGAGCCGTCATTCGTGACCTCAATAGCCTCATCTCTTCAGACTATGAGCTCCT
CTCAGACCCCACACCTGGTGCCCTGGCACCTCGTGATGGGCTGTGGAATGGTGCCCAGCT
CTATGCCTGCCAAGACCCCACGATCTTCGAGGAGAGACACCTCAAGTACATCTCACAGCT
GGGCAAGGGCAACTTTGGCAGCGTGGAGCTGTGCCGCTATGACCCGCTAGGCGACAATAC
AGGTGCCCTGGTGGCCGTGAAACAGCTGCAGCACAGCGGGCCAGACCAGCAGAGGGACTT
TCAGCGGGAGATTCAGATCCTCAAAGCACTGCACAGTGATTTCATTGTCAAGTATCGTGG
TGTCAGCTATGGCCCGGGCCGCCAGAGCCTGCGGCTGGTCATGGAGTACCTGCCCAGCGG
CTGCTTGCGCGACTTCCTGCAGCGGCACCGCGCGCGCCTCGATGCCAGCCGCCTCCTTCT
CTATTCCTCGCAGATCTGCAAGGGCATGGAGTACCTGGGCTCCCGCCGCTGCGTGCACCG
CGACCTGGCCGCCCGAAACATCCTCGTGGAGAGCGAGGCACACGTCAAGATCGCTGACTT
CGGCCTAGCTAAGCTGCTGCCGCTTGACAAAGACTACTACGTGGTCCGCGAGCCAGGCCA
GAGCCCCATTTTCTGGTATGCCCCCGAATCCCTCTCGGACAACATCTTCTCTCGCCAGTC
AGACGTCTGGAGCTTCGGGGTCGTCCTGTACGAGCTCTTCACCTACTGCGACAAAAGCTG
CAGCCCCTCGGCCGAGTTCCTGCGGATGATGGGATGTGAGCGGGATGTCCCCGCCCTCTG
CCGCCTCTTGGAACTGCTGGAGGAGGGCCAGAGGCTGCCGGCGCCTCCTGCCTGCCCTGC
TGAGGTTCACGAGCTCATGAAGCTGTGCTGGGCCCCTAGCCCACAGGACCGGCCATCATT
CAGCGCCCTGGGCCCCCAGCTGGACATGCTGTGGAGCGGAAGCCGGGGGTGTGAGACTCA
TGCCTTCACTGCTCACCCAGAGGGCAAACACCACTCCCTGTCCTTTTCATAGCTCCTGCC
CGCAGACCTCTGGATTAGGTCTCTGTTGACTGGCTGTGTGACCTTAGGCCCGGAGCTGCC
CCTCTCTGGGCCTCAGAGGCCTTATGAGGGTCCTCTACTTCAGGAACACCCCCACGACAT
TGCATTTGGGGGGGCTCCCGTGGCCTGTAGAATAGCCTGTGGCCTTTGCAATTTGTTAAG
GTTCAAGACAGATGGGCATATGTGTCAGTGGGGCTCTCTGAGTCCTGGCCCAAAGAAGCA
AGGAACCAAATTTAAGACTCTCGCATCTTCCCAACCCCTTAAGCCCTGGCCCCCTGAGTT
TCCTTTTCTGTCTCTCTCTTTTTATTTTTTTTATTTTTATTTTTATTTTTGAGACAGAGC
CTCGCTCTGTTACCCAGGGTGGAGTGCAGTGGTGCGATCTCGGCTCAGTGCAACCTCTGC
TTCCCAGGTTCAAGCGATTCTCCTGCCTCAGCCTCCCGAGTAGCTGGGATTACAGGTGTG
CACCACCACACCCGGCTAATTTTTTTTATTTTTAATAGAGATGAGGTTTCACCATGATGG
CCAGGCTGATCTCGAACTCCTAACCTCAAGTGATCCTCCCACCTCAGCCTCCCAAAGTGT
TGGAATAATAGGCATGAGCCACTGCACCCAGGCTTTTTTTTTTTTAAATTTATTATTATT
ATTTTTAAGAGACAGGATCTTGCTACGTTGCCCAGGCTGGTCTTGAACTCCTGGGCTACA
GTGATCCTCCTGCCTTATCCTCCTAAATAGCTGGGACTACAGCACCTAGTTTTGAGTTTC
CTGTCTTATTTCCAATGGGGACATTCATGTAGCTTTTTTTTTTTTTTTTTTTTTGAGACG
GAGTCTCGCTCTGTCGCCCAGGCTGGAGTACAGTGGCGCAATCTAGGCTCACTGCAAGCT
CCGCCTCCTGGGTTCACACCATTCTCTCGCCTCAGCCTCCCAAGTAGCTGGGACTACAGG
