DAP5 (EIF4G2) (NM_001042559) Human Untagged Clone

CAT#: SC311411

EIF4G2 (untagged)-Human eukaryotic translation initiation factor 4 gamma, 2 (EIF4G2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EIF4G2
• Synonyms: AAG1; DAP5; NAT1; P97
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001042559 edited
  CACGCCTTAGCGCCAGCAGTGAGTCGGAGCTCTATGGAGGTGGCAGCGGGTACCGAGTGG
  CGGCTGCAGCAGCGACTCCTCTGAGCTGAGTTTGAGGCCGTCCCCGACTCCTTCCTCCCC
  CTTCCCTCCCCCTTTTTTTTGTTTTCCGTTCCCCTTTCCCCTCCCTTCCCTATCCCCGAC
  GACCGGATCCTGAGGAGGCAGCTGCGGTGGCAGCTGCTGAGTTCTCGGTGAAGGTATTTC
  ATTTCTCCTGTCCCCTCCCCTCCCCACCCCATCTATTAATATTATTCTTTTGAAGATTCT
  TCGTTGTCAAGCCGCCAAAGTGGAGAGTGCGATTGCAGAAGGGGGTGCTTCTCGTTTCAG
  TGCTTCTTCGGGCGGAGGAGGAAGTAGGGGTGCACCTCAGCACTATCCCAAGACTGCTGG
  CAACAGCGAGTTCCTGGGGAAAACCCCAGGGCAAAACGCTCAGAAATGGATTCCTGCACG
  AAGCACTAGACGAGATGACAACTCCGCAGCAAACAACTCCGCAAACGAAAAAGAACGACA
  TGATGCAATCTTCAGGAAAGTAAGAGGCATACTAAATAAGCTTACTCCTGAAAAGTTTGA
  CAAGCTATGCCTTGAGCTCCTCAATGTGGGTGTAGAGTCTAAACTCATCCTTAAAGGGGT
  CATACTGCTGATTGTGGACAAAGCCCTAGAAGAGCCAAAGTATAGCTCACTGTATGCTCA
  GCTATGTCTGCGATTGGCAGAAGATGCACCAAACTTTGATGGCCCAGCAGCAGAGGGTCA
  ACCAGGACAGAAGCAAAGCACCACATTCAGACGCCTCCTAATTTCCAAATTACAAGATGA
  ATTTGAAAACCGAACTAGAAATGTTGATGTCTATGATAAGCGTGAAAATCCCCTCCTCCC
  CGAGGAGGAGGAACAGAGAGCCATTGCTAAGATCAAGATGTTGGGAAACATCAAATTCAT
  TGGAGAGCTTGGCAAGCTTGATCTTATTCACGAATCTATCCTTCATAAGTGCATCAAAAC
  ACTTTTGGAAAAGAAGAAGAGAGTCCAACTCAAAGATATGGGAGAGGATTTGGAGTGCCT
  CTGTCAGATAATGAGGACAGTGGGACCTAGATTAGACCATGAACGAGCCAAGTCCTTAAT
  GGATCAGTACTTTGCCCGAATGTGCTCCTTGATGTTAAGTAAGGAATTGCCAGCAAGGAT
  TCGTTTCCTGCTGCAGGATACCGTAGAGTTGCGAGAACACCATTGGGTTCCTCGCAAGGC
  TTTTCTTGACAATGGACCAAAGACGATCAATCAAATTCGTCAAGATGCAGTAAAAGATCT
  AGGGGTGTTTATTCCTGCTCCTATGGCTCAAGGGATGAGAAGTGACTTCTTTCTGGAGGG
  ACCGTTCATGCCACCCAGGATGAAAATGGATAGGGACCCACTTGGAGGACTTGCTGATAT
  GTTTGGACAAATGCCAGGTAGCGGAATTGGTACTGGTCCAGGAGTTATCCAGGATAGATT
  TTCACCCACCATGGGACGTCATCGTTCAAATCAACTCTTCAATGGCCATGGGGGACACAT
  CATGCCTCCCACACAATCGCAGTTTGGAGAGATGGGAGGCAAGTTTATGAAAAGCCAGAT
  TAGCCTGAGGCCTGCTCAGTCGTTCCTAATGAATAAAAATCAAGTGCCAAAGCTTCAGCC
  CCAGATAACTATGATTCCTCCTAGTGCACAACCACCACGCACTCAAACACCACCTCTGGG
  ACAGACACCTCAGCTTGGTCTCAAAACTAATCCACCACTTATCCAGGAAAAGCCTGCCAA
  GACCAGCAAAAAGCCACCACCGTCAAAGGAAGAACTCCTTAAACTAACTGAAACTGTTGT
  GACTGAATATCTAAATAGTGGAAATGCAAATGAGGCTGTCAATGGTGTAAGAGAAATGAG
  GGCTCCTAAACACTTTCTTCCTGAGATGTTAAGCAAAGTAATCATCCTGTCACTAGATAG
  AAGCGATGAAGATAAAGAAAAAGCAAGTTCTTTGATCAGTTTACTCAAACAGGAAGGGAT
  AGCCACAAGTGACAACTTCATGCAGGCTTTCCTGAATGTATTGGACCAGTGTCCCAAACT
  GGAGGTTGACATCCCTTTGGTGAAATCCTATTTAGCACAGTTTGCAGCTCGTGCCATCAT
  TTCAGAGCTGGTGAGCATTTCAGAACTAGCTCAACCACTAGAAAGTGGCACCCATTTTCC
  TCTCTTCCTACTTTGTCTTCAGCAGTTAGCTAAATTACAAGATCGAGAATGGTTAACAGA
  ACTTTTTCAACAAAGCAAGGTCAATATGCAGAAAATGCTCCCAGAAATTGATCAGAATAA
  GGACCGCATGTTGGAGATTTTGGAAGGAAAGGGACTGAGTTTCTTATTCCCACTCCTCAA
