KDM5A (NM_001042603) Human Untagged Clone

CAT#: SC311431

KDM5A (untagged)-Human lysine (K)-specific demethylase 5A (KDM5A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KDM5A
• Synonyms: RBBP-2; RBBP2; RBP2
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001042603 edited
  ATGGCGGGCGTGGGGCCGGGGGGCTACGCGGCGGAGTTCGTGCCACCGCCAGAGTGCCCC
  GTCTTTGAGCCGAGTTGGGAGGAGTTCACAGATCCGCTCAGCTTTATCGGCCGCATCCGG
  CCTTTGGCGGAGAAAACCGGCATCTGCAAAATTCGGCCGCCCAAGGACTGGCAGCCTCCA
  TTTGCCTGTGAAGTAAAAAGCTTTCGTTTCACTCCAAGAGTCCAGCGCCTGAATGAACTT
  GAGGCAATGACCAGAGTGAGATTGGATTTCTTGGATCAACTAGCAAAATTTTGGGAACTT
  CAAGGATCTACTCTGAAGATCCCTGTGGTAGAGAGAAAAATCCTGGATCTGTATGCTTTG
  AGCAAGATTGTTGCCAGCAAAGGAGGTTTTGAAATGGTCACCAAAGAGAAGAAATGGTCT
  AAAGTGGGTAGTCGCTTGGGATATCTGCCAGGAAAAGGAACTGGGTCTCTTTTGAAGTCA
  CATTATGAAAGAATTCTCTACCCATATGAGCTTTTCCAGTCTGGTGTGAGCCTTATGGGT
  GTGCAGATGCCTAATTTAGATCTTAAAGAAAAAGTGGAGCCTGAGGTTCTCAGCACTGAT
  ACCCAAACTTCCCCAGAGCCAGGCACAAGGATGAACATTCTGCCGAAGAGAACAAGACGT
  GTGAAGACTCAGTCAGAATCTGGAGATGTGAGTAGAAACACGGAACTGAAGAAACTTCAG
  ATTTTTGGGGCTGGGCCCAAGGTTGTGGGCTTGGCAATGGGAACAAAAGATAAAGAAGAT
  GAGGTCACCCGAAGACGAAAAGTTACCAACAGGTCAGACGCATTTAACATGCAAATGAGA
  CAACGGAAAGGCACTCTCTCTGTTAACTTTGTTGATCTCTATGTTTGTATGTTTTGTGGT
  CGGGGAAACAATGAAGATAAATTGCTTTTGTGTGATGGATGTGATGACAGCTATCATACA
  TTTTGTCTAATTCCTCCACTACCTGATGTGCCCAAAGGAGACTGGAGGTGTCCTAAATGT
  GTCGCCGAGGAATGTAGCAAACCTCGAGAAGCCTTTGGATTTGAACAAGCTGTACGAGAG
  TATACACTTCAGAGCTTTGGAGAGATGGCAGATAATTTTAAGTCTGATTATTTTAATATG
  CCAGTCCATATGGTTCCCACAGAACTAGTAGAAAAGGAATTTTGGCGGCTGGTAAGCAGC
  ATTGAAGAAGATGTTATTGTGGAATATGGAGCAGATATCTCCTCAAAAGACTTTGGAAGT
  GGATTTCCGGTGAAGGATGGGCGGAGAAAGATTCTGCCAGAAGAAGAGGAATATGCACTT
  TCTGGTTGGAATTTGAATAACATGCCTGTCCTGGAACAGTCTGTTCTTGCACATATTAAT
  GTGGACATCTCTGGTATGAAAGTGCCGTGGCTCTATGTGGGAATGTGCTTCTCTTCTTTT
  TGCTGGCACATTGAGGATCACTGGAGTTATTCCATCAACTACTTGCACTGGGGGGAGCCA
  AAGACATGGTATGGTGTGCCATCTCATGCTGCAGAGCAACTGGAGGAGGTGATGAGAGAG
  CTGGCCCCCGAGTTATTTGAATCCCAGCCTGATCTTCTGCATCAGTTAGTTACCATCATG
  AACCCCAACGTGCTAATGGAGCATGGTGTGCCTGTGTACAGGACCAATCAGTGTGCTGGC
  GAGTTTGTTGTGACATTTCCTCGTGCCTATCACTCTGGATTTAACCAGGGCTACAACTTT
  GCTGAAGCTGTGAACTTCTGTACTGCTGACTGGTTGCCCATTGGACGTCAATGTGTAAAT
  CATTACCGACGCCTAAGGCGCCACTGTGTCTTTTCACACGAGGAACTAATTTTCAAGATG