CGCCCGCCACCACACCCGGCTAATTTTTTGTATTTTTAGTAGAGACGGGGTTTCACCTTG
TTAGCCAGGATGGTTTCCATCTCCTGACCTCGTGATCTGCCCGTCTCGGCCTCCCAAAGT
GCTGGGATTACAGGCATGAGCCACTGCGCCCGGCCCTCATGTAGCTTTAAATGTATGATC
TGACTTCTGCTCCCCGATCTCTGTTTCTCTGGAGGAAGCCAAGGACAAGAGCAGTTGCTG
TGGCTGGGACTCTGCCTTTTAGGGGAGCCCGTGTATCTCTTTGGGATCCTGAAAGGGGGC
AGGAAAGGCTGGGGTCCCAGTCCACCCTAATGGTATCTGAGTGTCCTAGGGCTTCAGTTT
TCCCACCTGTCCAATGGGACCCTTTCTGTCCTCACCCTACAAGGGGCACAAAGGGATGAC
ACCAAACCTGGCAGGAACTTTTCACGCAATCAAGGGAAGGAAAGGCATTCCTGGCAGAGG
GAACAGCATGCCAAGCGTGAGAAGGCTCAGAGTAAGGAGGTTAAGAGCCCAAGTATTGGA
GCCTACAGTTTTGCCCCTTCCATGCAGTGTGACAGTGGGCAAGTTCCTTTCCCTCTCTGG
GTCTCAGTTCTGTCCCCTGCAAAATGGTCAGAGCTTACCCCTTGGCTGTGCAGGGTCAAC
TTTCTGACTGGTGAGAGGGATTCTCATGCAGGTTAAGCTTCTGCTGCTCCTCCTCACCTG
CAAAGCTTTTCTGCCACTTTTGCCTCCTTGGAAAACTCTTATCCATCTCTCAAAACTCCA
GCTACCACATCCTTGCAGCCTTCCCTCATATACCCCCACTACTACTGTAGCCCTGTCCTT
CCCTCCAGCCCCACTCTGGCCCTGGGGCTGGGGAAGTGTCTGTGTCCAGCTGTCTCCCCT
GACCTCAGGGTTCCTTGGGGGCTGGGCTGAGGCCTCAGTACAGAGGGGGCTCTGGAAATG
TTTGTTGACTGAATAAAGGAATTCAGGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA
AAAAAAAAAAAAAAAAAAAAAAAA
>OriGene 5' read for NM_000215 unedited
CGGCACACGGACGCCAACTAACAAGGGGGCGGCCGCGAACCCCGGGAACTCGGCCCCGCC
CGCCCTCCGAAGCCAGGGCCCCCGCCCGCCAGGACCGGGAGCCCCTCCTCTCCGTGCCCC
GGCAAGCCGCACTCACGGCACCCCCAAGCGAAGAGACGCCCCCGATCCCTCAGCGTTCAT
GCAGCCTCTCGTCCACGGAGGCTGGTGCCCTGCATGTGCTGCTGCCCGCTCGGGGCCCCG
GGCCCCCCCAGCGCCTATCTTTCTCCTTTGGGGACCACTTGGCTGAGGACCTGTGCGTGC
AGGCTGCCAAGGCCAGCGGCATCCTGCCTGTGTACCACTCCCTCTTTGCTCTGGCCACGG
AGGACCTGTCCTGCTGGTTCCCCCCGAGCCACATCTTCTCCGTGGAGGATGCCAGCACCC
AAGTCCTGCTGTACAGGATTCGCTTTTACTTCCCCAATTGGTTTGGGCTGGAGAAGTGCC
ACCGCTTCGGGCTACGCAAGGATTTGGCCAGTGCTATCCTTGACCTGCCAGTCCTGGAGC
ACCTCTTTGCCCAGCACCGCAGTGACCTGGTGAGTGGGCGCCTCCCCGTGGGCCTCAGTC
TCAAGGAGCAGGGTGAGTGTCTCAGCCTGGCCGTGTTGGACCTGGCCCGGATGGCGCGAG
AGCAGGCCCAGCGGCCGGGAGAGCTGCTGAAGACTGTCAGCTACAAGGCCTGCCTACCCC
CAAGCCTGCGCGACCTGATCCAGGGCCTGAGCTTCGTGACGCGGAGGCGTATTCGGAGGA
CGGTGCGCAGAGCCCTGCGCCGCGTGGCCGCTGCCAGGCAGACCGCACTCGCTCATGACA
GTACATCATGACCTGAGCGCTGATCCAGCCGGGCCGCCGAGACCTTTCCACGTGGCCTCC