  ATTGGAGAAGGAACTGTTGAAGCAAATAAAGTTGGATCCATCCCCTCAAACCATATATAA
  ATGGATTAAAGATAACATCTCTCCCAAACTTCATGTAGATAAAGGATTTGTGAACATCTT
  AATGACTAGCTTCTTACAGTACATTTCTAGTGAAGTAAACCCCCCCAGCGATGAAACAGA
  TTCATCCTCTGCTCCTTCCAAAGAACAGTTAGAGCAGGAAAAACAACTACTACTATCTTT
  CAAGCCAGTAATGCAGAAATTTCTTCATGATCACGTTGATCTACAAGTCAGTGCCCTGTA
  TGCTCTCCAGGTGCACTGCTATAACAGCAACTTCCCAAAAGGCATGTTACTTCGCTTTTT
  TGTGCACTTCTATGACATGGAAATTATTGAAGAAGAAGCTTTCTTGGCTTGGAAAGAAGA
  TATAACCCAAGAGTTTCCGGGAAAAGGCAAGGCTTTGTTCCAGGTGAATCAGTGGCTAAC
  CTGGTTAGAAACTGCTGAAGAAGAAGAATCAGAGGAAGAAGCTGACTAAAGAACCAGCCA
  AAGCCTTAAATTGTGCAAAACATACTGTTGCTATGATGTAACTGCATTTGACCTAACCAC
  TGCGAAAATTCATTCCGCTGTAATGTTTTCACAATATTTAAAGCAGAAGCACGTCAGTTA
  GGATTTCCTTCTGCATAAGGTTTTTTTGTAGTGTAATGTCTTAATCATAGTCTACCATCA
  AATATTTTAGGAGTATCTTTAATGTTTAGATAGTATATTAGCAGCATGCAATAATTACAT
  CATAAGTTCTCAAGCAGAGGCAGTCTATTGCAAGGACCTTCTTTGCTGCCAGTTATCATA
  GGCTGTTTTAAGTTAGAAAACTGAATAGCAACACTGAATACTGTAGAAATGCACTTTGCT
  CAGTAATACTTGAGTTGTTGCAATATTTGATTATCCATTTGGTTGTTACAGAAAAATTCT
  TAACTGTAATTGATGGTTGTTGCCGTAATAGTATATTGCCTGTATTTCTACCTCTAGTAA
  TGGGCTTTATGTGCTAGATTTTAATATCCTTGAGCCTGGGCAAGTGCACAAGTCTTTTTA
  AAAGAAACATGGTTTACTTGCACAAAACTGATCAGTTTTGAGAGATCGTTAATGCCCTTG
  AAGTGGTTTTTGTGGGTGTGAAACAAATGGTGAGAATTTGAATTGGTCCCTCCTATTATA
  GTATTGAAATTAAGTCTACTTAATTTATCAAGTCATGTTCATGCCCTGATTTTATATACT
  TGTATCTATCAATAAACATTGTGATACTTGATGAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAAAAAAAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_001042559
• Insert Size: 3800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_001042559.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042559.1, NP_001036024.1
• RefSeq Size: 3694 bp
• RefSeq ORF: 2610 bp
• Locus ID: 1982
• Cytogenetics: 11p15.4
• Protein Families: Transcription Factors
• Protein Pathways: Viral myocarditis
• Gene Summary: 'Translation initiation is mediated by specific recognition of the cap structure by eukaryotic translation initiation factor 4F (eIF4F), which is a cap binding protein complex that consists of three subunits: eIF4A, eIF4E and eIF4G. The protein encoded by this gene shares similarity with the C-terminal region of eIF4G that contains the binding sites for eIF4A and eIF3; eIF4G, in addition, contains a binding site for eIF4E at the N-terminus. Unlike eIF4G, which supports cap-dependent and independent translation, this gene product functions as a general repressor of translation by forming translationally inactive complexes. In vitro and in vivo studies indicate that translation of this mRNA initiates exclusively at a non-AUG (GUG) codon. Alternatively spliced transcript variants encoding different isoforms of this gene have been described. [provided by RefSeq, Jul 2008]'
  Transcript Variant: This variant (2) lacks an alternate in-frame exon in the central coding region, compared to variant 1, resulting in a shorter isoform (2), compared to isoform 1.