  GCAGCAGATCCAGAATGCTTAGATGTGGGGCTGGCTGCCATGGTCTGCAAAGAATTGACT
  CTCATGACTGAAGAAGAAACACGATTAAGAGAGTCTGTTGTACAGATGGGTGTCCTGATG
  TCAGAAGAAGAAGTGTTTGAACTTGTTCCTGATGATGAGCGGCAGTGTTCAGCATGCAGA
  ACCACATGTTTTCTCTCTGCTCTCACATGTTCCTGTAATCCTGAGCGGCTTGTATGTCTC
  TACCATCCAACTGATCTGTGCCCCTGCCCCATGCAGAAGAAATGTCTTAGATATCGCTAC
  CCATTAGAAGACCTCCCTTCTCTGCTATATGGTGTAAAAGTCAGGGCACAGTCCTATGAC
  ACTTGGGTCAGTCGTGTTACAGAAGCATTGTCTGCTAACTTCAACCACAAAAAAGATTTG
  ATTGAATTGCGAGTATTGCTGGAAGATGCTGAGGATAGGAAATACCCAGAGAATGATCTC
  TTTCGAAAACTCAGGGATGCTGTAAAAGAAGCTGAGACCTGTGCTTCTGTGGCTCAGCTG
  CTTCTGAGCAAAAAGCAGAAACACAGACAGAGCCCAGATAGTGGGAGGACTCGGACCAAA
  CTGACAGTGGAAGAATTGAAGGCCTTTGTCCAACAACTTTTTAGTCTTCCGTGTGTCATC
  AGCCAAGCTCGGCAAGTAAAGAATCTGCTAGATGATGTGGAAGAGTTTCATGAACGTGCT
  CAGGAGGCCATGATGGATGAAACCCCAGATTCTTCCAAACTCCAGATGTTGATAGATATG
  GGCTCTAGTCTCTATGTGGAACTCCCTGAATTACCACGACTGAAGCAAGAGCTACAACAG
  GCTCGGTGGTTGGACGAAGTAAGACTGACCTTATCAGATCCGCAACAAGTCACTTTGGAT
  GTCATGAAGAAGCTGATAGACTCTGGGGTAGGGTTGGCACCCCACCATGCTGTGGAGAAA
  GCAATGGCTGAACTACAGGAGCTCCTTACAGTCTCTGAACGATGGGAAGAAAAGGCTAAG
  GTCTGCCTACAGGCAAGACCGAGGCACAGTGTGGCAAGTTTAGAAAGCATTGTGAATGAA
  GCCAAGAACATTCCAGCCTTTCTACCCAATGTGTTGTCCTTGAAAGAAGCCTTACAAAAG
  GCTCGAGAATGGACCGCTAAAGTGGAAGCTATTCAGAGTGGCAGCAATTACGCTTATTTG
  GAGCAGCTTGAGAGCTTGTCTGCGAAAGGACGCCCTATTCCTGTGCGTCTTGAAGCACTG
  CCGCAAGTGGAATCACAGGTAGCAGCAGCACGGGCATGGAGAGAACGGACTGGGCGGACG
  TTTCTTAAGAAGAATTCTAGCCATACATTGTTACAGGTGCTGAGCCCCCGGACCGACATT
  GGTGTATATGGGAGTGGCAAAAATAGGAGGAAAAAAGTAAAAGAACTAATAGAAAAAGAA
  AAAGAAAAGGATCTGGACCTGGAGCCTCTGAGTGATCTGGAGGAAGGATTGGAGGAAACC
  AGAGATACAGCCATGGTGGTGGCAGTTTTCAAAGAACGGGAGCAAAAAGAGATTGAAGCC
  ATGCATTCTCTCAGAGCAGCCAACCTAGCCAAGATGACAATGGTGGACCGCATAGAAGAA
  GTAAAATTTTGCATTTGCCGCAAGACAGCCAGTGGGTTTATGCTACAGTGTGAGCTCTGC
  AAAGACTGGTTCCATAACAGCTGTGTTCCTCTTCCTAAATCAAGTTCCCAAAAAAAAGGA
  TCCAGCTGGCAAGCTAAAGAAGTAAAATTCCTTTGCCCTCTTTGTATGCGGTCTCGAAGG
  CCCAGGCTAGAGACTATTCTGTCACTCCTGGTATCCCTTCAGAAGTTGCCCGTACGGTTG
  CCTGAAGGAGAGGCCCTGCAGTGTTTGACAGAACGTGCTATGAGTTGGCAAGATAGAGCG
  CGGCAGGCTCTAGCCACAGATGAACTATCCTCTGCCCTGGCCAAACTATCTGTGTTGAGC
  CAGCGTATGGTGGAACAGGCGGCTCGAGAAAAAACTGAAAAGATCATCAGTGCAGAACTC
  CAAAAAGCAGCTGCCAATCCAGACTTACAGGGACACTTACCTAGTTTCCAGCAGTCTGCT
