PIK3CD (NM_005026) Human Untagged Clone

CAT#: SC311495

PIK3CD (untagged)-Human phosphoinositide-3-kinase, catalytic, delta polypeptide (PIK3CD)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIK3CD
• Synonyms: APDS; IMD14; p110D; P110DELTA; PI3K
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_005026 edited
  CCGCCCAGCCCTGCCAGCTGCGCCGGGACGATAAGGAGTCAGGCCAGGGCGGGATGACAC
  TCATTGATTCTAAAGCATCTTTAATCTGCCAGGCGGAGGGGGCTTTGCTGGTCTTTCTTG
  GACTATTCCAGAGAGGACAACTGTCATCTGGGAAGTAACAACGCAGGATGCCCCCTGGGG
  TGGACTGCCCCATGGAATTCTGGACCAAGGAGGAGAATCAGAGCGTTGTGGTTGACTTCC
  TGCTGCCCACAGGGGTCTACCTGAACTTCCCTGTGTCCCGCAATGCCAACCTCAGCACCA
  TCAAGCAGCTGCTGTGGCACCGCGCCCAGTATGAGCCGCTCTTCCACATGCTCAGTGGCC
  CCGAGGCCTATGTGTTCACCTGCATCAACCAGACAGCGGAGCAGCAAGAGCTGGAGGACG
  AGCAACGGCGTCTGTGTGACGTGCAGCCCTTCCTGCCCGTCCTGCGCCTGGTGGCCCGTG
  AGGGCGACCGCGTGAAGAAGCTCATCAACTCACAGATCAGCCTCCTCATCGGCAAAGGCC
  TCCACGAGTTTGACTCCTTGTGCGACCCAGAAGTGAACGACTTTCGCGCCAAGATGTGCC
  AATTCTGCGAGGAGGCGGCCGCCCGCCGGCAGCAGCTGGGCTGGGAGGCCTGGCTGCAGT
  ACAGTTTCCCCCTGCAGCTGGAGCCCTCGGCTCAAACCTGGGGGCCTGGTACCCTGCGGC
  TCCCGAACCGGGCCCTTCTGGTCAACGTTAAGTTTGAGGGCAGCGAGGAGAGCTTCACCT
  TCCAGGTGTCCACCAAGGACGTGCCGCTGGCGCTGATGGCCTGTGCCCTGCGGAAGAAGG
  CCACAGTGTTCCGGCAGCCGCTGGTGGAGCAGCCGGAAGACTACACGCTGCAGGTGAACG
  GCAGGCATGAGTACCTGTATGGCAGCTACCCGCTCTGCCAGTTCCAGTACATCTGCAGCT
  GCCTGCACAGTGGGTTGACCCCTCACCTGACCATGGTCCATTCCTCCTCCATCCTCGCCA
  TGCGGGATGAGCAGAGCAACCCTGCCCCCCAGGTCCAGAAACCGCGTGCCAAACCACCTC
  CCATTCCTGCGAAGAAGCCTTCCTCTGTGTCCCTGTGGTCCCTGGAGCAGCCGTTCCGCA
  TCGAGCTCATCCAGGGCAGCAAAGTGAACGCCGACGAGCGGATGAAGCTGGTGGTGCAGG
  CCGGGCTTTTCCACGGCAACGAGATGCTGTGCAAGACGGTGTCCAGCTCGGAGGTGAGCG
  TGTGCTCGGAGCCCGTGTGGAAGCAGCGGCTGGAGTTCGACATCAACATCTGCGACCTGC
  CCCGCATGGCCCGTCTCTGCTTTGCGCTGTACGCCGTGATCGAGAAAGCCAAGAAGGCTC
  GCTCCACCAAGAAGAAGTCCAAGAAGGCGGACTGCCCCATTGCCTGGGCCAACCTCATGC
  TGTTTGACTACAAGGACCAGCTTAAGACCGGGGAACGCTGCCTCTACATGTGGCCCTCCG
  TCCCAGATGAGAAGGGCGAGCTGCTGAACCCCACGGGCACTGTGCGCAGTAACCCCAACA
  CGGATAGCGCCGCTGCCCTGCTCATCTGCCTGCCCGAGGTGGCCCCGCACCCCGTGTACT
  ACCCCGCCCTGGAGAAGATCTTGGAGCTGGGGCGACACAGCGAGTGTGTGCATGTCACCG
  AGGAGGAGCAGCTGCAGCTGCGGGAAATCCTGGAGCGGCGGGGGTCTGGGGAGCTGTATG
  AGCACGAGAAGGACCTGGTGTGGAAGCTGCGGCATGAAGTCCAGGAGCACTTCCCGGAGG
  CGCTAGCCCGGCTGCTGCTGGTCACCAAGTGGAACAAGCATGAGGATGTGGCCCAGATGC
  TCTACCTGCTGTGCTCCTGGCCGGAGCTGCCCGTCCTGAGCGCCCTGGAGCTGCTAGACT
  TCAGCTTCCCCGATTGCCACGTAGGCTCCTTCGCCATCAAGTCGCTGCGGAAACTGACGG
  ACGATGAGCTGTTCCAGTACCTGCTGCAGCTGGTGCAGGTGCTCAAGTACGAGTCCTACC
  TGGACTGCGAGCTGACCAAATTCCTGCTGGACCGGGCCCTGGCCAACCGCAAGATCGGCC
  ACTTCCTTTTCTGGCACCTCCGCTCCGAGATGCACGTGCCGTCGGTGGCCCTGCGCTTCG
  GCCTCATCCTGGAGGCCTACTGCAGGGGCAGCACCCACCACATGAAGGTGCTGATGAAGC
  AGGGGGAAGCACTGAGCAAACTGAAGGCCCTGAATGACTTCGTCAAGCTGAGCTCTCAGA
  AGACCCCCAAGCCCCAGACCAAGGAGCTGATGCACTTGTGCATGCGGCAGGAGGCCTACC
  TAGAGGCCCTCTCCCACCTGCAGTCCCCACTCGACCCCAGCACCCTGCTGGCTGAAGTCT
  GCGTGGAGCAGTGCACCTTCATGGACTCCAAGATGAAGCCCCTGTGGATCATGTACAGCA
  ACGAGGAGGCAGGCAGCGGCGGCAGCGTGGGCATCATCTTTAAGAACGGGGATGACCTCC
  GGCAGGACATGCTGACCCTGCAGATGATCCAGCTCATGGACGTCCTGTGGAAGCAGGAGG
  GGCTGGACCTGAGGATGACCCCCTATGGCTGCCTCCCCACCGGGGACCGCACAGGCCTCA
  TTGAGGTGGTACTCCGTTCAGACACCATCGCCAACATCCAACTCAACAAGAGCAACATGG
  CAGCCACAGCCGCCTTCAACAAGGATGCCCTGCTCAACTGGCTGAAGTCCAAGAACCCGG
  GGGAGGCCCTGGATCGAGCCATTGAGGAGTTCACCCTCTCCTGTGCTGGCTATTGTGTGG
  CCACATATGTGCTGGGCATTGGCGATCGGCACAGCGACAACATCATGATCCGAGAGAGTG
  GGCAGCTGTTCCACATTGATTTTGGCCACTTTCTGGGGAATTTCAAGACCAAGTTTGGAA
  TCAACCGCGAGCGTGTCCCATTCATCCTCACCTACGACTTTGTCCATGTGATTCAGCAGG
  GGAAGACTAATAATAGTGAGAAATTTGAACGGTTCCGGGGCTACTGTGAAAGGGCCTACA
  CCATCCTGCGGCGCCACGGGCTTCTCTTCCTCCACCTCTTTGCCCTGATGCGGGCGGCAG
  GCCTGCCTGAGCTCAGCTGCTCCAAAGACATCCAGTATCTCAAGGACTCCCTGGCACTGG
  GGAAAACAGAGGAGGAGGCACTGAAGCACTTCCGAGTGAAGTTTAACGAAGCCCTCCGTG
  AGAGCTGGAAAACCAAAGTGAACTGGCTGGCCCACAACGTGTCCAAAGACAACAGGCAGT
  AGTGGCTCCTCCCAGCCCTGGGCCCAAGAGGAGGCGGCTGCGGGTCGTGGGGACCAAGCA
  CATTGGTCCTAAAGGGGCTGAAGAGCCTGAACTGCACCTAACGGGAAAGAACCGACATGG
  CTGCCTTTTGTTTACACTGGTTATTTATTTATGACTTGAAATAGTTTAAGGAGCTAAACA
  GCCATAAACGGAAACGCCTCCTTCATGCAGCGGCGGTGCTGGGCCCCCCGAGGCTGCACC
  TGGCTCTCGGCTGAGGATTGTCACCCCAAGTCTTCCAGCTGGTGGATCTGGGCCCAGCAA
  AGACTGTTCTCCTCCCGAGGGAACCTTCTTCCCAGGCCTCCCGCCAGACTGCCTGGGTCC
  TGGCGCCTGGCGGTCACCTGGTGCCTACTGTCCGACAGGATGCCTCGATCCTCGTGCGAC
  CCACCCTGTGTATCCTCCCTAGACTGAGTTCTGGCAGCTCCCCGAGGCAGCCGGGGTACC
  CT
  