CT
>Forward primer walk for NM_000215 unedited
NNNCCGTGGCGAGACNAGNCACACGTNCAGGTACGCTGNACTTCGCCTAGCTAAGCTGCT
GCCGCTTGACAAAGACTACTACGTGGTCCGCGAGCCAGGCCAGAGCCCCATTTTCTGGTA
TGCCCCCGAATCCCTCTCGGACAACATCTTCTCTCGCCAGTCAGACGTCTGGAGCTTCGG
GGTCGTCCTGTACGAGCTCTTCACCTACTGCGACAAAAGCTGCAGCCCCTCGGCCGAGTT
CCTGCGGATGATGGGATGTGAGCGGGATGTCCCCGCCCTCTGCCGCCTCTTGGAACTGCT
GGAGGAGGGCCAGAGGCTGCCGGCGCCTCCTGCCTGCCCTGCTGAGGTTCACGAGCTCAT
GAAGCTGTGCTGGGCCCCTAGCCCACAGGACCGGCCATCATTCAGCGCCCTGGGCCCCCA
GCTGGACATGCTGTGGAGCGGAAGCCGGGGGTGTGAGACTCATGCCTTCACTGCTCACCC
AGAGGGCAAACACCACTCCCTGTCCTTTTCATAGCTCCTGCCCGCAGACCTCTGGATTAG
GTCTCTGTTGACTGGCTGTGTGACCTTAGGCCCGGAGCTGCCCCTCTCTGGGCCTCAGAG
GCCTTATGAGGGTCCTCTACTTCAGGAACACCCCCACGACATTGCATTTGGGGGGGCTCC
CGTGGCCTGTAGAATAGCCTGTGGCCTTTGCAATTTGTTAAGGTTCAAGACAGATGGGCA
TATGTGTCAGTGGGGCTCTCTGAGTCCTGGCCCANAAAGCAGGAACCAAATTAAGACTCT
CGCATCTTCCCAACCCCTTAAGCCCTGGCCCCCTGAGTTTCCTTTTCAGGCNCTCTCTTT
TTATTTTTTTATTTTTATTTTTATTTTTGAGACGAGCCTCGCTCTGTTACCCAGGGTGGA
G
Restriction Sites Please inquire     
ACCN NM_000215
Insert Size 5500 bp
OTI Disclaimer Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.

The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
Product Components The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_000215.2, NP_000206.2
RefSeq Size 4025 bp
RefSeq ORF 3375 bp
Locus ID 3718
Cytogenetics 19p13.11
Domains B41, pkinase, SH2, TyrKc, S_TKc
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Chemokine signaling pathway, Jak-STAT signaling pathway, Primary immunodeficiency
Gene Summary 'The protein encoded by this gene is a member of the Janus kinase (JAK) family of tyrosine kinases involved in cytokine receptor-mediated intracellular signal transduction. It is predominantly expressed in immune cells and transduces a signal in response to its activation via tyrosine phosphorylation by interleukin receptors. Mutations in this gene are associated with autosomal SCID (severe combined immunodeficiency disease). [provided by RefSeq, Jul 2008]'

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