  TTTAACCGGGTGGTGAGCAGTGTGTCATCTTCTCCTCGACAAACAATGGACTATGATGAT
  GAAGAAACAGACTCTGATGAAGACATTCGAGAGACATATGGCTACGACATGAAGGACACA
  GCCAGTGTGAAGTCCTCTAGTAGTCTTGAACCCAATCTTTTTTGTGATGAAGAGATTCCC
  ATCAAATCTGAGGAGGTGGTGACCCACATGTGGACAGCACCTTCATTTTGTGCAGAGCAT
  GCTTATTCTTCTGCTTCTAAGAGTTGTTCTCAAGGTTCTAGCACCCCAAGGAAACAACCT
  CGGAAGAGCCCTTTGGTGCCCCGAAGTTTGGAACCTCCAGTGCTGGAGTTGTCACCTGGA
  GCTAAGGCACAACTGGAAGAACTTATGATGGTTGGAGATCTACTGGAAGTATCTCTGGAC
  GAGACTCAACACATATGGCGGATTTTGCAGGCCACACACCCACCCTCTGAAGACAGATTC
  TTGCATATCATGGAGGATGACAGCATGGAAGAGAAACCACTAAAAGTGAAAGGAAAGGAC
  TCTTCAGAGAAGAAACGGAAACGGAAGCTAGAAAAGGTAGAGCAACTTTTTGGAGAAGGA
  AAACAGAAGTCCAAGGAGTTAAAGAAAATGGACAAACCTAGAAAGAAGAAATTAAAATTA
  GGTGCAGACAAATCAAAGGAGCTGAATAAACTGGCCAAGAAACTAGCAAAAGAAGAAGAG
  AGAAAGAAAAAGAAGGAGAAGGCTGCTGCAGCCAAAGTTGAACTTGTGAAAGAGAGCACT
  GAAAAGAAAAGAGAGAAAAAGGTGCTGGACATCCCCTCAAAGTATGACTGGTCAGGAGCA
  GAGGAGTCTGATGATGAGAATGCTGTGTGCGCAGCACAGAACTGCCAAAGGCCCTGCAAG
  GACAAGGTAGACTGGGTACAATGTGATGGTGGCTGTGATGAGTGGTTTCATCAAGTTTGT
  GTGGGTGTATCTCCAGAAATGGCTGAAAATGAAGATTACATCTGTATAAACTGTGCAAAG
  AAGCAGGGGCCAGTTAGCCCAGGTCCAGCACCACCTCCTTCCTTCATAATGAGCTACAAA
  CTACCAATGGAGGATCTTAAAGAGACCAGTTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_001042603
• Insert Size: 6500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this clone has been fully sequenced and found three SNPs within the protein associated with this reference, NM_001042603.1.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042603.1, NP_001036068.1
• RefSeq Size: 10835 bp
• RefSeq ORF: 5073 bp
• Locus ID: 5927
• Cytogenetics: 12p13.33
• Protein Families: Druggable Genome
• Gene Summary: 'This gene encodes a member of the Jumonji, AT-rich interactive domain 1 (JARID1) histone demethylase protein family. The encoded protein plays a role in gene regulation through the histone code by specifically demethylating lysine 4 of histone H3. The encoded protein interacts with many other proteins, including retinoblastoma protein, and is implicated in the transcriptional regulation of Hox genes and cytokines. This gene may play a role in tumor progression. [provided by RefSeq, Aug 2013]'
  Transcript Variant: This variant (1) represents the shorter transcript but encodes the longer isoform (1).