• Restriction Sites: Please inquire
• ACCN: NM_005026
• Insert Size: 3800 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_005026.2.
• Product Components: The cDNA clone is shipped in a 2-D bar-coded Matrix tube as dried plasmid DNA. The package also includes 100 pmols of both the corresponding 5' and 3' vector primers in separate vials. Every lot of primer is tested to provide clean sequencing of OriGene TrueClones.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005026.2, NP_005017.2
• RefSeq Size: 5220 bp
• RefSeq ORF: 3135 bp
• Locus ID: 5293
• Cytogenetics: 1p36.22
• Protein Families: Druggable Genome
• Protein Pathways: Acute myeloid leukemia, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, ErbB signaling pathway, Fc epsilon RI signaling pathway, Fc gamma R-mediated phagocytosis, Focal adhesion, Glioma, Inositol phosphate metabolism, Insulin signaling pathway, Jak-STAT signaling pathway, Leukocyte transendothelial migration, Melanoma, mTOR signaling pathway, Natural killer cell mediated cytotoxicity, Neurotrophin signaling pathway, Non-small cell lung cancer, Pancreatic cancer, Pathways in cancer, Phosphatidylinositol signaling system, Progesterone-mediated oocyte maturation, Prostate cancer, Regulation of actin cytoskeleton, Renal cell carcinoma, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway, Type II diabetes mellitus, VEGF signaling pathway
• Gene Summary: 'Phosphoinositide 3-kinases (PI3Ks) phosphorylate inositol lipids and are involved in the immune response. The protein encoded by this gene is a class I PI3K found primarily in leukocytes. Like other class I PI3Ks (p110-alpha p110-beta, and p110-gamma), the encoded protein binds p85 adapter proteins and GTP-bound RAS. However, unlike the other class I PI3Ks, this protein phosphorylates itself, not p85 protein.[provided by RefSeq, Jul 2010]'